Quality changes of frozen Longissimus dorsi and Semimembranosus muscles and perirenal adipose tissue during storage in lambs fed dihydroquercetin or dry distilled rose petals supplemented diet.

Abstract The aim of the study was to determine the quality changes of frozen muscles of Longissimus dorsi and Semimembranosus and perirenal adipose tissue from lambs fed a diet supplemented by 7.5 mg dihydroquercetin/kg/d, or 545 mg dry distilled rose petals (DDRP)/kg/d. Three groups of 10 male lambs aged 65 days were fed 50 days ad libitum: a control (ground alfalfa + granular compound feed) and two experimental groups (the same diet but with the addition of phytonutrients). Samples were collected 1 d post mortem. A half of them were analyzed immediately and another one was vacuum-packed, frozen at -40°C and stored for 365 d at -18°C. It has been found that supplementing the lamb's diet with polyphenol-rich phytonutrients contributes to certain reduction in aerobic plate count, α-aminoacidic nitrogen, protein carbonyls, TBARS and the content of saturated fatty acids, but with small amplitudes, more pronounced in the m. Longissimus dorsi from lambs fed with 545 mg DDRP/kg/d. Further studies are needed to answer the question of whether the use of higher doses of DDRP or dihydroquercetin as supplements in the small ruminant's diet will be able to provide a more pronounced inhibitory effect on oxidation processes in frozen lamb.

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The effect of dietary dry distilled rose petals or dihydroquercetin supplementation on the oxidative stability and quality of lamb muscles and fat.

10.31220/agrirxiv.2021.00035 ◽

2021 ◽

Author(s):

Desislava B. Vlahova-Vangelova ◽

Desislav K. Balev ◽

Nikolay D. Kolev ◽

Margarita N. Terziyska ◽

Stefan G. Dragoev

Keyword(s):

Color Difference ◽

Monounsaturated Fatty Acids ◽

Inhibitory Effect ◽

Plate Count ◽

Longissimus Dorsi ◽

Control Group ◽

Rose Petals ◽

Aerobic Plate Count ◽

Yeasts And Molds

Abstract The objective of this study was to determine the changes in the quality of m. Longissimus dorsi and m. Semimembranosus and perirenal adipose tissue from lambs fed a diet supplemented by 7.5 mg dihydroquercetin/kg/day or 545 mg dry distilled rose petals (DDRP)/kg/d. The experiments were performed with 30 male lambs aged 65 days. They were divided into three groups of 10 animals fed 50 days ad libitum: control group (with ground alfalfa + granulated compound feed) and two experimental ones (with the same diet + phytonutrients). Samples stored for 1 and 7 days at 2 ± 2°C were examined. After 7 d of storage, the addition of 545 mg DDRP/kg/d contributed to a decrease in pH by 2-6% in both types of tissues and reduced the accumulation of α-aminoacidic nitrogen by 7-14% and TBARS by more than 20% in both muscles tested. The lambs' diet supplementation with both phytonutrients led to a reduction in the aerobic plate count and total combined yeasts and molds count. The addition of dihydroquercetin was found to be responsible for the reduction of FFA and the total color difference as well as for approx. a 5% increasing the amount of monounsaturated fatty acids. This influence is more pronounced in m. Longissimus dorsi. Further studies are needed to determine whether the application of higher doses of DDRP and dihydroquercetin as dietary supplements in lambs will provoke a more pronounced inhibitory effect on lipid oxidation and the fatty acid composition of lamb and fats.

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The Effect of Dietary Dry Distilled Rose Petals or Dihydroquercetin Supplementation on the Oxidative Stability and Quality of Lamb Muscles and Fat

Letters in Applied NanoBioScience ◽

10.33263/lianbs114.42804293 ◽

2021 ◽

Vol 11 (4) ◽

pp. 4280-4293

Keyword(s):

Oxidative Stability ◽

Color Difference ◽

Plate Count ◽

Longissimus Dorsi ◽

Control Group ◽

Rose Petals ◽

Aerobic Plate Count ◽

Yeasts And Molds ◽

Higher Doses

The study's objective was to determine the changes in oxidative stability of m. Longissimus dorsi, m. Semimembranosus and perirenal adipose tissue from lambs fed a diet supplemented by 7.5 mg dihydroquercetin/kg life wight/d or 545 mg dry distilled rose petals (DDRP)/kg life wight/d.The experiments were performed with 30 male lambs aged 65 days. They were divided into three groups of 10 animals fed 50 days ad libitum: the control group (with ground alfalfa + granulated compound feed) and two experimental ones (with the same diet + phytonutrients). Samples stored 7 days at 0-4°C were examined. After 7 d of storage in both muscles, the addition of 545 mg DDRP/kg life wight/d contributed to the reduction of α-aminoacidic nitrogen with approximately 0.5 mg Leu/g and the FFA with 0.5-2%. The addition of 7.5 mg dihydroquercetin contributed to the reduction of TBARS with 0.14-0.21 mg MDA/kg, the total color difference, and a slight increase in the share of the MUFA more pronounced in m. Longissimus dorsi. The reduction of aerobic plate count and total yeasts and molds count were determined too. Further studies with higher doses of phytonutrient’s supplementation are needed to determine if it will provoke a more pronounced oxidative stability.

