scholarly journals Anti-depressant Activity of Hydroalcoholic Extract of Asperula odorata L. in Mice

2021 ◽  
Vol 10 ◽  
pp. e2206
Author(s):  
Mohammad Amin Hatami Nemati ◽  
Kimia Vatani ◽  
Zahra Abbasy ◽  
Mahsa Hadipour Jahromy ◽  
Poorya Davoodi
Keyword(s):  
Tail Suspension Test ◽  
Behavioral Tests ◽  
Hydroalcoholic Extract ◽  
Treatment Groups ◽  
Elevated Plus Maze Test ◽  
Treatment Of Depression ◽  
Plus Maze ◽  
Swimming Test ◽  
The Relationship ◽  
Phenolic And Flavonoid

Background: The relationship between the treatment of depression and plant-derived substances (e.g., flavonoids, coumarin, and scopoletin) has been demonstrated through interference with the monoamine system. The present study was planned to evaluate the anti-depressant effects of Asperula odorata L. plant through behavioral tests in mice. Material and Methods: In this experimental study, 35 male Syrian mice weighing 30-40 g were examined in five groups (n=7) as follow: received oral distilled water gavage (control), 10 mg/kg of fluoxetine solution gavage (reference standard), 10, 5, and 2.5 mg/kg of A. odorata L. extract gavage (treatment groups). After one week, all behavioral tests, including tail suspension test (TST), forced swimming test (FST), open field test (OFT), elevated plus maze test (EPMT), and fractionation tests were performed each morning for 4-6 h within five days. Results: The hydroalcoholic extract of A. odorata contained phenolic and flavonoid substances (Shinoda test confirmed flavonoid family). Administration of extract (10 and 5 mg/kg doses) versus fluoxetine (10 mg/kg dose) reduced the immobility of animals in both FST and TST (P<0.05). At the OFT, the administered extract increased the number of central square entries of animals with higher mobility (P<0.05). At a 10 mg/kg dose, the active flavonoid ingredients increased the mice's incline to entre and spent more time within no wall parts of EPMT (P<0.05). Conclusion: Our study suggests that the hydroalcoholic extract of A. odorata L. could have significant anti-depressant activity. [GMJ.2021;10:e2206]

scholarly journals Dose-Dependent, Antidepressant, and Anxiolytic Effects of a Traditional Medicinal Plant for the Management of Behavioral Dysfunctions in Animal Models

Dose-Response ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 155932581989126 ◽  
Author(s):  
Hafiz Muhammad Asif ◽  
Abdul Hayee ◽  
Muhammad Rahil Aslam ◽  
Khalil Ahmad ◽  
Abdul Sattar Hashmi
Keyword(s):  
Open Field ◽  
Elevated Plus Maze ◽  
Tail Suspension Test ◽  
Antidepressant Effect ◽  
Tail Suspension ◽  
Hydroalcoholic Extract ◽  
Elevated Plus Maze Test ◽  
Plus Maze ◽  
Immobility Time ◽  
Dose Dependent

The present work was carried out to assess the Onosma bracteatum anxiolytic and antidepressant properties. Swiss albino mice (male) were fed orally with hydroalcoholic extract at different doses 50, 100, and 200 mg 1 hour prior to test with the standard diazepam and fluoxetine. Anxiolytic and antidepressant activities were evaluated by using open field, elevated plus maze, force swimming, and tail suspension test. Results of open field test showed an increase in number of line crossing as well as number of rearing in dosage-dependent design. Although results of elevated plus maze test evidently showed antianxiety effect of O bracteatum by increasing the time spent in open arms along with decreasing the time spent in closed arms in dosage-dependent way. For the evaluation of antidepressant effect, O bracteatum diminished the immobility time and expanded mobility time in forced swim model in dosage-dependent way. Likewise, O bracteatum expanded time span of mobility along with diminished immobility time in tail suspension method in dosage-dependent way. Outcome demonstrated that plant at the dose of 200 mg/kg body weight showed significant potential which was similar to that standard diazepam and fluoxetine. Hence, O bracteatum may be used as potent natural psychotherapeutic agent against the mental disorders.

scholarly journals Investigation of the Pharmacological Properties of Lepidagathis hyalina Nees through Experimental Approaches

