scholarly journals Intensity of free-radical processes in plasma of rat blood due to histamine and quercetin

2020 ◽  
pp. 36-52
Author(s):  
N. Harasym ◽  
◽  
M. Verbeschuk ◽  
N. Bodnarchuk ◽  
M. Galan ◽  
...  
Keyword(s):  
Free Radical ◽  
Blood Plasma ◽  
Oxidative Modification ◽  
Combined Effect ◽  
Simultaneous Action ◽  
Carbonyl Groups ◽  
Oxidative Modification Of Proteins ◽  
Rat Blood ◽  
Free Radical Processes ◽  
Radical Processes

The content of products of lipoperoxidation and oxidative modification of proteins in rat blood plasma under the action of quercetin and histamine was investigated. Quercetin was used at concentrations of 0.1; 0.3; 0.5; 1; 3; 5 mm, and histoma - 0.01; 0.1; 1; 10 μm. It was found that quercetin in the blood plasma of rats causes a slight increase in the content of hydroperoxides and TBA-positive products, except the concentration of 0.5 mm, at which the content of secondary products is reduced and the content of primary remains within the control. Histamine at concentrations of 0.01; 0.1; 1; 10 µM leads to an increase in the content of hydroperoxides and a decrease in the number of TBK-positive products. The combined effect of histamine and quercetin causes a significant increase in primary lipoperoxidation products, whereas TBK-positive lipid peroxidation products decrease. Quercetin in the concentration range of 0.1÷1 mM intensifies the accumulation of carbonyl groups of proteins of basic and neutral nature, while flavonoid at concentrations of 3.5 mM – slows down. Histamine at all tested concentrations leads to an increase in the content of carbonyl groups of proteins, except the concentration of 0.1 μm. Against the background of high concentration histamine, quercetin reduces the intensity of oxidative modification of proteins. The simultaneous action of low concentration histamine and quercetin at a concentration of 0.5 and 5 mm leads to the accumulation of carbonyl groups of proteins of a neutral nature, as well as to the main only when exposed to quercetin at a concentration of 5 mm. According to the analysis of variance, it was found that quercetin has a maximum effect on the accumulation of TBA-positive products and carbonyl groups of proteins of basic character. Histamine is strongly influenced by the accumulation of lipid hydroperoxides. Quercetin at concentrations of 0.1; 0.3; 0.5; 1; 3 mm determines between the indices of free radical processes under study, in the blood plasma of rats, a strong interconnectedness, and a bioflavonoid at a concentration of 5 mm leads to the formation of interconnections of average strength. Histamine at concentrations of 10; 1; 0.1 µM causes a close correlation of the mean strength between the individual indices of free radical oxidation. Histamine at a concentration of 0.01 μm causes a significant increase in the correlation between the parameters of sex and oxidative modification of proteins. The combined effect of quercetin at a concentration of 0.1 mm and histamine at a concentration of 0.01 μm causes a change in the nature of the correlation dependence, which becomes strong between most of the studied parameters.

scholarly journals INTENSITY OF FREE RADICAL PROCESSES IN THE POSTNUCLEAR FRACTION OF RAT KIDNEYS UNDER INFLUENCE OF LASER IRRADIATION AND ω-3 POLYUNSATURATED FATTY ACIDS

2021 ◽  
Vol 26 (2(49)) ◽  
pp. 11-22
Author(s):  
O. V. Ketsa ◽  
M. M. Marchenko ◽  
О. А. Samuliak
Keyword(s):  
Free Radical ◽  
Laser Irradiation ◽  
Antioxidant Effect ◽  
The Body ◽  
Oxidative Modification ◽  
Oxidative Modification Of Proteins ◽  
Free Radical Processes ◽  
Low Intensity ◽  
Radical Processes ◽  
Intensity Laser

