scholarly journals ENVIRONMENTAL PERFORMANCE OF MICROBIAL FUEL CELL BASED LIVE SLUDGE FOR VOLTAGE PRODUCTION AND CONGO RED DYE REMOVAL

2022 ◽  
Vol 26 (1) ◽  
pp. 27-35
Author(s):  
Nabea M. Mahdi ◽  
◽  
Ahmed H. Ali ◽  

: In this work, Single chamber Microbial fuel cells (SCMFCs) are a versatile technology is depends on the interaction mechanisms of bacteria, to produce bioelectricity simultaneously and treat Congo red (CR) dye from aqueous solution at different pH (6.5-8). Electricity generation from the biodegradable organic substrate (sucrose) accompanied by decolorization of azo dye was investigated in the batch test results showed that more than 99% decolorization demonstrated at UV-Visible Spectrophotometer (500 nm) was achieved within 20 days and maximum output voltage (889 mv) had been obtained in an open circuit at a pH value of 7.5. Microbial community analysis showed that species in live sludge and the impact of bacteria grown on removal and voltage.

2019 ◽  
Vol 42 (5) ◽  
pp. 697-709 ◽  
Author(s):  
Caterina Armato ◽  
Daniyal Ahmed ◽  
Valeria Agostino ◽  
Deborah Traversi ◽  
Raffaella Degan ◽  
...  

2020 ◽  
Author(s):  
Ellen S. Cameron ◽  
Philip J. Schmidt ◽  
Benjamin J.-M. Tremblay ◽  
Monica B. Emelko ◽  
Kirsten M. Müller

AbstractThe application of amplicon sequencing in water research provides a rapid and sensitive technique for microbial community analysis in a variety of environments ranging from freshwater lakes to water and wastewater treatment plants. It has revolutionized our ability to study DNA collected from environmental samples by eliminating the challenges associated with lab cultivation and taxonomic identification. DNA sequencing data consist of discrete counts of sequence reads, the total number of which is the library size. Samples may have different library sizes and thus, a normalization technique is required to meaningfully compare them. The process of randomly subsampling sequences to a selected normalized library size from the sample library—rarefying—is one such normalization technique. However, rarefying has been criticized as a normalization technique because data can be omitted through the exclusion of either excess sequences or entire samples, depending on the rarefied library size selected. Although it has been suggested that rarefying should be avoided altogether, we propose that repeatedly rarefying enables (i) characterization of the variation introduced to diversity analyses by this random subsampling and (ii) selection of smaller library sizes where necessary to incorporate all samples in the analysis. Rarefying may be a statistically valid normalization technique, but researchers should evaluate their data to make appropriate decisions regarding library size selection and subsampling type. The impact of normalized library size selection and rarefying with or without replacement in diversity analyses were evaluated herein.Highlights▪ Amplicon sequencing technology for environmental water samples is reviewed▪ Sequencing data must be normalized to allow comparison in diversity analyses▪ Rarefying normalizes library sizes by subsampling from observed sequences▪ Criticisms of data loss through rarefying can be resolved by rarefying repeatedly▪ Rarefying repeatedly characterizes errors introduced by subsampling sequences


2013 ◽  
Vol 726-731 ◽  
pp. 2146-2150
Author(s):  
Ming Zhang ◽  
Zheng Jiang Zhu ◽  
Jun Mei He ◽  
Nai Dong Zhang

t was discovered that sunlight/Fe2+/S2O82- method is superior to UV/Fe2+/S2O82-method when we treated water samples of acidic Congo red .Congo red dye was applied in activated potassium persulfate by sunlight/ultraviolet and ferrous ion system investigation. It showed efficient sunlight/ultraviolet activated degradation of acidic Congo red, and the activation of sunlight was better than that of ultraviolet. The effect of pH value, Fe2+ and S2O82- concentration on the degradation of Congo red were investigated. The result indicated that when pH was 3.0, [Fe2+] was 0.42mmol/L, [S2O82-] was 74.0mmol/L, the decolouring ratio of acidic Congo red were 91.0% and 100% respectively after 5min and 60min of reaction times, the TOC removal ratio was 80.4% after 120min.


