Protective effect of Homer 1a against hydrogen peroxide-induced oxidative stress in PC12 cells

Abstract Objectives: Using a PC12 cell model with Hydrogen peroxide, we investigated the neuronal apoptotic gene expression and neuronal apoptosis after oxidative stress damage. We further explored protective effect of pioglitazone and its mechanisms. Methods: Taking H2O2 treated PC12 cells as oxidative stress damaged neuron models, MTT and flow cytometry methods were performed to measure the influence of H2O2 on neuronal apoptosis and the protective effect of pioglitazone. Neuronal apoptosis was detected by TUNEL staining. Real-time PCR and Western blot methods were performed to investigate the expression of PPARγ, Bax, Bcl-2 and Caspase-3. Results: H2O2 can induce the apoptosis of PC12 cells in an dose- and time-dependent manner. And H2O2 (100μmol/L, 24h) can induce the expression of PPARγ mRNA and protein (p<0.01). Pioglitazone significantly up-regulated the protein expression of Bax, caspase-3(p<0.01) and decreased the expression of Bcl-2(p<0.01). Pioglitazone can dose-dependently decrease the apoptotic ratio of H2O2-damaged PC12 cells. 1.0×10-6 mol/L pioglitazone can induce PPARγ mRNA and protein expression. Pioglitazone decreased Bax, caspase-3 protein expression(p<0.01) and increased Bcl-2 protein expression(p<0.01), thus down-regulated the expression ratio of Bax/Bcl-2(p<0.01) and decreased the apoptotic ratio of PC12 cells(p<0.01). GW9662, the antagonist of PPARγ, and PPARγ siRNA can offset the protective effect of pioglitazone on PC12 cells to different degrees(p<0.01). Conclusions: hydrogen peroxide can induce apoptosis of PC12 cells in dose- and time-dependent manner. PPARγ activation by pioglitazone can significantly decreased expression of Bax/Bcl-2 and Caspase-3, thus plays a part in neuron protective effects on H2O2-treated PC12 cells. The antagonist and RNAi of PPARγ can offset protective effect of pioglitazone to different degree, which indicates PPARγ activation exerts protective role in decreasing the apoptosis of PC12 cells.

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Protective effect of vanillic acid in hydrogen peroxide-induced oxidative stress in D.Mel-2 cell line

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2020.12.023 ◽

2020 ◽

Author(s):

Shagufta Taqvi ◽

Eijaz Ahmed Bhat ◽

Nasreena Sajjad ◽

Jamal S.M. Sabir ◽

Aleem Qureshi ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Protective Effect ◽

Cell Line ◽

Vanillic Acid

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Protective effect of CTRP6 on cerebral ischemia/reperfusion injury by attenuating inflammation, oxidative stress and apoptosis in PC12 cells

Molecular Medicine Reports ◽

10.3892/mmr.2020.11108 ◽

2020 ◽

Vol 22 (1) ◽

pp. 344-352

Author(s):

Ying Li ◽

Jie Sun ◽

Lei Gu ◽

Xufang Gao

Keyword(s):

Oxidative Stress ◽

Cerebral Ischemia ◽

Protective Effect ◽

Reperfusion Injury ◽

Pc12 Cells ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Cerebral Ischemia Reperfusion ◽

Cerebral Ischemia Reperfusion Injury

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Caffeic Acid - Potential Antioxidant in Cultured Human Retinal Pigment Epithelial Cells

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:4010 ◽

1970 ◽

Vol 66 (2) ◽

Author(s):

