Pre-Sowing Treatments Accelerate Germination Percent for Restoration of Fourteen Threatened Tree Species in Bangladesh

Ecologically valuable native tree species are becoming threatened due to deforestation, forest fragmentation and preference of fast-growing exotics than the native ones in plantation. One of the main reasons for the preference of exotic species than the native ones is its higher rate of germination and rapid growth. The effect of different pre-sowing treatments was studied on fourteen threatened tree species of Bangladesh to find out the appropriate treatments for speed up germination rate and initial seedling growth. These species are ecologically valuable multipurpose indigenous trees of Bangladesh. Methanol extract of Castanopsis indica leaves could decrease the tumor Ehrlich Ascites Carcinoma volume and weight. Lophopetalum wightianum is a globally threatened tree species. Hard coated seeds of Canarium resiniferum, Castanopsis indica, Protium serratum, Quercus acuminata and Vitex peduncularis were treated with sand paper, nicking, normal water, hot water, H2SO4 and HCl. Soft coated seeds of Brownlowia elata, Dichopsis polyantha, Firmiana colorata, Lophopetalum wightianum, Pterospermum acerifolium, Pterospermum semisagittatum, Pterygota alata, Schleichera oleosa and Sterculia villosa were sown in polybags, propagator house and nursery bed in normal, flat and 45o angle positions. Among all hard-coated seeds, Castanopsis indica showed significantly higher germination percentage (67%) after seeds treated with sandpaper in comparison to control (25%). Soft-coated seeds of Lophopetalum wightianum showed significantly higher germination percentage (90%) among all studied species when sown in propagator house, whereas in natural condition it provides only 26% germination rate. This paper will help to restore ecologically valuable threatened species.

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Fourteen Threatened Native Trees of Bangladesh: Which Pretreatment Generates Maximum Germination Percent?

Journal of Non Timber Forest Products ◽

10.54207/bsmps2000-2015-a8wjs2 ◽

2015 ◽

Vol 22 (2) ◽

pp. 83-86

Author(s):

G.N. Hasnat ◽

M. Hossain ◽

M. Bhuiyan ◽

M. Alam

Keyword(s):

Tree Species ◽

Cold Water ◽

Germination Rate ◽

Hot Water ◽

Species Response ◽

Native Trees ◽

Native Tree ◽

Speed Up ◽

Native Tree Species ◽

Castanopsis Indica

Effect of different pre-sowing treatments on germination of fourteen threatened native tree species of Bangladesh was studied to find out the appropriate pre-sowing treatments to speed up germination rate of the required and desired species. Hard coated fruits and seeds of Canarium resiniferum, Castanopsis indica, Protium serratum, Quercus acuminata and Vitex peduncularis were treated with sand paper, nicking, cold water, hot water, H2SO4 and HCl. Seeds of Brownlowia elata, Dichopsis polyantha, Firmiana colorata, Lophopetalum fimbriatum, Pterospermum acerifolium, Pterospermum semisagittatum, Pterygota alata and Sterculia villosa were sown in polybags, propagator house and nursery bed in normal, flat and in 450 angle position. Hard fruits of Castanopsis indica sown in polybags without any treatment showed 25% germination but increased to 67% when fruits were treated with sand paper rubbing at the distal end. Seeds of Lophopetalum fimbriatum germinated only 26% when sown normally in polybags but when sown in propagator house it increased to 90%. Different species response differently with pre-sowing treatments but in general pre-sowing treatments increases the germination percent in all the species.

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A Novel Method to Overcome Coat-Imposed Seed Dormancy in Lupinus albus L. and Trifolium pratense L.

Journal of Botany ◽

10.1155/2014/647469 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Iskender Tiryaki ◽

Mustafa Topu

Keyword(s):

Seed Dormancy ◽

Trifolium Pratense ◽

Germination Rate ◽

Lupinus Albus ◽

Red Clover ◽

Treatment Method ◽

Germination Percentage ◽

Hot Water ◽

White Lupin ◽

Novel Method

We have developed a novel method to overcome coat-imposed seed dormancy in legume plants. Seeds of Lupinus albus L. and Trifolium pratense L. were stored in a freezer at −80°C for a period of time and then immediately treated with or without hot water at 90°C for 5 seconds. Germination tests were carried out in darkness at 20±1.0°C with four replications in a completely randomized design. Final germination percentage (FGP), germination rate, and synchrony of seeds were evaluated. The results showed that new approach of freeze-thaw scarification provided high percentage of germinations in white lupin (84.16%) and red clover (74.50%) seeds while control seeds had FGPs of 3.3% and 26.0%, respectively. The immediate thawing of frozen seeds in hot water for 5 seconds was found not only an effective and reliable but also the quickest seed treatment method to prevail against coat-imposed seed dormancy in legume species and may become operationally applicable to other plant species.

