Recherche de nouvelles activités saccharifiantes thermostables chez les champignons filamenteux

1990 ◽  
Vol 36 (9) ◽  
pp. 625-630 ◽  
Author(s):  
P. Vanacker ◽  
B. Bacle ◽  
G. Vidal ◽  
L. Lacoste
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Key Words ◽  
Filamentous Fungi ◽  
Half Life ◽  
The Other ◽  
Thermostable Enzymes ◽  
Life Measure ◽  
Industrial Use ◽  
Better Than

We have searched for producers of a saccharifying activity with improved thermostability compared with industrial amyloglucosidases. These producers were chosen among thermophilic, thermotolerant, or even mesophilic fungi. Among the 846 isolated strains, five species (two Thermoascus spp., a member of the Aspergillus fumigatus group, and two members of the Aspergillus niger group) showed an amyloglucosidasic complex with the required property. Whereas the first three of these were thermophilic or thermotolerant strains, the latter two strengthen the idea that mesophilic strains can produce thermostable enzymes. The thermostability of the saccharifying complex of the Thermoascus spp., established with a half-life measure, was found to be far better than the other ones. The industrial use of these strains was discussed, and Thermoascus crustaceus seems to be the most advantageous one. Key words: filamentous fungi, amyloglucosidases, thermostability, Thermoascus.

Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

2010 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
S Utomo ◽  
E Boquifai
Keyword(s):  
Key Words ◽  
The Other ◽  
Effect Of Temperature ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design ◽  
Motility Of Sperm ◽  
Better Than

<p>This research was conducted to investigate the effect of temperature and duration of<br />thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded<br />that thawing of frozen semen will have better done at 37°C with duration of 15 second.</p><p>Key words : Thawing, sperm, ministraw, frozen semen.</p>

Intracellular location of enzymes involved in citrate production by Aspergillus niger

1991 ◽  
Vol 37 (11) ◽  
pp. 823-827 ◽  
Author(s):  
Walter M. Jaklitsch ◽  
Christian P. Kubicek ◽  
Michael C. Scrutton
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Key Words ◽  
Filamentous Fungi ◽  
Isocitrate Dehydrogenase ◽  
Enzyme Activities ◽  
Acid Production ◽  
Pyruvate Carboxylase ◽  
Citric Acid Production ◽  
Intracellular Location

The intracellular distribution and maximal activities of nine enzymes involved in the biosynthesis and degradation of citric acid in Aspergillus niger were determined under conditions of growth and of citric acid production. Under these conditions the intracellular location of the enzymes in most cases resembled that described for other filamentous fungi. Pyruvate carboxylase was found predominantly or exclusively in the cytosol. A single isoenzyme of NADP–isocitrate dehydrogenase was present, which appeared to be localised in the mitochondrion. No significant differences in maximal enzyme activities were observed except for NADP–isocitrate dehydrogenase, which showed decreased activity in production-phase mycelia. The results obtained support the scheme proposed by C. P. Kubicek for the intracellular organisation of citric acid formation but provide little evidence that this process is controlled at the level of the biosynthesis of any of the enzymes examined here. Key words: pyruvate carboxylase, citric acid production, enzyme compartmentation, Aspergillus niger.

scholarly journals Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

2010 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
S Utomo ◽  
E Boquifai
Keyword(s):  
Key Words ◽  
The Other ◽  
Effect Of Temperature ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design ◽  
Motility Of Sperm ◽  
Better Than

<p>This research was conducted to investigate the effect of temperature and duration of<br />thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded<br />that thawing of frozen semen will have better done at 37°C with duration of 15 second.</p><p>Key words : Thawing, sperm, ministraw, frozen semen.</p>

scholarly journals Enhancement of Biodegradation of Palm Oil Mill Effluents by Local Isolated Microorganisms

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mohammadreza Soleimaninanadegani ◽  
Soheila Manshad
Keyword(s):  
Staphylococcus Aureus ◽  
Aspergillus Niger ◽  
Chemical Oxygen Demand ◽  
Aspergillus Fumigatus ◽  
Effective Treatment ◽  
Palm Oil ◽  
Biochemical Oxygen Demand ◽  
Oxygen Demand ◽  
Palm Oil Mill ◽  
Better Than

This study was designed to investigate the microorganisms associated with palm oil mill effluent (POME) in Johor Bahru state, Malaysia. Biodegradation of palm oil mill effluents (POME) was conducted to measure the discarded POME based on physicochemical quality. The bacteria that were isolated are Micrococcus species, Bacillus species, Pseudomonas species, and Staphylococcus aureus, while the fungi that were isolated are Aspergillus niger, Aspergillus fumigatus, Candida species, Fusarium species, Mucor species, and Penicillium species. The autoclaved and unautoclaved raw POME samples were incubated for 7 days and the activities of the microorganisms were observed each 12 hours. The supernatants of the digested POME were investigated for the removal of chemical oxygen demand (COD), color (ADMI), and biochemical oxygen demand (BOD) at the end of each digestion cycle. The results showed that the unautoclaved raw POME sample degraded better than the inoculated POME sample and this suggests that the microorganisms that are indigenous in the POME are more effective than the introduced microorganisms. This result, however, indicates the prospect of isolating indigenous microorganisms in the POME for effective biodegradation of POME. Moreover, the effective treatment of POME yields useful products such as reduction of BOD, COD, and color.

