AKURASI KARAKTERISASI TINGKAT IN SITU TANAMAN UBI JALAR PADA DUA KECAMATAN DI KABUPATEN SERAM BAGIAN BARAT

2017 ◽  
Vol 13 (2) ◽  
pp. 103-110
Author(s):  
Helen Hetharie ◽  
Simon H T Raharjo ◽  
Gelora H Augustyn ◽  
Marietje Pesireron

Morphological characters of above ground plant parts can be used to distinguishsweet potato accessions. The objective of this study was to get a number of diversity of sweet potato accessions based on above ground part morphology and to determine the accuracy of in situ characterization. This study used a survey method in five villages in two sub-districts, namely Inomosol and Huamual Muka, Western Ceram District. The first stage of this study involved in situ characterization, and the second stage involved planting and ex situ morphological characterization of 2-month-old plants which was used sweet potato descriptors. Data were analyzed descriptively and qualitatively. The results showed that there were 25 accessions of sweet potato found in Huamual Muka and Inomosol Sub-districts, as distinguished by leaf shape and color, leaf patterns and leaf lobe number, petiole and stem colors, and vine twisting tendency. Verification results of the above ground morphology showed accuracy of ≥80% in 3 phenotypes in leaf lobe pattern, shape of central leaf lobe, linear middle leaves, leaves with 1, 5 and 7 lobes, green abaxial leaf veins, 2 petiole color phenotypes, purplish red stem, and stem additional colors. Meanwhile, the accuracy of characterization of other phenotypes was 0-76%. Keywords: diversity, ex situ, Ipomoea batatas, Maluku, morphology   ABSTRAK Karakter morfologi tajuk ubi jalar dapat digunakan sebagai pembeda antar aksesi. Tujuan penelitian untuk mendapatkan sejumlah klon ubi jalar yang beragam berdasarkan morfologi tajuk serta ketepatan karakterisasi morfologi pada tingkat in situ. Penelitian ini menggunakan metode survei di lima desa pada dua kecamatan yaitu kecamatan Inomosol dan Huamual Muka pada Kabupaten Seram Bagian Barat, Propinsi Maluku. Tahap pertama dengan mengkarakterisasi morfologi tajuk di kebun petani (in situ), dan tahap kedua penanaman dan karakterisasi ex situ di kebun koleksi pada umur tanaman 2 bulan menggunakan deskriptor ubi jalar. Data dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa terdapat 25 aksesi ubi jalar di kecamatan Inomosol dan Huamual Muka yang dibedakan berdasarkan bentuk dan warna daun, pola dan jumlah lekuk daun, warna tangkai dan batang, serta sifat membelit. Hasil verifikasi terhadap karakterisasi in situ didapatkan akurasi ≥80% pada 3 fenotip pada pola lekuk daun, daun dengan bentuk bagian tengah linear, daun dengan jumlah cuping 1, 5 dan 7, tulang daun permukaan bawah berwarna hijau, 2 fenotip pada warna tangkai daun, batang berwarna merah keunguan, serta ada warna tambahan pada batang. Sedangkan akurasi karakterisasi pada fenotip lainnya yaitu 0-76%. Kata kunci: ex situ, Ipomoea batatas, keragaman, Maluku, morfologi

2021 ◽  
Vol 12 (2) ◽  
pp. 110
Author(s):  
Febri Yuda Kurniawan ◽  
Anindita Della Rosa Riyadi

Peristylus goodyeroides is a terestrial orchid that scattered around Southeast Asia. Morphological characters of P. goodyeroides can vary, depending on the ecological factors and habitat. Cytological characters in the form of chromosome configurations can be used as a taxonomic tool for the process of identifying and understanding variations in taxa. The purpose of this study was to determine the morphological characters and chromosome configuration of the P. goodyeroides from Curug Setawing, Kulonprogo. The method used was morphological characterization and plant chromosome preparation by squash method with the main steps of fixation, maceration, staining and observation. Data were analyzed with the help of Image raster 3, Corel Draw X7, and Microsoft Excel 2013. P. goodyeroides from Curug Setawing has the morphological characters of root tubers, cylindrical stems, ovate leaf shape, convolutive leaf arrangement and creamy white flowers. The orchid has a number of chromosomes 2n = 10 with a karyotype formula of 2n = 2x = 8m + 2t. Metacentric chromosomes are found on chromosomes 1-8 and telocentric chromosomes 9-10. The absolute arm length of the chromosomes has a range of 2.03-3.44 μm, the relative arm length of the chromosomes is 2.21-3.32 μm, the length of the p arm is 1.13-1.58 μm and the q arm is 1.23-2.12 μm.


