scholarly journals Coagulation and fibrinolytic potential of blood plasma in patients with stage VD CKD, treated by program hemodialysis, as the markers of thrombotic risk

2018 ◽  
Vol 22 (3) ◽  
pp. 494-497
Author(s):  
O.B. Storozhuk ◽  
I.B. Seleznyova ◽  
L.O. Storozhuk ◽  
B.G Storozhuk ◽  
T.V. Dovgalyuk ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Statistical Processing ◽  
High Concentration ◽  
Thrombotic Risk ◽  
C Protein ◽  
Soluble Fibrin ◽  
Thrombotic Complications ◽  
Fibrinolytic Potential ◽  
Protein C Activity ◽  
D Dimer

Thrombotic complications, associated with hemostatic system disturbances in patients with stage VD chronic kidney disease (CKD), occur rather frequently. Hence, the search of early available biological markers in diagnosis of this complication is of great significance. Objective – to study coagulation potential of blood plasma in patients with stage VD CKD, treated by program hemodialysis, and to determine the markers of thrombotic risk. 88 patients (aged 26–65) with stage VD CKD, treated by program hemodialysis, were studied. Hemostatic potential of blood was determined by spectrophotometry; soluble fibrin level — by two-site enzyme-linked immune-sorbent quantitative assay; D-dimer — by enzyme immunoassay using monoclonal antibodies to D-dimer epitopes; protein C activity — by spectrophotometry with wavelength 405 nm; fibrinogen contents — by spectrophotometry with wavelength 280 nm. Statistical processing was performed by methods of variation statistics and correlation analysis. All patients were found to have significant increase of soluble fibrin level (4.03±0.15÷4.62±0.31 mg/ml) (р <0.001), but there was a group of patients (n=25), who demonstrated increased coagulation potential, (р <0.001), significantly prolonged fibrinolysis (р <0.001) and increased soluble fibrin concentration (5.36±0.61÷5.78±0.82 µg/ml) (р <0.001) along with the tendency to decreased C protein level and no response of D-dimer to increased soluble fibrin level. Detected changes in coagulation properties of blood plasma can serve the markers of thrombotic risk. Thus, the patients with stage VD CKD, treated by program hemodialysis, have high risk of thrombosis, associated with high concentration of soluble fibrin and fibrinogen. But in this category of patients, rather low fibrinolytic potential, as well as the level of natural anticoagulant — C protein — is observed. The studied homeostatic parameters can be used as the markers of thrombotic risk.

scholarly journals Possibilities of using hemostasis markers and parameters of hemostatic potential for predicting thrombotic complications in patients with CKD stage VD

2019 ◽  
Vol 23 (4) ◽  
pp. 677-680
Author(s):  
O.B. Storozhuk ◽  
S.V. Shevchuk ◽  
I.B. Seleznyova ◽  
L.O. Storozhuk ◽  
T.V. Dovgalyuk ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Enzyme Immunoassay ◽  
Thrombotic Risk ◽  
Hemostatic System ◽  
Prophylactic Measures ◽  
Ckd Stage ◽  
Thrombotic Complications ◽  
Fibrinolytic Potential ◽  
D Dimer

Annotation. Violations in the hemostatic system of patients with chronic kidney disease (CKD) of the VD stage treated with program hemodialysis are associated with a high thrombotic risk. Comprehensive studies of the possible causes and predictors of thrombophilia can improve the therapeutic and prophylactic measures to combat thrombotic complications. The search for available methods for predicting these complications is very relevant. The goal is to develop a method for predicting the development of thrombotic complications in this category of patients, which would allow identifying the degree of hemostasis disorders. We examined 88 patients with CKD VD, treated with program hemodialysis, who determined the fibrinolytic potential and the level of soluble fibrin and D-dimer by enzyme immunoassay. It is proposed to use the developed fibrinolytic coefficient (Fc) to predict thrombotic complications. It was found that the proposed method for assessing hemostasis using Fc makes it possible to objectively characterize the state of the coagulation link and can serve as an indicator of thrombotic risk.

scholarly journals Some indicators of hemostasis and mortality from thrombotic complications in patients with CKD stage VD who were treated by programmed hemodialysis (five-year follow-up)

