scholarly journals Direct Smear Versus Liquid-Based Cytology in the Diagnosis of Bladder Lesions

2022 ◽  
Vol 17 (1) ◽  
pp. 56-64
Mahmoud Reza Kalantari ◽  
Mohammad Ali Jahanshahi ◽  
Masoumeh Gharib ◽  
Sara Hashemi ◽  
Shakiba Kalantari
2014 ◽  
Vol 53 (2) ◽  
pp. 89-93 ◽  
Tomomi ASHIKAWA ◽  
Takashi UMEZAWA ◽  
Kouichi NOMURA ◽  
Takekazu ONDA ◽  
Naoki KAMIYA ◽  

2021 ◽  
Vol 9 (8) ◽  
pp. 1575
Kaori Okayama ◽  
Toshiyuki Sasagawa ◽  
Koji Teruya ◽  
Mizue Oda ◽  
Masahiko Fujii ◽  

Many genotypes of human papillomaviruses (HPVs) may lead to morphological changes in cells, resulting in various atypical cells, such as multinucleated cells (MNCs) and koilocytes, in the cervix. However, the relationships between the profiles of HPV genotypes and MNCs are not exactly known. Thus, this study comprehensively profiles the HPV genotypes in MNCs using a microdissection method. HPV genotypes and MNCs were detected in 651 cases with an abnormal Pap smear by liquid-based cytology. Specific HPV genotypes were also detected, including HPV16, 34, and 56, which might be associated with MNCs. This result suggests that the high-risk HPV genotypes, such as HPV16 and 56, are associated with the atypical changes in MNC morphology from normal cervical cells. The results also show that MNCs may be a predictor of squamous intraepithelial lesion.

Parasitology ◽  
2019 ◽  
Vol 146 (12) ◽  
pp. 1602-1614 ◽  
Hesham M. Al-Mekhlafi ◽  
Nabil A. Nasr ◽  
Yvonne A. L. Lim ◽  
Fatin Nur Elyana ◽  
Hany Sady ◽  

AbstractThis cross-sectional study aimed to determine the prevalence and risk factors of S. stercoralis infection among 1142 Orang Asli primary schoolchildren in six different states of Peninsular Malaysia. Fecal samples were examined using direct smear, formalin-ether sedimentation (FES), agar plate culture (APC) and PCR techniques. Overall, 15.8% of the children were found to be infected with S. stercoralis. The prevalence was 0.2, 1.3, 15.2 and 13.7% by direct smear, FES, APC and PCR, respectively. Multivariate analysis showed that an age of >10 years, being male, belonging to a Proto-Malay tribe, belonging to the Senoi tribe, indiscriminate defecation, using an unimproved water source for drinking water and not wearing shoes when outside were the significant risk factors of infection among these children. In conclusion, we provide new evidence on the occurrence of S. stercoralis in Malaysia to show that there is a relatively high prevalence of infection among Orang Asli schoolchildren. Therefore, the use of specific methods for detecting S. stercoralis should be considered when screening these children for intestinal parasites. Moreover, prevention and control measures specific to S. stercoralis should be integrated into the intestinal parasitic infections control programme in Malaysia.

2021 ◽  
pp. 1-6
Satoshi Morito ◽  
Takao Nitanda ◽  
Ryuko Tsukamoto ◽  
Shingo Kamoshida ◽  
Hiroshi Yasui ◽  

Immunocytochemistry (ICC) is an important ancillary technique in clinical cytology for not only identifying and characterizing tumor cells but also gaining prognostic or therapeutic information. Although cell blocks are often prepared for immunocytochemical evaluation of body cavity fluid and fine-needle aspiration specimens, they are not suitable for hypocellular samples. Liquid-based cytology can help prepare additional smears from residual cytological specimens. However, since conventional methods are used for nongynecological specimens in most laboratories, ICC is often limited by the number of cytological smears. Cell transfer methods permit to evaluate several immunocytochemical markers in a single cytological smear. Yet, these methods have some limitations; for example, they are time-consuming (about 3–40 h) and medium membranes with their attached cells are occasionally stretched or torn when peeled off the slides. Therefore, in an attempt to solve these problems, we developed a rapid and reliable cell transfer method using a nylon mesh. Our method requires no special equipment or reagent and can significantly reduce the turnaround time, as compared to previous methods.

Cytopathology ◽  
2006 ◽  
Vol 17 (2) ◽  
pp. 86-93 ◽  
V. A. F. Alves ◽  
A. Castelo ◽  
A. L. Filho ◽  
M. R. Vianna ◽  
E. Taromaru ◽  

2014 ◽  
Vol 58 (6) ◽  
pp. 522-532 ◽  
Shuyue Ren ◽  
Charalambos Solomides ◽  
Rossitza Draganova-Tacheva ◽  
Marluce Bibbo

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