Dumitriţa RUGINǍ ◽

Adela PINTEA ◽

Raluca PÂRLOG ◽

Andreea VARGA

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Hydrogen Peroxide ◽

Protective Effect ◽

Caffeic Acid ◽

Reactive Oxygen ◽

Oxygen Species ◽

Antioxidant Defence ◽

Rpe Cells ◽

In Cells

Oxidative stress causes biological changes responsible for carcinogenesis and aging in human cells. The retinal pigmented epithelium is continuously exposed to oxidative stress. Therefore reactive oxygen species (ROS) and products of lipid peroxidation accumulate in RPE. Neutralization of ROS occurs in retina by the action of antioxidant defence systems. In the present study, the protective effect of caffeic acid (3,4-dihydroxy cinnamic acid), a dietary phenolic compound, has been examined in normal and in oxidative stress conditions (500 µM peroxide oxygen) in cultures human epithelial pigment retinal cells (Nowak, M. et al.). The cell viability, the antioxidant enzymes activity (CAT, GPx, SOD) and the level of intracellular reactive oxygen species (ROS) were determined. Exposure to l00 µM caffeic acid for 24 h induced cellular changes indicating the protective effect of caffeic acid in RPE cells. Caffeic acid did not show any cytotoxic effect at concentrations lower than 200 μM in culture medium. Treatment of RPE cells with caffeic acid causes an increase of catalase, glutathione peroxidase and superoxide dismutase activity, especially in cells treated with hydrogen peroxide. Caffeic acid causes a decrease of ROS level in cells treated with hydrogen peroxide. This study proved that caffeic acid or food that contain high levels of this phenolic acid may have beneficial effects in prevention of retinal diseases associated with oxidative stress by improving antioxidant defence systems.

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The Protective Effect of Docosahexaenoic Acid on PC12 Cells in Oxidative Stress Induced by H2O2 through the TrkB-Erk1/2-CREB Pathway

ACS Chemical Neuroscience ◽

10.1021/acschemneuro.1c00421 ◽

2021 ◽

Author(s):

Nana Bie ◽

Xiaojuan Feng ◽

Chenjing Li ◽

Meng Meng ◽

Chunling Wang

Keyword(s):

Oxidative Stress ◽

Docosahexaenoic Acid ◽

Protective Effect ◽

Pc12 Cells ◽

Creb Pathway

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Tamarix articulata Extracts Exhibit Antioxidant Activity and Offer Protection against Hydrogen Peroxide-Mediated Toxicity to Human Skin Fibroblasts

Journal of Toxicology ◽

10.1155/2020/8896263 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Abdullah M. Alnuqaydan

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Protective Effect ◽

Mtt Assay ◽

Skin Fibroblasts ◽

Time Dependent ◽

Significant Protection ◽

Methanolic Extracts ◽

Higher Doses ◽

Oxidative Insult

Tamarix articulata (TA) is a wild halophytic plant growing in extremely harsh environmental conditions in the deserts of Saudi Arabia. Evaluating the protective effect of the methanolic extract of different parts (fresh and dry leaves, stem, and root) of TA was determined by MTT assay using Hs27 skin fibroblasts as the cellular model. The study was designed and conducted in two sets. The first set assesses the toxicity profile of TA extracts in both concentration- and time-dependent ways on Hs27 cells. Our MTT results showed that methanolic extracts from all four parts of TA at varying doses (27.5, 55, 110, and 220 μg/mL) display negligible toxicity when exposed for 4 h. However, exposure of Hs27 cells to varying doses of all four TA extracts for 24 and 48 h promotes significant 23%, 24%, 26%, and 25% p < 0.05 and 35%, 36%, 39%, and 41% p < 0.05 cell toxicity at 220 μg/mL of all four TA extracts compared to untreated control cells. To evaluate the protection offered by TA extracts against H₂O₂, we perform a second set of experiments to preincubate Hs27 cells with the TA extracts in both dose- and time-dependent way. This is followed by 300 μM hydrogen peroxide- (H₂O₂-) mediated oxidative insult for 1 h. Using MTT assay, we found that methanolic extracts of TA at different time points (4, 24, and 48 h) and higher doses (220 μg/mL) provide significant protection in cell viability when challenged with H2O2-induced oxidative stress in Hs27 cells. The protective effect was more pronounced at 48 h and 220 μg/mL and the amounts were 39%, 41%, 41%, and 44% for stem, root, fresh leaf, and dry leaf TA extracts p < 0.05 , respectively, compared to untreated cells (2–4%). Collectively, the current study demonstrates that methanolic extracts of TA contain potential bioactive compounds and offer significant protection against H2O2-mediated oxidative stress in Hs27 skin fibroblasts.

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