2021 ◽  
Vol 25 (3) ◽  
pp. 409-412
Author(s):  
O. B. Storozhuk ◽  
I. B. Seleznyova ◽  
L. O. Storozhuk ◽  
T. M. Platonova ◽  
B. G. Storozhuk ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Protein C ◽  
Control Group ◽  
Strong Positive Correlation ◽  
General Group ◽  
Soluble Fibrin ◽  
Ckd Stage ◽  
Thrombotic Complications ◽  
D Dimer

Annotation. Thrombotic complications in patients with stage VD chronic kidney disease (CKD), treated by program hemodialysis is one of the causes of high mortality in this category of patients; its major pathogenetic component proved to be hemostatic system disturbances characterized by systemic activation of blood clotting process leading to the development of thrombophilia. Objective – to study the biochemical molecular markers of hemostasis in patients with stage VD CKD, treated by program hemodialysis, and to determine their long-term effect on the development of thrombotic complications and mortality. The study included 88 patients (52 males and 36 females) aged 26 to 65 years with stage VD CKD, treated by program hemodialysis. The patients were followed up for five years. Soluble fibrin (sF) level was determined by two-site enzyme-linked immune-sorbent quantitative assay; D-dimer – by enzyme immunoassay using specific monoclonal antibodies to D-dimer epitopes; protein C (pC) activity in blood plasma was estimated by its activation with copperhead snake venom followed by spectrophotometry with wavelength 405 nm. Blood plasma fibrinogen was determined using thrombin-like enzyme Antsistron-H by spectrophotometry with wavelength 280 nm. Blood plasma fibrinolytic activity was evaluated by the relationship between D-dimer and soluble fibrin. Processing of materials was carried out using the methods of variation statistics and correlation analysis. During five years of follow up there were 13 deaths (14.8%), 7 among males and 6 among females, caused by thrombotic complications. The main thrombotic complications were myocardial infarction (6), ischemic stroke (4), mesenteric thrombosis (2) and disseminated intravascular coagulation syndrome (DIC) (1) (according to pathomorphological data). The patients of general group were found to have significantly increased sF level, decreased pC as compared to the control group, as well as twofold increase of fibrinogen concentration along with decreased D-dimer/sF ratio and no response of D-dimer to increased soluble fibrin level. The tendency of D-dimer/sF ratio to increase in those who died because of thrombotic complications could be indicative of microthrombosis with formation of fibrin derivatives along with mild activation of fibrinolysis. Correlation relationships between soluble fibrin and D-dimer, fibrinogen and protein C in general group were assessed, and the following data were obtained: medium direct relationship between soluble fibrin and D-dimer (r= 0.56) (p<0.001), and absence of correlation with fibrinogen (r= -0.12) and protein C (r= -0.10) (p˃0.1). Besides, strong positive correlation was demonstrated between D-dimer and soluble fibrin (r= 0.87) (p<0.001), and moderately negative one between D-dimer and protein C (r= -0.21) (p<0.05). It should be noted that in patients with thrombotic complications, positive correlations between soluble fibrin and D-dimer become stronger (r= 0.51) (p<0.001), as well as negative ones between soluble fibrin and protein C (r= -0.22) (p<0.05). Depressive state of anticoagulant component along with activation of coagulation factors are supposed to be one of the indicators predicting thrombophilia in this category of patients.

scholarly journals STATUS OF PRO- AND ANTICOAGULANT COMPONENTS OF HEMOSTASIS IN PATIENTS WITH STAGE VD CKD DEPENDING ON RESIDUAL RENAL FUNCTION

2020 ◽  
Vol 73 (8) ◽  
pp. 1723-1725
Author(s):  
Oleksiy B. Storozhuk ◽  
Iryna B. Seleznyova ◽  
Larysa A. Storozhuk ◽  
Tetyana V. Dovgalyuk ◽  
Borys G. Storozhuk ◽  
...  
Keyword(s):  
Renal Function ◽  
Protein C ◽  
Residual Renal Function ◽  
Positive Influence ◽  
Soluble Fibrin ◽  
Key Factor ◽  
The Status ◽  
Thrombotic Complications ◽  
Medical University ◽  
D Dimer

