scholarly journals In vitro evaluation of fungal isolates and nutrient amendments for enhancing apple leaf decay

2003 ◽  
Vol 56 ◽  
pp. 90-94
Author(s):  
K. Tshomo ◽  
M. Walter ◽  
A. Stewart ◽  
M.V. Jaspers
Keyword(s):  
Leaf Litter ◽  
Trametes Versicolor ◽  
Black Spot ◽  
Fruit Trees ◽  
Fruiting Bodies ◽  
Fruit Crops ◽  
Leaf Discs ◽  
Fungal Isolates ◽  
Nutrient Amendments

Primary spread of apple black spot is by ascospores produced from overwintering apple leaf litter To find effective degraders of litter an in vitro trial screened 59 fungal isolates for the ability to colonise sterilised senesced apple leaf discs The isolates of Aureobasidium Alternaria Chaetomium Coniothyrium Epicoccum Fusarium Phoma Trichoderma and Ulocladium spp had originated from the phylloplane or leaf litter of fruit crops The whiterot basidiomycete isolates were from fruiting bodies on native beech poplar and fruit trees The 18 isolates that colonised leaf discs most rapidly were further tested with five nutrient leaf amendments for their biodegradation activity on leaf discs After incubation at 10C for 9 weeks the most effective isolates of Trametes versicolor Phoma Epicoccum and Chaetomium spp caused 54 39 37 and 23 weight reduction respectively while the leaf amendments urea and the mixture of Mycorrcintrade; and Digestertrade; caused 29 and 20 reduction respectively These treatments were selected for evaluation in a field trial

scholarly journals Treatment of overwintering apple leaves to reduce primary inoculum of apple black spot

2003 ◽  
Vol 56 ◽  
pp. 95-99
Author(s):  
K. Tshomo ◽  
I.J. Horner ◽  
M. Walter ◽  
A. Stewart ◽  
M.V. Jaspers
Keyword(s):  
Venturia Inaequalis ◽  
Black Spot ◽  
Field Trials ◽  
Water Control ◽  
Primary Inoculum ◽  
Apple Leaves ◽  
Fungal Isolates ◽  
Glass Slides ◽  
Ascospore Production ◽  
Nutrient Amendments

Field trials were conducted in Hawkes Bay and Lincoln into methods of treating overwintering apple leaves to reduce ascospore production by the apple black spot pathogen Venturia inaequalis The leaf treatments comprised three levels of nutrient amendments (including a water control) and five levels of saprophytic fungal isolates (including a nofungus control) in a factorial design Leaves were left to overwinter on the orchard floor and in spring the V inaequalis ascospores released were trapped on glass slides and counted Ascospore numbers were reduced (Plt;005) by the leaf amendment urea which alone caused 73 reduction but not by the BioStarttrade; product The effect of fungal isolates was not significant (P012) although when combined with the water treatment the isolates Chaetomium Phoma and Epicoccum spp and Trametes versicolor reduced numbers of ascospores by 33 27 15 and 28 respectively compared to the nofungus control When combined with urea the Chaetomium isolate reduced ascospore numbers by 92 and 82 compared to the nil fungus/water control treatments in Hawkes Bay and Lincoln respectively indicating that this treatment has potential for reducing primary inoculum of apple black spot

Peculiarities of the phytosanitary state of pome fruits in industrial horticulture

2020 ◽  
Vol 60 (1) ◽  
pp. 159-168
Author(s):  
V. V. Antonenko ◽  
A. V. Zubkov ◽  
S. N. Kruchina
Keyword(s):  
Mechanism Of Action ◽  
Large Scale ◽  
High Efficiency ◽  
Fruit Trees ◽  
Fruit Crops ◽  
Planting Material ◽  
Relative Safety ◽  
Pome Fruits ◽  
Weed Plants ◽  
Spread Of Infection

Data were obtained on the basis of the results of research carried out on the territory of the educational and experimental farm of the Timiryazev State Agrarian University, in Moscow during 2018-2019. As a result of the surveys, the most dangerous diseases and pests of pome crops on the territory of this farm were established. The most resistant apple and pear varieties to major diseases have been identified. Peculiarities of development of alternariosis on pear are described, the harmfulness of the disease on pear and apple seedlings is noted. A possible role in the transfer of alternariosis infection from garden-protective plantations and weed vegetation to fruit trees was noted. A possible role has been established in the transport of septoriosis, powdery dew infection from dicotyledonous weeds plants. The peculiarities of the spread of infection under the influence of wind direction are noted. The results and peculiarities of the application of various methods of scaring birds in the orchard are presented. As a result of route surveys the most harmful weed plants have been identified. The possibility of using herbicides of different mechanism of action in fruit gardens for weed control has been studied. High efficiency and relative safety of application of herbicides of contact action in nursery fields, operational orchards and for control of piglets on fruit trees are shown. Recommendations are given for the use of soil and systemic herbicides of soil in seedlings beds, the first and second fields of the nursery, as well as in the process of production of large-scale planting material and operational orchards of fruit crops. The safety of the herbicides in question is established when used in accordance with the recommended methods of use.

