scholarly journals The Efficiency of Acinetobacter Radioresistens Strain KA2 Isolated From Oily Sludge for Degrading of Crude Oil ‎

2019 ◽  
pp. 78-89
Author(s):  
Mohammad Saeed Poorsoleiman ◽  
Seyed Ahmad Hosseini Seyed ◽  
Alireza Etminan ◽  
Hamid Abtahi ◽  
Ali Koolivand
Keyword(s):  
Crude Oil ◽  
Oily Sludge ◽  
Acinetobacter Radioresistens

scholarly journals Energy and Resource Utilization of Refining Industry Oil Sludge by Microwave Treatment

2020 ◽  
Vol 12 (17) ◽  
pp. 6862
Author(s):  
Chien Li Lee ◽  
Cheng-Hsien Tsai ◽  
Chih-Ju G. Jou
Keyword(s):  
Crude Oil ◽  
Microwave Power ◽  
Petroleum Refining ◽  
Microwave Treatment ◽  
Field Energy ◽  
Oily Sludge ◽  
Sludge Sample ◽  
Volatile Hydrocarbons ◽  
Benzene Toluene ◽  
Oil Tank

The oily sludge from crude oil contains hazardous BTEX (benzene, toluene, ethylbenzene, xylene) found in the bottom sediment of the crude oil tank in the petroleum refining plant. This study uses microwave treatment of the oily sludge to remove BTEX by utilizing the heat energy generated by the microwave. The results show that when the oily sludge sample was treated for 60 s under microwave power from 200 to 300 W, the electric field energy absorbed by the sample increased from 0.17 to 0.31 V/m and the temperature at the center of the sludge sample increased from 66.5 °C to 96.5 °C. In addition, when the oily sludge was treated for 900 s under microwave power 300 W, the removal rates were 98.5% for benzene, 62.8% for toluene, 51.6% for ethylbenzene, and 29.9% for xylene. Meanwhile, the highest recovery rates of light volatile hydrocarbons in sludge reached 71.9% for C3, 71.3% for C4, 71.0% for C5, and 78.2% for C6.

scholarly journals CRUDE OIL BIOREMEDIATION BY INDIGENOUS BACTERIA ISOLATED FROM OILY SLUDGE

2016 ◽  
Vol 78 (11-2) ◽  
Author(s):  
Nur Hafizah Azizan ◽  
Kasing Ak Apun ◽  
Lesley Maurice Bilung ◽  
Micky Vincent ◽  
Hairul Azman Roslan ◽  
...  
Keyword(s):  
Crude Oil ◽  
Enrichment Culture ◽  
Molecular Techniques ◽  
Oily Sludge ◽  
Minimal Salt Medium ◽  
Alkane Degradation ◽  
Growth Study ◽  
The Third ◽  
Cell Counts ◽  
Degrading Bacteria

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.

Research on Treatment Technology as Resource of Polymer-Containing Oily Sludge

2014 ◽  
Vol 955-959 ◽  
pp. 2677-2682 ◽  
Author(s):  
Xian Qing Yin ◽  
Fei Fei Hu ◽  
Bo Jing ◽  
Jian Zhang ◽  
Xi Zhou Shen ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oil Recovery ◽  
Sewage Treatment ◽  
Oil Field ◽  
Oily Sludge ◽  
Processing Plant ◽  
Environment Protection ◽  
Treatment Technology ◽  
Oil Water ◽  
Separating Agent

With the rapid implementation of polymer flooding in Bohai oil field, the produced liquid includes large amount of polymer-containing oily sludge reversed increases year by year. The polymer-containing oily sludge accumulates at the terminal processing plant, which not only obviously degrades the performance of sewage treatment instruments and blocks the oil/water separators, but also has a bad impact on environment. Using thermal chemical treatment technology with dynamical separating agent and optimizing separation conditions, the completed processing technology is obtained as follow: thermal chemical reaction, separation on standing, crude oil recovery and recycling of waste water. The recovery rate of crude oil from the samples treatment is over 94%. The obtained technology plays an important role in recycling of source, environment protection and technical support of increasing produced liquid.

scholarly journals Biodegradation of Crude Oil Through Biosurfactant Producing Bacterial Strains Isolated from Oily Sludge

2020 ◽  
Vol 3 (3) ◽  
pp. 01-06
Author(s):  
Veeranna Channashettar ◽  
Shaili Srivastava ◽  
Banwari Lal
Keyword(s):  
Crude Oil ◽  
Oily Sludge ◽  
Bacterial Strains

APPLICATION OF BIOSURFACTANT IN OIL SPILL MANAGEMENT

1993 ◽  
Vol 1993 (1) ◽  
pp. 503-504
Author(s):  
Asha Juwarkar ◽  
P. Sudhakar Babu ◽  
Kirti Mishra ◽  
Megha Deshpande
Keyword(s):  
Surface Tension ◽  
Interfacial Tension ◽  
Crude Oil ◽  
Oil Spill ◽  
Oily Sludge ◽  
Stable Emulsion ◽  
Oil Emulsion ◽  
Surface Tension Reduction ◽  
Surface Active ◽  
Oil Spill Cleanup

