Uses the Disinfectants against fungi isolated from the homes

2011 ◽  
Vol 35 (1) ◽  
pp. 167-173
Author(s):  
Rusol Muhammedi Al Bahran
Keyword(s):  
Candida Albicans ◽  
Statistical Analysis ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Sodium Hypochlorite ◽  
Frequency Ratio ◽  
Sensitivity Test ◽  
Common Genus

The study included 100 samples collected from different locations of the homes were located in the area of Ali Saleh in Baghdad 6 species were isolated from fungi and the most common genus or species of fungi isolated were Aspergillus fumigatus by frequency ratio of 25.84%, and occurrence ratio of 23%, Penicilium by frequency ratio of 21.34%, and occurrence ratio of 19%, Mucor by frequency ratio 20.22%, and the occurrence ratio of 18%, Candida albicans by frequency ratio of 15.73%, the occurrence ratio of 14%, Rhizopus frequency ratio by 13.48%, the occurrence ratio of 12% and Aspergillus niger frequency ratio by 3.37% and the occurrence ratio of 3%. Then the sensitivity test of disinfectants were studied against fungi isolated by using three disinfectants Chloroxylenol known commercially by (Dettol), Chlorhexidine commercially known by (Hibitane) and Sodium hypochlorite commercially known by (Bleach), and a study for the effected of three concentrations of each disinfectant (5, 2.5, 1.25)%, and the use of statistical analysis (ANOVA) to contrast the differences and Dnken test to the variation in any disinfectant or the most efficient concentrations of other disinfectants were observed that concentrations of 5% was the most efficient of concentrations than (2.5%) and (1.25%). As the disinfectant Dettol was significantly the most efficient from Bleach and Hibitane.

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

scholarly journals Study of fungical conjunctival microbiota of students from the Health Sciences area of the Centro Universitário de Volta Redonda - UniFOA.

2017 ◽  
Vol 2 (3) ◽  
pp. 129
Author(s):  
Aurilio Garcia ◽  
Jobson Bortot ◽  
Marco Antônio Ferraz ◽  
Lívia Mota ◽  
Tassius Borsatto ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Health Sciences ◽  
Fusarium Spp ◽  
Sabouraud Dextrose Agar ◽  
Penicillium Spp

Os autores avaliaram o perfil da microbiota conjuntival fúngica de estudantes da área das Ciências da Saúde do Centro Universitário de Volta Redonda – UniFOA. Foram estudados 200 acadêmicos, sendo 100 pertencentes ao ciclo básico, com restrição ao ambiente hospitalar, e 100 do ciclo clínico, com efetivo contato. O material foi coletado do saco conjuntival e semeado em meio de cultura Sabouraud-dextrose-agar, e mantido em temperatura ambiente.As culturas foram positivas em 26 alunos, correspondendo a 13% do total examinado. Foram isolados os seguintes fungos: 7 (26,92%) Candida albicans, 5 (19,23%) Rhodotorula spp., 6 (23,07%) Penicillium spp., 3 (11,53%) Aspergillus niger, 3 (11,53%) Fusarium spp., e 2 (7,69%) Aspergillus fumigatus. Este resultado é compatível com a maioria dos estudos anteriores realizados por outros autores.

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

scholarly journals Summary Isolation and diagnosis of some fungal types from external ear canal infection cases with sensitivity test to some antifungal agents

2009 ◽  
Vol 33 (1) ◽  
pp. 82-88
Author(s):  
Dalya Abulkareem Abdulshaheed
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Candida Tropicalis ◽  
Antifungal Agents ◽  
Summer Season ◽  
Sensitivity Test ◽  
External Ear ◽  
Ear Canal ◽  
Sensitivity Tests

This research included isolation of some types of fungi of molds and yeasts from cases of inflammation ofexternal ear canal in human from AL- yarmouk Teaching Hospital, by collection of 50 samples from males andfemales and from different ages during summer season , also 10 samples from non infected cases were taken ascontrol. Also sensitivity test of some isolates against some important antifungal agents which commonly usedin the treatments of infection of external ear canale cases.After cultivation of isolates on saboroud dextrose agar, diagnosed as follows :9 isolates of Aspergillus niger, 6 isolates of Aspergillus fumigatus, 3 isolates of Aspergillus flavus, one isolate ofAspergillus terrus, 10 isolates of Candida albicans, 5 isolates of Candida tropicalis, 2 isolates of Candidaparapsilosis.The results of the sensitivity tests of some isolates against the antifungal agents appeared as follows: the isolatesof Aspergillus spp. were very sensitive to Clotrimazole and Econazole, while were less sensitive againstNystatine.Whereas the species of Candida were very sensitive to Nystatine and Econazole, but less sensitive againstClotrimazole.

scholarly journals Study of fungical conjunctival microbiota of students from the Health Sciences area of the Centro Universitário de Volta Redonda - UniFOA.

