scholarly journals Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum)

2017 ◽  
Vol 9 (2) ◽  
pp. 1085-1089
Author(s):  
Sonia Sood ◽  
Sanjay Jambhulkar ◽  
Yudhvir Singh ◽  
Nivedita Gupta ◽  
Saloni Sharma

An investigation entitled “Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum)” was conducted during kharif (summer-rainy season) 2012 and 2013 at Experimental farm of the Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, H.P., India. Healthy seeds of California Wonder of bell pepper were exposed to physical mutagen Gamma rays using 60CO as a source of radiation at Mutation Breeding Centre, Department of Biotechnology, BARC Trombay, Mumbai and chemical mutagen EMS (Ethyl Methane Sulphonate) to obtain the spectrum and frequency of chlorophyll mutations in M2 generation. The M1 generation was produced from these mutagen treated seeds. Several unique and interesting chlorophyll and viable mutants were obtained in M2 generation. In M2 generation, gamma rays induced higher proportion of chlorophyll mutants then EMS. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses/concentrations. Four different types of chlorophyll mutants namely xantha, yellow xantha, chlorina and viridis were induced. Out of these mutants, chlorina and viridis were most frequent and were produced even in lower doses/concentrations while yellow xantha was least frequent and produced only in higher doses. The highest frequency of chlorophyll mutations (18.8 %) was reported in the 22 kR of gamma dose, while the lowest (0.80 %) frequency of chlorophyll mutations was found in the treatment of 1.0 % EMS. There was a dose dependent increase in the spectrum and frequency of chlorophyll mutations. These chlorophyll mutants induced by gamma radiation and EMS could be used in mutation breeding programme for inducing viable mutations for improvement of bell pepper varieties.

Author(s):  
K.D. Savant

Background: Mutation breeding is one of the cheapest and surest method of plant breeding. In mutation breeding program effectiveness and efficiency of mutagens could be helpful to realize the spectrum of desirable mutations in the treated populations. The effectiveness and efficiency of two mutagens EMS and gamma rays were studied in two varieties of cowpea [Vigna unguiculata (L.)Walp.]. Methods: In the present investigation, the seeds of two varieties of cowpea, phulepandhari and VCM-8 were treated with gamma rays and EMS to obtain the spectrum and frequency of chlorophyll mutations in M2 generation. Mutagenic effectiveness and efficiency were calculated using frequency of chlorophyll mutations.Result: The individual treatment of EMS was found to be more efficient than gamma rays to induce chlorophyll mutants. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses. Four different types of chlorophyll mutants, such as, chlorina albina, xantha and viridis were induced with effect of mutagens. The highest frequency of chlorophyll mutations was reported in the gamma rays. There was a dose dependent increase in the spectrum and frequency of chlorophyll mutations. The lower dosages of gamma rays and EMS were proved to be most effective and efficient. Among two mutagens used EMS was much more effective than gamma rays in both the varieties viz. variety phulepandhari and VCM-8. 


2016 ◽  
Vol 1 (1) ◽  
pp. 49 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan

A relative study of frequency and spectrum of chlorophyll mutations induced by mutagens in M2 generation was made with chickpea (Cicer arietinum (L). Variety ‘CO-4’. The treatments include different doses/concentrations of Gamma rays (20, 30, 40, 50 and 60kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM). From the study, the overall frequencies and spectrum of five types of induced chlorophyll mutants Viridis (0.55), Xantha (0.46), Chlorina (0.45), Albina (0.43) and Tigrina (0.35) were observed. The frequency of chlorophyll mutation was increased with increasing concentrations up to a level, beyond it declined in both the mutagens. And the chlorophyll frequency was found in the order of viridis > xantha >chlorina> Albina >tigrina. The chemical mutagen, EMS was found to be more effective in inducing chlorophyll mutations than gamma rays in Chick pea.


2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Kalyani R Gunjal ◽  
A.D. More

The present investigation was undertaken to study the frequency of chlorophyll deficient sectors and chlorophyll mutants induced by Gamma rays at the dosage of 240Gy,300Gy,360Gy,420Gy, Ethyl Methene Sulphate (EMS) at the concentrations of 0.25%,0.50%,0.75%,1% and the Combination of both (Gamma rays and EMS) like 240Gy+1%, 300Gy+0.75%, 360Gy+0.50%, 420Gy+0.25% in the variety ‘King of Garden’ Phaseolus lunatus (lima bean).The chlorophyll mutants were observed in all the three mutagenic treatments in the lima bean. These mutants were White (Albina), Yellow (Xantha), Yellow green (Chlorina) and Light green (Viridis). They were screened after 10-15 days of old seedling and were found at the margin of the leaflet or the entire leaflet.Induced mutation in the sectors of the leaves resulted into chlorophyll chimeric plants. The scoring of chlorophyll mutation frequency in M2 generation was one of the most reliable measures for evaluating the mutagenic induced genetic altercation of the mutagen treatments used in the ideotype. The frequency of chlorophyll mutants increased with the increase in the dose/concentration of the mutants. They can be screened and enhanced through mutation breeding for enormous yield.


