A preliminary molecular assessment of the taxonomic validity of Hexagenia orlando Traver, 1931 (Ephemeroptera: Ephemeridae)

In an effort to evaluate the taxonomic uncertainty of the species status of Hexagenia orlando Traver, 1931 (Ephemeroptera: Ephemeridae), molecular data were used for the first time to test its current classification. Mitochondrial cytochrome c oxidase subunit I (COI) haplotypes were evaluated using three types of analyses in the form of distance-based, tree-based, and model-based delimitation methods. All analyses consistently recovered H. orlando as a valid species. These preliminary results lend evidence reinforcing the current classification scheme and encourage renewed scrutiny of adults and nymphs to hopefully identify additional morphological characters that may serve to separate H. orlando and its congener Hexagenia limbata (Serville, 1829).

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Taxonomy of the Sylvilagus brasiliensis complex in Central and South America (Lagomorpha: Leporidae)

Journal of Mammalogy ◽

10.1093/jmammal/gyz126 ◽

2019 ◽

Vol 100 (5) ◽

pp. 1599-1630 ◽

Cited By ~ 1

Author(s):

Luis A Ruedas ◽

Sofia Marques Silva ◽

Johnnie H French ◽

Roy Nelson Platt ◽

Jorge Salazar-Bravo ◽

...

Keyword(s):

South America ◽

Morphological Characters ◽

Molecular Data ◽

Type Material ◽

Valid Species ◽

South American ◽

Species Status ◽

Neotropical Species ◽

Nomen Dubium ◽

Taxonomic Framework

Abstract A taxonomic framework for South American cottontail rabbits (Lagomorpha: Leporidae: Sylvilagus) was recently published by Diersing and Wilson (2017). Although we agree with some of its taxonomic conclusions (e.g., species status for S. apollinaris and S. fulvescens), we disagree with others. We provide herein evidence supporting S. andinus as a valid species based on morphological characters and novel molecular data. We also provide details of the morphological characters of S. apollinaris and S. fulvescens that support separating these from S. brasiliensis. We adduce data suggestive to the effect that—absent any type material—S. defilippi is at best a nomen dubium. Finally, we provide evidence in support of recognizing additional Neotropical species of Sylvilagus. Un esquema taxonómico para los conejos sudamericanos (Lagomorpha: Leporidae: Sylvilagus) fue recientemente publicado por Diersing y Wilson (2017). Aunque estamos de acuerdo con algunas de sus conclusiones (por ejemplo: estatus de especie válida para S. apollinaris y S. fulvescens), no estamos de acuerdo con las restantes conclusiones taxonómicas. Aportamos aquí pruebas convincentes sobre la característica naturaleza de los caracteres morfológicos y moleculares de S. andinus, pruebas que esgrimimos en apoyo de la hipótesis que esta última es una especie válida, así confirmando su escisión de S. brasiliensis. Proporcionamos detalles de los caracteres morfológicos de S. apollinaris y S. fulvescens que confirman la decisión taxonómica de asimismo separarlos de S. brasiliensis. Proporcionamos datos en aditamento que indican que a falta de cualquier material tipo para S. defilippi, este nombre es en el mejor de los casos un nomen dubium. Finalmente, ofrecemos datos y evidencia apoyando nuestras decisiones de reconocer un mayor número de especies Neotropicales de Sylvilagus que previamente se conocían.

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Amanita subjunquillea and its ectomycorrhizal association, reported as new for Pakistan

Mycotaxon ◽

10.5248/134.413 ◽

2019 ◽

Vol 134 (3) ◽

pp. 413-423

Author(s):

Muhammad Ishaq ◽

Arooj Naseer ◽

Munazza Kiran ◽

Muhammad Fiaz ◽

Abdul Nasir Khalid

Keyword(s):

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Sequence Analyses ◽

Dark Orange ◽

Ectomycorrhizal Roots ◽

Morphological And Molecular Data ◽

Quercus Floribunda ◽

First Time ◽

Ectomycorrhizal Association

Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characters and nucleotide sequence analyses of the ITS region generated from basidiomata and ectomycorrhizal roots of Quercus floribunda. Our collection differs from the type in its dark orange pileus disc and pale yellow margins. Remaining morphological and molecular data are consistent with previously reported specimens. This represents the first report of A. subjunquillea from Pakistan.

