Studies on the Japanese pickles Sunki. Part X Sunki-pickles prepared by single species of lactic acid bacteria.

The cellular fatty acid composition of about 160 strains of Bacillus, lactic acid bacteria, Enterobacteriaceae, and Staphylococcus was analyzed in order to identify foodborne bacteria. In a first step, the reproducibility of the procedure, from the bacterial growth to the extraction and analysis conditions, was evaluated. The stability of fatty acid composition under controlled conditions was confirmed through high similarity levels (So ≥ 94). Secondly, intraspecies similarity was estimated. The existence of low values (So ≤ 79) was demonstrated within each of the four groups of strains investigated, which stressed the importance of analyzing a high number of reference strains in each species. The intraspecies similarity was maximal under optimal growth conditions. Finally, interspecies similarity was compared to intraspecies similarity. The best results of discrimination between species was obtained for the heterogeneous genus Bacillus, for which the 12 species studied could be separated into 6 clusters at a similarity level of 79. The association of 27 species of lactic acid bacteria into 8 clusters should be useful to confirm results of other characterization methods. Only 3 clusters were formed for the 16 Enterobacteriaceae species studied. The genus Salmonella could not be discriminated. The 10 Staphylococcus species were separated into 5 clusters. Coagulase-positive and -negative strains were not differentiated. Since the fatty acid composition did not usually appear specific to single species, characterization of foodborne bacteria by the analysis of their cellular fatty acids should be used to complement other taxonomic methods.

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207. Protein metabolism and acid production by the lactic acid bacteria in milk. Influence of yeast extract and chalk

Journal of Dairy Research ◽

10.1017/s0022029900002685 ◽

1939 ◽

Vol 10 (1) ◽

pp. 20-34 ◽

Cited By ~ 9

Author(s):

M. Braz ◽

L. A. Allen

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Proteolytic Activity ◽

Yeast Extract ◽

Protein Metabolism ◽

Acid Production ◽

Single Species ◽

Soluble Nitrogen ◽

Protein Breakdown ◽

Protein Nitrogen

Though the lactic acid bacteria are recognized primarily as saccharolytic, several workers have recorded observations on their slow proteolytic activity. Von Freudenreich(1) was the first to record the fact that cultures of these organisms in milk, to which chalk had been added to neutralize the acidity, formed appreciable amounts of soluble nitrogen, and these findings were confirmed by Orla-Jensen (2), Barthel(3), and Barthel & Sandberg(4). Anderegg & Hammer (5), in a study of a large number of strains ofStr. Lactis, found an increase in soluble nitrogen in some cases and a decrease in others, while occasionally the same strain differed in different tests. In general, cultures which clotted rapidly were more inclined to proteolysis than those which were slower in forming acid.Str. citrovorusandStr. paracitrovorusdid not cause protein breakdown. Addition of 0·3% peptone to the milk tended to retard proteolysis or to increase negative values while addition of chalk resulted in more extensive proteolysis. Barthel & Sadler (6) found that starters consisting of mixed cultures of streptococci produced more extensive proteolysis than single species, indicating a symbiotic effect. Sherwood & Whitehead (7) tested the proteolytic powers of several strains ofStr. cremorisin chalk milk cultures and found some active and some comparatively inactive. Two strains appear to have formed surprisingly large amounts of non-protein nitrogen. In general they found that acid-producing power was linked with proteolytic power.

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Application of mixed starter culture for table olive production

Grasas y Aceites ◽

10.3989/gya.0220201 ◽

2021 ◽

Vol 72 (2) ◽

pp. e405

Author(s):

Z.Ş. Erdemir Tıraş ◽

H. Kalkan Yıldırım

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Starter Culture ◽

Single Species ◽

Starter Cultures ◽

Table Olives ◽

Spontaneous Fermentation ◽

Natural Fermentation ◽

Table Olive ◽

Natural Microbiota

The fermentation of olives is usually carried out spontaneously by natural microbiota. Spontaneous fermentation has some disadvantages, such as the formation of defects in the end product due to the activities of undesirable microorganisms. The use of starter cultures could be a promising option to provide a more controlled fermentation environment and to reduce the risk of spoilage. Mixed starter culture use (generally selected Lactobacillus strains with or without yeasts) could reduce pH in a shorter time, producing a higher amount of lactic acid and enhancing microbial safety compared to fermentation with starter cultures containing single species or natural fermentation. Their use could also enhance the organoleptical properties of table olives. Particularly the use of yeast (such as strains of W. anomolus, S. cerevisiae) in the fermentation of olives, in combination or sequentially with lactic acid bacteria could result in an increase in volatile compounds and a more aromatic final product.

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Microbiome: Structures from Lactic Acid Bacteria

PSI Structural Genomics Knowledgebase ◽

10.1038/sbkb.2012.159 ◽

2013 ◽

Author(s):

Arianne Heinrichs

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria

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Lactic acid bacteria and yeasts in kefir grains and kefir made from them

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.7000186 ◽

2002 ◽

Vol 28 (1) ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

E Simova ◽

D Beshkova ◽

A Angelov ◽

Ts Hristozova ◽

G Frengova ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Kefir Grains

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Method of separation of major off-odor component and change of components of Gastrodia elata Blume fermented by lactic acid bacteria

10.1055/s-0037-1608346 ◽

2017 ◽

Author(s):

S Young Eun ◽

L In Sok ◽

S Eun Ju ◽

C Min Kyung ◽

H Hyu Ah ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Gastrodia Elata ◽

Gastrodia Elata Blume

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Development of biotechnology for gluten-free sourdough bakery products with a new microbial composition

Khleboproducty ◽

10.32462/0235-2508-2020-29-12-59-63 ◽

2020 ◽

Vol 29 (12) ◽

pp. 59-63

Author(s):

O.I. Parakhina ◽

◽

M.N. Lokachuk ◽

L.I. Kuznetsova ◽

E.N. Pavlovskaya ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Starter Culture ◽

Comparative Assessment ◽

Starter Cultures ◽

Bakery Products ◽

Gluten Free ◽

Microbial Composition ◽

Fermentative Activity ◽

Theoretical Foundations

The research was carried out within the framework of the theme of state assignment № 0593–2019–0008 «To develop theoretical foundations for creating composite mixtures for bakery products using physical methods of exposure that ensure homogeneity, stability of mixtures and bioavailability of nutrients, to optimize diets population of Russia». The data on the species belonging of new strains of lactic acid bacteria and yeast isolated from samples of good quality gluten-free starter cultures are presented. A comparative assessment of the antagonistic and acid-forming activity of strains of lactic acid bacteria and the fermentative activity of yeast was carried out. The composition of microbial compositions from selected strains of LAB and yeast was developed. The influence of the starter culture on the new microbial composition on the physicochemical, organoleptic indicators of the bread quality and resistance to mold and ropy-disease was investigated.

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