Implication of Smad2 and Smad3 in Transforming Growth Factor-β-induced Posterior Capsular Opacification of Human Lens Epithelial Cells

2014 ◽  
Vol 40 (4) ◽  
pp. 386-397 ◽  
Author(s):  
Hua Li ◽  
Xiaoyong Yuan ◽  
Jun Li ◽  
Xin Tang
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Human Lens ◽  
Posterior Capsular Opacification ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells

scholarly journals RNA Interference TargetingSnailInhibits the Transforming Growth Factorβ2-Induced Epithelial-Mesenchymal Transition in Human Lens Epithelial Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ping Li ◽  
Jiaona Jing ◽  
Jianyan Hu ◽  
Tiejun Li ◽  
Yuncheng Sun ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Transforming Growth Factor ◽  
Epithelial Mesenchymal Transition ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Epithelial Cell Line ◽  
Migration Ability ◽  
Mesenchymal Transition ◽  
Response Characteristics ◽  
Human Lens Epithelial Cells

Epithelial-msenchymal transition (EMT) contributes to posterior capsule opacification (PCO) type of cataract. Transcription factorsSnailis a key trigger of EMT activated by transforming growth factorβ(TGFβ). This study was done to investigate the effect ofSnailtargeting siRNA on TGFβ2-induced EMT in human lens epithelial cells. TGFβ2 treatment of cultured human epithelial cell line (HLEB3) upregulated the expression ofSnailand the EMT relevant molecules such as vimentin andα-SMA but downregulated the expression of keratin and E-cadherin. After the stimulation of TGFβ2, the HLEB3 cells became fibroblast-like in morphology, and the junctions of cell-cell disappeared. TGFβ2 treatment also enhanced migration ability of HLEB3 cells. TGFβ2-inducedSnailexpression and EMT were significantly inhibited bySnailsiRNA. By analyzing the response characteristics of HLEB3 in TGFβ2-induced EMT model with/withoutSnail-specific siRNA, we concluded thatSnailis an element in the EMT of HLEB3 cells induced by TGFβ2.SnailsiRNA targeting can block the induced EMT and therefore has the potential to suppress the development of PCO.

Characterization of Zinc Oxide Nanoparticles and their Inhibitory Effect on Human Lens Epithelial Cells under UVB Irradiation

2013 ◽  
Vol 788 ◽  
pp. 61-64
Author(s):  
Da Dong Guo ◽  
Hong Ying Tang ◽  
Hong Sheng Bi
Keyword(s):  
Epithelial Cells ◽  
Zno Nanoparticles ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Posterior Capsular Opacification ◽  
Lens Epithelial Cells ◽  
Epithelial Cell Proliferation ◽  
Uvb Irradiation ◽  
Human Lens Epithelial Cell ◽  
Human Lens Epithelial Cells

Posterior capsular opacification is the most frequent complication after cataract surgery, which is induced by the proliferation, differentiation and migration of lens epithelial cells. Inhibiting the proliferation of human lens epithelial cells could reduce the occurrence of posterior capsular opacification. To explore the possible potential of ZnO nanoparticles in treating posterior capsular opacification, we investigated the effect of ZnO nanoparticles on the human lens epithelial cell proliferation in the presence and absence of ultraviolet B (UVB) irradiation via 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The results indicate that ZnO nanoparticles could apparently inhibit the proliferation of human lens epithelial cells in a dose-dependent manner, and UVB irradiation can further enhance this inhibitory role for the human lens epithelial cell proliferation, indicating the great potential of ZnO nanoparticles in treating posterior capsular opacification in clinical application.

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