scholarly journals Definitions of antibodies to the newcastle disease virus in the yolk of birds of artificial nesting box in conditions of the North-East of Ukraine

2021 ◽  
pp. 88-95
Author(s):  
E. O. Yarys ◽  
E. S. Kolesnik ◽  
D. V. Muzyka ◽  
A. B. Chaplygina
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Egg Yolk ◽  
Ficedula Albicollis ◽  
Artificial Nests ◽  
North East ◽  
The North ◽  
Phoenicurus Phoenicurus ◽  
Serological Studies

Introduction. In the conditions of the North-East of Ukraine, the circulation of the newcastle disease virus in hollow-nesting birds of artificial nests box was studied. Serological studies of the yolks of eggs collected from hollow birds in the North-East of Ukraine to the Newcastle disease virus were carried out. PDefinitions of antibodies to the newcastle disease virus in eggs of artificial nesting birds and determining their role as a reservoir of the causative agent of the disease in the North-East of Ukraine.Methods. The work was carried out in 2019 from April to December in the department for the study of poultry diseases at the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine", as well as at the Department of Zoology at the H. S. Skovoroda Kharkiv National Pedagogical University. Serological studies of the yolks of eggs taken from a hollow nesting box of birds. Antibodies in egg yolk extracts from birds of artificial box nests against the virus of newcastle disease, were determined in the reaction of delayed hemagglutination (HAGA).Result. Antibodies to the newcastle disease virus in titers 1:2 (3,3%) were detected in Phoenicurus phoenicurusFicedula albicollisErithacus rubeculaeggs and Phoenicurus phoenicurus, titers1:64 (23,3%) and 1:128 (26,6%) were found in the yolks of all studied species of artificial nesting birds. The highest antibody titers are 1:256 (26,6%) and 1:512 (6,6%) in the yolks of Ficedula albicollisand Phoenicurus phoenicuruseggs.Originality.Discovered of antibodies to the newcastle disease virus in the yolk of birds of artificial nesting box in conditions of the North-East of Ukraine.Conclusion. According to the results of serological studies of the eggs of birds of four species (Ficedula albicollis, Parus major, Erithacus rubeculaand Phoenicurus phoenicurus), which nest in northeastern Ukraine, it was found that antibodies to the Newcastle disease virus were found in 30 (66.6%).Key words:antibodies, Newcastle disease, hollow-nesting birds, egg yolk extracts, artificial nests box.

scholarly journals Heat Inactivation of Newcastle Disease Virus (NDV) in Egg Yolk and Its Survivability in Mayonnaise

2008 ◽  
Vol 25 (1) ◽  
pp. 32-35
Author(s):  
Nobuhiro SASHIHARA ◽  
Mineo HASEGAWA ◽  
Toshiro IZUCHI ◽  
Hiroshi ITO ◽  
Toshihiro ITO
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Egg Yolk ◽  
Heat Inactivation

scholarly journals Factors Affecting the Output Signal of the Electrochemical Immunosensor Based on Chicken Egg Yolk Antibody for Detecting Newcastle Disease Virus

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Tran Thi Luyen ◽  
Huynh Dang Chinh ◽  
Tran Quang Thinh ◽  
Mai Anh Tuan
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Incubation Time ◽  
Newcastle Disease ◽  
Output Signal ◽  
Egg Yolk ◽  
Electrochemical Immunosensor ◽  
Antibody Concentration ◽  
Chicken Egg ◽  
Chicken Egg Yolk

In this study, the electrochemical immunosensor for detecting Newcastle disease virus using the chicken egg yolk antibodies (IgY) was developed. The developed immunosensor was built into a three-electrode system consisting of two gold electrodes (a working electrode and a counter electrode) and a quasi-reference Ag/AgCl electrode. The IgY antibodies against Newcastle disease virus were immobilized on the working electrode (immunosensor) and the effect of the antibody concentration and virus incubation time on the output signal of the immunosensors was investigated. The investigation results show that when the antibody concentration was 60 µg/mL and the virus incubation time was 1 hour, the output signal of the immunosensors reached its maximum value (∆Ipeak = 0.1602). The detection limit of the sensors was 102 EID50/mL (EID50: 50% Empryo Infective Dose) at 25 oC. There was a good linear relationship between the ∆Ipeak and the logarithm of the virus Newcastle concentration in the range from 102 to 106 EID50/mL. The linear equation is ∆Ipeak = 0.0280logN – 0.00368 with the correlation coefficient of R2 = 0.9972.

scholarly journals Awareness and antibody detection of Newcastle disease virus in a neglected society in Nigeria

2019 ◽  
Vol 12 (1) ◽  
pp. 112-118 ◽  
Author(s):  
Oluwafemi Babatunde Daodu ◽  
Julius Olaniyi Aiyedun ◽  
Rafiu Adebisi Kadir ◽  
Hauwa Motunrayo Ambali ◽  
Oladapo Oyedeji Oludairo ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Egg Yolk ◽  
Antibody Detection ◽  
Cross Sectional ◽  
Hemagglutination Inhibition Test ◽  
Egg Yolks ◽  
The Government

