The effect of increased UV-A radiation during the ontogeny of barley plants of the Vladimir variety in the vegetation experiment was studied. Changes in the content of malonic dialdehyde, flavonoids and grain yield were revealed. UV-A radiation as compared to UV-B radiation, has lower quantum energy and can have both positive and negative effects on plant regulatory and photosynthetic processes. One of the most damaging effects of increased levels of UV-A radiation is oxidative stress, which causes lipid peroxidation of biological membranes. The existence of a plant cell in such conditions is possible only thanks to a system of antioxidant defense mechanisms. The accumulation of phenolic compounds under the action of UV radiation is a universal mechanism of protection against photodamage, which was formed in the early stages of the evolution of photoautotrophic organisms. Flavonoids are localized in the epidermis of plant tissues and act as an internal filter. The content of flavonoids is determined by the genotype and due to ontogenetic patterns. Plants were grown in a greenhouse, in vessels containing 4.5 kg of air-dry soil. The repetition is threefold (3 vessels in each variant). Sowing density - 13 plants in each vessel. As a source of UV-A radiation used lamps Black Light BLUE company Philips. Plants were irradiated for 5 hours a day from 10 to 15 hours at 13, 25, 34, 43 and 52 stages of organogenesis. The magnitude of the daily biologically effective dose of UV-A radiation was 60.7 kJ / m2. The solar part of the UV spectrum in the vegetation experiment was absent in the greenhouse. The nature of changes in the content of flavonoids under the action of UV-A irradiation during the growing season of plants with the dynamics of the oxidative process has been established. The first maximum was observed during the vegetative growth period, the second - at the earing stage. The data obtained indicate that flavonoids have ontogenetic conditionality and perform photoprotective functions. The increase in their content under the action of UV-A radiation is accompanied by an increase in resistance to photodamage, which is confirmed by the formation of grain yield.
Biosynthesis of basement membrane collagen by rabbit corneal endothelium in vitro.