Author(s):  
Lauren V. Alteio ◽  
Joana Séneca ◽  
Alberto Canarini ◽  
Roey Angel ◽  
Ksenia Guseva ◽  
...  

Microbial community analysis via marker gene amplicon sequencing has become a routine method in the field of soil research. In this perspective, we discuss technical challenges and limitations of amplicon sequencing studies in soil and present statistical and experimental approaches that can help addressing the spatio-temporal complexity of soil and the high diversity of organisms therein. We illustrate the impact of compositionality on the interpretation of relative abundance data and discuss effects of sample replication on the statistical power in soil community analysis. Additionally, we argue for the need of increased study reproducibility and data availability, as well as complementary techniques for generating deeper ecological insights into microbial roles and our understanding thereof in soil ecosystems. At this stage, we call upon researchers and specialized soil journals to consider the current state of data analysis, interpretation and availability to improve the rigor of future studies.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Ragaa A. Hamouda ◽  
Muhammad A. Abuelmagd ◽  
Soad A. Abdelgalil

AbstractBecause of the increased amount of cobalt and Congo red dye effluents attributable to the industrial operations, the capacity of Enteromorpha intestinalis biomass as a sustainable source to achieve significant biosorption percent for both pollutants from dual solution was assessed. A fifty batch FCCCD experiments for biosorption of cobalt ions and Congo red dye were performed. The complete removal of Congo red dye was obtained at 36th run using an initial pH value of 10, 1.0 g/L of Enteromorpha intestinalis biomass, 100 and 200 mg/L of Congo red and cobalt for a 20-min incubation time. Meanwhile, a cobalt removal percent of 85.22 was obtained at 35th run using a neutral pH of 7.0, 3.0 g/L of algal biomass, 150 and 120 mg/L of Congo red, and cobalt for a 60-min incubation time. For further illustration and to interpret how the biosorption mechanism was performed, FTIR analysis was conducted to inspect the role of each active group in the biosorption process, it can be inferred that –OH, C–H, C=O, O–SO3- and C–O–C groups were mainly responsible for Co2+ adsorption of from aqueous dual solution. Also, scan electron microscope revealed the appearance of new shiny particles biosorbed on E. intestinalis surface after the biosorption process. EDS analysis proved the presence of Co2+ on the algal surface after the biosorption process.


2021 ◽  
Vol 8 (11) ◽  
Author(s):  
Mohamed Taha Amen ◽  
Ahmed S. Yasin ◽  
Mohamed I. Hegazy ◽  
Mohammad Abu Hena Mostafa Jamal ◽  
Seong-Tshool Hong ◽  
...  

The possibility of using rainwater as a sustainable anolyte in an air-cathode microbial fuel cell (MFC) is investigated in this study. The results indicate that the proposed MFC can work within a wide temperature range (from 0 to 30°C) and under aerobic or anaerobic conditions. However, the rainwater season has a distinct impact. Under anaerobic conditions, the summer rainwater achieves a promised open circuit potential (OCP) of 553 ± 2 mV without addition of nutrients at the ambient temperature, while addition of nutrients leads to an increase in the cell voltage to 763 ± 3 and 588 ± 2 mV at 30°C and ambient temperature, respectively. The maximum OCP for the winter rainwater (492 ± 1.5 mV) is obtained when the reactor is exposed to the air (aerobic conditions) at ambient temperature. Furthermore, the winter rainwater MFC generates a maximum power output of 7 ± 0.1 mWm −2 at a corresponding current density value of 44 ± 0.7 mAm −2 at 30°C. While, at the ambient temperature, the maximum output power is obtained with the summer rainwater (7.2 ± 0.1 mWm −2 at 26 ± 0.5 mAm −2 ). Moreover, investigation of the bacterial diversity indicates that Lactobacillus spp. is the dominant electroactive genus in the summer rainwater, while in the winter rainwater, Staphylococcus spp. is the main electroactive bacteria. The cyclic voltammetry analysis confirms that the electrons are delivered directly from the bacterial biofilm to the anode surface and without mediators. Overall, this study opens a new avenue for using a novel sustainable type of MFC derived from rainwater.


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