Giemsa Staining of Autoradiograms Prepared with Stripping Film

1955 ◽  
Vol 30 (4) ◽  
pp. 161-162 ◽  
Author(s):  
William D. Gude ◽  
Arthur C. Upton ◽  
T. T. Odell
Keyword(s):  
1975 ◽  
Vol 29 (1) ◽  
pp. 41-59 ◽  
Author(s):  
K. -H. Grzeschik ◽  
My. A. Kim ◽  
Renate Johannsmann

Author(s):  
Fahimeh Asadi-Amoli ◽  
Zohreh Nozarian ◽  
Vahid Mehrtash ◽  
Hooshang Beheshtnejad ◽  
Avishan Shabani

2000 ◽  
Vol 38 (3) ◽  
pp. 1131-1135 ◽  
Author(s):  
D. De Groote ◽  
R. Ducatelle ◽  
L. J. van Doorn ◽  
K. Tilmant ◽  
A. Verschuuren ◽  
...  

Recently, a new 16S ribosomal DNA-based PCR assay was developed for the specific detection of “Candidatus Helicobacter suis” (former “Gastrospirillum suis”) in porcine gastric samples. In the present study, this PCR assay was compared to three other invasive diagnostic methods (rapid urease test, immunohistochemistry, histologic analysis by Giemsa staining). Antral stomach samples from 200 slaughterhouse pigs from Belgium and The Netherlands were examined. Bacterial presence was determined in 77% (154 of 200) of the samples by PCR in combination with Southern blot hybridization, 56% (111 of 200) of the samples by immunohistochemistry, 61% (122 of 200) of the samples by urease testing (20 h postinoculation [p.i.]), 36% (71 of 200) of the samples by urease testing (3 h p.i.), and 33% (65 of 200) of the samples by Giemsa staining. The intrinsic specificity of the PCR assay was assessed by Southern blot analysis with an “Candidatus H. suis”-specific probe and sequencing of PCR products. Interassay sensitivity and specificity values were assessed for each test by pairwise comparisons between tests. Agreement between tests was evaluated by calculating Cohen's kappa coefficient. From that analysis, the PCR assay was considered the most reliable benchmark. Microscopic detection of immunohistochemically labeled or Giemsa-stained “Candidatus H. suis” cells in stomach sections proved to be highly specific (100%) but relatively insensitive (72 and 42%, respectively) compared to the PCR assay. A longer incubation time of the urease test improved its sensitivity considerably (74 versus 55%) but was accompanied by a loss of specificity (72 versus 93%). In conclusion, we found the “Candidatus H. suis”-specific PCR assay to be a sensitive and reliable diagnostic method for the detection of “Candidatus H. suis” in the stomachs of pigs and could prove to be a valuable tool for further epidemiological studies both for “Candidatus H. suis”- and for “Helicobacter heilmannii” type 1-related research.


Author(s):  
Hirofumi Takano ◽  
Megumi Yasunaga ◽  
Ieharu Yamazaki ◽  
Masako Nishikawa ◽  
Yosuke Masamoto ◽  
...  

Author(s):  
C. Cagri Cingi ◽  
Deniz Yeni ◽  
Ebubekir Yazici ◽  
Tugba Akbas Cine ◽  
Mehmet Ucar

Transmissible Venereal Tumor (TVT) is one of the most uncontrolled spreading tumor via mating between dogs observed in both sexes. The clinical findings are less remarkable and are usually located in the caudal part of the penis and may include preputial discharge, licking of the region, dysuria, phimosis or paraphimosis (occasionally). In this study, the incidence of TVT and the relations of tumor with some factors were investigated in 145 male dogs, in Eskiºehir, Turkey. TVT’s diagnosis was based on location of the tumor mass and mainly exfoliative cytological findings. Smears of caudal part of the penis were painted with Giemsa staining method. The cells in the smears were identified as typical transmissible venereal tumor cells, polymorphnuclear leukocytes (PMN), erythrocyte, parabasal, intermediate, nucleated and anuclear superficial cells. In exfoliative cytological examinations, TVT cells were observed in the smears of 17 dogs (11,72 %), but only four of them (2,76 %) had TVT lesions clinically. Thirteen (8,97 %) of dogs had TVT cells but not having TVT lesions . It was found that the ages, weights and breeds of dogs had no effect on the TVT lesions and TVT cells. TVT positive animals were determined to have more erythrocyte and intermediate density than negatives and no association with other cells were found. PMNs were significantly different and high in the TVT cell positive cases compared to the negatives. In dogs with negative TVT cells, parabasal, intermediate, nuclear/anuclear superficial cells were more than those positives. As a result, the male dogs having no lesions could be infected with TVT. Dogs should be examined not only clinically, but also cytologically, to determine whether TVT cells are present. This method can be an easy way to find and treat TVT lesion-free but infected dogs at an early time.


2020 ◽  
Vol 3 (1) ◽  
pp. 1-6
Author(s):  
Rini Syahrani Harahap ◽  
Delyuzar ◽  
Jessy Chrestella

Background: Helicobacter pylori is a well known pathogen which is recognized as the most frequent infection of individuals. Helicobacter pylori plays important role in the etiology og gastritis, gastric and duodenal ulcers. We investigated the presence of Helicobacter pylori as an extragastric reservoir in the tonsilectomy specimens. Aim: To determine the presence of Helicobacter pylori in cases of chronic tonsillitis in tonsilectomy specimens. Methods: This is a descriptive study with cross sectional which the sample will be obtained using consecutive sampling method. We collect 43 cases with chronic tonsilitis were examined in tonsillectomy specimens for which available tissue for routin staining could be retrieved were histopathology chronic tonsillitis. The colonization of the helicobacter pylori have been evaluated with hematoxylin & Eosin and Giemsa stains under the light microscope. Results: With H&E staining, Helicobacter pylori has been detected in 23 cases (53,48%) of the tonsil-lectomy specimens in total. No colonization has been observed in the remaining 20 cases (46,51%). With giemsa staining Helicobacter pylori has been detected in 29 cases (67,74%) of the tonsillectomy specimens in total. No colonization has been observed in the remaining 14 cases (32,55%).   Keywords: Helicobacter pylori, Chronic Tonsillitis, H&E, Giemsa.


1985 ◽  
Vol 27 (3) ◽  
pp. 365-369 ◽  
Author(s):  
April Romagnano ◽  
W. Allan King ◽  
Claude-Lise Richer ◽  
Marie-Antoinette Perrone

A technique is described for the preparation of banded chromosomes from early equine embryos cultured for less than 10 h in a medium containing bromodeoxyuridine. In addition to standard Giemsa staining and C-banding, chromosomes thus prepared can also be R-banded by either the RBA or the RB-FPG methods. This technique is rapid, repeatable, and limits cell loss, making it ideal for the preparation of early embryos.Key words: embryos, chromosomes, banding, horse, cow.


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