Introduction. The effect of laser irradiation on the body can lead to local tissue damage, which will be accompanied by changes in biochemical processes that occur in the kidneys. The direction of these changes is completely unclear, so the study of free radical oxidation of lipids and proteins as the main markers of oxidative stress in the body and their correction by ω-3 polyunsaturated fatty acids (PUFA) remains relevant. Aim.  To evaluate the intensity of lipid peroxidation and oxidative modification of proteins in the postnuclear fraction of rat kidneys under the action of laser irradiation and additional administration of ω-3 PUFA. Methods. The studies used white outbred rats, which were irradiated daily for 4 minutes with a laser diode with a wavelength of 650 nm, power of 50 mW in the abdominal cavity at a distance of 10 cm from the skin surface. The state of lipid peroxidation (LPO) was determined by the level of primary, secondary and final products. The intensity of oxidative modification of proteins (OMP) was evaluated by the level of carbonyl derivatives and protein SH-groups. To correct the prooxidant state, the animals were additionally injected with ω-3 PUFA. Results. Low-intensity laser irradiation has a destructive effect on the cell membranes of the kidneys, which is expressed by an increase in primary, secondary and final products of LPO in phospholipid extracts and an increase in the intensity of OMP. The introduction of ω-3 PUFAs reduces free radical processes in the kidneys of irradiated rats, but this effect depends on the scheme of their introduction. ω-3 PUFAs do not show antioxidant effect under the conditions of their introduction after laser irradiation. Daily administration of ω-3 PUFA two hours before irradiation shows a slight antioxidant effect only in the initial stages of irradiation. Preliminary seven-day administration of ω-3 PUFA before laser irradiation is the most effective, as it helps to reduce free radical processes. Conclusion. It is established that ω-3 PUFAs are able to have a corrective effect on the action of low-intensity laser irradiation, which depends on the scheme of their introduction. The highest antioxidant effect is observed in groups of animals to which ω-3 PUFA was previously administered before the action of laser irradiation.

Features of free radical processes in the liver of rats with a nutrient imbalance

2020 ◽  
Vol 66 (5) ◽  
pp. 386-391
Author(s):  
O.N. Voloshchuk ◽  
Yu.V. Stus ◽  
G.P. Kopylchuk
Keyword(s):  
Free Radical ◽  
Critical Role ◽  
Liver Mitochondria ◽  
Protein Supplementation ◽  
Oxidative Modification ◽  
Free Radical Processes ◽  
Nutrient Imbalance ◽  
Low Protein ◽  
Radical Processes ◽  
High Sucrose

The activity of free radical processes in liver mitochondria was investigated in rats kept on high-sucrose and low protein/high-sucrose diets. Excess of dietary sucrose caused intensification of free radical processes in liver mitochondria as evidenced by increased hydroxyl radical generation, accumulation of primary (conjugated dienes, ketodienes) and secondary products (TBA-reactive products) of lipid peroxidation, increased cholesterol/phospholipids ratio and also accumulation of oxidative modification products of proteins (carbonyl derivatives). Additional nutritional protein deficiency (low protein/high-sucrose diet) enhanced destructive changes in liver mitochondria. This suggests a critical role of nutrient protein supplementation for maintaining the functional activity of mitochondria. The established changes can be considered as one of possible mechanisms of functional liver activity violation in conditions of nutrient imbalance.

scholarly journals Regulation of free radical processes in healthy volunteers during experimental hyperthermia and in patients with coronary artery disease during summer heat waves

2021 ◽  
Vol 6 (5) ◽  
pp. 19-29
Author(s):  
A. K. Tikhaze ◽  
G. G. Konovalova ◽  
M. K. Osyaeva ◽  
M. D. Smirnova ◽  
T. V. Martynyuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Blood Plasma ◽  
Heat Waves ◽  
Free Radical Processes ◽  
Oxidative Stress Parameters ◽  
Summer Heat ◽  
Artery Disease ◽  
Radical Processes ◽  
In Blood Plasma

