scholarly journals Comparative Immunogenicity Evaluation of Two Commonly Used Infectious Bursal Disease Vaccines in Broiler Chickens in Ethiopia

2021 ◽  
Author(s):  
Likelesh Legese ◽  
Berhane Wakjira ◽  
Tsedale Teshome ◽  
Dereje Nigussie Woldemichael ◽  
Hika Waktole ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Immunological Response ◽  
Elisa Test ◽  
Infectious Bursal Disease ◽  
Differential Leukocyte Count ◽  
Endemic Diseases ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Group 2 ◽  
Group 1

Infectious bursal disease (IBD) is one of the most endemic diseases of commercial poultry in Ethiopia. Vaccination has been practiced as the major means of IBD prevention and control. A study was conducted to determine and compare the immunogenicity of two commercially available IBD vaccines in broiler chicken with maternally derived antibody (MDA). Day-old chickens of 270 were randomly assigned to three groups, group 1 vaccinated with brand 1 vaccine at 7th and 19th days and group 2 with brand 2 vaccine at 15th and 22nd days while group 3 were kept as control. Six chickens were also randomly selected and bled on day 1 for differential leukocyte count (DLC) and determination of MDA. Representative chickens from each group were bled at 24th and 42nd days of age for antibody titration using the indirect ELISA test. DLC scores were determined in the 1st and 24th days. The result revealed highly significant differences (P = 0.001) between group 1 and group 2 in DLC at 24th days of age. Antibody titers against IBD were differed significantly (P = 0.02) at 24th and 42nd days of age in broilers vaccinated with brand 1 and brand 2 vaccines. It is concluded that although both brands of vaccine induce an adequate immunological response at the end of the experiment, brand 1 vaccine has shown significantly high antibody titers against the IBDV and DLC than brand 2.

scholarly journals The Effect of Licorice roots powder on experimental Infection of infectious bursal disease in broiler chickens

2006 ◽  
Vol 30 (2) ◽  
pp. 65-78
Author(s):  
A. K. Kassab
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Food Intake ◽  
Water Content ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Age Group ◽  
Bursal Disease ◽  
Group 3 ◽  
Group 1

This study was conducted to investigate the effect of Licorice roots powder for 35 days in reducing the severity of infection with infectious bursal disease (IBD)and the immune response and performance of broiler chicks .A Total of 150 day old broiler chickens were divided into 5 groups and treated as follows : Group 1: infected with IBD virus at 21 days of age. Group 2: infected with IBD virus at 21 days of age and given 1 gm of Licorice roots powder /1 L of drinking water one day after infection . Group 3 : given 1 gm Licorice roots powder / 1L of drinking water at day one of age and infected with IBD virus at day 21 of age . Group 4:given 1 gm Licorice roots / 1 L of drinking water at 22 days of age .Group 5: untreated control. Antibody (Ab) of IBD titer was measured by ELIZA , the serum total protein (STP) the serum Globulin(SG)and the blood picture were determined , the water content of the droppings was measured , the body weight , food intake was recorded, there was increase (P<0.01) in the (Ab)titer of IBD virus in the group3 comparing with the other groups , the bursa / body weight index (B:BW)three days after infection was increased (P<0.01) in group1 , the STP , the SG , the PCV and RBCs was increased (P<0.01) in group 3 at age 27 days the food intake (FI) Food conversion ratio (FCR) and body weight (BW) in Group 3 were significantly increased , the dropping water content was significantly (P<0 .01)increased in group 3 in comparison with other groups at the period from 23-27 days of age, the morbidity and Mortality rate were significantly lower in group 3 and then 2 in comparison with group 1 (50 % , 63.3 %, 90 % ) and (6.6 % , 16.6 % ,30%) respectively

scholarly journals Subclinical Infectious Bursal Disease in an Integrated Broiler Production Operation

1992 ◽  
Vol 4 (4) ◽  
pp. 406-411 ◽  
Author(s):  
Bruce L. Homer ◽  
Gary D. Butcher ◽  
Richard D. Miles ◽  
Alfredo F. Rossi
Keyword(s):  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Production Operation ◽  
Field Exposure ◽  
Vaccination Programs ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Lymphoid Depletion ◽  
Antibody Levels