Scanning ◽  
2014 ◽  
Vol 36 (6) ◽  
pp. 590-598 ◽  
Author(s):  
Quan Shen ◽  
Matthias Edler ◽  
Thomas Griesser ◽  
Astrid‐Caroline Knall ◽  
Gregor Trimmel ◽  
...  

2019 ◽  
Vol 25 (2) ◽  
pp. 15
Author(s):  
Sumilah Sumilah ◽  
Nirmala F. Devy ◽  
Hardiyanto Hardiyanto

<p>The diversity of sweet potatoes from Agam and Solok District of West Sumatra is known to be very high, it causes somedifficulties in taxonomic research. The diversity analysis based on the leaf and flower morphology has not done before in local sweet potato from Agam and Solok District. The purpose of this study was to determine the diversity of local varieties of sweet potato from West Sumatra based on leaves and flowers morphological characters. The analysis was conducted on 21 accessions collected from West Sumatra regencies and germplasm collection. The Morphological characterization was done based on CIP/AVRDC/IBPGR descriptor list. The data of morphological character was analyzed using Primary Component Analysis (PCA) and grouping analysis using Cluster Analysis through SAHN method on NTSys program, with UPGMA as similarities criteria. The results showed that the first four principal components contributed 89.6% to the plant diversity. The sweet potatoes were divided into 2 clusters, with the main distinguishing character was the existence of plant flowers. Information on genetic diversity and the pattern of relations between accessions will be very useful for the West Sumatra people in developing superior sweet potatoes.</p>


2021 ◽  
Vol 4 (1) ◽  
pp. 55-64
Author(s):  
Intan Sari ◽  
Henri Henri ◽  
Eka Sari ◽  
Suharyanto Suharyanto

Ginseng plant (Phytolacca octandra L.) is a wild plant that has the potential to be used as medicine. The morphological characteristics of ginseng plants in Indonesia are different. Therefore, it is necessary to carry out research aimed at characterizing the morphology and phytochemical screening of ginseng (P. octandra L.) leaves and tubers. The research was conducted first by observing morphological characters and screening phytochemical compounds such as alkaloid test, flavonoid test, phenol test, tannin test, saponin test, terpenoid test, and steroid test. The results of the research on the morphological characterization of ginseng (P. octandra L.) were: having a taproot which was modified into a tuber, a round, smooth, red color, pointed leaf shape, the base and tip of the leaf were pointed, buni fruit type, round flat, colored blackish-purple when ripe, a compound flower is located at the end of the branch and has flat, round seeds. Ginseng tubers have triterpenoid and saponin compounds, while the leaves only have saponin compounds. The potential development of ginseng (P. octandra L.) is currently limited as traditional medicine by local people. This is due to limited knowledge in its development efforts and unknown phytochemical content.


1998 ◽  
Vol 512 ◽  
Author(s):  
A. D. Serra ◽  
H. H. Richardson

ABSTRACTFilms of AIN were grown on Si under vacuum pressure at 900°C and examined ex situ with infrared reflectance spectroscopy and microscopy, scanning electron microscopy, x-ray diffraction and rutherford backscattering spectroscopy. Collection of IR emission spectra for in situ characterization was successful and used to identify growth parameters during film growth.


2017 ◽  
Vol 106 (8) ◽  
pp. 2163-2167 ◽  
Author(s):  
Lavinia M. Lewis ◽  
Jianxin Guo ◽  
Ekaterina Torres ◽  
Jijun Wang ◽  
Hilbert Billones ◽  
...  

2009 ◽  
Vol 44 (7) ◽  
pp. 719-725 ◽  
Author(s):  
Vanessa Cavalcante de Almeida ◽  
Lúcia Vieira Hoffmann ◽  
Gilberto Ken It Yokomizo ◽  
Joaquim Nunes da Costa ◽  
Marc Giband ◽  
...  

The objective of this work was to characterize the populations of Gossypium barbadense in the states of Amapá and Pará, Brazil. In situ characterization was conducted through interviews with the owners of the plants and environmental observations. Leaf or petal tissue as well as seed samples were collected for genetic characterization by single sequence repeats markers and for storage in germplasm banks, respectively. The plants were maintained in dooryards and used mainly for medical purposes. The genetic analysis showed no heterozygous plants at the loci tested (f = 1), indicating that reproduction occurs mainly through selfing. The total genetic diversity was high (He = 0.39); and a high level of differentiation was observed between cotton plants from the two states (F ST = 0.36). Conventional methods of in situ maintenance of G. barbadense populations are not applicable. The conservation of the genetic variability of populations present in the two states could be achieved through germplasm collection and establishing of ex situ seed banks.


2013 ◽  
Vol 18 (2) ◽  
pp. 309-323 ◽  
Author(s):  
Hong-Pyo Kim ◽  
Dong-Jin Kim ◽  
Sung-Woo Kim ◽  
Yun-Soo Lim ◽  
Seong-Sik Hwang

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