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In-vitro Seed Germination Biology of Milicia Excelsa Population Collected from Benchi-Maji Zone, South Western Ethiopia

10.21203/rs.3.rs-36599/v1 ◽

2020 ◽

Author(s):

MOHAMMED ADEFA SEID ◽

Yigardu Mulatu ◽

Agena Anjulo ◽

Semaigzer Ayalew ◽

Marshet Nigatu ◽

...

Keyword(s):

Seed Germination ◽

Tap Water ◽

Germination Rate ◽

Germination Percentage ◽

Hot Water ◽

Laboratory Conditions ◽

Southwestern Ethiopia ◽

Micro Propagation ◽

Milicia Excelsa

Abstract Background: In Ethiopia, species used for timber production are limited to few species. So, it is becoming necessary to find innovative ways of maintaining and improving the genetic quality of species such as Milicia excelsa by domesticating them into a wider scale of ecological and social environment. Hence, the main objective of this study was to investigate the seed germination biology and behavior of Milicia excelsa under in-vitro laboratory conditions so as to promote the domestication and establishment of seed production areas (SPA) in-situ. Methods: Seeds collected from Benchi district of Benchi-Maji zone of southwestern Ethiopia were subjected to germination biology in-vitro to determine the germination responses of M. excelsa seeds under laboratory conditions. So, a total of 1200 seeds were randomly selected, treated and prepared using four treatments procedures with six replications for paper method and 3 replications for sand method, and tested using paper methods in CRD design. So, 600 seeds were tested using paper and sand methods each where 25 seeds were tested for each replication in paper and 50 seeds for each replication in sand method. The germination process was lasted between 19 March 2020 and 15 May 2020. Result: The mean germination percentage of seeds of M. excelsa was noticed to be lower than 20%. This is below the expected (45%) germination rate of mature and healthy seed lot. Overall, seeds that were not treated using any pre-sowing treatments has shown relatively better germination percentage (19%) followed by seeds rubbed by hand (15%). Analysis of one-way t-test shows that the effects untreated (control –T1) and rubbing by hand treatments (T4) is significant at p < 0.05 compared to the effects of the other pre-sowing treatments (T2- seeds only washed with normal tap water, and T3- seeds washed and soaked in hot water for 15 minutes) on the germination capability of seeds of M. excelsa . However, no significance difference was observed between control (T1) and hand scarification (T2). Conclusions: The average germination of M. excelsa seeds combined for all treatments is lower than 20% which is lower germination when comparing with other similar research reported in other countries. Therefore, it required to include and test other pre-sowing treatments including tissue culture micro-propagation of seeds to maximize the germination of M. excelsa seeds helping to get enough seedlings required for the domestication project.

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EFFECT OF SEED PRIMING METHODS ON THE GERMINATION OF DETARIUM MICROCARPUM

International Journal of Advanced Academic Research ◽

10.46654/ij.24889849.e7518 ◽

2021 ◽

pp. 1-10

Author(s):

A Abdulrahman ◽

M Atiku ◽

A.S Ambursa ◽

I Umar ◽

M Muhammed ◽

...

Keyword(s):