A field study was designed to determine the prevalence of subclinical infectious bursal disease (IBD) in broiler chickens from a commercial poultry company. Bursae of Fabricius (BF) from two vaccinated and three nonvaccinated broiler flocks were evaluated histologically, and antibody profiles of these broiler and matched parent breeder flocks were established. Lesions of IBD, including lymphoid necrosis, stromal edema, and infiltrates of heterophils and macrophages, were first detected in BF at 24 days of age in both vaccinated and nonvaccinated chickens. At 41 days, all BF had lesions characteristic of IBD, including severe lymphoid depletion, proliferation of epithelial cells, and mild fibroplasia. Although mean maternal antibody levels (measured by enzyme-linked immunosorbent assay) in broilers were apparently protective through day 12, IBD antibodies decreased to nonprotective levels (below 1,000) by day 16 or 20. Titers began to increase by day 28 or 32 because of field exposure. Sentinel birds, placed with broiler flocks, also developed IBD antibody titers. Broiler breeders had low and nonuniform antibody titers. Prevalence of field IBD exposure was high, and existing vaccination programs were not effective.

scholarly journals Histopathological And Immune Response Against Infectious Bursal Disease In Chickens Vaccinated Against Newcastle Disease

2013 ◽  
Vol 12 (1) ◽  
pp. 66 ◽  
Author(s):  
A.Z. AL Zubeedy
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Elisa Test ◽  
Infectious Bursal Disease ◽  
High Antibody ◽  
Pathological Changes ◽  
Post Vaccination ◽  
Vaccination Programs ◽  
Bursal Disease ◽  
Antibody Titers

Two different vaccination programs against chicken infectious bursal disease (IBD) were compared. Results showed the first program including IBD vaccination at 8 and 16th day old as well as ND vaccination(live attenuated and inactivated types ) give high antibody titers at 21 days post vaccination with significantly difference (P≤0.0005) in comparison with the second program including only IBD vaccine . serological antibodies titer were determined to study the correlation between two different programs using ELISA test. pathological changes in vaccinated groups reveals mild & moderate lesions appear in liver, spleen and bursa of fabriciusKey word : IBD,ND , vaccination , ELISA test ,Broiler , histopathology .

scholarly journals The immune response of broiler chickens fed diets supplemented with Propolis and Digestarom under heat stress condition

2019 ◽  
Vol 42 (2) ◽  
pp. 33-40
Author(s):  
Hasanain shauqi H.
Keyword(s):  
Immune Response ◽  
Heat Stress ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Infectious Bursal Disease ◽  
Stress Group ◽  
Lymphocyte Ratio ◽  
Bursal Disease ◽  
Antibody Titers ◽  
Group 3

                                                             Summary    This research was carried out to evaluate the effect of dietary supplementation  of a mixture of  propolis and phytogenic (Digestarom)on immune responses against Newcastle disease and infectious bursal disease , also Heterophil/lymphocyte ratios in vaccinated broiler under thermoneutral (maintained on usual heat program)and heat stress maintained in  )33 ± 2°C). total of three hundred, one-day-old broilers (Rose 308) chicks were distributed equally in two separated room, thermoneutral  groups and heat stress HS groups along the duration of the experiment (42)day, then each group subdivided in to five groups( 30 chicks for each )  as follow: (thermoneutral group and heat stress group )fed a basal diet supplemented with propolis (2g/kg of diet),(thermoneutral group 2and heat stress2)fed Digestarom(150mg/kg).( thermoneutral  group 3 and heat stress group 3) fed a mixture of (propolis2g/kg +Digestarom150mg/kg) and two control groups without additive. Antibody titers against Newcastle disease  and infectious bursal disease   were measured and Heterophil/lymphocyte  ratio was estimated. In heat stress  chickens the results revealed a significant decrease(P<0.05)in immune response with significant increased(P<0.05) in Heterophil/lymphocyte  ratio, while higher significant (P<0.05)in antibody titers with significant decrease (P<0.05) in Heterophil/lymphocyte  ratio was showed in all thermoneutral chickens but more significant in thermoneutral group 3 and heat stress group 3 groups were gave the best values in comparing with others groups. The role of Propolis or phytogenic on broiler health and immunity had already been reported, but in this study was reported the effects of their mixture on ameliorating  deleterious effects of heat stress.

scholarly journals Transmissible Viral Proventriculitis Caused by Chicken Proventricular Necrosis Virus Displaying Serological Cross-Reactivity with IBDV

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 8
Author(s):  
Marcin Śmiałek ◽  
Michał Gesek ◽  
Daria Dziewulska ◽  
Jowita Samanta Niczyporuk ◽  
Andrzej Koncicki
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Elisa Test ◽  
Cross Reactivity ◽  
Infected Group ◽  
Feed Conversion ◽  
Necrosis Virus ◽  
Bursal Disease ◽  
Group A ◽  
Potential Factor

