scholarly journals Evaluation of Centromer H Protein (CENPH) Gene Expression in Prostate Cancers

2021 ◽  
Vol 2 (37) ◽  
pp. 151-157
Author(s):  
Ilknur Karalezli ◽  
Ayse Gul Zamani ◽  
Yunus Emre Goger ◽  
Huseyin Osman Yilmaz ◽  
Giray Karalezli
Keyword(s):  
Gene Expression ◽  
Prostate Cancers ◽  
H Protein

HIGH INCIDENCE OF RECEPTORS FOR LUTEINIZING HORMONE-RELEASING HORMONE (LHRH) AND LHRH RECEPTOR GENE EXPRESSION IN HUMAN PROSTATE CANCERS

2000 ◽  
Vol 163 (2) ◽  
pp. 623-629 ◽  
Author(s):  
GABOR HALMOS ◽  
JOSÉ M. ARENCIBIA ◽  
ANDREW V. SCHALLY ◽  
RODNEY DAVIS ◽  
DAVID G. BOSTWICK
Keyword(s):  
Gene Expression ◽  
Luteinizing Hormone ◽  
Receptor Gene ◽  
Human Prostate ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
High Incidence ◽  
Prostate Cancers ◽  
Receptor Gene Expression ◽  
Lhrh Receptor

scholarly journals Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Ying Tan ◽  
Alfredas Bukys ◽  
Attila Molnár ◽  
Andrew Hudson
Keyword(s):  
Gene Expression ◽  
Gene Silencing ◽  
High Efficiency ◽  
Tobacco Rattle Virus ◽  
Transient Gene Expression ◽  
Mutant Phenotype ◽  
Homologous Sequence ◽  
Homologous Gene ◽  
Loss Of Function ◽  
H Protein

Abstract Background Antirrhinum (snapdragon) species are models for genetic and evolutionary research but recalcitrant to genetic transformation, limiting use of transgenic methods for functional genomics. Transient gene expression from viral vectors and virus-induced gene silencing (VIGS) offer transformation-free alternatives. Here we investigate the utility of Tobacco rattle virus (TRV) for homologous gene expression in Antirrhinum and VIGS in Antirrhinum and its relative Misopates. Results A. majus proved highly susceptible to systemic TRV infection. TRV carrying part of the Phytoene Desaturase (PDS) gene triggered efficient PDS silencing, visible as tissue bleaching, providing a reporter for the extent and location of VIGS. VIGS was initiated most frequently in young seedlings, persisted into inflorescences and flowers and was not significantly affected by the orientation of the homologous sequence within the TRV genome. Its utility was further demonstrated by reducing expression of two developmental regulators that act either in the protoderm of young leaf primordia or in developing flowers. The effects of co-silencing PDS and the trichome-suppressing Hairy (H) gene from the same TRV genome showed that tissue bleaching provides a useful marker for VIGS of a second target gene acting in a different cell layer. The ability of TRV-encoded H protein to complement the h mutant phenotype was also tested. TRV carrying the native H coding sequence with PDS to report infection failed to complement h mutations and triggered VIGS of H in wild-type plants. However, a sequence with 43% synonymous substitutions encoding H protein, was able to complement the h mutant phenotype when expressed without a PDS VIGS reporter. Conclusions We demonstrate an effective method for VIGS in the model genus Antirrhinum and its relative Misopates that works in vegetative and reproductive tissues. We also show that TRV can be used for complementation of a loss-of-function mutation in Antirrhinum. These methods make rapid tests of gene function possible in these species, which are difficult to transform genetically, and opens up the possibility of using additional cell biological and biochemical techniques that depend on transgene expression.

scholarly journals Global Gene Expression Characterization of Circulating Tumor Cells in Metastasic Castration-Resistant Prostate Cancer Patients

2020 ◽  
Vol 9 (7) ◽  
pp. 2066
Author(s):  
Luis León-Mateos ◽  
Alicia Abalo ◽  
Helena Casas ◽  
Urbano Anido ◽  
Óscar Rapado-González ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Global Gene Expression ◽  
Castration Resistant Prostate Cancer ◽  
Castration Resistant ◽  
Viable Solution ◽  
Prostate Cancers ◽  
Expression Characterization

Background: Current therapeutic options in the course of metastatic castration-resistant prostate cancers (mCRPC) reinforce the need for reliable tools to characterize the tumor in a dynamic way. Circulating tumor cells (CTCs) have emerged as a viable solution to the problem, whereby patients with a variety of solid tumors, including PC, often do not have recent tumor tissue available for analysis. The biomarker characterization in CTCs could provide insights into the current state of the disease and an overall picture of the intra-tumor heterogeneity. Methods: in the present study, we applied a global gene expression characterization of the CTC population from mCRPC (n = 9), with the goal to better understand the biology of these cells and identify the relevant molecules favoring this tumor progression. Results: This analysis allowed the identification of 50 genes specifically expressed in CTCs from patients. Six of these markers (HOXB13, QKI, MAOA, MOSPD1, SDK1, and FGD4), were validated in a cohort of 28 mCRPC, showing clinical interest for the management of these patients. Of note, the activity of this CTC signature was related to the regulation of MYC, a gene strongly implicated in the biology of mCRPC. Conclusions: Overall, our results represent new evidence on the great value of CTCs as a non-invasive biopsy to characterize PC.

IgG gene expression and its possible significance in prostate cancers

The Prostate ◽  
2011 ◽  
Vol 72 (6) ◽  
pp. 690-701 ◽  
Author(s):  
Yuxuan Liu ◽  
Zhengshan Chen ◽  
Na Niu ◽  
Qing Chang ◽  
Ruishu Deng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancers

Gene expression analysis of prostate cancers

2002 ◽  
Vol 33 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Jian-Hua Luo ◽  
Yan Ping Yu ◽  
Kathleen Cieply ◽  
Fan Lin ◽  
Petrina Deflavia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Prostate Cancers

cAMP induced modifications of HOX D gene expression in prostate cells allow the identification of a chromosomal area involved in vivo with neuroendocrine differentiation of human advanced prostate cancers

2005 ◽  
Vol 205 (2) ◽  
pp. 202-210 ◽  
Author(s):  
M. Cantile ◽  
A. Kisslinger ◽  
L. Cindolo ◽  
G. Schiavo ◽  
V. D'antò ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neuroendocrine Differentiation ◽  
Prostate Cells ◽  
Prostate Cancers

scholarly journals Epigenetic Regulation of Androgen Receptor Gene Expression in Human Prostate Cancers

2000 ◽  
Vol 80 (12) ◽  
pp. 1789-1796 ◽  
Author(s):  
Tsuyoshi Nakayama ◽  
Masatoshi Watanabe ◽  
Hiroyoshi Suzuki ◽  
Minoru Toyota ◽  
Nobuyuki Sekita ◽  
...  
Keyword(s):  
Gene Expression ◽  
Androgen Receptor ◽  
Epigenetic Regulation ◽  
Receptor Gene ◽  
Androgen Receptor Gene ◽  
Human Prostate ◽  
Prostate Cancers ◽  
Receptor Gene Expression

233: Gene Expression Differences Between Prostate Cancers from Obese and Normal Weight Men

2005 ◽  
Vol 173 (4S) ◽  
pp. 64-64
Author(s):  
Stephen J. Freedland ◽  
Jun Luo ◽  
Angelo M. DeMarzo ◽  
Thomas Dunn ◽  
Helen Fedor ◽  
...  
Keyword(s):  
Gene Expression ◽  
Normal Weight ◽  
Prostate Cancers
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