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Male identification, generic classification and sexual dimorphism of Micronychus pardus (Kazantsev, 2018) comb. nov. (Coleoptera: Lycidae: Calochrominae)

Zootaxa ◽

10.11646/zootaxa.4657.1.9 ◽

2019 ◽

Vol 4657 (1) ◽

pp. 177-182 ◽

Cited By ~ 2

Author(s):

MICHAL MOTYKA

Keyword(s):

Mitochondrial Dna ◽

Sexual Dimorphism ◽

Species Identification ◽

Dna Sequences ◽

Morphological Characters ◽

Molecular Data ◽

Large Specimen ◽

Molecular Analyses ◽

Almost All ◽

First Time

Almost all net-winged beetles are members of Müllerian complexes and their similarity due to phenotypic coevolution sometimes complicates species identification and generic placement. Therefore, large specimen series, detailed exhaustive examination of morphological characters and molecular data are needed to clarify the taxonomic placement. Using mitochondrial DNA sequences, I investigated the sexual dimorphism and generic placement of the recently described species Calochromus pardus Kazantsev, 2018. I found that the species does not belong in Calochromus Guérin-Méneville, 1833 and all morphological characters and molecular analyses point to its placement in Micronychus Motschulsky, 1861. Therefore, Micronychus pardus (Kazantsev, 2018), comb. nov. is proposed. Additionally, the male is described here for the first time showing the sexual dimorphism in the species. Unlike the females, the males do not superficially resemble members of Xylobanus Waterhouse, 1879 with bright coloured elytral costae and black background, but mimics the sympatrically occurring yellow and black lycids in the genus Cautires Waterhouse, 1879.

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Studies on Longidorus iberis (Escuer & Arias, 1997) n. comb. (Nematoda: Longidoridae) from Spain

Nematology ◽

10.1163/15685411-bja10082 ◽

2021 ◽

pp. 1-15

Author(s):

Ilenia Clavero-Camacho ◽

Gracia Liébanas ◽

Miguel Escuer ◽

Carolina Cantalapiedra-Navarrete ◽

Antonio Archidona-Yuste ◽

...

Keyword(s):

Molecular Markers ◽

18S Rrna ◽

Molecular Data ◽

Valid Species ◽

28S Rrna ◽

Closely Related Species ◽

North West ◽

Molecular Studies ◽

First Time ◽

Partial 18S

Summary Specimens of a thin longidorid species collected in Peñalba (Huesca), north-west Spain, were previously described as Paralongidorus iberis. However, we conclude, through scanning electron microscopy and molecular studies on a population from about 15 km from the type locality and on paratype specimens, that this species was originally placed in the wrong genus. Both populations have pore-like amphidial apertures, not slit-like as in Paralongidorus, and the species is therefore transferred to Longidorus. Longidorus iberis n. comb. is regarded as a valid species and is clearly different from closely related species such as L. tabernensis, L. iliturgiensis, L. alvegus and L. indalus in morphometrics and molecular markers. Molecular data are reported for the first time, including the D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, partial 18S rRNA and partial mitochondrial coxI regions. These molecular markers were used for inferring the phylogenetic relationships with other species within Longidorus and Paralongidorus, all clearly separating L. iberis n. comb. from other related taxa and placing the species in the Longidorus clade, rather than with Paralongidorus.

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The Conopidae of Costa Rica (Diptera) (Part 1: Conopinae – Conopini &Tropidomyiini)

Zootaxa ◽

10.11646/zootaxa.1528.1.1 ◽

2007 ◽

Vol 1528 (1) ◽

pp. 1-40

Author(s):

JENS-HERMANN STUKE ◽

JEFFREY H. SKEVINGTON

Keyword(s):

Costa Rica ◽

Junior Synonym ◽

Valid Species ◽

Species Status ◽

Taxonomic Changes ◽

First Time

The first part of a Conopid faunal review for Costa Rica presents data for 25 species from the Conopinae (tribes Tropidomyiini and Conopini). Two hundred and eighty-five specimens have been examined and identified. Physocephala herrerai spec. nov. and Physoconops zumbadoi spec. nov. are new to science. Sixteen species are reported the first time for the country. We introduce the following taxonomic changes: Physoconops (Gyroconops) ocellatus (Giglio-Tos, 1892) is a valid species [status rev.]; Physoconops rufipennis (Macquart, 1844) belongs to the subgenus Pachyconops Camras, 1955 [status rev.]; Physoconops gracilianus Camras, 1955 is a junior synonym of Physoconops pallifrons (Coquillett, 1904) [syn. nov.]. Keys for the species of the genera Physocephala Schiner, 1861 and Physoconops Szilady, 1926 are presented. Most species are illustrated with photo plates.

