scholarly journals Biodegradation of Crude Oil Through Biosurfactant Producing Bacterial Strains Isolated from Oily Sludge

2020 ◽  
Vol 3 (3) ◽  
pp. 01-06
Author(s):  
Veeranna Channashettar ◽  
Shaili Srivastava ◽  
Banwari Lal
Keyword(s):  
Crude Oil ◽  
Oily Sludge ◽  
Bacterial Strains

scholarly journals Energy and Resource Utilization of Refining Industry Oil Sludge by Microwave Treatment

2020 ◽  
Vol 12 (17) ◽  
pp. 6862
Author(s):  
Chien Li Lee ◽  
Cheng-Hsien Tsai ◽  
Chih-Ju G. Jou
Keyword(s):  
Crude Oil ◽  
Microwave Power ◽  
Petroleum Refining ◽  
Microwave Treatment ◽  
Field Energy ◽  
Oily Sludge ◽  
Sludge Sample ◽  
Volatile Hydrocarbons ◽  
Benzene Toluene ◽  
Oil Tank

The oily sludge from crude oil contains hazardous BTEX (benzene, toluene, ethylbenzene, xylene) found in the bottom sediment of the crude oil tank in the petroleum refining plant. This study uses microwave treatment of the oily sludge to remove BTEX by utilizing the heat energy generated by the microwave. The results show that when the oily sludge sample was treated for 60 s under microwave power from 200 to 300 W, the electric field energy absorbed by the sample increased from 0.17 to 0.31 V/m and the temperature at the center of the sludge sample increased from 66.5 °C to 96.5 °C. In addition, when the oily sludge was treated for 900 s under microwave power 300 W, the removal rates were 98.5% for benzene, 62.8% for toluene, 51.6% for ethylbenzene, and 29.9% for xylene. Meanwhile, the highest recovery rates of light volatile hydrocarbons in sludge reached 71.9% for C3, 71.3% for C4, 71.0% for C5, and 78.2% for C6.

scholarly journals CRUDE OIL BIOREMEDIATION BY INDIGENOUS BACTERIA ISOLATED FROM OILY SLUDGE

2016 ◽  
Vol 78 (11-2) ◽  
Author(s):  
Nur Hafizah Azizan ◽  
Kasing Ak Apun ◽  
Lesley Maurice Bilung ◽  
Micky Vincent ◽  
Hairul Azman Roslan ◽  
...  
Keyword(s):  
Crude Oil ◽  
Enrichment Culture ◽  
Molecular Techniques ◽  
Oily Sludge ◽  
Minimal Salt Medium ◽  
Alkane Degradation ◽  
Growth Study ◽  
The Third ◽  
Cell Counts ◽  
Degrading Bacteria

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.

Bioremediation potential of native hydrocarbon degrading bacterial strains in crude oil contaminated soil under microcosm study

2014 ◽  
Vol 94 ◽  
pp. 79-89 ◽  
Author(s):  
Abhijit Sarma Roy ◽  
Reshita Baruah ◽  
Maina Borah ◽  
Anil Kumar Singh ◽  
Hari Prasanna Deka Boruah ◽  
...  
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Bacterial Strains ◽  
Microcosm Study

scholarly journals Characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria: potential application in bioremediation

2018 ◽  
Vol 17 (2) ◽  
pp. 103-114 ◽  
Author(s):  
Pranjal Bharali ◽  
Salam Pradeep Singh ◽  
Yasir Bashir ◽  
Nipu Dutta ◽  
Bolin Kumar Konwar ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oil Recovery ◽  
Potential Application ◽  
Elevated Temperatures ◽  
Extreme Temperature ◽  
Chemical Characterization ◽  
Bacterial Strains ◽  
Hydrocarbonoclastic Bacteria ◽  
Rdna Sequencing ◽  
Thermo Stability

Abstract Petroleum and hydrocarbons contamination can be remediated by physical, chemical or biological methods. Among these, in situ bioremediation is considered to be environmentally friendly because it restores the soil structure, requires less energy input and involves the notable removal after degradation of biosurfactant. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques. The results clearly demonstrate the capability of utilizing hydrocarbon and biosurfactant produced by the bacterial strains. 16S rDNA sequencing followed by BLAST analysis revealed their similarity to Pseudomonas aeruginosa. The physico-chemical characterization of the biosurfactants revealed significant surface properties with stability at extreme temperature conditions (up to 121˚C), pH (5 - 8) and salinity (up to 4 %). Further, the mass spectrometry confirmed predominance of di-rhamnolipids in biosurfactant mixtures. The biosurfactants were found to be efficient in the removal of crude oil from the contaminated sand suggesting its applicability in bioremediation technology. Further, improved discharge of crude oil at elevated temperatures also confirms their thermo-stability which, could be exploited in microbial enhanced oil recovery processes. Thus, the applications of biosurfactants produced by the indigenous hydrocarbonoclastic strains appeared to be advantageous for bioremediation of petroleum-contaminated environments.

scholarly journals Biotechnological Potentials of Microbe Assisted Eco-Recovery of Crude Oil Impacted Environment

2021 ◽  
Author(s):  
Chioma Bertha Ehis-Eriakha ◽  
Stephen Eromosele Akemu ◽  
Simon Obgaji Otumala ◽  
Chinyere Augusta Ajuzieogu
Keyword(s):  
Crude Oil ◽  
Environmental Sustainability ◽  
Phthalate Esters ◽  
Genetically Engineered ◽  
Bacterial Strains ◽  
Yield Increase ◽  
Enzymatic Systems ◽  
Polycyclic Aromatic ◽  
Challenging Situation ◽  
Contamination Concentration

