Determination on a micro scale of concentration and specific radioactivity of inorganic phosphate ions in whole blood and packed red cells

1964 ◽  
Vol 16 (4) ◽  
pp. 457-464 ◽  
Author(s):  
B. Vestergaard-bogind
Keyword(s):  
Whole Blood ◽  
Inorganic Phosphate ◽  
Specific Radioactivity ◽  
Red Cells ◽  
Micro Scale ◽  
Phosphate Ions

The Long-Term Stability in Whole Blood of 14 Commonly-Requested Hormone Analytes

1994 ◽  
Vol 31 (6) ◽  
pp. 561-565 ◽  
Author(s):  
M J Diver ◽  
J G Hughes ◽  
J L Hutton ◽  
C R West ◽  
L J Hipkin
Keyword(s):  
Ambient Temperature ◽  
Whole Blood ◽  
Great Majority ◽  
Red Cells ◽  
Clinical Interpretation ◽  
Term Stability ◽  
Long Term Stability ◽  
Plasma Hormones

Concentrations of 14 commonly-requested plasma hormones were measured in octuplicate in each of six subjects to determine their stability when unseparated from red cells for periods up to 1 week. Most of the analytes were stable when stored in this way and although statistically significant changes were recorded, in the great majority of cases the changes seen would have no bearing on the clinical interpretation of the result. In the light of these findings, we would confidently report results of analyses for these hormones in plasma that had remained in contact with red cells at ambient temperature for long periods of time.

scholarly journals Centrifugation and capillarity integrated into a multiple analyte whole blood analyser

1995 ◽  
Vol 17 (3) ◽  
pp. 99-104 ◽  
Author(s):  
C. T. Schembri ◽  
T. L. Burd ◽  
A. R. Kopf-Sill ◽  
L. R. Shea ◽  
B. Braynin
Keyword(s):  
Whole Blood ◽  
Clinical Chemistry ◽  
Capillary Forces ◽  
Red Cells ◽  
Single Use ◽  
Absorbance Data

A unique clinical chemistry analyser is described which processes 90 μl of whole blood (fingerstick or venous) into multiple aliquots of diluted plasma and reports the results of 12 tests in 14 min. To perform a panel of tests, the operator applies the unmetered sample directly into a single use, 8 cm diameter plastic rotor which contains the required liquid diluent and dry reagents. Using centrifugal and capillary forces, the rotor meters the required amount of blood, separates the red cells, meters the plasma, meters the diluent, mixes the fluids, distributes the fluid to the reaction cuvettes and mixes the reagents and the diluted plasma in the cuvettes. The instrument monitors the reagent reactions simultaneously using nine wavelengths, calculates the results from the absorbance data, and reports the results.

scholarly journals The Increased Effectiveness of Platelet Concentrates Prepared in Acidified Plasma

Blood ◽  
1966 ◽  
Vol 27 (4) ◽  
pp. 449-459 ◽  
Author(s):  
FREDERICK A. FLATOW ◽  
EMIL J. FREIREICH
Keyword(s):  
Citric Acid ◽  
Acid Medium ◽  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
Red Cells ◽  
Platelet Concentrates ◽  
Standard Methods ◽  
Aggregation Of Platelets ◽  
Concentrate Preparation

Abstract Platelet concentrates prepared in acidified plasma (pH 6.5-6.7) are superior to concentrates prepared by standard methods, and are 80-90 per cent as effective as platelet rich plasma (PRP). The use of excess citric acid to acidify plasma promotes resuspension of the concentrate by eliminating clumping, which is a major factor in the decreased effectiveness of standard concentrates. Analysis of posttransfusion recovery and survival of platelets reveals no evidence of platelet injury in an acid medium. Acidification of PRP inhibits the aggregation of platelets by adenosine diphosphate (ADP). The presence of endogenous ADP may be an important factor in clumping during standard concentrate preparation. A method of acidification of PRP using citric acid is described which allows preparation of an effective concentrate from fresh whole blood without subjecting the red cells to acid pH. Reconstitution of the acidified platelet poor plasma and its native red cells increases the citrate molarity by less than 6 per cent and results in minimal decrease in pH of the whole blood.