1RESEARCH INSTITUTE OF REHABILITATION OF NATIONAL PIROGOV MEMORIAL MEDICAL UNIVERSITY, VINNYTSIA, UKRAINE 2NATIONAL PIROGOV MEMORIAL MEDICAL UNIVERSITY, VINNYTSIA, UKRAINE 3PALLADIN INSTITUTE OF BIOCHEMISTRY OF NAS OF UKRAINE, KYIV, UKRAINE ABSTRACT The aim: To study the status of pro- and anticoagulant components of hemostasis in patients with stage VD CKD depending on residual renal function. Materials and methods: 88 patients with stage VD CKD were studied, 16 of them had preserved residual renal function (RRF). Hemostatic parameters – soluble fibrin, D-dimer and protein C – were determined in the patients. Results: Significant increase in soluble fibrin and fibrinogen levels along with depressive reaction of D-dimer and protein C were found in patients with stage VD CKD having lost RRF. Preserved RRF in those patients had positive influence on hemostatic profile, decreasing the degree of hypercoagulation. Conclusions: 1. In long term dialysis patients with stage VD CKD and lost RRF, significantly increased levels in soluble fibrin and fibrinogen along with decreased D-dimer concentration were found, being a key factor in the development of thrombotic complications. 2. There was significant decrease in protein C activity in patients with lost RRF. 3. Preserved RRF in patients with stage VD CKD had positive influence on hemostatic profile.

scholarly journals Influence of some comorbid and polymorbid states on the indicators of hemostasis in patients with ischemic heart disease and percutaneous angioplasty

2019 ◽  
Vol 23 (1) ◽  
pp. 134-137
Author(s):  
N.V. Storozhuk ◽  
T.M. Platonova ◽  
B.G. Storozhuk ◽  
E.V. Lugovskoy ◽  
L.A. Storozhuk ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Weighting Factor ◽  
Ischemic Heart ◽  
Comorbid Conditions ◽  
Percutaneous Angioplasty ◽  
Thrombotic Risk ◽  
C Protein ◽  
Medical Interventions ◽  
Thrombotic Complications

Identification of markers of possible thrombotic complications in patients with ischemic heart disease and percutaneous angioplasty with comorbid conditions on the background is significant for preventive medical interventions, depending on the degree of thrombotic risk. The hemostasis markers (sF, fibrinogen, D-dimer and C-protein) of 91 patients (77 men and 14 women) with ischemic heart disease and percutaneous angioplasty, of whom 32 patients had a stent retrombosis in anamnesis, were measured in different groups of comorbidity and polymorbidity. The purpose of the paper is to investigate changes in hemostasis in some comorbid conditions in patients with ischemic heart disease and percutaneous angioplasty, as well as to identify possible markers of thrombinemia. The results of the study suggest that the presence of comorbidity and polymorbidity in the investigated patients acts as a prognostic weighting factor in the development of thrombotic complications. A comprehensive study of hemostasis markers, among which sF is the most informative one, objectively characterizes disorders in the hemostasis system, while diabetes mellitus and restenosis in the anamnesis being the most dangerous comorbid conditions during the development of thrombotic complications.

scholarly journals Some indicators of hemostasis in patients with ischemic heart disease and coronary angioplasty

2018 ◽  
Vol 22 (3) ◽  
pp. 446-449
Author(s):  
N.V. Storozhuk ◽  
E.V. Luhovskoy ◽  
B.H Storozhuk ◽  
T.M. Platonova ◽  
I.M. Kolesnikov
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Coronary Angioplasty ◽  
Protein C ◽  
Ischemic Heart ◽  
Soluble Fibrin ◽  
Stent Restenosis ◽  
History Of ◽  
Thrombotic Complications ◽  
D Dimer

The effect of hemostasis parameters on the development of stent restenosis / thrombosis requires further investigation. The purpose of the paper is to find out the informativeness of activators and inhibitors of the circulating blood system as markers for the development of thrombotic complications in patients with ischemic heart disease and coronary angioplasty. 92 patients (78 men and 14 women) with ischemic heart disease and coronary angioplasty were examined, of which 33 cases suffered from stent restenosis/thrombosis. The patients were simultaneously evaluated for hemostasis parameters (soluble fibrin, D-dimer, protein C, and fibrinogen). It is determined that in patients with ischemic heart disease who have undergone angioplasty in 67.4% of cases there is a significant increase in the level of soluble fibrin with subsequent significant increase in patients with a history of restenosis. It is established that the level of D-dimer in these patients is significantly lower, while in the women it is significantly lower than that of the studied men. The reaction of anticoagulant protein C in 42.4% of patients tends to decrease, taking into consideration that in 80.4% cases there is significant fibrinogenemia. Therefore, the study reveals a significant imbalance in the system of pro- and anticoagulation in this category of patients in favor of the coagulation link, and the hemostatic parameters studied in the complex can be used as informative integral markers of thrombinemia.

