scholarly journals In vitro comparative study of two Arundo donax L. ecotypes’ selenium tolerance

2014 ◽  
Vol 20 (3-4) ◽  
Author(s):  
É. Domokos-Szabolcsy ◽  
N. A. Alla ◽  
T. Alshaal ◽  
A. Sztrik ◽  
L. Márton ◽  
...  
Keyword(s):  
Survival Rate ◽  
Comparative Study ◽  
In Vitro Culture ◽  
Somatic Embryo ◽  
Arundo Donax ◽  
Sodium Selenate ◽  
Inhibiting Effect ◽  
Selenium Accumulation ◽  
Arundo Donax L

Selenium tolerance of two somatic embryo-derived Arundo donax L. ecotypes (Blossom, 20SZ) were compared in in vitro culture. Sodium-selenate (1 – 100 mg L-1) as known the most phytoavailable selenium form and the less studied red elemental nanoselenium (100 mg L-1) were applied as selenium treatments. Basis on the results Blossom ecotype seemed to be more sensitive to the sodium-selenate than 20SZ. Inhibiting effect of selenate was effectuated above 10 mg L-1 in case of Blossom, which was manifested in decreased survival rate and growing parameters. Contrast to this 20SZ could tolerate the selenate ≤ 20 mg L-1 without any toxic symptoms. Lower selenate tolerance of Blossom could be explained with higher selenium accumulation. Both of two ecotypes could also uptake and accumulate the red elemental nanoselenium however in much less extent compared to selenate.

Optimizing in vitro large scale production of giant reed (Arundo donax L.) by liquid medium culture

2014 ◽  
Vol 69 ◽  
pp. 21-27 ◽  
Author(s):  
Valeria Cavallaro ◽  
Cristina Patanè ◽  
Salvatore L. Cosentino ◽  
Isabella Di Silvestro ◽  
Venera Copani
Keyword(s):  
Liquid Medium ◽  
Large Scale ◽  
Arundo Donax ◽  
Giant Reed ◽  
Scale Production ◽  
Large Scale Production ◽  
Medium Culture ◽  
Arundo Donax L

DEVELOPMENT OF AN INTRODUCTION PROTOCOL FOR STRAWBERRY PLANTS INTO IN VITRO CULTURE

2020 ◽  
Vol 5 (86) ◽  
pp. 45-50
Author(s):  
O.V. Mazneva ◽  
◽  
L.V. Tashmatova ◽  
T.M. Khromova ◽  
V.V. Shakhov ◽  
...  
Keyword(s):  
Survival Rate ◽  
In Vitro Culture ◽  
Plant Material ◽  
Survival Rates ◽  
Strawberry Plant ◽  
Active Growth ◽  
High Viability ◽  
Average Survival ◽  
Average Survival Rate

The research was conducted in order to develop an effective protocol for introducing strawberry plants into in vitro culture. The objects of the research were the most popular varieties of strawberries of domestic and foreign selection: Tsaritsa, Bereginya, Florence, Frida, Kimberly, etc. Mercurial preparations mertiolate at a concentration of 0.01% and sulema at a concentration of 0.1% were used as sterilizing agents. The isolation of explants was performed in several periods: the beginning of the growth was in February, active growth was in June, the decline of growth was in August. The studies have shown that the maximum aseptic cultures were obtained when processing strawberry plant material with mercurycontaining sulema preparation in the concentration of 0.1%. At the first stage of micropropagation, explants had a high viability during all periods of the isolation, the average survival rate for varieties was 74.8-80.7%. A significant influence of the genotype (varietal characteristics) on the survival rates of explants was noted. The number of explants suitable for cloning did not depend on the overall level of regeneration. Stabilization of the crop during winter introduction was much faster than in other periods. Using the winter term of the isolation of strawberry explants allowed to increase the yield of explants capable of further cloning, accelerate the stabilization of the culture in vitro and reduce the time for obtaining micro-plants suitable for planting in non-sterile conditions. On average, 75.2% of explants capable of further cloning for the varieties were obtained. As a result of the research, the conditions and methods for obtaining the largest number of viable sterile strawberry explants were worked out, which will be included into the process of reproduction in vitro and further research.

