Bromocresol Purple TS

2021 ◽  
Keyword(s):  
Bromocresol Purple

Correction: Evaluation of Root pH Change Through Gel Containing pH-sensitive Indicator Bromocresol Purple

BIO-PROTOCOL ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
Aparecida Silva ◽  
Keini Dressano ◽  
Paulo Ceciliato ◽  
Juan Carlos Guerrero-Abad ◽  
Daniel Moura
Keyword(s):  
Ph Sensitive ◽  
Sensitive Indicator ◽  
Bromocresol Purple ◽  
Ph Change

scholarly journals Molecularly imprinted electrospun fiber membrane for colorimetric detection of hexanoic acid

e-Polymers ◽  
2021 ◽  
Vol 21 (1) ◽  
pp. 500-510
Author(s):  
Xiaoguang Ying ◽  
Jieyuan He ◽  
Xiao Li
Keyword(s):  
Low Cost ◽  
Electrospun Fiber ◽  
Colorimetric Detection ◽  
Hexanoic Acid ◽  
Bromocresol Purple ◽  
Fiber Membrane ◽  
Color Changes ◽  
Before And After ◽  
The Difference ◽  
Imagej Software

Abstract An imprinted electrospun fiber membrane was developed for the detection of volatile organic acids, which are key components of human body odor. In this study, hexanoic acid (HA) was selected as the target, polymethyl methacrylate (PMMA) was used as the substrate, and colorimetric detection of HA was achieved by a bromocresol purple (BCP) chromogenic agent. The results showed that the morphology of the fiber membrane was uniform and continuous, and it showed excellent selectivity and specificity to HA. Photographs of the color changes before and after fiber membrane adsorption were recorded by a camera and quantified by ImageJ software by the difference in gray value (ΔGray). This method is simple, intuitive, and low cost and has great potential for application in human odor analysis.

scholarly journals Partial purification and biochemical characterization of a new highly acidic NYSO laccase from Alcaligenes faecalis

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Soad A. Abdelgalil ◽  
Ahmad R. Attia ◽  
Reyed M. Reyed ◽  
Nadia A. Soliman
Keyword(s):  
Enzyme Activity ◽  
Sodium Dodecyl ◽  
Alcaligenes Faecalis ◽  
Maximum Activity ◽  
Sodium Oxalate ◽  
Partial Purification ◽  
Bromophenol Blue ◽  
Bromocresol Purple ◽  
Laccase Enzyme

Abstract Background Due to the multitude industrial applications of ligninolytic enzymes, their demands are increasing. Partial purification and intensive characterization of contemporary highly acidic laccase enzyme produced by an Egyptian local isolate designated Alcaligenes faecalis NYSO were studied in the present investigation. Results Alcaligenes faecalis NYSO laccase has been partially purified and intensively biochemically characterized. It was noticed that 40–60% ammonium sulfate saturation showed maximum activity. A protein band with an apparent molecular mass of ~ 50 kDa related to NYSO laccase was identified through SDS-PAGE and zymography. The partially purified enzyme exhibited maximum activity at 55 °C and pH suboptimal (2.5–5.0). Remarkable activation for enzyme activity was recognized after 10-min exposure to temperatures (T) 50, 60, and 70 °C; time elongation caused inactivation, where ~ 50% of activity was lost after a 7-h exposure to 60 °C. Some metal ions Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Cd2+, Cr2+, and Mg2+ caused strong stimulation for enzyme activity, but Fe2+ and Hg2+ reduced the activity. One millimolar of chelating agents [ethylenediamine tetraacetic acid (EDTA), sodium citrate, and sodium oxalate] caused strong activation for enzyme activity. Sodium dodecyl sulfate (SDS), cysteine-HCl, dithiothreitol (DTT), β-mercaptoethanol, thioglycolic acid, and sodium azide caused strong inhibition for NYSO laccase activity even at low concentration. One millimolar of urea, imidazole, kojic acid, phenylmethylsulfonyl fluoride (PMSF), H2O2, and Triton X-100 caused activation. The partially purified NYSO laccase had decolorization activity towards different dyes such as congo red, crystal violet, methylene blue, fast green, basic fuchsin, bromophenol blue, malachite green, bromocresol purple eriochrome black T, and Coomassie Brilliant Blue R-250 with various degree of degradation. Also, it had a vast range of substrate specificity including lignin, but with high affinity towards p-anisidine. Conclusion The promising properties of the newly studied laccase enzyme from Alcaligenes faecalis NYSO strain would support several industries such as textile, food, and paper and open the possibility for commercial use in water treatment. It will also open the door to new applications due to its ligninolytic properties in the near future.

scholarly journals Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- Prospect of a Novel Enzyme Tag for Immunoassay Systems

2017 ◽  
Vol 5 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Premalatha Shetty ◽  
Avila D’Souza ◽  
Geethu CP
Keyword(s):  
Brassica Oleracea ◽  
Temperature Stability ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Bromocresol Purple ◽  
Significant Extent ◽  
Periodate Treatment ◽  
Ammonium Sulfate Saturation ◽  
Tissue Antigens

