Maltotriose-conjugated Chicken Myofibrillar Proteins Derived from Random-centroid Optimization Exhibit Potent Solubility in Low Ionic Strength Medium

2020 ◽  
Vol 26 (6) ◽  
pp. 759-769
Author(s):  
Kimio Nishimura ◽  
Momoka Suzuki ◽  
Hiroki Saeki
Keyword(s):  
Ionic Strength ◽  
Myofibrillar Proteins ◽  
Low Ionic Strength ◽  
Strength Medium

Improved Solubility of Carp Myofibrillar Proteins in Low Ionic Strength Medium by Glycosylation

1997 ◽  
Vol 45 (9) ◽  
pp. 3419-3422 ◽  
Author(s):  
Hiroki Saeki ◽  
Keisuke Inoue
Keyword(s):  
Ionic Strength ◽  
Myofibrillar Proteins ◽  
Low Ionic Strength ◽  
Strength Medium

Differential Coating of Human Red Blood Cells with C4 or C3 in a Low Ionic Strength Medium

Vox Sanguinis ◽  
1981 ◽  
Vol 41 (4) ◽  
pp. 249-254 ◽  
Author(s):  
H. E. Heier ◽  
L. Kornstad ◽  
R. Nordhagen
Keyword(s):  
Ionic Strength ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Low Ionic Strength ◽  
Strength Medium

Increased Sensitivity of Tests for the Detection of Blood Group Antigens in Stains Using a Low Ionic Strength Medium

1978 ◽  
Vol 18 (1) ◽  
pp. 16-23 ◽  
Author(s):  
M. J. McDowall ◽  
P. J. Lincoln ◽  
B. E. Dodd
Keyword(s):  
Ionic Strength ◽  
Blood Group ◽  
Red Cells ◽  
Blood Group Antigens ◽  
Additional Advantage ◽  
Suspension Medium ◽  
Increased Sensitivity ◽  
Low Ionic Strength ◽  
Strength Medium

The incorporation of a low ionic strength solution (LISS) in the micro-elution technique used for the detection of blood group antigens in stains markedly improves the test's sensitivity. This is because LISS increases the amount of antibody taken up by the antigen in the stain which results in a greater yield of antibody recovered from the slain by elution. LISS also enhances the activity of the eluted antibody if it is introduced as a suspension medium for the red cells used to detect the antibody. The introduction of suitably diluted AB serum as diluent when testing the eluates is an additional advantage. The improvement in the sensitivity of the micro-elution technique is great enough in some instances to allow the detection of an antigen in a stain which is undetectable in the absence of LISS. Moreover some doubtful positive reactions are enhanced sufficiently for the presence of an antigen to be definitely established.

scholarly journals A Mutant of Sindbis Virus Which Is Released Efficiently from Cells Maintained in Low Ionic Strength Medium

Virology ◽  
1995 ◽  
Vol 210 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Mei-Ling Li ◽  
Victor Stollar
Keyword(s):  
Ionic Strength ◽  
Sindbis Virus ◽  
Low Ionic Strength ◽  
Strength Medium

Insights into the structural characteristic of rabbit glycated myofibrillar protein with high solubility in low ionic strength medium

LWT ◽  
2021 ◽  
Vol 137 ◽  
pp. 110387
Author(s):  
Shaobo Li ◽  
Zhifei He ◽  
Cheng Qu ◽  
Sijie Yu ◽  
Minhan Li ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Structural Characteristic ◽  
Myofibrillar Protein ◽  
High Solubility ◽  
Low Ionic Strength ◽  
Strength Medium

Alteration of Cellular Features after Exposure to Low Ionic Strength Medium

2020 ◽  
pp. 163-179
Author(s):  
Ingolf Bernhardt
Keyword(s):  
Ionic Strength ◽  
Low Ionic Strength ◽  
Strength Medium

l -histidine improves water retention of heat-induced gel of chicken breast myofibrillar proteins in low ionic strength solution

2016 ◽  
Vol 51 (5) ◽  
pp. 1195-1203 ◽  
Author(s):  
Xing Chen ◽  
Yong Li ◽  
Ruiyun Zhou ◽  
Zhaiming Liu ◽  
Fengzhi Lu ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Water Retention ◽  
Chicken Breast ◽  
Myofibrillar Proteins ◽  
Low Ionic Strength

scholarly journals Contributions of MexAB-OprM and an EmrE Homolog to Intrinsic Resistance of Pseudomonas aeruginosa to Aminoglycosides and Dyes

