scholarly journals Prevalence of Argulus sp. in Indian Major Carps from Bhangore block of South 24 Parganas District, West Bengal, India

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Koel Bhattacharya Sanyal
Keyword(s):  
Fresh Water ◽  
Molecular Identification ◽  
18S Rdna ◽  
West Bengal ◽  
Point Of View ◽  
Economic Point ◽  
Indian Major Carps ◽  
Rdna Analysis ◽  
Major Carps

The study was carried out to find the prevalence and severity of Argulus sp. in Indian Major Carps (IMC) collected from Bhangore block of South 24 Parganas district of West Bengal during November’2018 to August’2019. The highest parasitic prevalence (PFI, 83.33%) had been recorded in the month of January and lowest (PFI, 9%) in the month of August. The identities of selected parasites were further confirmed by molecular identification through 18S rDNA analysis. The study revealed that Argulus infestation was of great implications from economic point of view throughout the study period especially in winter and is the most prevalent problem in fresh water aquaculture systems.

scholarly journals Prevalence of Ectoparasitic Infestation in Indian Major Carps During Winter at Different Blocks of South 24-Parganas District, West Bengal, India

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Koel Bhattacharya Sanyal
Keyword(s):  
Dissolved Oxygen ◽  
West Bengal ◽  
Monsoon Season ◽  
Catla Catla ◽  
Key Factors ◽  
Indian Major Carps ◽  
Post Monsoon Season ◽  
Lower Temperature ◽  
Unfavorable Condition ◽  
Major Carps

The study was carried out to find the prevalence of ectoparasites in carp species specifically Indian Major Carps (IMC) during the post monsoon season (November’16 to March’17). Four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from a total 500 number of  carp species like, Labeo rohita, Catla catla, Cirrhinus mrigala and Labeo calbasu collected from different ponds of selected blocks of South 24-Parganas district of West Bengal. The highest prevalence (64.8%) of infestation had been recorded by Myxozoans and the lowest was by Monogeneans (4.8%). The highest and lowest ectoparasitic prevalence in carp was observed in L. rohita (32.9%) and C. catla (27.3%). Beside these, lower temperature (Average 19.3oC), low pH (Average 6.9) and marginal level of dissolved oxygen (Average 6.0ppm) were also created an unfavorable condition for parasitic infestation during this season. At the end of this experiment it was concluded that disease occurrences due to ectoparasites was high in winter with some key factors like temperature, pH and dissolved oxygen (DO).

scholarly journals Prevalence of Parasitic Diseases of Indian Major Carps in Selected Districts of West Bengal, India

2018 ◽  
Vol 7 (06) ◽  
pp. 2784-2805 ◽  
Author(s):  
G. Vijaysundardeva ◽  
Gadadhar Dash ◽  
Kurva Raghu Ramudu ◽  
T.J. Abraham ◽  
K.U. Shyam
Keyword(s):  
West Bengal ◽  
Parasitic Diseases ◽  
Indian Major Carps ◽  
Major Carps

scholarly journals ANTIBIOTIC-RE¬¬SISTANT MOTILE AEROMONADS ASSOCIATED WITH CULTURED INDIAN MAJOR CARPS, POND WATER AND POND SEDIMENT

2021 ◽  
pp. e310
Author(s):  
Avishek Bardhan ◽  
T Jawahar Abraham Thangapalam ◽  
Qurratul Ain Qureshi ◽  
Ratul Chakraborty
Keyword(s):  
West Bengal ◽  
Antibiotic Sensitivity ◽  
Pond Water ◽  
Aquatic Environments ◽  
Antibiotic Resistant ◽  
Pond Sediment ◽  
Indian Major Carps ◽  
High Prevalence ◽  
Resistant Strains ◽  
Major Carps

The uproar in antimicrobial resistance (AMR) among the aquacultured environment has led to the isolation of multiple antibiotic-resistant (MAR) Aeromonas strains. The current study aimed at the enumeration of antibiotic-resistant Aeromonas in carps of aquacultured environment and market samples. Isolation of Aeromonas was also done in Rimler-Shotts agar supplemented with novobiocin followed by antibiotic-sensitivity assay against 12 broad-spectrum antibiotics. Five oxytetracycline-resistant strains were examined for the presence of three tetracycline-resistant genes (tetA, tetC and tetE). The presumptive Aeromonas counts on starch-ampicillin agar were determined as log 3.00-log 6.45/g in carps, log 3.00l-log 5.06/ml in pond water and log 3.30–log 5.14/g in pond sediment. Higher proportions of motile aeromonads from market carps were resistant to chloramphenicol, cefalexin, gentamycin, oxytetracycline and trimethoprim than the farmed carps. Aeromonas strains depicted 57 resistant profiles. About 88.43% of the Aeromonas strains were of the MAR group among which 12.15% and 4.67% were resistant to ≥6 and ≥7 antibiotic groups, respectively. Selected oxytetracycline-resistant strains were negative for targeted genes. The current study implied the high prevalence of AMR bacteria in cultured carps in West Bengal, India. Furthermore, the study indicated that motile aeromonads comprise an effective marker for monitoring AMR in freshwater aquatic environments.

scholarly journals Prevalence of Ectoparasitic Infestation in Indian Major Carps During Winter at Different Blocks of South 24-Parganas District, West Bengal, India

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Koel Bhattacharya Sanyal
Keyword(s):  
Management Practices ◽  
West Bengal ◽  
Monsoon Season ◽  
Quality Parameters ◽  
Catla Catla ◽  
Key Factors ◽  
Post Monsoon ◽  
Indian Major Carps ◽  
Post Monsoon Season ◽  
Major Carps

The study was carried out to find the prevalence of ectoparasites in carp species specifically Indian Major Carps (IMC) during the post monsoon season (November’16 to March’17). Four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from approximately 500 number of  carp species like, Labeo rohita, Catla catla, Cirrhinus mrigala and Labeo calbasu collected from different ponds of selected blocks of South 24 Parganas district of West Bengal. The highest prevalence (64.76%) of infestation had been recorded by Myxozoans and the lowest was by Monogeneans (4.79%). The highest and lowest ectoparasitic prevalence in carp was observed in L. rohita (32.88%) and C. catla (27.28%). At the end of this experiment it was concluded that sudden changes in water quality parameters, over stocking, lack of management practices and some other aspects were the key factors for disease occurrences in this post monsoon season.

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

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