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Quality changes and shelf life prediction of cooked cured ham stored at different temperatures

Journal of Food Protection ◽

10.4315/jfp-20-374 ◽

2021 ◽

Author(s):

Miao Ran ◽

Laping He ◽

Cuiqin Li ◽

Qiujin Zhu ◽

Xuefeng Zeng

Keyword(s):

Shelf Life ◽

Life Prediction ◽

Storage Temperature ◽

Back Propagation ◽

Plate Count ◽

Quality Changes ◽

Ann Model ◽

Shelf Life Prediction ◽

Different Temperatures ◽

Aerobic Plate Count

Cooked cured ham is a ready-to-eat food that is popular among consumers. Stored temperature has a key effect on the quality and shelf life of ham. In this work, the quality changes and shelf life prediction of cooked cured ham stored at different temperatures were investigated. Sensory evaluation, physical and chemical indicators, and aerobic plate count were determined. Results showed that high storage temperature of cooked ham accelerates quality deterioration. Partial least squares (PLS) regression analysis based on the variable importance for projection (VIP) identified nine important variables for predicting the shelf life of cooked cured ham. Compared with either PLS or back-propagation artificial neural network (BP-ANN), the hybrid PLS–BP-ANN model better predicts the shelf life of cooked cured ham using the nine important variables. This study provides a theoretical basis and data support for the quality control of cooked cured ham and a new idea for research on the shelf life prediction of cooked cured ham.

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Effects of Fish-Derived Biological Preservatives on Cold Storage of Grass Carp (Ctenopharyngodon idellus) Fillets

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-571 ◽

2016 ◽

Vol 79 (10) ◽

pp. 1707-1716 ◽

Cited By ~ 6

Author(s):

TINGTING LI ◽

YANG JIANG ◽

GAOWEI JIN ◽

QIANCHENG ZHAO ◽

JIANRONG LI

Keyword(s):

Grass Carp ◽

Water Distribution ◽

Inhibitory Effect ◽

Plate Count ◽

K Value ◽

Antimicrobial Substances ◽

Distribution Parameters ◽

Epidermal Mucus ◽

Aerobic Plate Count ◽

Clarias Fuscus

ABSTRACT Two kinds of fish-derived natural antimicrobial substances, protamine and catfish (Clarias fuscus) epidermal mucus extract (CEME), were evaluated for their effect on the quality of grass carp fillets. Fillets were atmosphere packaged in cast polypropylene bags and were stored at 4°C in a low-temperature incubator without light. Solutions (0.5%, wt/vol) of CEME and protamine were used for dip pretreatment, respectively. Bacteriological (aerobic plate count, Shewanella putrefaciens, Pseudomonas fluorescens) and physicochemical (pH, total volatile basic nitrogen, K value, surface color, texture profiles, water distribution) parameters and volatile odor compounds were measured on days 0, 3, 6, 9, 12, and 15. The results indicated that CEME and protamine could improve all bacteriological and physicochemical indexes, except water distribution within muscle. In comparison, CEME had a more inhibitory effect, as determined by the aerobic plate count, and was more effective in inhibiting the growth of P. fluorescens; however, protamine was more effective in preventing the production of sulfur-organic (H2S) group substances. Protamine was more effective in inhibiting the growth of S. putrefaciens. This research suggests that protamine and CEME have the potential to improve the quality and extend the shelf life of grass carp fillets.

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The effect of food intake and dietary fatty acids on the activity of stearoyl-CoA Δ9 desaturase in pig adipose tissue

The Journal of Agricultural Science ◽

10.1017/s0021859600063474 ◽

1986 ◽

Vol 106 (3) ◽

pp. 601-609 ◽

Cited By ~ 10

Author(s):

K. J. Buller ◽

M. Enser

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Linoleic Acid ◽

Food Intake ◽

Desaturase Activity ◽

Corn Oil ◽

Saturated Fatty Acids ◽

Dietary Fatty Acids ◽

Perirenal Adipose Tissue ◽

Ad Libitum

SummaryStearoyl-CoA desaturase activity was determined in the subcutaneous and perirenal adipose tissue of pigs either fed ad libitum, or starved for up to 48 h. The effect of saturated fatty acids, as suet, or linoleic acid, as corn oil, on the enzyme activity was determined in pigs refed after starvation or fed 50% of the ad libitum intake.Desaturase activity was similar in the outer and inner backfat and was twice as high as in perirenal adipose tissue. Starvation for 48 h decreased the activity by 70, 91 and 94 % in the outer and inner backfat and perirenal adipose tissue respectively. Lowering food intake to 50 % of normal decreased desaturase activity by 46% in the outer back-fat. There was no significant difference in the response of desaturase to 10% corn oil or suet added to the diet during refeeding after starvation or to the diet of underfed pigs. Although the enzyme thus appeared insensitive to dietary saturated fatty acids or linoleic acid, it was inhibited by linoleoyl-CoA in vitro.