Peroxidase tagged proteins are being used successfully as immune-histological probes for the demonstration of tissue antigens, and in enzyme amplified immunoassay systems for the quantitative determination of soluble and insoluble antigens. The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. Peroxidase extracted from the bulbs of Brassica oleracea gongylodes was salted out at 40-80% ammonium sulfate saturation and activated by treatment with 1-Fluoro-2,4-dinitro benzene (FDNB) and periodate. Treatment with 0.08% FDNB and 12.5mM periodate was optimized for activation of the enzyme. The treated enzyme was found to conjugate successfully to immunoglobulin fractions harvested from egg yolk (IgY), human plasma and goat serum. Enzyme conjugated to IgY fraction showed improvement in its pH stability and temperature stability. The affinity of the enzyme for its substrate phenol did not alter to a significant extent upon activation and conjugation. The conjugates exhibited high affinity towards phenol, bromocresol purple and bromothymol blue in comparison to HRP conjugates prepared using the same protocol. Int. J. Appl. Sci. Biotechnol. Vol 5(1): 59-65

A New Photometric Assay with Bromocresol Purple for Testing in vitro Antitrichomonal Activity in Aerobic Environment

2011 ◽  
Vol 49 (01) ◽  
pp. 51-55 ◽  
Author(s):  
Philippe Loiseau ◽  
Christian Bories ◽  
Albertine Sanon
Keyword(s):  
Bromocresol Purple ◽  
Photometric Assay

scholarly journals Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5007
Author(s):  
Małgorzata Szultka-Młyńska ◽  
Daria Janiszewska ◽  
Paweł Pomastowski ◽  
Michał Złoch ◽  
Wojciech Kupczyk ◽  
...  
Keyword(s):  
Brain Heart Infusion ◽  
Culture Conditions ◽  
Clinical Samples ◽  
Bromocresol Purple ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Enterococcus Spp ◽  
Identification Of Bacteria ◽  
Staphylococcus Spp

The bacterial infection of post-operative wounds is a common health problem. Therefore, it is important to investigate fast and accurate methods of identifying bacteria in clinical samples. The aim of the study was to analyse the use of the MALDI-TOF MS technique to identify microorganism wounds that are difficult to heal. The most common bacteria are Escherichia coli, Staphylococcus spp., and Enterococcus spp. We also demonstrate the effect of culture conditions, such as the used growth medium (solid: Brain Heart Infusion Agar, Mueller Hilton Agar, Glucose Bromocresol Purple Agar, and Vancomycin Resistance Enterococci Agar Base and liquid: Tryptic Soy Broth and BACTEC Lytic/10 Anaerobic/F), the incubation time (4, 6, and 24h), and the method of the preparation of bacterial protein extracts (the standard method based on the Bruker guideline, the Sepsityper method) to identify factors and the quality of the obtained mass spectra. By comparing the protein profiles of bacteria from patients not treated with antibiotics to those treated with antibiotics based on the presence/absence of specific signals and using the UniProt platform, it was possible to predict the probable mechanism of the action of the antibiotic used and the mechanism of drug resistance.

scholarly journals Evaluation of sodium lauryl sulfate for the development of cow-side mastitis screening test

2021 ◽  
pp. 2290-2295
Author(s):  
Nobonita Sarker Tanni ◽  
Md. Shafiul Islam ◽  
Mojahidul Kabir ◽  
Mst. Sonia Parvin ◽  
Md. Amimul Ehsan ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Sodium Lauryl Sulfate ◽  
Early Stage ◽  
High Sensitivity ◽  
Subclinical Mastitis ◽  
Screening Tests ◽  
Bromocresol Purple ◽  
Lauryl Sulfate ◽  
Operating Characteristics ◽  
Milk Samples

Background and Aim: Subclinical mastitis (SCM) is an economically important disease for dairy cattle worldwide; therefore, regular screening is imperative to detect SCM at an early stage so as to control it. The study was conducted to compare the test characteristics of sodium lauryl sulfate (SLS) as a test reagent to detect SCM in dairy cows. Materials and Methods: First, 106 milk samples of dairy cows were subjected to available indirect screening tests (white side test [WST], surf field mastitis test, Leucocytest, and Immucell) considering somatic cell count (SCC) as gold standard test. Then 273 milk samples were allowed to react with different concentrations of SLS with or without sodium hydroxide (NaOH) and indicators (bromothymol blue [BTB] and bromocresol purple [BCP]). Results: SLS with or without NaOH yielded best reaction with the milk samples similar to Leucocytest. It was observed that the reaction of milk samples with SLS added with indicators (BTB and BCP) was easier to visualize than without indicators. SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP had high sensitivity, specificity, and substantial agreement with SCC. The area under the receiver operating characteristics curve of SLS 2% with BCP and SLS 3%+NaOH 1.5% with BTB was 0.917 and 0.875, respectively. Conclusion: It may be concluded that SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP may be the potential reagents for the development of an effective cow-side test to detect SCM, as the main ingredient SLS is considerably cheap and readily available in developing countries.

Sign in / Sign up

Export Citation Format

Share Document