In vitro studies of amoxicillin on antibacterial activity for some fish pathogens.

Crude methanol extract of 9 Vietnamese plants were in vitro screened for their antibacterial activity against three common freshwater fish pathogens including Aeromonas hydrophila, Edwardsiella ictaluri, and Streptococcus agalactiae. Agar disc diffusion method was used to evaluate the antibacterial activity, then solvent extract was performed for the extracts which exhibited the strongest and a broad-spectrum antibacterial activity. Minimal inhibitory concentration (MIC) was conducted for effective plant extracts using broth dilution method. The results indicated that most of the plant extracts exhibited antibacterial propeties to at least one tested bacterium. Headache tree (Premna corymbosa), bushwillows (Combretum quadrangulare) and Celandine spider flower (Cleome chelidonii) showed a broad-spectrum antibacterial activity. The largest inhibitory zones of 35 mm and 21 mm were observed for the extract of Premna corymbosa against E. ictaluri and S. agalactiae, respectively. E. ictaluri was found to be the most susceptible for all of the extracts while A. hydrophila was the most resistant. The MIC of effective plant extracts against tested bacteria ranged between 0.39 mg/mL and 3.125 mg/mL. The result can be considered for further investigation of the development of an alternative therapy against bacterial infection in aquaculture.

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Preliminary in vitro screening of the antibacterial activity of leaf extracts from various Ficus species (Moraceae) against Yersinia ruckeri

Fisheries & Aquatic Life ◽

10.2478/aopf-2019-0002 ◽

2019 ◽

Vol 27 (1) ◽

pp. 15-26 ◽

Cited By ~ 1

Author(s):

Halyna Tkachenko ◽

Lyudmyla Buyun ◽

El¿bieta Terech-Majewska ◽

Vitaliy Honcharenko ◽

Andriy Prokopiv ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antibacterial Properties ◽

Yersinia Ruckeri ◽

Leaf Extracts ◽

Fish Pathogens ◽

Promising Alternative ◽

Ethanolic Extracts ◽

Activity Screening

Abstract Remarkable progress in the field of antibacterial herbal therapy has been made in recent decades in response to the development of drug-resistant pathogens in aquaculture. Studies have focused on the in vitro antimicrobial activity screening of ethanolic extracts of various plants belonging to the genus Ficus. The aim of the present study was to evaluate the antibacterial efficacy of ethanolic extracts of various Ficus species against Yersinia ruckeri. In vitro tests for antibacterial activity revealed that ethanolic leaf extracts of various Ficus species and their cultivars offer a promising alternative to antibiotics and chemotherapeutics for controlling the growth of Y. ruckeri. In our study, ethanolic extracts obtained from leaves of F. natalensis subsp. leprieurii and F. macrophylla proved effective against a bacterial strain at a dose of 400 pl standardized inoculum (108 CFU ml−1). It should be noted that Y. ruckeri demonstrated an intermediate susceptibility to more extracts derived from the leaves of Ficus species. Our investigation showed that among the various Ficus species, ethanolic leaf extracts of ten Ficus species against Y. ruckeri were the most effective. The effect of the leaf extracts that expressed the highest antimicrobial activity (F. macrophylla, F. natalensis subsp. leprieurii) against Y. ruckeri was comparable to that of gentamicin. Therefore, preliminary screening indicated that the ethanolic leaf extracts of some Ficus species with antibacterial properties can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, including Y. ruckeri.

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HYDROGEL FORMULATION FROM CALOTROPIS GIGANTEA PLANT EXTRACT AGAINST FOOT ULCER CAUSING BACTERIA IN DIABETES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i11.37147 ◽

2021 ◽

pp. 96-99

Author(s):

RAMACHANDRAN AM ◽

SENTHIL PRABHU S ◽

DEVAKUMAR J ◽

RENGARAMANUJAM J ◽

KARTHIK SUNDARAM S ◽

...

Keyword(s):

Antibacterial Activity ◽

Plant Extract ◽

Foot Ulcer ◽

Ethanolic Extract ◽

In Vitro Studies ◽

Inhibitory Zone ◽

Control Groups ◽

Calotropis Gigantea ◽

Hydrogel Formulation

Objective: The objective of present study was to develop hydrogel formulations loaded with Calotropis gigantea leaves extract. Methods: The prepared hydrogel formulations were compliance with their color, odor, homogeneity, pH, and spreadability. Results: As all the formulations were complies with the all parameters and it can be suggest as a Good antibacterial gel. It is evident that, ethanolic extract of Calotropis gigantea showed a maximum inhibitory zone against bacteria associated with foot ulcer. Conclusions: The In vitro studies showed a quantity dependent increase in antibacterial activity against foot ulcer causing bacteria, a contraction which is higher than that produced by the control groups. These contractions were statistically significant (p<0.05), during the study with leave extract against foot ulcer causing bacteria in diabetes.

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