Assessing Arundo donax L. in vitro-tolerance for phytoremediation purposes

Chemosphere ◽  
2020 ◽  
Vol 252 ◽  
pp. 126576 ◽  
Author(s):  
J. Cano-Ruiz ◽  
M. Ruiz Galea ◽  
M.C. Amorós ◽  
J. Alonso ◽  
P.V. Mauri ◽  
...  
Keyword(s):  
Arundo Donax ◽  
Arundo Donax L

Cryotolerance of in vitro-produced porcine blastocysts is improved when using glucose instead of pyruvate and lactate during the first 2 days of embryo culture

2013 ◽  
Vol 25 (5) ◽  
pp. 737 ◽  
Author(s):  
M. Castillo-Martín ◽  
M. Yeste ◽  
R. Morató ◽  
T. Mogas ◽  
S. Bonet
Keyword(s):  
Survival Rate ◽  
Treatment Group ◽  
In Vitro Culture ◽  
Embryo Development ◽  
Embryo Culture ◽  
Embryo Quality ◽  
Cleavage Rate ◽  
Apoptosis Index ◽  
Number Of Cells

The objective of the present study was to determine the effects of replacing glucose with pyruvate and lactate during the first 48 h of in vitro culture (IVC) in NCSU-23 medium on embryo development, embryo quality and survival of porcine blastocysts after vitrification. To this end, in vitro-produced (IVP) porcine oocytes were cultured with either glucose for 6 days (IVC-Glu) or pyruvate–lactate from Day 0 to Day 2 and then with glucose until Day 6 (IVC-PyrLac). Blastocysts were vitrified on Day 6 using the Cryotop device and, after warming, survival rate and the apoptosis index were evaluated after 24 h incubation in NCSU-23 medium. No significant differences were observed between IVC-Glu and IVC-PyrLac in terms of cleavage rate, blastocyst yield, total number of cells per blastocyst or the apoptosis index (1.82 ± 0.75% vs 3.18 ± 0.88%, respectively) of non-vitrified embryos. However, a significant increase was seen in hatching/hatched blastocysts in the IVC-PyrLac compared with IVC-Glu treatment group (12.71 ± 1.20% vs 3.54 ± 0.47%, respectively). Regardless of treatment, vitrification impaired the survival rate and the apoptosis index. When comparing both treatments after warming, the percentage of apoptotic cells was significantly higher for blastocysts in the IVC-PyrLac compared with IVC-Glu group (18.55 ± 3.49% vs 9.12 ± 2.17%, respectively). In conclusion, under the conditions of the present study, replacement of glucose with pyruvate–lactate during the first 48 h of culture resulted in a lower cryotolerance of IVP porcine embryos.

scholarly journals Giant reed (Arundo donax L.) from ornamental plant to dedicated bioenergy species: review of economic prospects of biomass production and utilization

2018 ◽  
Vol 24 (1-2) ◽  
Author(s):  
G. Antal
Keyword(s):  
Biomass Production ◽  
High Energy ◽  
Arundo Donax ◽  
Giant Reed ◽  
Stem Cuttings ◽  
Invasive Weed ◽  
Advantages And Disadvantages ◽  
Tropical Conditions ◽  
Arundo Donax L

Giant reed (Arundo donax L.) is a perennial, herbaceous grass, it has been spread all over the world from continent to tropical conditions by human activities. In continental climate, especially Hungary, it has been considered as ornamental species, due to its decorative appearance, striped variants’ colour of leaves, long growing season and low maintenance requirements. It does not produced viable seeds, so it can be propagated vegetative ways by rhizomes or stem cuttings and by in vitro biotechnology methods. Because of its growth habits and good adaptation capability, it has been considered invasive weed primarily in coastal regions in warmer climate areas. In the previous century, giant reed produced for paper/cellulose/viscose production, woodwind musical instruments, stakes for plants or fishing rods etc. Over the last few decades, it has been produced for bioenergy purposes (bioethanol, biogas, direct combustion) or utilize as chemical basic compounds or construction materials. It has been considered a dedicated promising biomass crops thanks to high biomass production, high energy balance of cultivation and adaptability of different kind of soils and conditions. The objective of the present paper is to overview the most significance literature data on giant reed production and utilization, compare to own experimental data and economic calculations and to determine some critical factors, advantages and disadvantages of giant reed production compare to other biomass species.

