Comparison of ultracentrifugation and a precipitation method for high-density lipoprotein cholesterol quantitation in insulin-dependent diabetic patients

1989 ◽  
Vol 49 (6) ◽  
pp. 589-593 ◽  
Author(s):  
T. Jensen ◽  
S. Stender ◽  
M. Deckert
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Precipitation Method ◽  
Diabetic Patients ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

scholarly journals “STUDY OF TOTAL CHOLESTEROL (TC), TRIACYLGLYCEROLS (TG), HIGH DENSITY LIPOPROTEIN CHOLESTEROL (HDL-C) IN TYPE II DIABETES MELLITUS.”

2017 ◽  
Vol 10 (2) ◽  
pp. 116 ◽  
Author(s):  
Smita V Patil ◽  
Anita P Mandare ◽  
Gaikwad B Pandurang
Keyword(s):  
Diabetes Mellitus ◽  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Diabetic Patients ◽  
Type 2 Dm

Objective: There are probably 100 million people in the world with diabetes mellitus (DM), and incidences of diabetes are on the rise. Dyslipidemia is one of the common disorders which are seen in most of the diabetes patients, which causes cardiovascular disorders. The aim of this study is to investigate the total cholesterol (TC), triacylglycerols (TGs), high-density lipoprotein cholesterol (HDL-C) in Type 2 DM, and healthy controls.Methods: The study was conducted on 50 controls and 50 Type 2 diabetic subjects between age group of 30 and 60 years. Serum TC was determined by an enzymatic (cholesterol oxidase/phenol-aminophenazone [PAP]) colorimetric method and TGs were determined by an enzymatic (glycerol phosphate oxidase-PAP) method, and HDL-C was estimated by a precipitant method. Statistical analysis was done using unpaired t-test.Results: The mean value of TC, TGs, and HDL-C in normal subjects is 165.5±24.24, 118.7±41.58, 28.38±7.85 mg/dl, respectively, and the value of TC, TGs, and HDL-C in diabetic patients is 179±31.69, 164.35±27.93, 25.4±6.86 mg/dl, respectively. The observed difference in the means of TC, TGs, and HDL-C in normal and diabetic are statistically significant (p<0.05).Conclusion: From the present study, it is concluded that TC, TGs, and HDL-C levels for all persons with Type 2 DM should be done as a routine test. Furthermore, early diagnosis and treatment of dyslipidemia can be used as a preventive measure for the development of cardiovascular disease in Type 2 diabetes.Keywords: Diabetes mellitus, Dyslipidemia, Lipid profile, Total cholesterol, Triglycerides, High-density lipoprotein cholesterol.

Comparison of Intraperitoneal and Subcutaneous Insulin Administration on Insulin Sensitivity and Serum Lipids in Type I Diabetic Patients on Continuous Ambulatory Peritoneal Dialysis Treatment

1995 ◽  
Vol 88 (4) ◽  
pp. 427-432 ◽  
Author(s):  
Jorma T. Lahtela ◽  
Jukka Mustonen ◽  
Amos Pasternack
Keyword(s):  
Peritoneal Dialysis ◽  
Insulin Sensitivity ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Diabetic Patients ◽  
Subcutaneous Insulin ◽  
Intraperitoneal Insulin

1. The metabolic effects of intraperitoneal and subcutaneous insulin delivery were compared in a crossover manner in six C-peptide-negative diabetic patients with end-stage renal disease on continuous ambulatory peritoneal dialysis. Each treatment period lasted at least 3 months. Hyperinsulinaemic euglycaemic clamp was performed and glucose turnover assessed using [3-3H]glucose as a tracer. 2. During intraperitoneal delivery the daily insulin dose was 2.4 times higher than during subcutaneous administration and glycaemic control was significantly better (HbA1c 7.63% ± 0.46% and 9.52% ± 0.51% during intraperitoneal and subcutaneous insulin respectively, P < 0.01). The number of hypoglycaemic episodes was lower during intraperitoneal insulin than during subcutaneous therapy. 3. Intraperitoneal insulin resulted in an enhanced glucose disposal rate (P < 0.01) and reduced fasting hepatic glucose production (P < 0.01). High-density lipoprotein-cholesterol decreased and the ratio of low-density lipoprotein/high-density lipoprotein-cholesterol increased significantly (P < 0.05) during intraperitoneal insulin delivery. 4. The results suggest that intraperitoneal insulin, while resulting in better glycaemic control and improved insulin sensitivity than subcutaneous insulin, increases serum triacylglycerol and total cholesterol and reduces high-density lipoprotein-cholesterol, possibly via a direct effect on the liver.