Tap Water ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

Hot Water ◽

State University ◽

Detarium Microcarpum ◽

Randomized Design ◽

Completely Randomized Design ◽

Pre Treatment

This study was carried out at Kebbi State University of Science and Technology, Aliero, Kebbi State, to determine the effect of seed priming methods on the germination of Detarium microcarpum. The study was carried out in the Forestry Laboratory of the Faculty of Agriculture. The effectiveness of nine (9) different seed priming methods was assessed, control inclusive. Treatment 1 (TRT1) seeds were soaked in tap water for 48 hours, Treatment 2 (TRT2) seeds were mechanically scarified opposite to microphyle, treatment 3, 4, and 5 (TRT3, TRT4, TRT5) seeds were immersed in H2SO4 for 5, 10 and 15 minutes respectively, treatment 6, 7 and 8 (TRT6, TRT7 and TRT8) seeds were soaked in hot water (100oC) for 5, 10 and 15 minutes respectively and untreated seeds (CNTRL). Completely Randomized Design with three (3) replications was used. The data obtained were analyzed using one-way Analysis of Variance (ANOVA).The result revealed that seeds of D. microcarpum subjected to TRT2, TRT4, TRT5 and TRT8 recorded with highest Final Germination Percentage (FGP 100%) and was found to differ significantly (P<0.05) with other treatment and CNTRL recorded the lowest FGP (60%).Thus, TRT2 was significantly (P<0.05) enhanced the seed and germination rate of D. microcarpum with the highest FGP (100%), lowest MGT (6.67), highest GRI (15.05), highest GE (12.01), lower FDG (5.67) with maximum GI value (900). Based on the result, pre-treatment of T. indica seeds with H2SO4 is recommended for effective germination.

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Effect of Pre-Sowing Treatments on Seed Germination and Seedling Growth of Silver Butterfly Tree (Bauhinia rufescens)

Current Agriculture Research Journal ◽

10.12944/carj.6.3.13 ◽

2018 ◽

Vol 6 (3) ◽

pp. 344-354

Author(s):

J. A. Opoku ◽

J. N. Amissah ◽

M. E. Essilfie ◽

J. C. Norman

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Growth Regulator ◽

Tap Water ◽

Germination Rate ◽

Germination Percentage ◽

Hot Water ◽

Root Weight ◽

Number Of Leaves ◽

Shoot Weight

Three experiments were conducted in a plastic greenhouse at the Sinna Garden of the Crop Science Department of the University of Ghana, Legon, Accra to investigate into pre-sowing treatment (water treatment, growth regulator, and sulphuric acid scarification and water soaking) effects on seed germination and seedling growth of Bauhinia rufescens from October to December, 2011 and January to April, 2012, respectively. The experimental design used was complete randomized design. There were 4 treatments in experiment 1, 7 in experiment 2 and 6 in experiment 3. Each experiment was replicated five (5) times. The results showed that Bauhinia rufescens seeds soaked in hot water at 65 0 C for 60 minutes gave the highest mean germination rate, tallest plant height and highest number of leaves per plant at 49 days after soaking. It also had the widest stem diameter, highest fresh shoot and root weight, highest fresh root and dry root weight and number of roots per seedling. Seeds soaked in 500 ppm Gibberelic acid(GA3) produced the highest number of leaves per plant and tallest plants at 49 DAS. Seeds soaked in 750 ppm GA3 had the highest number of leaves per plant at 49 DAS and highest fresh shoot weight. Seeds soaked in 1000 ppm Promalin differed significantly from 500 ppm GA3 and the control in germination percentage. Seeds soaked in 1000 ppm Promalin differed significantly from 500 ppm GA3 and the control in germination percentage. Acid scarified seeds for 60 minutes plus soaking in tap water for 24 hours differed significantly from the seeds treated withother growth regulator rates plus 24 hours soaking in germination percentage. Acid scarified seeds for 45 minutes plus soaking in tap water for 24 hours had the tallest plant and highest number of leaves per plant at 49 DAS, widest stem diameter, highest fresh shoot and root weight and dry shoot weight. Soaking seeds in hot water at 65 0 C for 60 minutes and/or in acid (H2SO4) for 45 minutes plus soaking in tap water for 24 hours is recommended as pre-sowing treatment for Bauhinia rufescens for maximum mean germination rate and vigorous vegetative growth of seedlings.

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EFFECT OF DIFFERENT PRE-SOWING TREATMENT ON THE EMERGENCE OF DIALIUM GUINEENSE (WILD)

African Journal of Sustainable Agricultural Development ◽

10.46654/2714.141226 ◽

2020 ◽

pp. 51-59

Author(s):

A Abdulrahman ◽

M Atiku ◽

AS Ambursa ◽

I Umar ◽

A Shuaibu ◽

...

