The state of the enzymatic antioxidant system of the lens and anterior chamber humor in cataract modeling against the background of allergic uveitis after using mexidol

Along with specific adaptive reactions, universal defense reactions, in particular activation of antioxidant system, are of great importance for plant survival under cold conditions. We have studied a relationship among the content of low-molecular-weight protective compounds with antioxidant properties (proline, soluble carbohydrates, flavonoids), the activity of antioxidant enzymes (superoxide dismutase, catalase, and guaiacol peroxidase) in seedlings of winter wheat, rye and triticale, and frost resistance of etiolated seedlings and adult plants at tillering stage. It was found that there was a fairly close correlation between the frost resistance of seedlings and adult cereal plants (r = 0,78). It was shown that a pronounced relationship between individual indicators of antioxidant system functioning in unhardened seedlings and their frost resistance was not found. After 6-day hardening of seedlings at 2-4°C, there was a high correlation between the total indicator of the enzymatic antioxidant system (the sum of normalized indicators of superoxide dismutase, peroxidase, and catalase activity) and their frost resistance (r = 0,86), but the correlation coefficient of this index with frost resistance of plants in tillering phase was significantly lower (r = 0,47). At the same time, a high correlation was found between the content of low-molecular-weight protectors in hardened seedlings and frost resistance of tillering adult plants (r = 0.89). The closest correlation was observed between the integral normalized indicator, comprising the sum of normalized values of antioxidant enzymes activity and the content of low-molecular-weight protectors in hardened seedlings, and frost resistance of seedlings (r = 0,94) and plants in tillering phase (r = 0,89). A presence of specific features in the functioning of antioxidant system during cold adaptation of cereal seedlings was established. Rye is characterized by a high content of low-molecular-weight protective compounds; at the same time, increased activity of antioxidant enzymes - superoxide dismutase and catalase - was noted in wheat seedlings. In triticale, depending on the genotype, the values of both enzymatic antioxidant activity and the content of low-molecular-weight protectors varied.

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Indicators of the antioxidant system in the long-term period after acute mercury nitrate poisoning in the experiment

Токсикологический вестник ◽

10.36946/0869-7922-2020-2-35-40 ◽

2020 ◽

pp. 36-41

Author(s):

E. G. Batotsyrenova ◽

O. A. Vakunenkova ◽

E. A. Zolotoverkhaya ◽

V. A. Kashuro ◽

T. A. Kostrova ◽

...

Keyword(s):

Antioxidant System ◽

Antioxidant Defense ◽

Reduced Glutathione ◽

Antioxidant Defense System ◽

Mercury Content ◽

The State ◽

Cytotoxic Effects ◽

Entire Study ◽

Mercury Nitrate

The article presents experimental data on the state of the antioxidant system in red blood cells of white outbred rats 1 and 3 months after acute mercury nitrate poisoning with a semilethal dose. It has been established that this form of intoxication is accompanied by pronounced changes in the state of the antioxidant defense system in erythrocytes of poisoned animals (a decrease in the concentration of reduced glutathione, a decrease in the activity of superoxide dismutase and glutathione peroxidase, and an increase in the concentration of lipid peroxidation products).It has been shown that the mercury content in the blood of experimental animals remains elevated during the entire study period.The results obtained indicate the importance of impaired functioning of the antioxidant system in the implementation of long-term consequences of acute mercury poisoning. The reasons for the occurrence of these biochemical shifts and their role in the development of the long-term cytotoxic effects of mercury nitrate are discussed.

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The condition of the enzymatic antioxidant system in the retina in experimental diabetes and application of vitamin B6

Oftalmologicheskii Zhurnal ◽

10.31288/oftalmolzh201137378 ◽

2011 ◽

Vol 31 (3) ◽

pp. 73-78

Author(s):

K. Pavlyuchenko ◽

◽

S. Mogilevskyy ◽

A. Chuyko ◽

◽

...