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Phylogenetic analysis and taxonomy of the subgenus Gnathopaederus Chapin and related groups of Paederus s.l. (Coleoptera:Staphylinidae:Paederinae)

Invertebrate Systematics ◽

10.1071/is09015 ◽

2009 ◽

Vol 23 (5) ◽

pp. 422

Author(s):

Xiao-Yan Li ◽

Hong-Zhang Zhou

Keyword(s):

Phylogenetic Analysis ◽

Type Species ◽

Morphological Characters ◽

Character State ◽

Robust Solution ◽

Current Classification ◽

The Status ◽

Definition Of ◽

Taxonomic Studies ◽

First Time

The paederine genus Paederus s.l. (Coleoptera : Staphylinidae) is a hyperdiverse group of some 500 species worldwide. Although the subtribe Paederina, in which the genus resides, is monophyletic the status of numerous genera and subgenera is questionable and the current classification is likely to be flawed. As part of detailed studies on the paederine fauna of China, we undertook a preliminary morphologically-based phylogenetic analysis to determine the relationships of Paederus (Gnathopaederus) and related subgenera, as a basis for a revision of the subgenus, and as an attempt to provide a better framework for future taxonomic studies on the group as a whole. The analysis was based on 66 adult morphological characters for 30 ingroup taxa, including all six species of P. (Gnathopaederus). The results show that P. (Gnathopaederus) and P. (Paederus), and the genera Megalopaederus, Pachypaederus and Paederidus are monophyletic, and that P. (Eopaederus) and P. (Harpopaederus) are paraphylyetic. However, our analysis did not offer a robust solution to relationships among other clades within Paederus s.l. although it seems clear that Paederus s.l. is not monophyletic with respect to the above genera. The monophyly of P. (Gnathopaederus) is supported by the mandibular basal medial tooth having two cuspids separated dorsoventrally. Based largely on this character state, the definition of the subgenus is refined; six species are recognized, including two new species described from China as follows: P. (G.) daozhenensis, sp. nov. from Guizhou, and P. (G.) jilongensis, sp. nov. from Xizang; three species, P. bursavacua Willers, P. yunnanensis Willers and P. solodovnikovi Willers, are newly assigned to the subgenus according to a revised subgeneric definition. New morphological characters including important secondary sexual characters and sexual dimorphism are described and male genitalia are illustrated for the first time for the type species of the subgenus, P. (G.) szechuanus (Chapin). A key to the six species is presented and the distribution of each species is documented and discussed.

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A revision of the Trimeresurus puniceus-complex (Serpentes: Viperidae: Crotalinae) based on morphological and molecular data

Zootaxa ◽

10.11646/zootaxa.1293.1.1 ◽

2006 ◽

Vol 1293 (1) ◽

pp. 1 ◽

Cited By ~ 2

Author(s):

PATRICK DAVID ◽

GERNOT VOGEL ◽

S. P. VIJAYAKUMAR ◽

NICOLAS VIDAL

Keyword(s):

Species Concept ◽

Distinct Species ◽

Morphological Characters ◽

Molecular Data ◽

Biological Species ◽

Valid Species ◽

Biological Species Concept ◽

Phylogenetic Species Concept ◽

Morphological And Molecular Data ◽

A New Species

The brown Asian pitvipers of the genus Trimeresurus related to Trimeresurus puniceus (informal Trimeresurus puniceus-complex) are revised on the basis of morphological and molecular analyses. Variation in morphological characters were investigated among 119 specimens from 62 populations of the whole range of the pitvipers currently known as Trimeresurus puniceus (Boie, 1827), Trimeresurus borneensis (Peters, 1872) and Trimeresurus brongersmai Hoge, 1969. Molecular and morphological analyses clearly differentiate two groups of taxa, referrable to the informal Trimeresurus puniceus-group and Trimeresurus borneensis-group, and confirm the distinct specific status of T. puniceus and T. borneensis. Morphological univariate and multivariate analyses differentiate six clusters of populations that are morphologically diagnosable, of which five are here considered to represent independent lineages and one is placed incertae sedis pending the availability of further specimens. These clusters are considered to be distinct species following the Biological Species Concept and the Phylogenetic Species Concept. One of them is described as a new species, Trimeresurus andalasensis spec. nov. (T. borneensis-group), which includes populations from northern Sumatra. Trimeresurus wiroti Trutnau, 1981 is revalidated to accommodate populations from Thailand and West Malaysia. Trimeresurus borneensis is here considered endemic to Borneo. Trimeresurus puniceus is known from Java and from South Sumatra, but the taxonomy of this species in Sumatra is left unresolved. Also left unresolved is the taxonomic position of specimens from western Sumatra and the Mentawai Archipelago, and from the Natuna Islands and Anamba Islands. Although belonging to the T. puniceus-group, they show some differences to other specimens of the group. They are not referred to any taxon pending the collection of additional specimens. Lastly, Trimeresurus brongersmai is confirmed as a valid species from the Mentawai Archipelago. A key to these taxa is provided.