scholarly journals Coagulopathy of Coronavirus Disease 2019: A Case Series

2021 ◽  
Vol 9 (B) ◽  
pp. 301-307
Author(s):  
Jonathan Arifputra ◽  
Harlinda Haroen ◽  
Linda Wilhelma Ancella Rotty ◽  
Pearla Fidei Cindy Lasut ◽  
Cecilia Hendratta
Keyword(s):  
Case Series ◽  
Low Molecular Weight ◽  
Not Given ◽  
Thrombotic Risk ◽  
Thromboembolic Risk ◽  
Risk Of Bleeding ◽  
Risk Assessment Models ◽  
Thrombotic Complications ◽  
D Dimer

BACKGROUND: In Coronavirus disease 2019 (COVID-19), the main manifestations were fever, cough, and anosmia. AIM: We aimed to investigate coagulopathy and disseminated intravascular coagulation in severe COVID-19 patients. METHODS: Five cases of COVID-19 with coagulopathy have been reported. RESULTS: All patients presented with various main complaints such as fever, cough, shortness of breath, and diarrhea. An increase in D-dimer value was found in all cases, with an increase of 4–5 times from the upper limit of normal. All patients were evaluated with the IMPROVE-VTE and PADUA thrombotic risk assessment models. Thromboprophylaxis with low molecular weight heparin LMWH intensive dosage was given to four patients. However, one patient was not given thromboprophylaxis because of the high risk of bleeding. All cases were not given long-term thromboprophylaxis after the patients were discharged from the hospital. All hospitalized COVID-19 patients can be considered to give thromboprophylaxis unless contraindicated. Thromboprophylaxis is preferable to use LMWH with a dose adjusted to the severity of COVID-19. Administration of thromboprophylaxis after hospitalization may be considered in patients who have thromboembolic risk factors. CONCLUSION: More research is being encouraged to increase understanding of the prevention and treatment of thrombotic complications of COVID-19.

scholarly journals Evaluation of blood plasma coagulation balance in patients with ischemic heart disease and percutaneous coronary angioplasty using total hematopoietic potential

2019 ◽  
Vol 23 (3) ◽  
pp. 397-400
Author(s):  
N.V. Storozhuk ◽  
V.P. Ivanov ◽  
B.H. Storozhuk ◽  
T.V. Dovgaliuk
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Blood Plasma ◽  
Statistical Processing ◽  
Risk Groups ◽  
Ischemic Heart ◽  
Percutaneous Coronary Angioplasty ◽  
Thrombotic Risk ◽  
Percutaneous Coronary ◽  
Study Results

Characterization of thrombotic risk in patients with ischemic heart disease and percutaneous coronary intervention (PCI) is important for preventive measures. The objective of the research is to develop an affordable way to determine the coagulation balance of blood plasma in patients with ischemic heart disease and PCI based on the clotting potential. Using the suggested coagulation index (CI), the indices of coagulation balance of 91 patients with coronary heart disease and PCI were determined, namely total hemostatic potential and fibrinolytic potential, their indices and index ratios, ie CIs. The results suggest that in 62.4% of the patients under investigation, a state of hypercoagulation is observed, which is always present in restenosis in the anamnesis and in patients with angina pectoris. Statistical processing of the study results was performed using methods of variational statistics using Student's t-test. The suggested CI allows the identification of high-thrombotic risk groups.

scholarly journals Frequency and nature of complications and hemostasis indicators in patients with ishemic artery disease, who had undergone percutaneous coronary angioplasty (based on the 18-month clinical observation)

2019 ◽  
Vol 23 (2) ◽  
pp. 248-250
Author(s):  
N. V. Storozhuk ◽  
T. V. Dovgalyuk ◽  
B. H. Storozhuk
Keyword(s):  
Protein C ◽  
Clinical Observation ◽  
Statistical Processing ◽  
Vascular Lesions ◽  
General Group ◽  
Percutaneous Coronary Angioplasty ◽  
Soluble Fibrin ◽  
Artery Disease ◽  
Late Restenosis ◽  
D Dimer