Keyword(s):

Antioxidant System ◽

Vitamin B6 ◽

Experimental Diabetes ◽

Enzymatic Antioxidant

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THE STATE OF THE ANTIOXIDANT SYSTEM OF THE INTERNAL ORGANS IN RATS DURING BURN DISEASE

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2014-3-51-55 ◽

2014 ◽

Vol 13 (3) ◽

pp. 51-55

Author(s):

L. G. Netyukhailo ◽

T. A. Sukhomlin ◽

Ya. A. Basarab ◽

V. V. Bondarenko ◽

S. V. Kharchenko

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Free Radical ◽

Salivary Glands ◽

Antioxidant System ◽

The State ◽

Antioxidant Systems ◽

Free Radical Processes ◽

Burn Disease ◽

Radical Processes

The objective of research was to study the state of prooxidant and antioxidant systems in the tissues of the lungs, kidneys, pancreas and salivary glands at burn disease. The intensity of the free radical processes was evaluated on the basis of the content of malondialdehyde (MDA) and antioxidant system – based on the indexes of its enzymatic chain: superoxide dismutase and catalase in homogenates of the studied organs. It has been found that changes in experimental burn disease depend on the studied organs and the stage of burn disease. The activation of free radical processes observed in all investigated organs (lungs, kidneys, pancreas and salivary glands). Reactive oxygen species induce lipid peroxidation, which is a universal marker of tissue damage. MDA appears in the body during degradation of polyunsaturated fatty acids and it’s a marker of lipid peroxidation and oxidative stress. It was found the increasing of MDA in all organs, especially in the lungs and kidneys at stage of burn shock. Under these conditions it was observed the decrease of superoxide dismutase and catalase in all investigated organs. At burn disease there is development of disbalance between the action of prooxidant and antioxidant systems due to the activation of free radical processes.

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Antioxidant enzyme activities in rabbits under oxidative stress induced by high fat diet

Journal of Veterinary Research ◽

10.2478/jvetres-2018-0019 ◽

2018 ◽

Vol 62 (2) ◽

pp. 199-205 ◽

Cited By ~ 1

Author(s):

Marta Skowron ◽

Jolanta Zalejska-Fiolka ◽

Urszula Błaszczyk ◽

Ewa Chwalińska ◽

Aleksander Owczarek ◽

...

Keyword(s):

Antioxidant Enzyme ◽

Antioxidant System ◽

Oxidative Status ◽

Antioxidant Enzyme Activities ◽

High Fat ◽

Enzymatic Antioxidant ◽

High Fat Diets ◽

Fat Diets ◽

Increased Activity ◽

Total Superoxide Dismutase

AbstractIntroductionThe aim of this study was to investigate whether the type and form of oil (raw/non-oxidised (N) or post-frying/oxidised (O)) consumed in high-fat diets affect the oxidative status of an organism, as observed by malondialdehyde (MDA) concentration as an oxidative factor and antioxidant enzyme activity.Material and MethodsFats in the diet came from rapeseed oil (R) and olive oil (O).ResultsThe applied diet caused a decrease in MDA concentration (μmol/L) in serum in group RN from 2.94 ± 0.87 to 1.76 ± 0.13, in group ON from 2.45 ± 0.62 to 1.50 ± 0.10, and in group OO from 2.70 ± 1.16 to 1.84 ± 0.36. Meanwhile, MDA concentration (mmol/L) increased in blood haemolysate in group RO from 0.15 ± 0.07 to 0.22 ± 0.03 and in group OO from 0.17 ± 0.02 to 0.22 ± 0.02. The observed changes caused a response of the enzymatic antioxidant system in both models, especially followed by an increase in activities of total superoxide dismutase and its mitochondrial isoenzyme in all experimental groups, while its cytosolic isoenzyme activity increased only in ON and OO groups. Increased activity of glutathione peroxidase (GPX) in groups RN and RO and of catalase (CAT) in groups ON and OO was observed. Significant differences in responses to the different types and forms of oils were probably caused by the different oxidative stability of the studied oils.ConclusionThis diet disturbed the body’s oxidative status; however, during the six-month study the enzymatic antioxidant system remained effective.

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