2017 ◽  
Vol 2 (3) ◽  
pp. 129-132
Author(s):  
Aurilio Garcia ◽  
Jobson Bortot ◽  
Marco Antônio Ferraz ◽  
Lívia Mota ◽  
Tassius Borsatto ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Health Sciences ◽  
Fusarium Spp ◽  
Sabouraud Dextrose Agar ◽  
Penicillium Spp

Os autores avaliaram o perfil da microbiota conjuntival fúngica de estudantes da área das Ciências da Saúde do Centro Universitário de Volta Redonda – UniFOA. Foram estudados 200 acadêmicos, sendo 100 pertencentes ao ciclo básico, com restrição ao ambiente hospitalar, e 100 do ciclo clínico, com efetivo contato. O material foi coletado do saco conjuntival e semeado em meio de cultura Sabouraud-dextrose-agar, e mantido em temperatura ambiente.As culturas foram positivas em 26 alunos, correspondendo a 13% do total examinado. Foram isolados os seguintes fungos: 7 (26,92%) Candida albicans, 5 (19,23%) Rhodotorula spp., 6 (23,07%) Penicillium spp., 3 (11,53%) Aspergillus niger, 3 (11,53%) Fusarium spp., e 2 (7,69%) Aspergillus fumigatus. Este resultado é compatível com a maioria dos estudos anteriores realizados por outros autores.

scholarly journals Antimicrobial activity of Plectranthus amboinicus solvent extracts against Human Pathogenic Bacteria and Fungi

2019 ◽  
Vol 9 (3) ◽  
pp. 36-39
Author(s):  
D. Sivaranjani ◽  
P. Saranraj ◽  
M. Manigandan ◽  
K. Amala
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Essential Oil ◽  
Klebsiella Pneumoniae ◽  
Aspergillus Fumigatus ◽  
Aspergillus Flavus ◽  
Methanol Extract ◽  
Chloroform Extract ◽  
Plectranthus Amboinicus

Plectranthus amboinicus or locally known as bangun-bangun, is an indigenous vegetable which can be freshly eaten. However, the plant is unpopular among local people and being neglected. It has been reported to be traditionally used for medicine to cure common illnesses such as cough, stomachache, headache and skin infection. Based on the potential, a study was conducted to bioprospect the antimicrobial activity of the essential oil. Plectranthus amboinicus essential oil of methanol and chloroform extract was tested against nine bacteria and four fungi i.e., Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Shigella flexneri, Klebsiella pneumoniae, Enterococcus faecal, Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Candida albicans, Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus. The methanol extract of Plectranthus amboinicus showed the maximum antibacterial activity against the bacteria Klebsiella pneumoniae and fungi Candida albicans. The antimicrobial activity of Plectranthus amboinicus was more at 100 mg/ml concentration when compared to 50 mg/ml concentration. Comparatively, the Methanol extract of Plectranthus amboinicus exhibited maximum antimicrobial activity when compared to Chloroform extract. The Plectranthus amboinicus has showed good antimicrobial activity against most of the bacteria and only one fungal yeast Candida albicans. No zone of inhibition was recorded against other fungal isolates like Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Negative DMSO control. Keywords: Plectranthus amboinicus, Bacteria, Fungi, Antimicrobial activity and Well diffusion assay.

A Simple PCR/RFLP Analysis Can Differentiate Between Candida albicans, Aspergillus niger, and Aspergillus fumigatus

2003 ◽  
Vol 24 (3) ◽  
pp. 229-232 ◽  
Author(s):  
Nilgun Isik ◽  
Lewis White ◽  
Rosemary Barnes ◽  
Christopher J. Poynton ◽  
Ken I. Mills
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Rflp Analysis ◽  
Pcr Rflp

Potent Chitin Synthase Inhibitors from Plants

2020 ◽  
Vol 16 (1) ◽  
pp. 58-63
Author(s):  
Amrutha Vijayakumar ◽  
Ajith Madhavan ◽  
Chinchu Bose ◽  
Pandurangan Nanjan ◽  
Sindhu S. Kokkal ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Caenorhabditis Elegans ◽  
Aspergillus Niger ◽  
Small Molecules ◽  
Tip Growth ◽  
Chitin Synthase ◽  
Chitin Synthesis ◽  
Hyphal Tip Growth ◽  
Hyphal Tip