A Method for Measurement of Sodium and Potassium in Erythrocytes and Whole Blood

1969 ◽  
Vol 15 (8) ◽  
pp. 712-719 ◽  
Author(s):  
Helen I Bugyi ◽  
Eugene Magnier ◽  
William Joseph ◽  
Gordon Frank
Keyword(s):  
Clinical Significance ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Red Cells ◽  
Disease States ◽  
Precision And Accuracy ◽  
Sodium And Potassium

Abstract The analysis of concentration of cations in erythrocytes and plasma, in theory, offers a means of identifying disease states in peripheral blood. Technical difficulties associated with cation analysis in erythrocytes and whole blood have resulted in a lack of precision and accuracy. This lack has increased the difficulties of using the analysis of blood in the diagnosis of disease. It is felt that the technic discussed in this paper with its increased precision, in combination with previously mentioned research in the diagnosis of disease, will increase the clinical significance of the analysis of red cells and whole blood for the diagnosis of disease states.

scholarly journals Brief Report: Vitamin B12 and Folate Activity in Normal Human Platelets

Blood ◽  
1968 ◽  
Vol 31 (2) ◽  
pp. 258-262 ◽  
Author(s):  
HARVEY J. WEISS ◽  
ALAN KELLY ◽  
VICTOR HERBERT
Keyword(s):  
Platelet Count ◽  
Vitamin B12 ◽  
Whole Blood ◽  
Human Platelets ◽  
Red Cells ◽  
Red Cell ◽  
Normal Human

Abstract The vitamin B12 and folate content of human platelets have been determined. The B12 concentration was sixfold that in red cells and one-sixth that in leukocytes. In normal whole blood, with a platelet count of 300,000 per cu. mm., the B12 activity contributed by platelets would be 6-21 pg. per ml. The contribution of platelets to the folate activity of normal whole blood averaged 0.4-1.7 ng. per ml. The folate activity in platelets was one-fifth that in an equal volume of red cells, but unlike red cell folate, was not increased by incubating platelet extracts with plasma.

Greater increase in cytokine concentration after salvage with filtered whole blood than with washed red cells, but no difference in postoperative hemoglobin recovery

Transfusion ◽  
1999 ◽  
Vol 39 (3) ◽  
pp. 271-276 ◽  
Author(s):  
Anders R. Åvail ◽  
Monica Hyllner ◽  
Jan Peter Bengtson ◽  
Lars Carlsson ◽  
Anders Bengtsson
Keyword(s):  
Whole Blood ◽  
Red Cells ◽  
Cytokine Concentration

scholarly journals Fetal hemoglobin in paroxysmal nocturnal hemoglobinuria (PNH): evidence for derivation of HbF-containing erythrocytes (F cells) from the PNH clone as well as from normal hemopoietic stem cell lines

Blood ◽  
1978 ◽  
Vol 52 (4) ◽  
pp. 740-749 ◽  
Author(s):  
T Papayannopoulou ◽  
W Rosse ◽  
G Stamatoyannopoulos
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Lines ◽  
Whole Blood ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Red Cells ◽  
Hemopoietic Stem Cell ◽  
Normal Cells ◽  
F Cells ◽  
Stem Cell Lines

Abstract The cellular distribution of HbF was studied in nine patients with paroxysmal nocturnal hemoglobinuria (PNH) by measuring the level of HbF and determining the number of HbF-containing red cells (F cells) in whole blood and in the population of normal cells obtained after immune lysis of the abnormal erythrocytes. The amounts of HbF and the F cell frequencies found in the normal red cells were strikingly similar to the values seen in whole blood. The observed frequencies of F cells in normal cells best fitted those expected under the assumption that the F cells arise equally from normal hemopoietic stem cells and from the stem cells with the PNH defect. Since PNH appears to be a clonal hemopoietic stem cell disorder, this evidence argues against a derivation of F cells from distinct pluripotent stem cell lines.

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