scholarly journals Assessing the antiumer and anti-inflammatory potentials of Calotropis procera latex

2021 ◽  
Vol 4 (1) ◽  
pp. 086-091
Author(s):  
Ruqaya Mohammed Al-ezzy ◽  
Asmaa Sabah Ahmaed ◽  
Nidhal Mohammed Saleh ◽  
Shaymaa Hameed Al-Obaidy
Keyword(s):  
Medicinal Plants ◽  
Cell Line ◽  
Cancer Cell ◽  
Common Factor ◽  
Cancer Cell Line ◽  
Negative Control ◽  
Calotropis Procera ◽  
Cervical Cancer Cell Line ◽  
The Family ◽  
The Indian Ocean

Since time immemorial medicinal plants have been used in healthcare to treat different human disease. The ideals of medicinal plants are highlighted due to the utility of the common-factor approach to engage other health promoters in propagating. One of these medicinal plants is Calotropis procera which is a species of tree in the family Apocynaceae. Calotropis procera is widespread in tropical Africa, including the Indian Ocean islands and the northern parts of South Africa. The latex is toxic and can cause rash, blisters and serious inflammations in sensitive persons and it may lead to blindness. Several side effects may be caused due to ingestion large doses of latex like burning in the throat, irritation of the stomach, nausea, vomiting, diarrhea, tremors, vertigo and convulsions. This study aimed to evaluate burns healing effects and inhibitory concentration IC50 of Calotropis procera latex on cervical cancer cell line, SiHa. Cytotoxicity analysis was performed by MTT assay in addition to determine the burns healing effect of the latex by determining the day requiring to heal the burn skin of albino male mice. The results of burns healing effects declared that the burns required 12 days to heal in comparison with positive (sliver sulfadiazine) and negative control which required 16 and 18 days for healing respectively. Also, the results revealed that the IC50 of latex was 146.8 % in comparison of ambiguous percentage of normal cell line WRL68 with reduction in cancer cell viability ranging from 95.87± 0.20to 52.35 ±3.31 for 6.2 to 400 µg\ml respectively.

Synthesis, Cytotoxicity and Antioxidant Activity of New Analogs of RC-121 Synthetic Derivatives of Somatostatin

2019 ◽  
Vol 18 (10) ◽  
pp. 1417-1424 ◽  
Author(s):  
Emilia Naydenova ◽  
Diana Wesselinova ◽  
Svetlana Staykova ◽  
Ivan Goshev ◽  
Ljubomir Vezenkov
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Synthetic Derivatives ◽  
Derivatives Of

Background: Based on the structure of RC-121 (D-Phe-c (Cys-Tyr-D-Trp-Lys-Val-Cys)-Thr-NH2, - synthetic derivatives of somatostatin), some analogs were synthesized and tested for in vitro cytotoxic and antioxidant activity. Objectives: The new analogs were modifyed at position 5 with Dap (diaminopropanoic acid), Dab (diaminobutanoic acid) and Orn and at position 6 with the unnatural amino acids Tle (t-leucine). Methods: The in vitro cytotoxic effects of the substances were investigated against a panel of human tumor cell lines HT-29 (Human Colorectal Cancer Cell Line), MDA-MB-23 (Human Breast Cancer Cell Line), Hep G-2 (Human Hepatocellular Carcinoma Cell Line) and HeLa (cervical cancer cell line). The antioxidant capacities were tested by ORAC (Oxygen Radical Antioxidant Capacity) and HORAC (Hydroxyl Radical Averting Capacity) methods. Results: All substances expressed significantly higher antioxidant capacity by comparison with galic acid and Trolox. All substances showed considerable antioxidant capacity as well. Compound 2T (D-Phe-c(Cys-Tyr-DTrp- Dap-Tle-Cys)-Thr-NH2)had the highest antioxidant effect. The compound 4T (D-Phe-c(Cys-Tyr-D-Trp- Orn-Tle-Cys)-Thr-NH2) displayed antiproliferative effect on HeLa cells with IC50 30 µM. The peptide analog 3T (D-Phe-c(Cys-Tyr-D-Trp-Lys-Tle-Cys)-Thr-NH2) exerted the most pronounced inhibition on the cell vitality up to 53%, 56% and 65% resp. against MDA-MB-23, Hep G-2, HeLa in the higher tested concentration. Conclusion: The somatostatin analogs showed moderate influence on the vitality of different tumor cells and could be used in changing their pathology.

scholarly journals Retraction: Tetraarsenic Oxide-mediated Apoptosis in a Cervical Cancer Cell Line, SiHa

2007 ◽  
Vol 39 (1) ◽  
pp. 48
Author(s):  
Jeong Kim ◽  
Su-Mi Bae ◽  
Dae-Seog Lim ◽  
Sun-Young Kwak ◽  
Chang-Ki Lee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line

Benzylisoquinoline alkaloids from Nelumbo nucifera Gaertn. petals with antiausterity activities against the HeLa human cervical cancer cell line

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Juthamart Maneenet ◽  
Ashraf M. Omar ◽  
Sijia Sun ◽  
Min Jo Kim ◽  
Supawadee Daodee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Nelumbo Nucifera ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Benzylisoquinoline Alkaloids ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Abstract Ethanolic extract of Nelumbo nucifera petals showed preferential cytotoxic activity against HeLa human cervical cancer cell line with a PC50 value of 10.4 μg/mL. This active extract was subjected to a phytochemical investigation study which led to the isolation of nine benzylisoquinoline alkaloids (1–9). The isolated compounds exhibited potent antiausterity activities. Moreover, under nutrient-deprived conditions, (−)-lirinidine (8) induced remarkable alterations in HeLa cell morphology including cell shrinkage and plasma blebbing leading to total cell death within 10 h. Mechanistically, 8 was found to inhibit Akt/mTOR signaling pathway. It also induced apoptosis by promoting caspase-3 activation and inhibiting Bcl-2 expression. Therefore, benzylisoquinoline alkaloids skeleton can be considered as a promising scaffold for the anticancer drug development against cervical cancer.

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