Background: Chitin is the main component of fungal, protozoan and helminth cell wall. They help to maintain the structural and functional characteristics of these organisms. The chitin wall is dynamic and is repaired, rearranged and synthesized as the cells develop. Active synthesis can be noticed during cytokinesis, laying of primary septum, maintenance of lateral cell wall integrity and hyphal tip growth. Chitin synthesis involves coordinated action of two enzymes namely, chitin synthase (that lays new cell wall) and chitinase (that removes the older ones). Since chitin synthase is conserved in different eukaryotic microorganisms that can be a ‘soft target’ for inhibition with small molecules. When chitin synthase is inhibited, it leads to the loss of viability of cells owing to the self- disruption of the cell wall by existing chitinase. Methods: In the described study, small molecules from plant sources were screened for their ability to interfere with hyphal tip growth, by employing Hyphal Tip Burst assay (HTB). Aspergillus niger was used as the model organism. The specific role of these small molecules in interfering with chitin synthesis was established with an in-vitro method. The enzyme required was isolated from Aspergillus niger and its activity was deduced through a novel method involving non-radioactively labelled substrate. The activity of the potential lead molecules were also checked against Candida albicans and Caenorhabditis elegans. The latter was adopted as a surrogate for the pathogenic helminths as it shares similarity with regard to cell wall structure and biochemistry. Moreover, it is widely studied and the methodologies are well established. Results: Out of the 11 compounds and extracts screened, 8 were found to be prospective. They were also found to be effective against Candida albicans and Caenorhabditis elegans. Conclusion: Purified Methyl Ethyl Ketone (MEK) Fraction1 (F1) of Coconut (Cocos nucifera) Shell Extract (COSE) was found to be more effective against Candida albicans with an IC50 value of 3.04 μg/mL and on L4 stage of Caenorhabditis elegans with an IC50 of 77.8 μg/mL.

scholarly journals A Novel Antimicrobial Phenanthrene Alkaloid from Bryopyllum pinnatum

2011 ◽  
Vol 8 (3) ◽  
pp. 1456-1461 ◽  
Author(s):  
Donatus Ebere Okwu ◽  
Fred Uchenna Nnamdi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Klebsiella Pneumonia ◽  
Antimicrobial Studies ◽  
Prevention And Treatment ◽  
Kalanchoe Pinnata ◽  
Isolated Compound

Phenenthrene alkaloid identified as 1-ethanamino 7 hex-1-yne-5I-one phenanthrene was isolated from the ethanolic extract of the leaves ofBryophyllum pinnatum(syn. B. calcinum kalanchoe pinnata) a versatile Nigeria medicinal plant. The structure was elucidated using NMR, IR, UV and MS spectral data. Antimicrobial studies showed that the isolated compound successfully inhibitedPsuedomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Escherichia coli, Candida albicansandAspergillus niger. This result authenticates the use ofbryophyllum pinantumin phytomedicine for disease prevention and treatment of infections.

scholarly journals Dectin-2-Targeted Antifungal Liposomes Exhibit Enhanced Efficacy

mSphere ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Suresh Ambati ◽  
Emma C. Ellis ◽  
Jianfeng Lin ◽  
Xiaorong Lin ◽  
Zachary A. Lewis ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Aspergillus Fumigatus ◽  
Effective Dose ◽  
Cryptococcus Neoformans ◽  
Amphotericin B ◽  
Antifungal Drugs ◽  
Extracellular Matrices ◽  
Content Type ◽  
Order Of Magnitude ◽  
Life Threatening

ABSTRACT Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus cause life-threatening candidiasis, cryptococcosis, and aspergillosis, resulting in several hundred thousand deaths annually. The patients at the greatest risk of developing these life-threatening invasive fungal infections have weakened immune systems. The vulnerable population is increasing due to rising numbers of immunocompromised individuals as a result of HIV infection or immunosuppressed individuals receiving anticancer therapies and/or stem cell or organ transplants. While patients are treated with antifungals such as amphotericin B, all antifungals have serious limitations due to lack of sufficient fungicidal effect and/or host toxicity. Even with treatment, 1-year survival rates are low. We explored methods of increasing drug effectiveness by designing fungicide-loaded liposomes specifically targeted to fungal cells. Most pathogenic fungi are encased in cell walls and exopolysaccharide matrices rich in mannans. Dectin-2 is a mammalian innate immune membrane receptor that binds as a dimer to mannans and signals fungal infection. We coated amphotericin-loaded liposomes with monomers of Dectin-2’s mannan-binding domain, sDectin-2. sDectin monomers were free to float in the lipid membrane and form dimers that bind mannan substrates. sDectin-2-coated liposomes bound orders of magnitude more efficiently to the extracellular matrices of several developmental stages of C. albicans, C. neoformans, and A. fumigatus than untargeted control liposomes. Dectin-2-coated amphotericin B-loaded liposomes reduced the growth and viability of all three species more than an order of magnitude more efficiently than untargeted control liposomes and dramatically decreased the effective dose. Future efforts focus on examining pan-antifungal targeted liposomal drugs in animal models of fungal diseases. IMPORTANCE Invasive fungal diseases caused by Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus have mortality rates ranging from 10 to 95%. Individual patient costs may exceed $100,000 in the United States. All antifungals in current use have serious limitations due to host toxicity and/or insufficient fungal cell killing that results in recurrent infections. Few new antifungal drugs have been introduced in the last 2 decades. Hence, there is a critical need for improved antifungal therapeutics. By targeting antifungal-loaded liposomes to α-mannans in the extracellular matrices secreted by these fungi, we dramatically reduced the effective dose of drug. Dectin-2-coated liposomes loaded with amphotericin B bound 50- to 150-fold more strongly to C. albicans, C. neoformans, and A. fumigatus than untargeted liposomes and killed these fungi more than an order of magnitude more efficiently. Targeting drug-loaded liposomes specifically to fungal cells has the potential to greatly enhance the efficacy of most antifungal drugs.

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