Irriga ◽  
2003 ◽  
Vol 8 (2) ◽  
pp. 120-131 ◽  
Author(s):  
Francisco Fernando Noronha Marcussi ◽  
Roberto Lyra Villas Bôas

TEORES DE MICRONUTRIENTES NO DESENVOLVIMENTO DA PLANTA DE PIMENTÃO SOB FERTIRRIGAÇÃO   Francisco Fernando Noronha MarcussiDepartamento de Engenharia Rural, Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: [email protected] Lyra Villas BôasDepartament de Recursos Faculdade de Ciências Agronômicas,Universidade Estadual Paulista, Botucatu, SP. CP 237, CEP 18603-970. E-mail: [email protected]  1 RESUMO  Neste estudo, objetivou-se determinar os teores de micronutrientes (Fe, Zn, Mn, B e Cu) nos diferentes órgãos do híbrido de pimentão Elisa, sob fertirrigação em ambiente protegido, em oito épocas de coleta de planta. O experimento foi desenvolvido nas dependências do Departamento de Recursos Naturais - Ciência do Solo, FCA, Botucatu/SP. Constou o experimento de 8 tratamentos (épocas de coleta de planta – 0, 20, 40, 60, 80, 100, 120, e 140 dias após o transplante das mudas) e 4 repetições, sendo cada repetição formada por 4 plantas. Em cada etapa obteve-se os teores dos micronutrientes no fruto, folha, caule e raiz e na planta como um todo. A fertilização no ciclo estudado, 140 dias, foi feita via fertirrigação localizada. Os resultados mostram que os teores de micronutrientes encontrados na planta de pimentão seguem a seqüência: Fe>Zn>Mn>B>Cu. Nos resultados são apresentadas as tabelas com os teores de Fe, Zn, Mn, B e Cu (Tukey a 5%) em cada órgão da planta e da planta como um todo, nas diferentes épocas de avaliação e, os gráficos mostrando o comportamento dos teores de Zn, Mn, B e Cu.  UNITERMOS: Fertirrigação, concentrações de micronutrientes, Pimentão, Capsicum annuum L. e nutrição mineral.   MACUSSI, F.F.N.; VILLAS BÔAS, R.L MICRONUTRIENT CONCENTRATION IN THE BELL PEPPER PLANT DEVELOPMENT UNDER FERTIRRIGATION   2 ABSTRACT  This study aimed to determine the micronutrient contents (Fe, Zn, Mn, B and Cu) in different organs of Elisa bell pepper hybrid under fertirrigation in protected environment in eight harvesting seasons. The experiment was carried out in the Natural Resource Department – Soil Science, FCA, Botucatu. SP, and  consisted of 8 treatments (harvesting season – 0, 20, 40, 60, 80, 100 and 140 days after seedling transplant), and 4 replications, each one composed of 4 plants. In each growth period the micronutrient content in the fruit, leaf, stem, root and  plant as a whole was determined. The fertilization in the study period i.e., 140 days, was performed by local fertirrigation. The results showed that the micronutrient levels found in the bell pepper plant followed the decreasing order: Fe>Zn>Mn> B>Cu. The tables present the Fe, Zn, Mn, B and Cu (Tukey Test at 5%) contents in each plant organ and in the entire plant at different evaluation periods. The charts show the Zn, Mn, B and Cu content variation.  KEYWORDS: fertirrigation, micronutrient concentration, bell pepper, Capsicum annuum L. and mineral nutrition. 


1969 ◽  
Vol 71 (3) ◽  
pp. 337-340
Author(s):  
Jaime Escudero ◽  
Guillermo Fornaris-Rullán ◽  
Elvin Caraballo

YIELD AND TOLERANCE OF BELL PEPPER (CAPSICUM ANNUUM L.) CULTIVARS TO POTATO VIRUS Y ISOLATE FROM PUERTO RICO


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