Effects of the ATP-dependent K ( )-channel effectors pinacidil and glibenclamide on liver tissue in an experimental model of epilepsy: A histopathological study

Objective: This research aims to evaluate the histopathological changes after pretreatment with resatorvid against methotrexate induced-liver injury.Methods: 28 male albino-wistar rats divided into random 4 groups (7 rats in each). Control group: Rats left untreated. Vehicle pre-treated group: Rats were administered dimethyl sulfoxide (DMSO) followed by methotrexate (MTX). Methotrexate treated group: Rats left untreated then administered MTX. Resatorvid pre-treated group: Rats were administered resatorvid followed by MTX. 24 h after the end of treatment, the animals were sacrificed. Liver tissue samples dissected out immediately and fixed in 10% formalin. The traditional procedures (paraffin-embedded method) was used to prepare liver tissue for microscopic evaluation by none alcoholic fatty liver disease (NAFLD) Activity Score Components.Results: Liver tissue sections of MTX-treated group show moderate-to-severe steatosis of hepatic cells and micro- and macro- hepatocellular fatty degeneration and giant fatty cysts with chronic inflammatory cells infiltration. While liver tissue sections of the resatorvid pre-treated group show moderate hepatic cellular fatty degeneration, with a decreased number of fatty cysts chains and the inflammation disappeared.Conclusion: Resatorvid hepatoprotective effect against MTX-induced injury was promising throughout resolving the accompanying inflammation and partial restoring histopathological fatty alterations.

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Long-term cultures of human fetal liver cells: a three-dimensional experimental model for monitoring liver tissue development

Journal of Hepatology ◽

10.1016/s0168-8278(98)80323-1 ◽

1998 ◽

Vol 28 (3) ◽

pp. 480-490 ◽

Cited By ~ 3

Author(s):

Massimo Sargiacomo ◽

Paolo Onori ◽

Elena Bravo ◽

Antonio Franchitto ◽

Alfredo Cantafora ◽

...

Keyword(s):

Experimental Model ◽

Liver Tissue ◽

Fetal Liver ◽

Three Dimensional ◽

Liver Cells ◽

Tissue Development ◽

Human Fetal Liver ◽

Fetal Liver Cells ◽

Human Fetal Liver Cells

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A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic ^|^beta;-cells

Endocrine Journal ◽

10.1507/endocrj.ej12-0388 ◽

2013 ◽

Vol 60 (5) ◽

pp. 599-607 ◽

Cited By ~ 8

Author(s):

Masahiro Takei ◽

Katsuya Dezaki ◽

Hiroaki Ishii ◽

Shin-ichi Nishio ◽

Yoshihiko Sato ◽

...

Keyword(s):

Experimental Model ◽

Beta Cells ◽

Insulin Release ◽

Pancreatic Beta Cells ◽

K Channel ◽

Permissive Role

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Tobacco experimental model to induce urinary bladder neoplasms

Revista do Colégio Brasileiro de Cirurgiões ◽

10.1590/s0100-69912014000100011 ◽

2014 ◽

Vol 41 (1) ◽

pp. 056-060 ◽

Cited By ~ 2

Author(s):

José Alexandre Colli Neto ◽

José Hélio Zen Júnior ◽

André Del Negro ◽

Nelson Adami Andreollo ◽

Marina Rachel Araujo ◽

...

Keyword(s):

Experimental Model ◽

Bladder Wall ◽

Bladder Neoplasms ◽

Urinary Bladder Neoplasms ◽

Experimental Models ◽

Histopathological Study ◽

High Concentrations ◽

Carbon Dioxide Co2 ◽

Harmful Effects ◽

Chronic Use

OBJECTIVE: to develop an experimental model of exposure to tobacco burning (cigarette) products to assess the effects of its chronic use in relation to cancers of the bladder. METHODS: the animals were chronically exposed to the burning tobacco products in a semi-open chamber to simulate smoking. Thirty young Wistar rats were divided into two groups: one with 20 animals simulating smoking for six months, and ten not exposed control animals for the same period. After exposure by inhalation of cigarette smoke, animals were euthanized and subjected to histopathological study of the bladder wall. RESULTS: no tumor was found but mild and non significant alterations. The studies of hemo-oximetry (carboxyhemoglobin and methemoglobin) and the concentration of carbon dioxide (CO2) confirm that the animals were exposed to high concentrations of tobacco smoke and its derivatives. CONCLUSION: no bladder mucosal neoplasia was found in the pathological study of animals. The developed experimental models were highly efficient, practical and easy to use and can be used in other similar studies to determine the harmful effects caused by smoking.

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Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl4-Induced Liver Damage

Foods ◽

10.3390/foods9060804 ◽

2020 ◽

Vol 9 (6) ◽

pp. 804

Author(s):

Amani Taamalli ◽

Anouar Feriani ◽

Jesús Lozano-Sanchez ◽

Lakhdar Ghazouani ◽

Afoua El Mufti ◽

...

Keyword(s):

Carbon Dioxide ◽

Liver Tissue ◽

Biochemical Parameters ◽

Research Work ◽

Albino Rats ◽

Histopathological Study ◽

Olive Leaf ◽

Olive Leaves ◽

Leaf Extracts ◽

Ccl4 Treatment

Virgin olive oil has demonstrated its effective activity against oxidative stress. However, data on the bioactive effect of olive leaves or their major constituents on the liver are scarce. The present research work was conducted to evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO2) extracts from fresh and dried olive leaves on hepatotoxicity caused by carbon tetrachloride (CCl4) in rat models. For this purpose, healthy albino rats of 180–250 g weight were used. The assessment of biochemical markers was carried out on blood and liver tissue. Then, a histopathological study was carried out on liver tissue. The obtained results showed that fresh and dried olive leaf extracts ameliorate the perturbed biochemical parameters caused by CCl4 treatment. Furthermore, the results registered for the histopathological study are in accordance with the biochemical parameters and the protective capacity of SC-CO2 extracts against DNA damage, indicating that olive leaf extracts helped to improve liver fibrosis caused by CCl4 treatment.

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