Life ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 180
Author(s):  
Fowzul Islam Fahad ◽  
Niloy Barua ◽  
Md. Shafiqul Islam ◽  
Syed Al Jawad Sayem ◽  
Koushik Barua ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Elevated Plus Maze ◽  
Tail Suspension Test ◽  
Clot Lysis ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Swimming Test ◽  
Dose Dependent

Lepidagathis hyalina Nees is used locally in Ayurvedic medicine to treat coughs and cardiovascular diseases. This study explored its pharmacological potential through in vivo and in vitro approaches for the metabolites extracted (methanolic) from the stems of L. hyalina. A qualitative phytochemical analysis revealed the presence of numerous secondary metabolites. The methanol extract of L. hyalina stems (MELHS) showed a strong antioxidative activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power assays, and in the quantitative (phenolic and flavonoid) assay. Clot lysis and brine shrimp lethality bioassays were applied to investigate the thrombolytic and cytotoxic activities, respectively. MELHS exhibited an expressive percentage of clot lysis (33.98%) with a moderately toxic (115.11 μg/mL) effect. The in vivo anxiolytic activity was studied by an elevated plus maze test, whereas the antidepressant activity was examined by a tail suspension test and forced swimming test. During the anxiolytic evaluation, MELHS exhibited a significant dose-dependent reduction of anxiety, in which the 400 mg/kg dose of the extract showed 78.77 ± 4.42% time spent in the open arm in the elevated plus maze test. In addition, MELHS demonstrated dose-dependent and significant activities in the tail suspension test and forced swimming test, whereas the 400 mg/kg dose of the extract showed 87.67 ± 6.40% and 83.33 ± 6.39% inhibition of immobile time, respectively. Therefore, the current study suggests that L. hyalina could be a potential source of anti-oxidative, cytotoxic, thrombolytic, anxiolytic, and antidepressant agents. Further study is needed to determine the mechanism behind the bioactivities.

scholarly journals THE COMBINED EFFECT OF DEXAMETHASONE AND BACILLUS CALMETTE–GUÉRIN ON THE NEUROBEHAVIORAL ASPECT IN MALE MICE (MUS MUSCULUS)

2019 ◽  
pp. 195-201
Author(s):  
ABDELKRIM HAOULI ◽  
SAMIR DJEMLI ◽  
ABDELKRIM TAHRAOUI
Keyword(s):  
Forced Swimming Test ◽  
Elevated Plus Maze ◽  
Tail Suspension Test ◽  
Combined Effect ◽  
Bacillus Calmette Guerin ◽  
Behavioral Tests ◽  
Male Mice ◽  
Bacille Calmette Guerin ◽  
Plus Maze ◽  
Swimming Test

Objective: Our study aims to evaluate the combined effect of Bacille Calmette–Guérin (BCG) and dexamethasone (DEX) on male mice undergoing behavioral testing (forced swimming test, elevated plus maze, tail suspension test, and sucrose intake). An intraperitoneal injection of 0.2 ml/mg of BCG and 0.1 ml/mg of DEX body weight was administered after all behavioral tests were applied. Methods: This study is based on the distribution of 15 male mice in three groups: Controls, DEX, and DEX+BCG. These mice underwent behavioral tests. Results: The result after administration of DEX and BCG was found after 10 days. We observed an increase in locomotors activity with some degree of anxiety. Conclusion: This allowed us to conclude that the combination of DEX+BCG seems to play a depressinogenic effect that will develop to become antidepressive.

scholarly journals Effects of test experience, closed-arm wall color, and illumination level on behavior and plasma corticosterone response in an elevated plus maze in male C57BL/6J mice: a challenge against conventional interpretation of the test

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hirotaka Shoji ◽  
Tsuyoshi Miyakawa
Keyword(s):  
Elevated Plus Maze ◽  
Plasma Corticosterone ◽  
Illumination Level ◽  
Test Battery ◽  
Lower Percentage ◽  
Behavioral Tests ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Corticosterone Response