ABSTRACT Surfactants are surface active agents which reduce surface tension and interfacial tension between two immiscible phases and help in emulsification. Toxicity, nonbiodegradability, and limited structural types of chemical surfactants have initiated the need for effective substitutes. Biosurfactants, which are synthesized by specific microbial cultures, have surface active properties comparable to chemical surfactants. They are compounds that can help in oil spill cleanup operations without presenting the problems posed by chemical surfactants. Two bacterial cultures were isolated from oil-contaminated soil and were used for biosurfactant production. The biosurfactants produced by the Bacillus licheniformis, BS1, and Pseudomonas aeruginosa, BS2, in mineral media containing glucose as the carbon source belong to the class of lipoprotein and glycolipid, respectively. They were found to reduce the surface and interfacial tension of water and water-hexadecane systems from 72 dynes/cm and 40 dynes/cm to 28 to 30 dynes/cm and 1 to 3 dynes/cm, respectively. These results were comparable with chemical surfactants with respect to surface tension reduction (Slic Gone 34 dynes/cm and Castrol 30 dynes/cm). The low interfacial tension allows the formation of stable emulsion. The two cultures were grown on different substrates, namely, glucose, mannitol, glycerol, hexadecane, oily sludge, and crude oil. Emulsion formation of hexadecane in water was tested with the cell-free broth containing biosurfactant from the respective substrate broths. Emulsions of 56 percent stability to 100 percent stability were obtained from these biosurfactant-containing broths. Both biosurfactants were able to emulsify crude oil. A surfactant's ability to form a stable emulsion is the first step in oil spill cleanup. The emulsified oil can then be acted upon very easily by the microorganisms under study. Therefore, the biosurfactants produced by the microorganisms under study offer a good potential for use in oil spill cleanup.

Isolation of Two Strains for Microbial Desulfurization of Dibenzothiophene

2013 ◽  
Vol 726-731 ◽  
pp. 3957-3960
Author(s):  
Pei Pei Han ◽  
Yong Wei Chen ◽  
Ping Zhe Jiang ◽  
Zhi Lei Tan ◽  
Shi Ru Jia
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Sequence Analysis ◽  
Crude Oil ◽  
16S Rdna ◽  
Sea Water ◽  
16S Rdna Sequence ◽  
Acinetobacter Radioresistens ◽  
16S Rdna Sequence Analysis ◽  
Maldi Biotyper ◽  
Microbial Desulfurization

Two desulfurizing strains S1 and S2 were isolated from sea water from Crude Oil Terminal at Tianjin Port. The ability of the two strains to convert dibenzothiophene (DBT) to 2-dihydroxybiphenyl (HBP) was tested by Gibbs assay, which indicated that 2-HBP existed in the metabolite. The strains S1 and S2 were identified by both 16S rDNA sequence analysis and MALDI BioTyper system as Agrobacterium tumefaciens and Acinetobacter radioresistens respectively, which could degrade 22.3% and 22.4% of DBT in 46 hours respectively.

scholarly journals Complete Genome Sequence of Hydrocarbon-Degrading Halotolerant Acinetobacter radioresistens DD78, Isolated from the Aconcagua River Mouth in Central Chile

2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Constanza C. Macaya ◽  
Valentina Méndez ◽  
Roberto E. Durán ◽  
Patricia Aguila-Torres ◽  
Francisco Salvà-Serra ◽  
...  
Keyword(s):  
Crude Oil ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
River Mouth ◽  
Polluted Soil ◽  
Central Chile ◽  
Coding Sequences ◽  
Content Type ◽  
Acinetobacter Radioresistens

Acinetobacter radioresistens strain DD78 (= CCUG 69565) is a soil hydrocarbon-degrading and biosurfactant-producing bacterium isolated from chronically crude oil-polluted soil of the Aconcagua River mouth in Chile. The 3.25-Mb A. radioresistens DD78 genome (41.8% GC content) was completely sequenced, with 4 replicons, 2,970 coding sequences, and 77 tRNAs.

Recovery of crude oil from oily sludge in an oilfield by sophorolipid

2019 ◽  
Vol 37 (13) ◽  
pp. 1582-1588 ◽  
Author(s):  
Xiaonan Liu ◽  
Tiantian Yao ◽  
Ruiqiu Lai ◽  
Jianlong Xiu ◽  
Lixin Huang ◽  
...  
Keyword(s):  
Crude Oil ◽  
Oily Sludge

scholarly journals Study on Reutilization of Pyrolytic Residues of Oily Sludge

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Chao Tang ◽  
Jiaojiao Guan ◽  
Shuixiang Xie
Keyword(s):  
Activated Carbon ◽  
Crude Oil ◽  
Pore Diameter ◽  
Chemical Separation ◽  
Pyrolytic Carbon ◽  
Oily Sludge ◽  
Average Pore ◽  
Adsorption Effect ◽  
Secondary Pollution ◽  
Better Than

Pyrolytic residues of oily sludge are a kind of hazardous solid waste produced by high-temperature pyrolysis of oily sludge, which still contains a certain amount of mineral oil; improper disposal can cause secondary pollution. In order to reutilize the pyrolytic residues of oily sludge, the pyrolytic carbon in pyrolytic residues is recovered by a combination of physical flotation and chemical separation, and they are used for the treatment of oilfield wastewater and adsorption of oil. The results showed that the purity of the pyrolytic carbon is 95.93%; many pores of different sizes are distributed on the surface, with mainly mesoporous distribution. Specific surface area, pore volume, and average pore diameter reach 454.47 m2/g, 0.61 cm3/g, and 6.91 nm, respectively. Adsorption effect of pyrolytic carbon on COD and oil in oilfield wastewater is better than that of activated carbon at the same condition. With regard to adsorption on diesel and crude oil, the initial instantaneous adsorption rate of pyrolytic carbon is 3.8 times and 1.86 times faster than that of activated carbon, respectively. When pyrolytic carbon reaches saturated adsorption, cumulative adsorption of activated carbon on diesel and crude oil is much lower than that of pyrolytic carbon.

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