Background. In view of the worsening forecast for global temperature rise worldwide, it seems relevant to study the effects of abnormal heat waves on systemic regulatory processes in people with chronic diseases, in particular coronary artery disease (CAD).Aims. This study aimed to investigate the effect of hyperthermia on oxidative stress parameters in patients with various severity of CAD and in healthy subjects.Materials and methods. We studied the level of malonic dialdehyde (MDA) and the activity of Cu,Zn-containing superoxide dismutase (Cu,Zn-SOD) in healthy subjects under conditions of 30-day long simulated hyperthermia and in patients with different severity of CAD after the summer heat wavesResults. We revealed signs of oxidative stress in healthy volunteers during model hyperthermia that manifested as an increase in content of MDA in blood plasma. At the  same time we observed increasing activity of Cu,Zn-SOD in erythrocytes that  utilizes reactive oxygen species. The increase of Cu,Zn-SOD activity started with a certain latency what also can be explained by de novo enzyme biosynthesis induction. We also studied oxidative stress parameters in patients at high and moderate cardiovascular risk according to the SCORE risk chart with uncomplicated CAD course and in patients with complicated CAD with severe coronary damage according to angiography during the summer heat waves. We observed accumulation of MDA in blood plasma and increasing activity of erythrocyte Cu,Zn-SOD in patients with uncomplicated CAD. At the same time we noted that accumulation of MDA in blood plasma was not followed by any increase in activity of red blood cell Cu,Zn-SOD in patients with severe complicated CAD. This fact indicates dysregulation of free radical processes in patients with severe course of CAD during the heat waves.Conclusions. The dysregulation of free-radical processes in patients with a severe clinical course of CAD has been revealed. 

IntensIty of free radical processes in rats’ blood while deep hypothermIa and self-warmIng

2016 ◽  
Vol 1 (5) ◽  
pp. 104-109
Author(s):  
Исмаилова ◽  
Zhamilya Ismailova ◽  
Кличханов ◽  
Nisred Klichkhanov ◽  
Астаева ◽  
...  
Keyword(s):  
Free Radical ◽  
Body Temperature ◽  
Blood Cell ◽  
Blood Plasma ◽  
Red Blood Cell ◽  
Deep Hypothermia ◽  
The Body ◽  
Oxidative Modification ◽  
Warming Rate ◽  
Radical Processes

Hypothermic conditions of homoisothermic organisms are characterized by the activation of free-radical processes in tissues. The intensity of these processes occurring at hypothermia is less well understood. The essential increase in heart rate, breathing, blood flow velocity, and metabolic processes during warming must stimulate the generation of reactive oxygen species and oxidative modification of biomolecules. We study the levels of peroxidation markers of lipids (by malondialdehyde) and proteins (by carbonyl groups) in blood plasma and erythrocytes as well as the activity of erythrocyte antioxidant enzymes of rats after deep hypothermia (the temperature in the rectum was 20°C) and self-warming dynamics. A maximum warming rate (0.016°С/min) was revealed over the body temperature range of 22–33°С, below and above these temperatures a warming rate was essentially lower. The warming of rats resulted in a total protein content reduction which negatively correlates (r = –0.967; р<0.05) with a middle molecular peptide level. The deep hypothermia decreased the intensity of oxidative modification of lipid and proteins in blood plasma and red blood cell membranes, and the activity of red blood cell superoxide dismutase (SOD). Maximum amount of products of oxidative modification of lipids and proteins in plasma and erythrocytes membranes caused by rats’ self-warming was observed at body temperature of 30–35°С. After a complete rats’ warming the intensity of oxidative modification of lipids and proteins in plasma and erythrocyte membranes decreased. The activity of SOD and catalase of erythrocyte substantially increased when body temperature reached 35°С. The obtained data indicate that during self-warming at the body temperature of 30–35°С the oxidative stress appears in blood which requires the use of antioxidant defense.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

Sign in / Sign up

Export Citation Format

Share Document