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Anopheles (Kerteszia) lepidotus (Diptera: Culicidae), not the malaria vector we thought it was: Revised male and female morphology; larva, pupa, and male genitalia characters; and molecular verification

Zootaxa ◽

10.11646/zootaxa.3218.1.1 ◽

2012 ◽

Vol 3218 (1) ◽

pp. 1 ◽

Cited By ~ 5

Author(s):

BRUCE A. HARRISON ◽

FREDDY RUIZ-LOPEZ ◽

GUILLERMO CALDERON FALERO ◽

HARRY M. SAVAGE ◽

JAMES E. PECOR ◽

...

Keyword(s):

Malaria Vector ◽

Pupal Stage ◽

Morphological Characters ◽

Molecular Data ◽

Coi Gene ◽

Larval Stages ◽

Female Paratypes ◽

Rdna Its2 ◽

The Andes ◽

First Time

The name Anopheles (Kerteszia) lepidotus Zavortink, commonly used for an important malaria vector in the eastern cor-dillera of the Andes, is here corrected to An. pholidotus Zavortink. We discovered that An. (Ker.) specimens from Peru,and reared-associated specimens from Ecuador, had unambiguous habitus characters that matched those on the male ho-lotype of An. lepidotus. However, the specimens do not exhibit characters of the female allotype and female paratypes ofAn. lepidotus, which are actually An. pholidotus. Our specimens are the first correctly associated females of An. lepidotus,which allow us to provide a new morphological interpretation for the adult habitus of this species. This finding is alsocorroborated by molecular data from a portion of the Cytochrome Oxidase I (COI) gene and ribosomal DNA Internal Tran-scribed Spacer 2 (rDNA ITS2). The pupal stage of An. lepidotus is described for the first time, and additional larval char-acters are also noted. Diagnostic morphological characters for the adult, pupal, and larval stages of An. pholidotus areprovided to separate the two species. All stages of An. lepidotus are easily separated from other currently known speciesin subgenus Kerteszia and a new key to the females of An. (Kerteszia) is given. Previously published distribution, bionomics, and medical significance data are corrected and enhanced.

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Revision of the genus Aspicera Dahlbom, 1842 (Hym.: Figitidae: Aspicerinae)

Zootaxa ◽

10.11646/zootaxa.3606.1.1 ◽

2013 ◽

Vol 3606 (1) ◽

pp. 1-110 ◽

Cited By ~ 8

Author(s):

P. ROS-FARRÉ ◽

J. PUJADE-VILLAR

Keyword(s):

