scholarly journals HINFI POLYMORPHISM OF THE H-FABP GENE OF DIFFERENT BREEDS OF PIGS IN THE BELGOROD REGION OF RUSSIA

2021 ◽  
Vol 11 (4) ◽  
pp. 971-976
Author(s):  
Eduard A. Snegin ◽  
◽  
Anton A. Sychev ◽  
Olesia Yu. Artemchuk ◽  
Anatolii S. Barkhatov ◽  
...  
Keyword(s):  
Fabp Gene

scholarly journals Up-regulation of the expression of the gene for liver fatty acid-binding protein by long-chain fatty acids

1996 ◽  
Vol 319 (2) ◽  
pp. 483-487 ◽  
Author(s):  
Claire MEUNIER-DURMORT ◽  
Hélène POIRIER ◽  
Isabelle NIOT ◽  
Claude FOREST ◽  
Philippe BESNARD
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Fold Increase ◽  
Long Chain Fatty Acids ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Fabp Gene ◽  
Long Chain

The role of fatty acids in the expression of the gene for liver fatty acid-binding protein (L-FABP) was investigated in the well-differentiated FAO rat hepatoma cell line. Cells were maintained in serum-free medium containing 40 µM BSA/320 µM oleate. Western blot analysis showed that oleate triggered an approx. 4-fold increase in the cytosolic L-FABP level in 16 h. Oleate specifically stimulated L-FABP mRNA in time-dependent and dose-dependent manners with a maximum 7-fold increase at 16 h in FAO cells. Preincubation of FAO cells with cycloheximide prevented the oleate-mediated induction of L-FABP mRNA, showing that protein synthesis was required for the action of fatty acids. Run-on transcription assays demonstrated that the control of L-FABP gene expression by oleate was, at least in part, transcriptional. Palmitic acid, oleic acid, linoleic acid, linolenic acid and arachidonic acid were similarly potent whereas octanoic acid was inefficient. This regulation was also found in normal hepatocytes. Therefore long-chain fatty acids are strong inducers of L-FABP gene expression. FAO cells constitute a useful tool for studying the underlying mechanism of fatty acid action.

scholarly journals Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs

2008 ◽  
Vol 60 (2) ◽  
pp. 408-413 ◽  
Author(s):  
F.C. Figueiredo ◽  
P.S. Lopes ◽  
A.P.G. Pinto ◽  
D.A.F. Paiva ◽  
P.T. Mendonça ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Genetic Groups ◽  
Pcr Products ◽  
Fabp Gene

The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.

scholarly journals Genome-Wide Analysis of the FABP Gene Family in Liver of Chicken (Gallus gallus): Identification,Dynamic Expression Profile, and RegulatoryMechanism

2019 ◽  
Vol 20 (23) ◽  
pp. 5948 ◽  
Author(s):  
Wang ◽  
Yue ◽  
Liu ◽  
Yang ◽  
Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gene Family ◽  
Expression Profile ◽  
Gene Structure ◽  
Regulatory Mechanism ◽  
Fatty Acid Binding ◽  
Pparγ Agonist ◽  
Ppar Agonists ◽  
Pparγ Agonists ◽  
Fabp Gene

The fatty acid-binding protein (FABP) gene family, which encodes a group of fatty acid-trafficking molecules that affect cellular functions, has been studied extensively in mammals. However, little is known about the gene structure, expression profile, and regulatory mechanism of the gene family in chickens. In the present study, bioinformatics-based methods were used to identify the family members and investigate their evolutionary history and features of gene structure. Real-time PCR combined with in vivo and in vitro experiments were used to examine the spatiotemporal expression pattern, and explore the regulatory mechanism of FABP genes. The results show that nine members of the FABP gene family, which branched into two clusters and shared a conserved FATTYACIDBP domain, exist in the genome of chickens. Of these, seven FABP genes, including FABP1, FABP3-7, and FABP10 were abundantly expressed in the liver of hens. The expression levels of FABP1, FABP3, and FABP10 were significantly increased, FABP5 and FABP7 were significantly decreased, and FABP4 and FABP6 remained unchanged in hens at the peak laying stage in comparison to those at the pre-laying stage. Transcription of FABP1 and FABP3 were activated by estrogen via estrogen receptor (ER) α, whilst FABP10 was activated by estrogen via ERβ. Meanwhile, the expression of FABP1 was regulated by peroxisome proliferator activated receptor (PPAR) isoforms, of which tested PPARα and PPARβ agonists significantly inhibited the expression of FABP1, while tested PPARγ agonists significantly increased the expression of FABP1, but downregulated it when the concentration of the PPARγ agonist reached 100 nM. The expression of FABP3 was upregulated via tested PPARβ and PPARγ agonists, and the expression of FABP7 was selectively promoted via PPARγ. The expression of FABP10 was activated by all of the three tested PPAR agonists, but the expression of FABP4-6 was not affected by any of the PPAR agonists. In conclusion, members of the FABP gene family in chickens shared similar functional domains, gene structures, and evolutionary histories with mammalian species, but exhibited varying expression profiles and regulatory mechanisms. The results provide a valuable resource for better understanding the biological functions of individual FABP genes in chickens.