scholarly journals Effect of size material on jute-cotton union fabric

1970 ◽  
Vol 4 (2) ◽  
pp. 36-38
Author(s):  
Md Abul Kalam Azad ◽  
Shamina Jafrin
Keyword(s):  
Key Words ◽  
Cotton Fabric ◽  
The Other ◽  
Weft Yarn ◽  
Cotton Yarn ◽  
Warp Yarn ◽  
Weft Direction ◽  
International University ◽  
Jute Yarn ◽  
Better Than

A study was performed on the jute-cotton union fabric to show the effect of sizing on the properties of union fabric. Here, the cotton yarn was used in the warp direction and jute yarn was used in the weft direction. The size material was used only for warp yarn that is for cotton yarn. From the experimental results, it was seen that the strength of the sized union fabric was better than the unsized union fabric although the other properties were similar to each other. Comparing the properties of union fabric with 100% cotton fabric, union fabric (jute-cotton) may be the replacement of 100% same category of cotton fabric. Key words: Jute, union fabric, cotton fabric, warp yarn, weft yarn, sized, properties. DOI: 10.3329/diujst.v4i2.4363 Daffodil International University Journal of Science and Technology Vol.4(2) 2009 pp.36-38

scholarly journals Study on the effect of size material on jute-cotton union fabric

1970 ◽  
Vol 4 (1) ◽  
pp. 42-44
Author(s):  
Md Abul Kalam Azad ◽  
Shamina Jafrin
Keyword(s):  
Key Words ◽  
Cotton Fabric ◽  
The Other ◽  
Weft Yarn ◽  
Cotton Yarn ◽  
Warp Yarn ◽  
Weft Direction ◽  
International University ◽  
Jute Yarn ◽  
Better Than

A study was performed on the jute-cotton union fabric to show the effect of sizing on the properties of union fabric. Here, the cotton yarn was used in the warp direction and jute yarn was used in the weft direction. The size material was used only for warp yarn that is for cotton yarn. From the experimental results, it was seen that the strength of the sized union fabric was better than the unsized union fabric although the other properties were similar to each other. Comparing the properties of union fabric with 100% cotton fabric, union fabric (jute-cotton) may be the replacement of 100% same category of cotton fabric. Key words: Jute, union fabric, cotton fabric, warp yarn, weft yarn, sized, properties. DOI: 10.3329/diujst.v4i1.4354 Daffodil International University Journal of Science and Technology Vol.4(1) 2009 pp.42-44

Closing the GAP—A 5Å Scanning Microscope

1969 ◽  
Vol 27 ◽  
pp. 6-7
Author(s):  
A. V. Crewe
Keyword(s):  
Normal Form ◽  
The Other ◽  
Resolving Power ◽  
Scanning Microscope ◽  
Conventional Microscope ◽  
Other Hand ◽  
Closing The Gap ◽  
Thermal Sources ◽  
Better Than ◽  
Transmission Microscope

We have become accustomed to differentiating between the scanning microscope and the conventional transmission microscope according to the resolving power which the two instruments offer. The conventional microscope is capable of a point resolution of a few angstroms and line resolutions of periodic objects of about 1Å. On the other hand, the scanning microscope, in its normal form, is not ordinarily capable of a point resolution better than 100Å. Upon examining reasons for the 100Å limitation, it becomes clear that this is based more on tradition than reason, and in particular, it is a condition imposed upon the microscope by adherence to thermal sources of electrons.

Turnover and Distribution of 131Iodine-Labelled Human Fibrinogen

1964 ◽  
Vol 11 (02) ◽  
pp. 404-422 ◽  
Author(s):  
Annemarie Amris ◽  
C. J Amris
Keyword(s):  
Body Weight ◽  
Cancer Patient ◽  
Distribution Ratio ◽  
Half Life ◽  
Fibrinolytic Activity ◽  
The Other ◽  
Turnover Rates ◽  
Human Fibrinogen ◽  
Coagulation Defects ◽  
Fractional Turnover