2003 ◽  
Vol 47 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Xian-Zhi Li ◽  
Keith Poole ◽  
Hiroshi Nikaido
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ionic Strength ◽  
Ethidium Bromide ◽  
Luria Broth ◽  
Drug Susceptibility ◽  
Intrinsic Resistance ◽  
Aminoglycoside Resistance ◽  
Active Efflux ◽  
Low Ionic Strength ◽  
Strength Medium

ABSTRACT Of the six putative small multidrug resistance (SMR) family proteins of Pseudomonas aeruginosa, a protein encoded by the PA4990 gene (emrE Pae) shows the highest identity to the well-characterized EmrE efflux transporter of Escherichia coli. Reverse transcription-PCR confirmed the expression of emrE Pae in the wild-type strain of P. aeruginosa. Using isogenic emrE Pae, mexAB-oprM, and/or mexB deletion mutants, the contributions of the EmrE protein and the MexAB-OprM efflux system to drug resistance in P. aeruginosa were assessed by a drug susceptibility test carried out in a low-ionic-strength medium, Difco nutrient broth. We found that EmrEPae contributed to intrinsic resistance not only to ethidium bromide and acriflavine but also to aminoglycosides. In this low-ionic-strength medium, MexAB-OprM was also shown to contribute to aminoglycoside resistance, presumably via active efflux. Aminoglycoside resistance caused by these two pumps could not be demonstrated in high-ionic-strength media, such as Luria broth or Mueller-Hinton broth. The EmrE-dependent efflux of ethidium bromide was confirmed by a continuous fluorescence assay.

scholarly journals Extraction model of the longitudinal flagellum of Ceratium tripos (Dinoflagellida): reactivation of flagellar retraction

1985 ◽  
Vol 75 (1) ◽  
pp. 313-328
Author(s):  
T. Maruyama
Keyword(s):  
Ionic Strength ◽  
Sodium Glutamate ◽  
Glass Capillary ◽  
Atpase Inhibitor ◽  
Nitrobenzoic Acid ◽  
Low Ionic Strength ◽  
Triton X ◽  
Extraction Model ◽  
Hydrolysis Of ◽  
Strength Medium

The mechanism of retraction of the longitudinal flagellum of Ceratium tripos was studied by making extracted models of the flagellum. Non-detergent models extracted in low ionic strength medium containing 1 M-glucose, 10 mM-EDTA, and 50 mM-Tris X HCl buffer (pH 8.0), retracted when Ca2+, Mg2+, Ba2+, Sr2+, Mn2+ or Cd2+ was applied locally with a glass capillary. A demembranated model of the flagellum was made with an extraction medium containing 0.8-1.0 M-glucose, 20 mM-Tris-acetate (pH 7.8), 2 mM-EGTA, 5–7 mM-MgSO4, 0.1 M-potassium glutamate and 0.1% Triton X-100. The model required a concentration of Mg2+ of a few mmol/l for successful reactivation of both retraction and undulation, and about 0.1 M-potassium glutamate (or sodium glutamate) for reactivation of undulation. Neither type of motion of the models could be reactivated above 35 degrees C. Ca2+ induced the retraction at pCa 5.5 or less. In addition to Ca2+, Mn2+, Ba2+, Sr2+ and Cd2+ also induced retraction but Mg2+, La3+ or Tb3+ did not. Although ATP was required for undulation, it was not required for retraction. Co-incubation with hexokinase to remove contaminating ATP did not suppress the retraction. The potent ATPase inhibitor, orthovanadate, inhibited undulation at 10 micron but did not inhibit retraction even at 2 mM. SH blockers, N-ethylmaleimide and dithio-bis-nitrobenzoic acid strongly suppressed undulation but had no effect on retraction. Calmodulin inhibitors, trifluoperazine and chlorpromazine, also had no effect on retraction. These data indicate that undulation is generated by a 9 + 2 microtubular axoneme using energy released by hydrolysis of ATP and that retraction can be induced by Ca2+ without a requirement for ATP.

scholarly journals Inhibition of friend murine leukemia virus production by low-ionic-strength medium.

1976 ◽  
Vol 17 (2) ◽  
pp. 453-461 ◽  
Author(s):  
M A Ussery ◽  
R Ramirez-Mitchell ◽  
B A Hardesty
Keyword(s):  
Ionic Strength ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Production ◽  
Low Ionic Strength ◽  
Strength Medium ◽  
Murine Leukemia
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