scholarly journals ULTRASTRUCTURE AND HEPATOCYTE KARYOCYTOMETRY OF QUAILS

2019 ◽  
Vol 51 (3) ◽  
pp. 36-41
Author(s):  
G.Z. Bronnikova ◽  
◽  
E.N. Skovorodin ◽  
Keyword(s):  
Microscopic Examination ◽  
Morphological Characteristics ◽  
Electron Microscopic Examination ◽  
Cell Ultrastructure ◽  
The Body ◽  
Qualitative Description ◽  
Synthetic Activity ◽  
Electron Microscopic ◽  
Pharmacological Agents ◽  
The Impact

The paper studies the functional morphology of quail hepatocytes. To conduct the research there was an electron-microscopic examination of quail liver. The structure of the nucleus and nucleolus were quantitatively assessed using objective methods of karyocytometry. Quail hepatocytes are found to have high synthetic activity. Hepatocyte karyocytometry enables to reveal hidden morphological characteristics of the cell ultrastructure being hardly detected under normal qualitative description of electron-diffraction photos. Quail hepatocyte karyocytometry indicates a pronounced structural and functional heterogeneity of hepatocytes. Therefore, electron-microscopic examination using karyocytometric methods is a highly informative method for assessing the morphological and functional state of the body and it should be used in assessing the impact of pharmacological agents on the body.

Perfusion Fixation of Whole Fish for Electron Microscopy

1975 ◽  
Vol 32 (3) ◽  
pp. 416-422 ◽  
Author(s):  
D. E. Hinton
Keyword(s):  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Complete Removal ◽  
Cell Ultrastructure ◽  
Whole Body ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Liver And Kidney ◽  
Excellent Preservation ◽  
Perfusion Fixation

A method for obtaining uniform fixation of whole bodies of fish for electron microscopic study is described. The procedure employs vascular perfusion of aldehyde fixatives through the proximal aorta of anesthetized fish. Light microscopic examination of semi-thin (0.5–1 μ) sections of Epon embedded material, stained with toluidine blue, showed near to complete removal of blood cells from vessels of gill, bone, skin, skeletal muscle, heart, exocrine pancreas, mesentery, glomerulus, and brain. Electron microscopic examination of liver and kidney, fixed by whole body perfusion, showed excellent preservation of cell ultrastructure.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Artifact-free electron microscopic immunocytochemistry on rat brain tissue

1991 ◽  
Vol 49 ◽  
pp. 272-273
Author(s):  
Veronika Burmeister ◽  
N. Ludvig ◽  
P.C. Jobe
Keyword(s):  
Microscopic Examination ◽  
Free Electron ◽  
Electron Microscopic Examination ◽  
Ultrastructural Localization ◽  
Rabbit Serum ◽  
Electron Microscopic ◽  
Electron Microscopic Immunocytochemistry ◽  
Phosphate Buffered Saline ◽  
Rat Brain Tissue ◽  
Immune Rabbit

Electron microscopic immunocytochemistry provides an important tool to determine the ultrastructural distribution of various molecules in both normal and pathologic tissues. However, the specific immunostaining may be obscured by artifactual immunoreaction product, misleading the investigator. Previous observations show that shortening the incubation period with the primary antibody from the generally used 12-24 hours to 1 hour substantially reduces the artifactual immunostaining. We now extend this finding by the demonstration of artifact-free ultrastructural localization of the Ca2/calmodulindependent cyclic nucleotide phosphodiesterase (CaM-dependent PDE) immunoreactivity in brain.Anesthetized rats were perfused transcardially with phosphate-buffered saline followed by a fixative containing paraformaldehyde (4%) and glutaraldehyde (0.25%) in PBS. The brains were removed, and 40μm sections were cut with a vibratome. The sections were processed for immunocytochemistry as described by Ludvig et al. Both non-immune rabbit serum and specific CaM-dependent PDE antibodies were used. In both experiments incubations were at one hour and overnight. The immunostained sections were processed for electron microscopic examination.

Ultrastructural studies of Chlamydia psittaci 6BC variant strains. I. Ultrastructure of the surface layers of egg-passaged 6BC strain

1973 ◽  
Vol 19 (8) ◽  
pp. 887-894
Author(s):  
Linda Poffenroth ◽  
J. W. Costerton ◽  
Nonna Kordová ◽  
John C. Wilt
Keyword(s):  
Chick Embryo ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Chlamydia Psittaci ◽  
Gram Negative Bacteria ◽  
Surface Layers ◽  
Electron Microscopic ◽  
Gram Negative ◽  
Ultrastructural Studies ◽  
First Time

Electron microscopic examination of a semipurified Chlamydia psittaci 6BC strain attenuated in chick embryo yolk sac revealed for the first time two morphologically distinct small elementary bodies which differ both in the ultrastructure of their surface layers and in their buoyant densities in sucrose gradients. Also, the morphology of the surface layers of the larger reticulate forms in cell-free systems is described for the first time. Many points of difference between the surface envelopes and internal structure of chlamydial particles and those of Gram-negative bacteria are discussed.

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