Association of A‐FABP gene polymorphism and mRNA expression with intramuscular fat content (IMF) in Baicheng‐You chicken

2019 ◽  
Vol 103 (5) ◽  
pp. 1447-1452 ◽  
Author(s):  
Yong Wang ◽  
Wenqiang Liu ◽  
Chao Hang ◽  
Yiqiang Du ◽  
Ying Chen ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Mrna Expression ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Intramuscular Fat Content ◽  
Fabp Gene

scholarly journals Mapping enteroendocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract.

1990 ◽  
Vol 110 (5) ◽  
pp. 1791-1801 ◽  
Author(s):  
K A Roth ◽  
J M Hertz ◽  
J I Gordon
Keyword(s):  
Gastrointestinal Tract ◽  
Transgenic Mice ◽  
Fusion Gene ◽  
Enteroendocrine Cells ◽  
Regional Differentiation ◽  
Cell Populations ◽  
Intestinal Epithelial ◽  
Enteroendocrine Cell ◽  
Fatty Acid Binding ◽  
Fabp Gene

The gastrointestinal tract is lined with a monolayer of cells that undergo perpetual and rapid renewal. Four principal, terminally differentiated cell types populate the monolayer, enterocytes, goblet cells, Paneth cells, and enteroendocrine cells. This epithelium exhibits complex patterns of regional differentiation, both from crypt-to-villus and from duodenum-to-colon. The "liver" fatty acid binding protein (L-FABP) gene represents a useful model for analyzing the molecular basis for intestinal epithelial differentiation since it exhibits cell-specific, region-specific, as well as developmental stage specific expression. We have previously linked portions of the 5' nontranscribed domain of the rat L-FABP gene to the human growth hormone (hGH) gene and analyzed expression of the fusion gene in adult transgenic mice. High levels of hGH expression were noted in enterocytes as well as cells that histologically resembled enteroendocrine cells. In the present study, we have used immunocytochemical techniques to map the distribution of enteroendocrine cells in the normal adult mouse gut and to characterize those that synthesize L-FABP. In addition, L-FABP/hGH fusion genes were used to identify subsets of enteroendocrine cells based on their ability to support hGH synthesis in several different pedigrees of transgenic mice. The results reveal remarkable differences in transgene expression between, and within, enteroendocrine cell populations previously classified only on the basis of their neuroendocrine products. In some cases, these differences are related to the position occupied by cells along the duodenal-to-colonic and crypt-to-villus axes of the gut. Thus, transgenes appear to be sensitive tools for examining the cellular and regional differentiation of this class of intestinal epithelial cells.

Association of A-FABP gene polymorphism in intron 1 with meat quality traits in Junmu No. 1 white swine

Gene ◽  
2011 ◽  
Vol 487 (2) ◽  
pp. 170-173 ◽  
Author(s):  
Yan Gao ◽  
Yonghong Hong Zhang ◽  
Shumin Zhang ◽  
Fujuan Li ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Meat Quality ◽  
Quality Traits ◽  
Meat Quality Traits ◽  
Intron 1 ◽  
Fabp Gene

Short Communication: Analysis of intramuscular fat and fatty acids of different duck breeds and their association with SNPs of duck A-FABP gene

2011 ◽  
Vol 91 (4) ◽  
pp. 593-596 ◽  
Author(s):  
Jun He ◽  
Lizhi Lu ◽  
Yong Tian ◽  
Zhengrong Tao ◽  
Deqian Wang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Short Communication ◽  
Intramuscular Fat ◽  
Lipid Binding ◽  
Communication Analysis ◽  
Muscle Weight ◽  
Fatty Acid Binding ◽  
Organoleptic Characteristics ◽  
Fabp Gene ◽  
The Imf

He, J., Lu, L., Tian, Y., Tao, Z., Wang, D., Li, J., Li, G., Shen, J., Fu, Y. and Niu, D. 2011. Short Communication: Analysis of intramuscular fat and fatty acids of different duck breeds and their association with SNPs of duck A-FABP gene. Can. J. Anim. Sci. 91: 593–596. Intramuscular fat (IMF) is related to organoleptic characteristics of meat. Adipocyte fatty acid-binding protein (A-FABP) is one of the intracellular lipid-binding proteins involved in the transportation of fatty acids. The IMF contents of six duck breeds were measured, and the complete sequence and part of the 5' flanking region of duck A-FABP gene were obtained in this study. The IMF contents of different breeds were significantly different (P<0.05). Two SNPs were detected in the exon 3, one (HQ640428: g.2018A>G) was significantly associated with the contents of three fatty acids, total IMF and pectoral muscle weight. This work provides useful data for duck breeding.

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