Summary14 patients (5 diabetics with arteriosclerotic complications, 4 patients with thrombo-embolic disease, 4 with cirrhosis, coagulation defects and increased fibrinolytic activity, and 1 cancer patient) and 3 control patients were subjected to turnover studies with 13iodine labelled human fibrinogen.Half-life times in the control patients were found to be 4 days, the fractional turnover rates 19–23 per cent, of intravascular fibrinogen per day, and the absolute turnover 0.02 to 0.06 gm per day per kg. body weight. The other patient’s half-life times and turnover rates varied considerably from 0.9–5.5 days, 13–160 per cent, per day of intravascular fibrinogen and 0.02–0.4 gm per day per kg. body weight respectively.As fibrinogen unlike other proteins subjected to turnover studies, is converted to fibrin, it is not possible to measure the true intra-extravascular distribution ratio of fibrinogen. But intravascular fibrinogen could be approximated to constitute 68–99 per cent, of the total fibrinogen. There is justification in believing that fibrinogen is degradated through a continuous coagulation in equilibrium with fibrinolysis, and that the organism contains a greater mass of fibrin, the “fibrin pool”. Considerations of the turnover mechanism can however only be hypothetical.

MUNDANE MIRACLES IN AWADH. ENCOUNTERS WITH THE REVEALED AND THE HIDDEN

2020 ◽  
pp. 130-140
Author(s):  
Maxim B. Demchenko ◽  
Keyword(s):  
The Other ◽  
Local People ◽  
Natural Phenomena ◽  
Mughal Empire ◽  
The Public ◽  
British Raj ◽  
The Subject ◽  
Other World ◽  
Better Than

The sphere of the unknown, supernatural and miraculous is one of the most popular subjects for everyday discussions in Ayodhya – the last of the provinces of the Mughal Empire, which entered the British Raj in 1859, and in the distant past – the space of many legendary and mythological events. Mostly they concern encounters with inhabitants of the “other world” – spirits, ghosts, jinns as well as miraculous healings following magic rituals or meetings with the so-called saints of different religions (Hindu sadhus, Sufi dervishes),with incomprehensible and frightening natural phenomena. According to the author’s observations ideas of the unknown in Avadh are codified and structured in Avadh better than in other parts of India. Local people can clearly define if they witness a bhut or a jinn and whether the disease is caused by some witchcraft or other reasons. Perhaps that is due to the presence in the holy town of a persistent tradition of katha, the public presentation of plots from the Ramayana epic in both the narrative and poetic as well as performative forms. But are the events and phenomena in question a miracle for the Avadhvasis, residents of Ayodhya and its environs, or are they so commonplace that they do not surprise or fascinate? That exactly is the subject of the essay, written on the basis of materials collected by the author in Ayodhya during the period of 2010 – 2019. The author would like to express his appreciation to Mr. Alok Sharma (Faizabad) for his advice and cooperation.

HISTOPATHOLOGY OF THE PIGMENTED TUMORAL-LIKE LESIONS OF THE SKIN AT HUE HOSPITAL OF MEDICINE AND PHARMACY UNIVERSITY

2018 ◽  
Vol 8 (4) ◽  
pp. 65-69
Author(s):  
Mao Nguyen Van ◽  
Dong Tran Nam
Keyword(s):  
Key Words ◽  
Definitive Diagnosis ◽  
The Other ◽  
Cell Tumour ◽  
Cross Sectional ◽  
Skin Tumour ◽  
Clinical Appearance ◽  
The Face ◽  
The Common ◽  
Difficult Cases

Background: Pigmented tumour of the skin is one of the common tumour in human including the benign pigmented tumours (more common) called Nevi tumours and the malignant one called melanoma which was less frequent but the most poor in prognosis. In addition, the others not belonging to these group had the same clinical appearance, so the application of histopathology and immunohistochemistry for the definitive diagnosis was indespensible. Objectives: 1. To describe the macroscopic features of the pigmented tumoral-like lesions; 2. To classify the histopathologic types of the pigmented cell tumours and the other pigmented tumours of the skin. Materials and Method: Cross-sectional research on 55 patients diagnosed as pigmented tumoral lesions by clinician, then all definitively diagnosed by histopathology combining the immunohistochemistry in difficult cases. Results: There was no difference in gender, the disease was discovered most common in adult, especially with the age over 51 years old (58.1%). the most region located was in the face accounting for 60%, following the trunk and limbs (14.6%, 12.8% respectively). All 3 malignant melanomas happened in foot. The most common color of the lesions was black (65.4%), the other ones were rose, grey and blue. Histopathology and immunohisthochemistry showed that the true pigmented cell tumours were 52.6% encompassing benign ones (Nevi tumour) (41.8%), melanoma (5.4%) and lentigo (5.4%). 47.4% was not the true pigmented cell tumour including pigmented basocellular carcinoma (36.4%) and the others less common as histiofibromas, acanthoma and papilloma. Conclusion: the pigmented tumoral-like lesions of the skin could be the true pigmented cell tumours and the others, so the application of the histopathology and the immunohistochemistry after the clinical discovery helps to determine and classify the disease definitely and for the best orientation of treatment as well. Key words: skin tumour, benign pigmented tumour (Nevi), malignant pigmented tumour (melanoma), pigmented basocellular carcinoma

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