scholarly journals Using Commercial Compost as Control Measures against Cucumber Root-Rot Disease

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Kamel Kamal Sabet ◽  
Magdy Mohamed Saber ◽  
Mohamed Adel-Aziz El-Naggar ◽  
Nehal Samy El-Mougy ◽  
Hatem Mohamed El-Deeb ◽  
...  
Keyword(s):  
Root Rot ◽  
Control Measure ◽  
Disease Incidence ◽  
Pathogenic Fungi ◽  
Pythium Ultimum ◽  
Control Measures ◽  
Infested Soil ◽  
Fungal Isolates ◽  
Cucumber Root

Five commercial composts were evaluated to suppress the root-rot pathogens (Fusarium solani (Mart.) App. and Wr, Pythium ultimum Trow, Rhizoctonia solani Kuhn, and Sclerotium rolfsii Sacc.) of cucumber plants under in vitro and greenhouse conditions. In vitro tests showed that all tested unautoclaved and unfiltrated composts water extracts (CWEs) had inhibitor effect against pathogenic fungi, compared to autoclaved and filtrated ones. Also, the inhibitor effects of 40 bacteria and 15 fungi isolated from composts were tested against the mycelial growth of cucumber root-rot pathogens. Twenty two bacteria and twelve fungal isolates had antagonistic effect against root-rot pathogens. The antagonistic fungal isolates were identified as 6 isolates belong to the genus Aspergillus spp., 5 isolates belong to the genus Penicillium spp. and one isolate belong to the genus Chaetomium spp. Under greenhouse conditions, the obtained results in pot experiment using artificial infested soil with cucumber root-rot pathogens showed that the compost amended soil reduced the percentage of disease incidence, pathogenic fungi population, and improved the cucumber vegetative parameters as shoot length, root length, fresh weight, and dry weight. These results suggested that composts are consequently considered as control measure against cucumber root-rot pathogens.

Chemical characterization and in vitro fermentation ofBrassicastraw treated with the aerobic fungus,Trametes versicolor

2011 ◽  
Vol 91 (4) ◽  
pp. 695-702 ◽  
Author(s):  
J. E. Ramirez-Bribiesca ◽  
Y. Wang ◽  
L. Jin ◽  
T. Canam ◽  
J. R. Town ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Trametes Versicolor ◽  
Lignin Degradation ◽  
Volatile Fatty Acids ◽  
Degradation Products ◽  
Chemical Characterization ◽  
Gas Production ◽  
In Vitro Fermentation ◽  
Lignin Degradation Products

Ramirez-Bribiesca, J. E., Wang, Y., Jin, L., Canam, T., Town, J. R., Tsang, A., Dumonceaux, T. J. and McAllister, T. A. 2011. Chemical characterization and in vitro fermentation of Brassica straw treated with the aerobic fungus, Trametes versicolor . Can. J. Anim. Sci. 91: 695–702. Brassica napus straw (BNS) was either not treated or was treated with two strains of Trametes versicolor; 52J (wild type) or m4D (a cellobiose dehydrogenase-deficient mutant) with four treatments: (i) untreated control (C-BNS), (ii) 52J (B-52J), (iii) m4D (B-m4D) or (iv) m4D+glucose (B-m4Dg). Glucose was provided to encourage growth of the mutant strain. All treatments with T. versicolor decreased (P<0.05) neutral-detergent fibre and increased (P<0.05) protein and the concentration of lignin degradation products in straw. Ergosterol was highest (P<0.05) in straw treated with B-52J, suggesting it generated the most fungal biomass. Insoluble lignin was reduced (P<0.05) in straw treated with B-52J and B-m4D, but not with B-m4Dg. Mannose and xylose concentration were generally higher (P<0.05) in straw treated with fungi, whereas glucose and galactose were lower as compared with C-BNS. The four treatments above were subsequently assessed in rumen in vitro fermentations, along with BNS treated with 2 mL g−1of 5 N NaOH. Concentrations of total volatile fatty acids after 24 and 48h were lower (P<0.05) in incubations that contained BNS treated with T. versicolor as compared with C-BNSor NaOH-treated BNS. Compared with C-BNS, in vitrodry matter disappearance and gas production were increased (P<0.05) by NaOH, but not by treatment with either strain of T. versicolor. Although treatment with T. versicolor did release more lignin degradation products, it did not appear to provide more degradable carbohydrate to in vitro rumen microbial populations, even when a mutant strain with compromised carbohydrate metabolism was utilized. Production of secondary compounds by the aerobic fungi may inhibit rumen microbial fermentation.