scholarly journals Association of the Monocyte–to–High-Density Lipoprotein Cholesterol Ratio With Diabetic Retinopathy

2021 ◽  
Vol 8 ◽  
Author(s):  
Xixiang Tang ◽  
Ying Tan ◽  
Yi Yang ◽  
Mei Li ◽  
Xuemin He ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Macular Oedema ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Cross Sectional

Background: Chronic inflammation in type 2 diabetes mellitus (T2DM) is an essential contributor to the development of diabetic retinopathy (DR). The monocyte–to–high-density lipoprotein cholesterol (HDL-C) ratio (MHR) is a novel and simple measure related to inflammatory and oxidative stress status. However, little is known regarding the role of the MHR in evaluating the development of DR.Methods: A total of 771 patients with T2DM and 607 healthy controls were enrolled in this cross-sectional study. MHR determination and eye examination were performed. The association of MHR with the prevalence of DR in T2DM patients was analyzed.Results: The MHR in patients with DR was significantly higher than that in both non-DR diabetic patients (P &lt; 0.05) and healthy controls (P &lt; 0.01). No significance was observed in the MHR of different DR severity grades. Moreover, the MHR was similar between patients with non-macular oedema and those with macular oedema. Logistic regression analysis demonstrated that MHR was independently associated with the prevalence of DR in diabetic patients [odds ratio (OR) = 1.438, 95% confidence interval (CI): 1.249–1.655, P &lt; 0.01]. After additional stratification by HbA1c level and diabetic duration, the MHR was still independently associated with the prevalence of DR.Conclusions: Our study suggests that the MHR can be used as a marker to indicate the prevalence of DR in patients with T2DM.

A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol.

1981 ◽  
Vol 27 (1) ◽  
pp. 175-178 ◽  
Author(s):  
R B Schifman ◽  
P F Brumbaugh ◽  
D Grover ◽  
P R Finley ◽  
E J Harrow
Keyword(s):  
High Density Lipoprotein ◽  
Cellulose Acetate ◽  
High Density Lipoprotein Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Precipitation Method ◽  
Electrophoretic Method ◽  
Electrophoretic Procedure

Abstract We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV ws 4.1% (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0% (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9% at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically correlated well with its calculated concentrations obtained by the precipitation method. Measurement of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.

scholarly journals CORRELATION OF NON-HIGH-DENSITY LIPOPROTEIN CHOLESTEROL WITH LIPOPROTEIN(A) IN DIABETIC PATIENTS

2018 ◽  
Vol 11 (9) ◽  
pp. 499
Author(s):  
Vasanthan M
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
High Density ◽  
The Other ◽  
Lipoprotein Cholesterol ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Lipoprotein A ◽  
Artery Disease

Objective: The objective of this study is to estimate the level of lipoprotein(a) and lipid profile, especially non-high-density lipoprotein cholesterol (non-HDL-C) in diabetic patients, and to correlate the same with healthy controls (non-diabetics).Methods: A total of 30 non-diabetic subjects and 30 diabetic patients were included in the study. Lipoprotein(a) (Lp[a]) was estimated by immunoturbidimetry and the other parameters by their respective methods in biochemistry auto-analyzer.Result: From the study, it was found that both Lp(a) and non-HDLC were increased in diabetics when compared to the non-diabetic controls. It was also evident that there is a positive correlation of Lp(a) levels with non-HDL C.Conclusion: Lp(a) and non-HDL C are responsible for atherogenic events in uncontrolled diabetic patients, leading to risks such as coronary artery disease.

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