Determination of the nature, frequency and causes of restenosis/thrombosis in patients with ishemic artery disease and PKA in prospective observation is significant for the development of preventive measures depending on the clinical (comorbidity) and biochemical (state of the hemostasis system) characteristics of each patient. The goal is to study the frequency and nature of complications in patients with coronary artery disease and PKA and their hemostasis indicators. An 18-month follow-up (outpatient, inpatient and telephone mode) was performed for 91 patients, of whom 32 had a late restenosis/stent thrombosis in history (more than 6 months after stenting) and 59 – who did not have restenosis. All patients at the beginning of the study once determined the main indicators of hemostasis (soluble fibrin, D-dimer, fibrinogen and protein C). Patients followed by clinical observation did not receive anticoagulant treatment and received dual antiplatelet therapy. Statistical processing was performed using the methods of variation statistics and correlation analysis. The results of the study revealed that in the group of patients with restenosis and diabetes mellitus in the history, the risk of developing restenosis/thrombosis of the stent is twice as high as in the general group. Herewith, damage to other parts of the cardiovascular system is practically not excluded with the same frequency. The results of the study also revealed significant abnormalities in the system of hemostasis in patients with the presence of both restenosis/thrombosis and other vascular lesions. Thus, in patients with thrombotic complications, there is a sharp increase in the factor of prethrombosis – soluble fibrin, with a clear depression of the fibrinolytic linkage of hemostasis, as indicated by the low level of D-dimer. In addition, these violations of the sF/D-d ratio are associated with inhibition of the anticoagulant ability of hemostasis represented by the protein C. Consequently, the course of the disease in patients with IAD and PKA is closely related to the presence of comorbid states and hemostasis

scholarly journals Prevalence of thrombotic complications in children with SARS-CoV-2

2021 ◽  
pp. archdischild-2020-321351
Author(s):  
David Aguilera-Alonso ◽  
Sara Murias ◽  
Amelia Martínez-de-Azagra Garde ◽  
Antoni Soriano-Arandes, ◽  
Marta Pareja ◽  
...  
Keyword(s):  
Risk Factors ◽  
Adolescent Girls ◽  
Thrombotic Complication ◽  
Thrombotic Risk ◽  
Hospitalised Patients ◽  
Paediatric Cohort ◽  
National Cohort ◽  
Thrombotic Complications ◽  
Anticoagulant Prophylaxis ◽  
D Dimer

Knowledge of thrombosis in children with SARS-CoV-2 is scarce. In this multicentre national cohort of children with SARS-CoV-2 involving 49 hospitals, 4 patients out of 537 infected children developed a thrombotic complication (prevalence of 0.7% (95% CI: 0.2% to 1.9%) out of the global cohort and 1.1% (95% CI: 0.3% to 2.8%) out of the hospitalised patients). We describe their characteristics and review other published paediatric cases. Three out of the four patients were adolescent girls, and only two cases had significant thrombotic risk factors. In this paediatric cohort, D-dimer value was not specific enough to predict thrombotic complications. Adolescence and previous thrombotic risk factors may be considered when initiating anticoagulant prophylaxis on children with SARS-CoV-2 disease (COVID-19).

APC Resistance and other Haemostatic Variables during Pregnancy and Puerperium

1999 ◽  
Vol 81 (04) ◽  
pp. 527-531 ◽  
Author(s):  
U. Kjellberg ◽  
N.-E. Andersson ◽  
S. Rosén ◽  
L. Tengborn ◽  
M. Hellgren
Keyword(s):  
Factor Viii ◽  
Plasminogen Activator ◽  
Protein C ◽  
Activated Protein C ◽  
Plasminogen Activator Inhibitor ◽  
Protein S ◽  
Reference Level ◽  
Soluble Fibrin ◽  
Prothrombin Fragment ◽  
D Dimer

SummaryForty-eight healthy pregnant women were studied prospectively and longitudinally. Blood sampling was performed at 10-15, 23-25, 32-34 and 38-40 weeks of gestation, within one week and at eight weeks postpartum. Classic and modified activated protein C ratio decreased as pregnancy progressed. In the third trimester 92% of the ratios measured with the classic test were above the lower reference level whereas all modified test ratios were normal. Slight activation of blood coagulation was shown with increased levels of prothrombin fragment 1+2, soluble fibrin and D-dimer. Fibrinogen, factor VIII and plasminogen activator inhibitor type 1 and type 2 increased. Protein S and tissue plasminogen activator activity decreased. Protein C remained unchanged. No correlation was found between the decrease in classic APC ratio and changes in factor VIII, fibrinogen, protein S, prothrombin fragment 1+2 or soluble fibrin, nor between the increase in soluble fibrin and changes in prothrombin fragment 1+2, fibrinogen and D-dimer.

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