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Fatty acid composition of meat and adipose tissue from Krškopolje pigs and commercial fatteners in Slovenia

Archives Animal Breeding ◽

10.5194/aab-53-73-2010 ◽

2010 ◽

Vol 53 (1) ◽

pp. 73-84 ◽

Cited By ~ 5

Author(s):

M. Furman ◽

Š. Malovrh ◽

A. Levart ◽

M. Kovač

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Subcutaneous Adipose Tissue ◽

Saturated Fatty Acids ◽

Longissimus Dorsi ◽

Pufa Ratio ◽

Subcutaneous Adipose

Abstract. The fatty acid composition of meat and subcutaneous adipose tissue of the indigenous Slovenian pig breed (Krškopolje, KP), which is raised extensively, was compared with that of commercial fatteners (CP) from intensive farms. Commercial fatteners were divided into three groups according to lean meat percentage: meaty, normal and fatty. The m. longissimus dorsi of Krškopolje pigs contained less C18:0 fatty acids than that of commercial fatteners and less C16:0 than that of the fatty group. The proportions of long chain fatty acids C20:4 n-6, C20:5 n-3 and C22:5 n-3 in the Krškopolje pigs and fatty groups were also significantly different. The highest proportion of essential C18:2 n-6 and C18:3 n-3 fatty acids were found in the meaty and normal groups. Intramuscular fat content of the m. longissimus dorsi did not differ between the Krškopolje pigs, and the fatty and normal groups. The Krškopolje pigs had the lowest proportion of saturated fatty acids (SFAs), while both the Krškopolje pigs and the fatty group have a lower proportion of polysaturated fatty acids (PUFAs) than the meaty group. The fatty group had a lower polyunsaturated : saturated fatty acid ratio than the meaty and normal groups. In the subcutaneous adipose tissue, Krškopolje pigs had the highest proportion of C18:1 n-9 and differed from the normal group in C18:0 content, from the fatty group in C16:1 n-7 and from the meaty and fatty groups in C18:2 n-6. Furthermore, the Krškopolje pigs had the highest monounsaturated fatty acids (MUFAs), and lower PUFAs and n-6 PUFAs proportions than the fatty group. The meaty group had a higher n-6 : n-3 PUFA ratio than the Krškopolje pigs.

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Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660071 ◽

1985 ◽

Vol 54 (03) ◽

pp. 563-569 ◽

Cited By ~ 19

Author(s):

M K Salo ◽

E Vartiainen ◽

P Puska ◽

T Nikkari

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Platelet Aggregation ◽

Acid Composition ◽

Saturated Fatty Acids ◽

Free Living ◽

Ω3 Fatty Acids ◽

Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

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Effects of dietary roughage on organic pig performance, behaviour and antioxidants accretion in perirenal adipose tissue

Livestock Science ◽

10.1016/j.livsci.2020.104255 ◽

2020 ◽

Vol 241 ◽

pp. 104255

Author(s):

I. Argemí-Armengol ◽

D. Villalba ◽

Marc Tor ◽

Juan Ramón Bertolín ◽

M.A. Latorre ◽

...

Keyword(s):

Adipose Tissue ◽

Perirenal Adipose Tissue ◽

Pig Performance

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Effect of Sodium Nitrite on Growth of Shigella flexneri

Journal of Food Protection ◽

10.4315/0362-028x-54.6.424 ◽

1991 ◽

Vol 54 (6) ◽

pp. 424-428 ◽

Cited By ~ 23

Author(s):

LAURA L. ZAIKA ◽

ANNA H. KIM ◽

LOUISE FORD

Keyword(s):

Exponential Growth ◽

Shigella Flexneri ◽

Salt Content ◽

Brain Heart Infusion ◽

Growth Curves ◽

Inhibitory Effect ◽

Plate Count ◽

Generation Times ◽

High Salt Content ◽

Lag Times

A partial factorial design study of the effect of NaNO2 (0, 100, 200, 1000 ppm) in combination with NaCl (0.5, 2.5, 4.0%), pH (7.5, 6.5, 5.5), and temperature (37, 28, 19°C) on growth of Shigella flexneri is reported. Experiments were done aerobically in brain-heart infusion medium, using an inoculum of 1 × 103 CFU/ml. Growth curves were fitted from plate count data by the Gompertz equation; exponential growth rates, lag times, generation times, and maximum populations were derived for all variable combinations. In the absence of nitrite, the organism grew well under all test conditions at 37 and 28°C but did not grow at 19°C at pH 5.5 nor at pH 7.5 with 4% NaCl. Nitrite did not affect growth in media of pH 7.5 at 37 and 28°C. At pH 6.5 growth was inhibited by 1000 ppm NaNO2. The organism failed to grow at 19°C at all nitrite levels in the presence of 2.5 or 4.0% NaCl. The inhibitory effect of nitrite was much greater in media of pH 5.5 and increased with increasing salt levels. More inhibition was apparent at 28 than at 37°C. While lack of growth was used as a paradigm of the effect of nitrite on S. flexneri, nitrite also increased the lag and generation times and decreased the exponential growth rate. Results indicated that NaNO2 in combinations with low temperature, low pH, and high salt content can effectively inhibit the growth of S. flexneri.

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