Cytokine modulation (IL-6, IL-8, IL-10) by human breast milk lipids on intestinal epithelial cells (Caco-2)

2015 ◽  
pp. 1-8 ◽  
Author(s):  
Girolamo J. Barrera ◽  
Gabriela Sánchez
Keyword(s):  
Breast Milk ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Human Breast Milk ◽  
Intestinal Epithelial ◽  
Human Breast ◽  
Cytokine Modulation ◽  
Milk Lipids

scholarly journals Human breast milk-derived exosomes attenuate cell death in intestinal epithelial cells

2018 ◽  
Vol 24 (5) ◽  
pp. 278-284 ◽  
Author(s):  
Colin Martin ◽  
Mikita Patel ◽  
Sparkle Williams ◽  
Hamish Arora ◽  
Brian Sims
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Breast Milk ◽  
Epithelial Cells ◽  
Protective Effect ◽  
Cell Viability ◽  
Intestinal Epithelial Cells ◽  
Human Breast Milk ◽  
Intestinal Epithelial ◽  
Human Breast

Human breast milk has been shown to reduce the incidence of necrotizing enterocolitis (NEC). Breast milk has many components (immunoglobulins, proteins, fat, and, of recent interest, exosomes), but the specific component that affords protection against NEC is not known. Exosomes are small-nanometer vesicles that are rich in protein, lipid, and microRNA. Here, we hypothesized that human breast milk-derived exosomes can protect intestinal epithelial cells (IECs) from cell death. Human breast milk was collected, separated using ultracentrifugation, and quantified using NanoSight tracking analysis. Purified exosomes were added to IECs that had been treated with varying concentrations of H2O2. Cells were then incubated overnight with the human breast milk-derived exosomes and assessed for cell viability. Western blot analysis showed that both clathrin and CD81 were present in the purified sample. Oxidative stress using H2O2 caused a 50% decrease in cell viability and human breast milk-derived exosomes had a protective effect in IECs. In the presence of H2O2, exosomes had a statistically significant protective effect. The protection seen by human breast milk-derived exosomes was not attenuated by cycloheximide. Thus, human breast milk-derived exosomes allow IECs to be protected from oxidative stress, but the mechanism is still not clear. Exosomes derived from human breast milk are an attractive treatment concept for children with intestinal injury.

scholarly journals Temporal Changes of Human Breast Milk Lipids of Chinese Mothers

Nutrients ◽  
2016 ◽  
Vol 8 (11) ◽  
pp. 715 ◽  
Author(s):  
Francesca Giuffrida ◽  
Cristina Cruz-Hernandez ◽  
Emmanuelle Bertschy ◽  
Patric Fontannaz ◽  
Isabelle Masserey Elmelegy ◽  
...  
Keyword(s):  
Breast Milk ◽  
Temporal Changes ◽  
Human Breast Milk ◽  
Human Breast ◽  
Chinese Mothers ◽  
Milk Lipids

scholarly journals Effect of 9,12-Octadecadiynoic Acid on Neurobehavioral Development in Caenorhabditis elegans

2021 ◽  
Vol 22 (16) ◽  
pp. 8917
Author(s):  
Tun-Chieh Chen ◽  
How-Ran Chao ◽  
Ching-Ying Wu ◽  
Yun-Ru Lai ◽  
Chu-Huang Chen ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Breast Milk ◽  
Biological Function ◽  
Behavioral Development ◽  
Human Breast Milk ◽  
Related Gene ◽  
Human Breast ◽  
Neurobehavioral Development ◽  
Foraging Ability ◽  
Milk Lipids

Human breast milk lipids have major beneficial effects: they promote infant early brain development, growth and health. To identify the relationship between human breast milk lipids and infant neurodevelopment, multivariate analyses that combined lipidomics and psychological Bayley-III scales evaluation were utilized. We identified that 9,12-octadecadiynoic acid has a significantly positive correlation with infant adaptive behavioral development, which is a crucial neurodevelopment to manage risk from environmental stress. To further clarify the biological function of 9,12-octadecadiynoic acid in regulating neurodevelopment, Caenorhabditis elegans (C. elegans) was used as a model to investigate the effect of 9,12-octadecadiynoic acid on neurobehavioral development. Supplementation with 9,12-octadecadiynoic acid from the L1 to L4 stage in larvae affected locomotive behaviors and foraging ability that were not socially interactive, implying that 9,12-octadecadiynoic acid is involved in regulating the serotonergic neuronal ability. We found that supplementary 0.1 μM 9,12-octadecadiynoic acid accelerated the locomotive ability and foraging ability via increasing the expression of serotonin transporter mod-1. Antioxidant defense genes, sod-1, sod-3 and cyp-35A2 are involved in 9,12-octadecadiynoic acid-induced motor neuronal activity. Nevertheless, supplementary 9,12-octadecadiynoic acid at concentrations above 1 μM significantly attenuated locomotive behaviors, foraging ability, serotonin synthesis, serotonin-related gene expressions and stress-related gene expression, resulting in the decreased longevity of worms in the experiment. In conclusion, our study demonstrates the biological function of 9,12-octadecadiynoic acid in governing adaptive behavioral development.

scholarly journals Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus

Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 950 ◽  
Author(s):  
Hubert ◽  
Chiche ◽  
Legros ◽  
Jeannin ◽  
Montange ◽  
...  
Keyword(s):  
Breast Milk ◽  
Epithelial Cells ◽  
Zika Virus ◽  
Mammary Epithelial Cells ◽  
Mammary Glands ◽  
Mammary Epithelial ◽  
Human Breast Milk ◽  
Human Breast ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial

Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions of ZIKV have been notified, either following sexual intercourse or from the mother to fetus during pregnancy. Importantly, high viral loads have been detected in the human breast milk of infected mothers, and the existence of breastfeeding as a new mode of mother-to-child transmission of ZIKV was recently hypothesized. However, the maternal origin of infectious particles in breast milk is currently unknown. Here, we show that ZIKV disseminates to the mammary glands of infected mice after both systemic and local exposure with differential kinetics. Ex vivo, we demonstrate that primary human mammary epithelial cells were sensitive and permissive to ZIKV infection in this study. Moreover, by using in vitro models, we prove that mammary luminal- and myoepithelial-phenotype cell lines are both able to produce important virus progeny after ZIKV exposure. Our data suggest that the dissemination of ZIKV to the mammary glands and subsequent infection of the mammary epithelium could be one mechanism of viral excretion in human breast milk.

Effect of pasteurization on banked breast milk: Enhanced growth of intestinal epithelial cells

1995 ◽  
Vol 108 (4) ◽  
pp. A754 ◽  
Author(s):  
T.E. Smith ◽  
S. Hooy ◽  
M.T. Asquith ◽  
R. Emerson ◽  
B. Purtic ◽  
...  
Keyword(s):  
Breast Milk ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial
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