scholarly journals Efficacy of the Visual, Minicolumn, and Thin Layer Chromatography Methods to Test Farmers Stock Peanuts for Aflatoxin1

1986 ◽  
Vol 13 (2) ◽  
pp. 74-77 ◽  
Author(s):  
T. B. Whitaker ◽  
J. W. Dickens
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Computer Models ◽  
Visual Method ◽  
Aflatoxin Concentration ◽  
Layer Chromatography

Abstract This study estimated the efficacy of the visual A. flavus (VAF), minicolumn (MCL), and thin layer chromatography (TLC) methods to detect farmers stock peanuts which contain aflatoxin. Aflatoxin tests on grade samples from each of 2300 lots of farmers stock peanuts was used to estimate the distribution of farmers stock lots according to their aflatoxin concentration (lot distribution). This lot distribution (with an average aflatoxin concentration of 59.5 parts per billion) was incorporated into each of the 3 computer models that simulate the testing of farmers stock peanuts for aflatoxin when the VAF, MCL, and TLC methods are used. The number of lots accepted and the average aflatoxin concentration (AA) in the accepted lots was predicted. Results indicate that when a given percentage of the lots are accepted, lots accepted by the VAF method have less aflatoxin than those lots accepted by either the MCL or TLC methods. When the present visual method was used to test the above lot distribution, 75.8% of the lots tested were accepted and the AA in the accepted and rejected lots were 4.1 and 232.8 parts per billion, respectively.

PRODUCTION OF AFLATOXIN IN PRE-PACKAGED LUNCHEON MEAT AND CHEESE AT REFRIGERATOR TEMPERATURES1

1971 ◽  
Vol 34 (7) ◽  
pp. 349-351 ◽  
Author(s):  
L. S. Oldham ◽  
F. W. Oehme ◽  
D. C. Kelley
Keyword(s):  
Public Health ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aspergillus Flavus ◽  
Aflatoxin Production ◽  
Aflatoxin Concentration ◽  
Luncheon Meat ◽  
The Common ◽  
Meat Samples ◽  
Layer Chromatography

Information is lacking on the ability of the common mold contaminant, Aspergillus flavus, to grow and produce aflatoxin in perishable foods at normal refrigerator temperatures. Because of public health interests we investigated the possibility of certain perishable foods contaminated with the mold developing an aflatoxin concentration under conditions of refrigeration. Cheddar cheese and luncheon-meat samples were inoculated with A. flavus ATCC 15517 and were refrigerated for 12 days at 4.4 or 7.2 C or incubated at 25 C for 12 days, Uninoculated cheese and meat samples were handled in the same manner. All samples were quantitatively analyzed by thin-layer chromatography for presence of aflatoxin. All samples; except those inoculated and incubated at 25 C, were negative for aflatoxin production. This indicated the mold would, not produce detectable aflatoxin in the tested foods when kept at normal refrigeration temperatures for 12 days.

Performance of the Visual, Minicolumn and TLC Methods in Detecting Aflatoxin in 20 Contaminated Lots of Farmers Stock Peanuts

1984 ◽  
Vol 11 (2) ◽  
pp. 77-83 ◽  
Author(s):  
J. I. Davidson ◽  
J. W. Dickens ◽  
V. Chew ◽  
T. H. Sanders ◽  
C. E. Holaday ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Coefficient Of Variation ◽  
Visual Methods ◽  
Acceptance Level ◽  
Mean Values ◽  
Analytical Results ◽  
Aflatoxin Concentration ◽  
Layer Chromatography ◽  
Standard Grade

Abstract Standard grade samples (16) from each of 20 selected minilots were used to evaluate three methods for detecting minilots of farmers stock peanuts with unacceptable concentrations of aflatoxin. A visual, a minicolumn and a modified thin layer chromatography (TLC) method were used to compare analytical results, variation, and probability of acceptance for minilots having mean aflatoxin concentrations ranging from 8–255 ppb. Mean values obtained by each of the three methods increased linearly with mean aflatoxin concentrations of the minilots and variation for each method as determined by the variance and coefficient of variation (CV) was very large. The CV for all three methods decreased as aflatoxin concentration increased. Overall performances of the three methods were similar in accepting and rejecting these minilots on the basis of the 1.8 kg grade samples. The greatest difference in the three methods occurred at the zero acceptance level where the modified TLC, minicolumn and visual methods rejected 97, 98 and 88%, respectively, of the minilots with more than 60 ppb aflatoxin. At this acceptance level the TLC, minicolumn and visual methods also rejected 55, 50 and 30%, respectively, of the minilots with less than 30 ppb aflatoxin.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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