New Species ◽

Species Diversity ◽

Morphological Characters ◽

Type Material ◽

Valid Species ◽

General Lack ◽

Incertae Sedis ◽

Two New Species ◽

First Time ◽

Lack Of Knowledge

The genus Aspicera Dahlbom (Hymenoptera: Cynipoidea: Figitidae: Aspicerinae) is revised herein. Aspicera has a Holarctic distribution, being here cited for the first time from the following countries: Canada, Cyprus, Greece, India (northeastern corner, which is part of the Himalayan southeastern range and is considered as belonging to the Palaearctic), Japan, Jordan, Mexico, Montenegro, and Turkey. Morphological characters necessary to differentiate the species of Aspicera are described. The 27 previously described species of Aspicera were revised, always studying the type material when available (21 species); the type material of A. aegyptica Hedicke, 1928, A. chlapowskii Kieffer, 1901, A. coriacea Kieffer, 1901, A. lobata Hedicke, 1928, A. sibirica Kieffer, 1901, A. spinosa (Boyer de Fonscolombe, 1832) are lost or destroyed, these species were studied with the original descriptions. Of the 27 known species, 16 are considered as valid and are redescribed; A. effincta Belizin, 1952 is syn. nov. of A. suecica Dalla Torre & Kieffer, 1910, and A. brevispina Kieffer, 1901 and A. coriacia Kieffer, 1901 are syn. nov. of A. hartigi Dalla Torre, 1889. Aspicera nigra Ionescu, 1969, A. nigricornis Kirby, 1889 and A. rugosa (Hartig, 1843) do not belong to the Aspicerinae, but respectively to the genera Xyalophora and Neralsia (Figitinae), and Xyalaspis (Anacharitinae): Xyalophora nigra (Ionescu) n. comb., Neralsia nigricornis (Kirby) n. comb. and Xyalaspis rugosa Hartig status restored. Aspicera lobata Hedicke, 1928 is considered as ‘incertae sedis’. The examination of additional specimens suggests that there is a general lack of knowledge of the species diversity of this genus. Thirty two new species are described: A. adelae Ros-Farré n. sp., A. annae Ros-Farré & Pujade-Villar n. sp., A. belizini Ros-Farré & Pujade-Villar n. sp., A. blancae Ros-Farré n. sp., A. buffingtoni Ros-Farré & Pujade-Villar n. sp., A. caminali Ros-Farré n. sp., A. carinata Ros-Farré & Pujade-Villar n. sp., A. dianae Ros-Farré n. sp., A. kovalevi Ros-Farré & Pujade-Villar n. sp., A. elisendae Ros-Farré n. sp., A. forshzarai Pujade-Villar & Ros-Farré n. sp., A. gemmae Ros-Farré & Pujade-Villar n. sp., A. jantonii Ros-Farré n. sp., A. julii Ros-Farré & Pujade-Villar n. sp., A. kiefferi Ros-Farré & Pujade-Villar n. sp., A. magdae Ros-Farré & Pujade-Villar n. sp., A. marginata Ros-Farré & Pujade-Villar n. sp., A. martae Ros-Farré & Pujade-Villar n. sp., A. danielssoni Ros-Farré & Pujade-Villar n. sp., A. carlestolrai Ros-Farré & Pujade-Villar n. sp., A. mireiae Ros-Farré & Pujade-Villar n. sp., A. porif Ros-Farré n. sp., A. marginata Ros-Farré & Pujade-Villar n. sp., A. punctifrons Ros-Farré & Pujade-Villar n. sp., A. readae Ros-Farré & Pujade-Villar n. sp., A. robusta Ros-Farré & Pujade-Villar n. sp., A. santamariai Ros-Farré & Pujade-Villar n. sp., A. sergioi Ros-Farré n. sp., A. singularica Ros-Farré & Pujade-Villar n. sp., A. teresae Ros-Farré n. sp., A. tomasi Ros-Farré n. sp., A. zuparcoi Ros-Farré & Pujade-Villar n. sp. A key to all 48 valid species of Aspicera is given. All species are illustrated.

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Genetic relationship and identification of Dioscorea polystachya cultivars accessed by ISAP and SCAR markers

Archives of Biological Sciences ◽

10.2298/abs150717098p ◽

2017 ◽

Vol 69 (2) ◽

pp. 277-284 ◽

Cited By ~ 4

Author(s):

Bin Peng ◽

Yanmei Zhang ◽

Xiaoqin Sun ◽

Mimi Li ◽

Jiayu Xue ◽

...

Keyword(s):

Genetic Relationships ◽

Single Species ◽

Morphological Characters ◽

Molecular Data ◽

Breeding Programs ◽

Sequence Characterized Amplified Region ◽

Conservation Actions ◽

High Level ◽

First Time

A survey of intron sequence amplified polymorphism (ISAP) in conjunction with sequence characterized amplified region (SCAR) was carried out to examine the genetic relationships among 14 Dioscorea polystachya cultivars and identify the most popular cultivar ?Tiegun?. Our results revealed that there is a high level of polymorphism among these cultivars. Furthermore, in this study, ISAP markers were consistent with the morphological characters of Dioscorea polystachya cultivars and previous hypotheses on the classification of these cultivars into 2 groups via leaf and tuber shapes have been confirmed. Based on morphological characters and molecular data, we show for the first time that D. doryphora might be a single species and another progenitor of these cultivars. An ISAP fragment specific to the ?Tiegun? cultivar was converted into a SCAR marker. This marker could be used to discriminate the ?Tiegun? cultivar from the other 13 cultivars. Overall, the results of our study provide the foundations for subsequent breeding programs or conservation actions.

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