Keyword(s):

Large Scale ◽

Cold Water ◽

Low Cost ◽

Germination Rate ◽

Germination Percentage ◽

Hot Water ◽

Scale Production ◽

Randomized Design ◽

Pre Treatment ◽

Result Analysis

The experiments were conducted in the Forestry laboratory of Kebbi State University of Science and Technology, Aliero, Kebbi State. The studies were conducted from February 2020− March 2020 to assess the most effective method of breaking seed dormancy in Dialium guineense seeds. The seeds were subjected to four (4) pre-treatment methods (sulphuric acid, hot water, cold water and mechanical scarification) with untreated seeds as control, the experiment was laid out in completely randomized design (CRD) and replicated three (3) times. Final germination percentage (FGP), germination mean time (GMT), germination index (GRI), Coefficient Velocity of Germination (CVG) and Germination rate index (GRI) were calculated and the data was subjected to the analysis of the variance (ANOVA). Seed treated with cold water for 48 hours (Trt 1) started germination at two (2) days after sowing (DAS) and ended germination at 5 DAS, control and three of the treatments (Trt 2, Trt 5& Trt 7) started germination at 3 DAS while Trt 4 and Trt 8 started at 4 and 5 DAS respectively. FGP 100% was obtained from Control and Trt 5 and they finished Germination at 9 and 21 DAS respectively. Highest GI, GRI and CVG and lowest MGT were recorded from Trt 1. The result analysis of findings at 5% level of statistics showed that there is nosignificant difference between the treatments. Similarly, no germination on seeds in Trt 3 and Trt 6. Seeds of Dialium guineense are not limited by dormancy therefore can be sown without any pre-treatment, but for large scale production of Dialium seedlings it can be treated in cold water at room temperature for 48 hours due to availability and low cost with less risks.

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Pengaruh Perlakuan Pencelupan Dan Perendaman Terhadap Perkecambahan Benih Sengon (Paraserianthes falcataria L.)

Agrologia ◽

10.30598/a.v2i1.273 ◽

2018 ◽

Vol 2 (1) ◽

Author(s):

Marthen Marthen ◽

Elizabet Kaya ◽

Herman Rehatta

Keyword(s):

Seed Dormancy ◽

Seed Coat ◽

Cold Water ◽

Germination Rate ◽

Germination Percentage ◽

Hot Water ◽

Hard Seed ◽

Randomized Design ◽

Vigor Index ◽

Two Factors

In developing productive forests, seeds play a crucial role. The seeds of forests tree have physical condition of hard seed coat, especially Leguminoceae family. Silvicultural techniques that can overcome seed coat dormancy are dyeing and submersion of seeds. The knowledge and experience in breaking seed dormancy of Sengon (Paracerianthes falcataria L.) is rare, therefore it is necessary to study how to overcome seed dormancy. The objective of this study was to determine the effect of dyeing and submersion treatments to overcome seed dormancy of Sengon seed to improve its viability. This research used Completely Randomized Design (CRD) with two factors: namely Seed (Factor A), dipped in hot water at 60 º C for (A0 = Control, A1 = 2 minutes, A2 = 4 minutes, A3 = 6 minutes), Factor B, seeds soaked in cold water: (B0 = control, B1 = 6 hours of soaking, B2 = 12 hours, B3 = 18 hours, B4 = 24 hours). The parameters observed were germination percentage, germination rate and vigor index. The results showed that among all parameters observed the interaction of factors A1B2, A2B2 and A3B2 were the best treatment for germination percentage which was equal to 100 percent, the best germination rate of 3.897 days and the best vigor index of 27.02

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THE EFFECT OF THREE PRETREATMENTS ON BREAKING SEED DORMANCY OF BAOBAB (ADANSONIA DIGITATA L.)

Innovare Journal of Agricultural Sciences ◽

10.22159/ijss.2021.v9i3.40759 ◽

2021 ◽

pp. 1-3

Author(s):

BASHIR KA ◽

MUSA DD ◽

BISHIR R

Keyword(s):