Globally, the environment is facing a very challenging situation with constant influx of crude oil and its derivatives due to rapid urbanization and industrialization. The release of this essential energy source has caused tremendous consequences on land, water, groundwater, air and biodiversity. Crude oil is a very complex and variable mixture of thousands of individual compounds that can be degraded with microbes with corresponding enzymatic systems harboring the genes. With advances in biotechnology, bioremediation has become one of the most rapidly developing fields of environmental restoration, utilizing microorganisms to reduce the concentration and toxicity of various chemical pollutants, such as petroleum hydrocarbons, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, phthalate esters, nitroaromatic compounds and industrial solvents. Different remediation methods have been introduced and applied with varied degrees of success in terms of reduction in contamination concentration without considering ecotoxicity and restoration of biodiversity. Researchers have now developed methods that consider ecotoxicology, environmental sustainability and ecorestoration in remediation of crude oil impacted sites and they are categorized as biotechnological tools such as bioremediation. The approach involves a natural process of microorganisms with inherent genetic capabilities completely mineralizing/degrading contaminants into innocuous substances. Progressive advances in bioremediation such as the use of genetically engineered microbes have become an improved system for empowering microbes to degrade very complex recalcitrant substances through the modification of rate-limiting steps in the metabolic pathway of hydrocarbon degrading microbes to yield increase in mineralization rates or the development of completely new metabolic pathways incorporated into the bacterial strains for the degradation of highly persistent compounds. Other areas discussed in this chapter include the biosurfactant-enhanced bioremediation, microbial and plant bioremediation (phytoremediation), their mechanism of action and the environmental factors influencing the processes.

scholarly journals The Efficiency of Acinetobacter Radioresistens Strain KA2 Isolated From Oily Sludge for Degrading of Crude Oil ‎

2019 ◽  
pp. 78-89
Author(s):  
Mohammad Saeed Poorsoleiman ◽  
Seyed Ahmad Hosseini Seyed ◽  
Alireza Etminan ◽  
Hamid Abtahi ◽  
Ali Koolivand
Keyword(s):  
Crude Oil ◽  
Oily Sludge ◽  
Acinetobacter Radioresistens

scholarly journals Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

2020 ◽  
Vol 17 (22) ◽  
pp. 8446
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Galal Ali Esmail ◽  
Mariadhas Valan Arasu
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Crude Oil ◽  
Solid State Fermentation ◽  
Contaminated Soils ◽  
Quadratic Model ◽  
Subtilis Strain ◽  
Natural Environments ◽  
Bacterial Strains ◽  
Enhanced Production

Crude oil and its derivatives are the most important pollutants in natural environments. Bioremediation of crude oil using bacteria has emerged as a green cleanup approach in recent years. In this study, biosurfactant-producing Bacillus subtilis strain Al-Dhabi-130 was isolated from the marine soil sediment. This organism was cultured in solid-state fermentation using agro-residues to produce cost-effective biosurfactants for the bioremediation of crude-oil contaminated environments. Date molasses improved biosurfactant production and were used for further optimization studies. The traditional “one-variable-at-a-time approach”, “two-level full factorial designs”, and a response surface methodology were used to optimize the concentrations of date molasses and nutrient supplements for surfactant production. The optimum bioprocess conditions were 79.3% (v/w) moisture, 34 h incubation period, and 8.3% (v/v) glucose in date molasses. To validate the quadratic model, the production of biosurfactant was performed in triplicate experiments, with yields of 74 mg/g substrate. These findings support the applications of date molasses for the production of biosurfactants by B. subtilis strain Al-Dhabi-130. Analytical experiments revealed that the bacterial strain degraded various aromatic hydrocarbons and n-alkanes within two weeks of culture with 1% crude oil. The crude biosurfactant produced by the B. subtilis strain Al-Dhabi-130 desorbed 89% of applied crude oil from the soil sample. To conclude, biosurfactant-producing bacterial strains can increase emulsification of crude oil and support the degradation of crude oil.

Presence of Hydrocarbon Degrading Bacteria in Contaminated Soil Collected From Various Fuel Station in Bhilai, Chhattisgarh

2021 ◽  
Vol 9 (11) ◽  
pp. 1802-1804
Author(s):  
Lumeshwari Sahu
Keyword(s):  
Environmental Factors ◽  
Crude Oil ◽  
Large Scale ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Degrading Bacteria ◽  
Microbial Remediation ◽  
Petroleum Oil ◽  
Growth Ability

Abstract: In this study, we isolated seven strains (termed BY1–7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Among 45 bacterial colonies one bacterial strain was identified based on the cultural, morphological and biochemical characteristics. The isolated bacterium was then subjected to a preliminary assessment of their crude oil after 48 hours of incubation on nutrient agar plates overlaid with 100 ML of petroleum crude oil, the zone of clearance was observed. The isolated bacteria showed 35% petrol degradation, whereas a relatively high oil degradation rate, almost 40% was observed when the bacterium was acclimatized. The selected bacterial strains crude oil resistance was analysed based on the growth ability on the crude oil containing mediums. This strain was identified as Brevibacterium brevis. After inoculation, growth ability was measured and the highest percentage of petrol degradation occurred at temperature 37 °C with the value 30.8%. Bacteria displaying such capabilities are often exploited for the bio-remediation of petroleum oil contaminated environments. Recently, microbial remediation technology has developed rapidly and achieved major gains. However, this technology is not omnipotent. It is affected by many environmental factors that hinder its practical application, limiting the large-scale application of the technology. Keywords: Petroleum hydrocarbon-degrading Bacteria, Petroleum oil, Bio-remediation, Bacterial consortia, Environmental factors, Enzymes.

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