Aim: This study aimed to assess the level of awareness of rural poultry farmers on vaccination and to detect Newcastle disease virus (NDV) antibody in local birds (LB) and eggs in Kwara State, Nigeria. Materials and Methods: Data on farmers' attitude, knowledge, practices, and experiences on ND mortality were obtained through an interview using a structured cross-sectional checklist. NDV antibodies were detected in sera and egg yolks of local chickens (LC) and guinea fowls (GF) using hemagglutination inhibition test. Results: A total of 83 interviewees, 287 sera and 121 egg yolk extracts, were examined. The study revealed that 98.8% (82/83) of the interviewee had never vaccinated their flock before. 90% of the interviewee had reported high mortality in birds within 1-6 months old, while the major clinical signs were cold (40.4%) and torticollis (30.8%). Evidences of LB exposure to wild-type NDV were confirmed by the detection of NDV antibodies in 20.8% and 0% of LC and GF, respectively. The mortality differences experienced in <1 and 1-6 months old LB could be explained by the presence of maternally-derived NDV antibody (49.6%) in egg yolk. Conclusion: The study showed that LB suffers from NDV as a result of LB keepers' ignorance and neglect by the government. This has limited local investment and subsequent contribution to gross domestic product. This study suggests that the key factors to the prevention of ND remain awareness creation about poultry vaccination, production of affordable vaccines, and availability/accessibility to veterinarian (or trained personnel).

scholarly journals Indirect Method for Prediction of Hemagglutination Inhibition Antibody Titers to Newcastle Disease Virus in Chickens by Titration of Antibodies in Egg Yolk

2003 ◽  
Vol 15 (2) ◽  
pp. 184-187 ◽  
Author(s):  
SangGeon Yeo ◽  
Eva Nagy ◽  
Peter J. Krell
Keyword(s):  
Linear Regression ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Proper Time ◽  
Egg Yolk ◽  
Inhibition Antibody ◽  
Antibody Titers ◽  
Feasible Alternative

Attempts were made to establish methods for indirect prediction of hemagglutination inhibition (HI) antibody titers to Newcastle disease virus (NDV) in sera of laying hens and day-old chicks by determining if these are correlated to HI titers in egg yolks. For this purpose, geometric means of HI antibody titers in sera from 60 hens, yolks from 60 matched eggs, and sera from 180 day-old chicks of an identical vaccination program were measured and plotted. There was a significant correlation between HI antibody titers in yolks (X) and hens (Y), with a linear regression of Y = 23.24 + 0.47X and a correlation coefficient of r = 0.65. The linear regression between HI antibody titers in yolks (X) and chicks (Y) was Y = 6.33 + 0.36X ( r = 0.58). Immunity to NDV in hens and their offspring can be maintained effectively, and the proper time for the vaccination or booster can be determined by reference to HI titers predicted from the linear regression in the present study. The approach of testing egg yolk for HI titers provides a feasible alternative to determining HI titers from blood samples and eliminates stress in birds during blood sampling.

scholarly journals Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Luyen Thi Tran ◽  
Thinh Quang Tran ◽  
Ha Phu Ho ◽  
Xuan Thi Chu ◽  
Tuan Anh Mai
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Direct Detection ◽  
High Sensitivity ◽  
Field Measurements ◽  
Egg Yolk ◽  
Extraction Process ◽  
Electrochemical Immunosensor ◽  
Label Free

In this study, a simple, label-free, electrochemical immunosensor system, including a three-electrode transducer and a microchamber, was designed, fabricated, and integrated with focus toward the detection of Newcastle disease virus (NDV). The chicken egg yolk antibodies (IgY) against NDV were used as the biological recognition element, replacing purified IgG antibodies that require a complex extraction process and time-consuming. The IgY against NDV was immobilized on the sensor surface using PrA/GA and SAM/NHS approaches. The immunosensor showed high sensitivity with NDV concentrations ranging from 106 to 102 EID50/mL with good specificity, repeatability, and small standard deviations. Compared to traditional methods, the immunosensor with advantages such as simple fabrication, quick response, direct detection, and possibility for miniaturization by integrating the immunosensor with the microchamber is potential for applications in contamination studies and field measurements.

Evaluation of the U.S. Department of Agriculture's Egg Pasteurization Processes on the Inactivation of High-Pathogenicity Avian Influenza Virus and Velogenic Newcastle Disease Virus in Processed Egg Products

2013 ◽  
Vol 76 (4) ◽  
pp. 640-645 ◽  
Author(s):  
REVIS A. CHMIELEWSKI ◽  
JOAN R. BECK ◽  
DAVID E. SWAYNE
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Thermal Inactivation ◽  
Egg Yolk ◽  
Log Reduction ◽  
Egg Products ◽  
Velogenic Newcastle Disease Virus ◽  
Lower Temperature

Globally, 230,662 metric tons of liquid egg products are marketed each year. The presence of highly pathogenic avian influenza (HPAI) or Newcastle disease in an exporting country can legitimately inhibit trade in eggs and processed egg products; development and validation of pasteurization parameters are essential for safe trade to continue. The HPAI virus (HPAIV) A/chicken/Pennsylvania/1370/1983 (H5N2) and velogenic Newcastle disease virus (vNDV) AMPV-1/chicken/California/S01212676/2002 were inoculated into five egg products and heat treated at various times and temperatures to determine thermal inactivation rates to effect a 5-log viral reduction. For HPAIV and vNDV, the pasteurization processes for fortified, sugared, plain, and salted egg yolk, and homogenized whole egg (HPAIV only) products resulted in &gt;5-log reductions in virus at the lower temperature–longer times of U.S. Department of Agriculture (USDA)–approved Salmonella pasteurization processes. In addition, a &gt;5-log reduction of HPAIV was also demonstrated for the five products at the higher temperatures–shorter times of USDA-approved pasteurization processes, whereas the vNDV virus was adequately inactivated in only fortified and plain egg yolk products. For the salted and sugared egg yolk products, an additional 0.65 and 1.6 min of treatment, respectively, at 63.3°C was necessary to inactivate 5 log of vNDV. Egg substitute with fat does not have standard USDA pasteurization criteria, but the D59-value was 0.75 min, adequate to inactivate 5 log of vNDV in &lt;4 min.

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