scholarly journals In Vitro Culture of Seaweed Kappaphycus alvarezii under Different Formulation of Growth Stimulating Substances and Culture Media

2019 ◽  
Vol 7 (1) ◽  
pp. 274
Author(s):  
Mega D. Dalero ◽  
Grevo S. Gerung ◽  
Edwin L.A. Ngangi ◽  
Lawrence J.L. Lumingas ◽  
Markus T. Lasut
Keyword(s):  
Abscisic Acid ◽  
Survival Rate ◽  
In Vitro Culture ◽  
Culture Media ◽  
Kappaphycus Alvarezii ◽  
Seed Stock ◽  
In Vitro Growth ◽  
Parent Plant ◽  
Lower Growth

This study aims at obtaining a sustainably superior seed stock following the characteristics of the parent plant, determining the best formulation of the growth stimulating substance. In general, cytokinin and auxin combination was used, but this study also added with the combination of cytokinin and giberelin and cytokinin and abscisic acid (AA).Parameters measured were bud length, number of buds, and survival rate. Bacterial Vibrio sp test was also done as a cause of the explant mortality. Results showed that the longest bud was recorded in treatment C (S+A 1:2.5) cultured in a jar, 1.343 mm long, 38% of survival, while the highest number of buds was found in treatment B (S+A 1 : 2) 8.86. The shortest bud was recorded in treatment J (S + AA 1:2.5) cultured in a jar, 0.093 mm long, 2.64 buds, 10% of survival, while the explant cultured in the bottle had a length of 0.051 mm long, 1.50 buds, 4% of survival. As conclusion, the best growth stimulating substance was found in the treatment C for the bud length and the survival rate, while the best number of bud was recorded in the treatment B. The best culture tank was topless bottle (aerated). In vitro culture could also use S + G formulation. The explant mortality was caused by Vibrio charchariae. The use of S + AA formulation had lower growth than that of control treatment.Keywords :in vitro, growth stimulating substance, culture media, Kappaphycus alvarezii, Vibrio charchariae ABSTRAKPenelitian ini bertujuan untuk memperoleh benih unggul secara berkelanjutan yang mengikuti karakteristik dari tanaman induk, menentukan formulasi terbaik dari substansi pertumbuhan merangsang. Secara umum, kombinasi sitokinin dan auksin digunakan, tetapi penelitian ini juga menambahkankombinasi sitokinin, giberelin, sitokinin dan asam absisat (AA). Parameter yang diukur adalah panjang tunas, jumlah tunas, dan tingkat kelangsungan hidup. Bakteri Vibrio Uji sp juga dilakukan sebagai penyebab kematian eksplan . Hasil penelitian menunjukkan bahwa tunas terpanjang terdapat pada perlakuan C (S + A 1: 2,5)  kultur dalam toples, 1,343 mm, 38% hidup, sementara jumlah tertinggi tunas ditemukan pada perlakuan B (S + A 1: 2) 8.86 . Jumlah tunas paling sedikit terdapat pada perlakuan J (S + AA 1: 2,5) yang dikultur dalam toples, 0,093 mm, 2,64 tunas, 10% hidup, sedangkan eksplan yang dikultur dalam botol memiliki panjang 0.051 mm, 1. 50 tunas , 4% bertahan hidup. Sebagai kesimpulan, pertumbuhan terbaik merangsang zat ditemukan dalam perlakuan C untuk panjang tunas dan tingkat kelangsungan hidup, sementara jumlah tunas terbanyak ditemukan pada perlakuan B. Penggunaan wadah budaya terbaik adalah topless yang diaerasi. Kultur in vitro juga dapat menggunakan formulasi S + G. Kematian eksplan disebabkan oleh Vibrio charchariae . Penggunaan formulasi S + AA memiliki pertumbuhan yang lebih rendah dari pada pengobatan kontrol .Kata kunci : in vitro, zat perangsang tumbuh, media kultur, Kappaphycus alvarezii, Vibrio charchariae

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