IN VITRO SELECTION OF DISEASE RESISTANCE IN FRUIT TREES

1995 ◽  
pp. 19-26 ◽  
Author(s):  
F. Hammerschlag ◽  
D. Ritchie ◽  
D. Werner ◽  
G. Hashmil ◽  
L. Krusberg ◽  
...  
Keyword(s):  
Disease Resistance ◽  
In Vitro Selection ◽  
Fruit Trees ◽  
Selection Of

scholarly journals In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 554
Author(s):  
Rafael J. Mendes ◽  
Laura Regalado ◽  
João P. Luz ◽  
Natália Tassi ◽  
Cátia Teixeira ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Antimicrobial Peptides ◽  
Erwinia Amylovora ◽  
Growth Curves ◽  
Fruit Trees ◽  
Membrane Permeabilization ◽  
Control Measures ◽  
Minimal Bactericidal Concentration ◽  
Pome Fruit

Fire blight is a major pome fruit trees disease that is caused by the quarantine phytopathogenic Erwinia amylovora, leading to major losses, namely, in pear and apple productions. Nevertheless, no effective sustainable control treatments and measures have yet been disclosed. In that regard, antimicrobial peptides (AMPs) have been proposed as an alternative biomolecule against pathogens but some of those AMPs have yet to be tested against E. amylovora. In this study, the potential of five AMPs (RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) together with BP100, were assessed to control E. amylovora. Antibiograms, minimal inhibitory, and bactericidal concentrations (minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), growth and IC50 were determined and membrane permeabilization capacity was evaluated by flow cytometry analysis and colony-forming units (CFUs) plate counting. For the tested AMPs, the higher inhibitory and bactericidal capacity was observed for RW-BP100 and CA-M (5 and 5–8 µM, respectively for both MIC and MBC), whilst for IC50 RW-BP100 presented higher efficiency (2.8 to 3.5 µM). Growth curves for the first concentrations bellow MIC showed that these AMPs delayed E. amylovora growth. Flow cytometry disclosed faster membrane permeabilization for CA-M. These results highlight the potential of RW-BP100 and CA-M AMPs as sustainable control measures against E. amylovora.

Venturia pirina. [Descriptions of Fungi and Bacteria].

1974 ◽  
Author(s):  
A. Sivanesan
Keyword(s):  
Leaf Litter ◽  
Geographical Distribution ◽  
Primary Infection ◽  
Secondary Infection ◽  
Apple Scab ◽  
Black Spot ◽  
Eriobotrya Japonica ◽  
Venturia Pirina ◽  
Young Growth

Abstract A description is provided for Venturia pirina. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Principally on pear (Pyrus communis) and other Pyrus spp., also recorded from Eriobotrya japonica (loquat) (Herb. IMI). DISEASE: Causes scab or black spot of pear, which results in loss of quantity and quality of fruit. The disease attacks shoots, buds, leaves and fruit, symptoms and aetiology being very similar to those of apple scab caused by V. inaequalis on Malus spp. (CMI Descript. 401). Dark, more or less circular scabs are produced on leaves and fruit, often with some growth distortion. Infection of young wood is more common than with apple scab and causes pale brown blister-like lesions which burst to release conidia in the following year. GEOGRAPHICAL DISTRIBUTION: Worldwide in temperate and subtropical regions wherever pears are grown (see CMI Map 367, ed. 2, 1968). TRANSMISSION: Epidemiology is similar to that of apple scab. The overwintering saprophytic perithecial stage on leaf litter releases airborne ascospores in spring which infect young growth, and secondary infection by conidia dispersed during wet summer weather also occurs. Overwintering lesions on young wood are more frequent than with apple scab and conidia produced by these in the spring can be an important source of primary infection (46, 2061; 47, 849).

scholarly journals The Effects of Nutrition and Environmental Factors on Conidial Germination and Appressorium Formation of Phyllosticta citricarpa, the Causal Agent of Citrus Black Spot

2019 ◽  
Vol 109 (4) ◽  
pp. 650-658 ◽  
Author(s):  
Nan-Yi Wang ◽  
Megan M. Dewdney
Keyword(s):  
Black Spot ◽  
Nitrogen Sources ◽  
Conidial Germination ◽  
Appressorium Formation ◽  
Potato Dextrose Broth ◽  
Citrus Black Spot ◽  
Fungal Biology ◽  
Biological Studies ◽  
Phyllosticta Citricarpa

Citrus black spot, caused by Phyllosticta citricarpa, has been identified in Florida since 2010 and can reduce fruit yield and marketability. The conditions required for conidial germination have been poorly understood for P. citricarpa, limiting further biological studies. In this study, the effects of citrus juices, concentration, pH, various carbon and nitrogen sources, and environmental conditions were evaluated in vitro. All tested juices, especially ‘Valencia’ (>85%, P < 0.05), favored germination and appressorium formation, whereas sterile water rarely stimulated germination (<1%). The ‘Valencia’ juice effect was concentration and pH dependent, and the maximum rate was reached in 1.5% juice with pH of 3.4. Most carbon, nitrogen, or complex sources did not favor germination or appressorium formation, with the exception of potato dextrose broth. An incubation period of 18 to 24 h at 24°C was required for peak germination and appressorium formation. The further analysis of critical juice components using synthetic citrus juice revealed that sugars, salts, citric acid, and thiamine were most important for germination and appressorium formation (>80%, P > 0.05). These results provide a better understanding of fungal biology of P. citricarpa and a robust and convenient system for further applications such as screening for efficacious fungicides.

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