Transmissible viral proventriculitis (TVP) of chickens is manifested in decreased body weight gains, poor feed conversion and weight diversity. Although TVP etiology has not been defined, a Birnaviridae family member, named chicken proventricular necrosis virus (CPNV) is considered as a potential factor of a disease. This study was undertaken in order to reproduce TVP and to evaluate its etiology. Broiler chickens of the TVP-infected group were inoculated with TVP positive proventriculi homogenate on the 24th day of life. Samples were collected, on infection day and 14 days post-infection (dpi). The 14 dpi anatomo- and histopathological evaluation, revealed that we have succeeded to reproduce TVP. TVP-infected birds gained 30.38% less body weight. In the TVP-infected group a seroconversion against picornaviruses, fowl adenoviruses (FAdV) and infectious bursal disease viruses (IBDV) was recorded with an ELISA test. Using RT-PCR and PCR, CPNV was detected in proventriculi and FAdV in spleens and livers of infected birds, 14 dpi. Our study supports that CPNV is involved in the development of TVP. We did not record the presence of IBDV in TVP or control birds, despite our recording of a seroconversion against IBDV in TVP infected birds. CPNV and IBDV belong to the same family, which allows us to assume serological cross-reactivity between them. The role of FAdV needs further evaluation.

scholarly journals Evaluation of the TGS TA system for the detection of anti-rubella antibodies

2017 ◽  
Vol 32 (2) ◽  
Author(s):  
Olivia Arpino ◽  
Annalisa Cianflone ◽  
Maria Teresa Manco ◽  
Alessia Paganini ◽  
Massimo De Paschale ◽  
...  
Keyword(s):  
Elisa Test ◽  
Childbearing Age ◽  
Past Infection ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Igg Avidity ◽  
Group 3 ◽  
Group 2 ◽  
Sera Samples ◽  
Group 1

<p><em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting antirubella IgG and IgM antibodies and IgG avidity. TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and IgM antibodies. Only in positive IgM samples (retrospective study), TGS TA system was compared to an ELFA IgM test and with an ELISA test for the IgG avidity (if existent).<br /><em>Materials and methods:</em> Two hundred and seventy six sera samples from women were examined with TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 112 were of childbearing age and non-immune women (Group 1), 106 were pregnant with past infection or vaccinated (Group 2) and 49 were pregnant with positive or equivocal IgM (Group 3). <br /><em>Results</em>: The overall concordance of the IgG results between LIAISON XL and TGS TA was 93.3%: 86.6% in Group 1, 97.2% in Group 2 and 100% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 89.0%: 100% in Group 1, 100% in Group 2 and 35.6% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the ELISA and TGS TA tests was 85.7%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 97.4%: 86.6% in Group 1, 97.2% in Group 2 and 85.7% in Group 3. <br /><em>Conclusions</em>: TGA TS system shows to be a valuable tool with overall good clinical correlation and able to clearly identify nonspecific subjects, those with a non-recent infection or those who are vaccinated. The TGS TA test also seems to be especially sensitive in indicating vaccinated subjects with low IgG levels as immune.</p>

scholarly journals Studi Lapang: Penegakan Diagnosis Infectious Bursal Disease (IBD) Pada Ayam Broiler

2019 ◽  
Vol 3 (1) ◽  
pp. 25-30
Author(s):  
Aan Awaludin ◽  
Yudhi Ratna Nugraheni ◽  
Theo Mahiseta Syahniar ◽  
Dyah Laksito Rukmi ◽  
Agus Hadi Prayitno ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Care Management ◽  
Clinical Symptoms ◽  
Viral Disease ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Commercial Farms ◽  
The One ◽  
Organ Changes

Infectious Bursal Disease (IBD), also called Gumboro, was disease which attacked cells in the bursa of fabricius, causing interference with the chicken's immune system or immunosuppressive. IBD was the one of viral disease that often attacks chickens in the field. The aim of this study was to determine the diagnosis of IBD by through at clinical symptoms and necropsy that can still be relevant on in the field. The diagnosis of IBD correctly, cheaply, easily and quickly in the field is very important to optimize the health care management and evaluation program. The method used was by observing clinical symptoms of broiler chickens in commercial farms suspected of contracting IBD and observing post-death organs (necropsy). The object of necropsy was 5 samples of broiler chickens from the farm. Data was analyzed descriptively. The results of this study was the broiler chickens that infected with IBD could be diagnosed through clinical symptoms and post-death organ changes, so that the diagnosis for IBD cases in the field used the observation of clinical symptoms and necropsy are still relevant.

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