Biological Sciences ◽

Seed Dormancy ◽

Germination Rate ◽

Germination Percentage ◽

Hot Water ◽

Limiting Factor ◽

Adansonia Digitata ◽

Endangered Tree ◽

Randomized Design ◽

Pre Treatment

Objective: Baobab (Adansonia digitata) is a native tree that is found in African savannas, it is a member of family ‘Malvaceae’. The tree is found in Nigeria, South Africa, Botswana, Namibia, Mozambique and in different regions of western Madagascar. Seed dormancy is the most limiting factor for some plants propagation. Dormancy allows seeds to separate from their mother plant and survive dispersal, over distance, and time before growth recommences. This study was aimed to determine the effect of three pre-treatments on breaking the seed dormancy of Adansonia digitata L., a multi-purpose and indigenously endangered tree. Methods: The study was carried out in the greenhouse of the Department of Biological Sciences, Federal University Dutsin-Ma, Nigeria. The three pre-treatments are: Soaking in hot water (100°C) for 5, 10, 15, and 20 min; and soaking in sulfuric acid (H2SO4) and hydrochloric acid (HCL) concentrations (100%, 75%, 50%, and 25%) for 5, 10, 15, and 20 min. Untreated seeds served as control. These were laid out in a complete randomized design with three replications. Data were collected on germination percentage, germination rate, day of emergence, and the average seedlings height. Results: The result showed that earliest mean days of emergence (7 days) were observed in seeds subjected to hot water treatment at 15 min. It also showed the highest rate of germination and percentage 6 (100%). On the other hand, the effect of acids (H2SO4 and HCL) pre-treatments on the seeds gave a fair result (16.66–33.33%), untreated seeds did not germinate. Conclusion: All the pre-treatments used in the study were found to be effective. However, soaking of seeds in hot water (100°C) pre-treatment for 15 min is recommended for breaking the seed dormancy of A. digitata.

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Dormancy, storability, and germination of seeds in Magnolia punduana (Magnoliaceae)

Botany ◽

10.1139/cjb-2016-0056 ◽

2016 ◽

Vol 94 (10) ◽

pp. 967-973 ◽

Cited By ~ 5

Author(s):

Viheno Iralu ◽

Krishna Upadhaya

Keyword(s):

Tree Species ◽

Germination Percentage ◽

Cold Stratification ◽

Seed Length ◽

Morphophysiological Dormancy ◽

Breaking Dormancy ◽

Physical Treatments ◽

Germinating Seeds ◽

Embryo Length ◽

Threatened Tree Species

Magnolia punduana Hk. f. & Th. (syn. Michelia punduana) is a threatened tree species restricted to northeastern India. The seeds remain dormant for 6 months after dispersal. Seed development and breaking of dormancy was investigated in the present study. Physical treatments such as water (cold, hot, and boiling) and acid and manual scarification proved ineffective in breaking dormancy. Matured seeds had linear and underdeveloped embryos at the time of dispersal with an embryo to seed length (E:S) ratio of 0.13. Germinating seeds showed a significant increase in the embryo length with an E:S ratio of 0.36. Cold stratification (CS) at 5 °C effectively broke dormancy. The embryo grew significantly after CS, indicating the presence of “non-deep simple” morphophysiological dormancy (MPD). Application of GA3 had a significant effect on germination and the lowest germination time with the shortest T50 was observed in seeds treated with 3000 mg·L−1 GA3. KNO3 did not have an influence on breaking dormancy except in combination with GA3, which increased the germination percentage significantly. CS for 90 days and GA3 (3000 mg·L−1) at 25 °C resulted in optimum germination. In the test for viability, seeds stored at 5 °C showed highest viability of 66.66% after 180 days.

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Tratamentos pré-germinativos em sementes de Sapindus saponaria L

Revista Agraria Academica ◽

10.32406/v3n52020/147-154/agrariacad ◽

2020 ◽

Vol 3 (5) ◽

pp. 147-154

Author(s):

Sérgio Roberto Garcia dos Santos ◽

◽

Renata Salaro Stecca ◽

Luciana Cantanhede de Souza ◽

Sebastiana Dutra Souza Revoredo Silva ◽

...

Keyword(s):

Hydrochloric Acid ◽

Tree Species ◽

Germination Percentage ◽

Hot Water ◽

Germination Speed ◽

Sapindus Saponaria

Sapindus saponaria, a brazilian tree species, known as soldier soap, is used in civil construction, landscaping, urban afforestation and reforestation. The objective of this study with this species was to identify the most efficient pre-germinative treatments for breaking the dormancy of its seeds. For this purpose, eight treatments plus the control were tested. The parameters used were the germination percentage and the germination speed index (IVG). As a result, based on the IVG, it was found that the best treatments were: hydrochloric acid, sandpaper and fire, not differing statistically from each other, the first two being superior to the control. For germination and IVG, acetone and hot water showed lower results than the control.

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