AbstractThe elevated plus maze test is a widely used test for assessing anxiety-like behavior and screening novel therapeutic agents in rodents. Previous studies have shown that a variety of internal factors and procedural variables can influence elevated plus maze behavior. Although some studies have suggested a link between behavior and plasma corticosterone levels, the relationships between them remain unclear. In this study, we investigated the effects of experience with a battery of behavioral tests, the wall color of the closed arms, and illumination level on the behavior and plasma corticosterone responses in the elevated plus maze in male C57BL/6J mice. Mice were either subjected to a series of behavioral tests, including assessments of general health and neurological function, a light/dark transition test, and an open field test, or left undisturbed until the start of the elevated plus maze test. The mice with and without test battery experience were allowed to freely explore the elevated plus maze. The other two independent groups of naïve mice were tested in mazes with closed arms with different wall colors (clear, transparent blue, white, and black) or different illumination levels (5, 100, and 800 lx). Immediately after the test, blood was collected to measure plasma corticosterone concentrations. Mice with test battery experience showed a lower percentage of open arm time and entries and, somewhat paradoxically, had lower plasma corticosterone levels than the mice with no test battery experience. Mice tested in the maze with closed arms with clear walls exhibited higher open arm exploration than mice tested in the maze with closed arms with black walls, while there were no significant differences in plasma corticosterone levels between the different wall color conditions. Illumination levels had no significant effects on any measure. Our results indicate that experience with other behavioral tests and different physical features of the maze affect elevated plus maze behaviors. Increased open arm time and entries are conventionally interpreted as decreased anxiety-like behavior, while other possible interpretations are considered: open arm exploration may reflect heightened anxiety and panic-like reaction to a novel situation under certain conditions. With the possibility of different interpretations, the present findings highlight the need to carefully consider the test conditions in designing experiments and drawing conclusions from the behavioral outcomes in the elevated plus maze test in C57BL/6J mice.

scholarly journals Fast-acting effects of l-tetrahydropalmatine on depression and anxiety in mice

2019 ◽  
pp. 01-12
Author(s):  
Rui Li ◽  
Wai-Kin Mat ◽  
Wing-Man Chan ◽  
T Yiu-Cheong Ho ◽  
Rigil K Yeung ◽  
...  
Keyword(s):  
Gabaa Receptor ◽  
Elevated Plus Maze ◽  
Tail Suspension Test ◽  
Tail Suspension ◽  
Elevated Plus Maze Test ◽  
Gabaa Receptor Antagonist ◽  
Plus Maze ◽  
Antidepressant Effects ◽  
Fast Acting ◽  
Suspension Test

The racemate dl-tetrahydropalmatine (dl-THP) is known for its analgesic and sedative effects, and has been shown by us to be a potential agent for the treatment of anxiety.Herein, to delineate the therapeutic potentials of its different isomeric forms, the behavioral effects of l-THP, dl-THP and d-THP were compared regarding their anxiolytic and antidepressant properties in mouse behavioral models using the elevated plus-maze test and tail suspension test respectively. The anxiolytic and antidepressant effects of both l-THP and dl-THP were evident in forty-five minutes following oral administration. Moreover, l-THP exhibited much greater anxiolytic potency in the elevated plus-maze (0.1-2.5 mg/kg) and antidepressant potency in the tail suspension test (0.5-5.0 mg/kg) than dl-THP, whereas d-THP was inactive in either of these tests. As well, l-THP enhanced sociability and preference for social novelty at 0.1-0.5 mg/kg in Crawley’s three-chamber behavioral tests, and inhibited the amphetamine-induced manic-like hyperactivity of amphetamine-sensitized mice at 0.05-0.2 mg/kg. These pharmacological actions of l-THP were unaccompanied by any significant locomotor or myorelaxant side-effects. Co-administration of flumazenil, a GABAA receptor antagonist, inhibited the anxiolytic and antidepressant effects of l-THP, even though the binding affinity of l-THP was higher for dopamine D2-like receptors than for GABAA receptors. On this basis, l-THP displayed potential as a fast-acting drug for the treatment of anxiety, depression and bipolar disorder. Keywords: l-THP; dl-THP; Anxiolysis; Antidepressant; GABAA receptor; Fast-acting

scholarly journals Electroacupuncture Promotes Proliferation of Amplifying Neural Progenitors and Preserves Quiescent Neural Progenitors from Apoptosis to Alleviate Depressive-Like and Anxiety-Like Behaviours

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Liu Yang ◽  
Na Yue ◽  
Xiaocang Zhu ◽  
Qiuqin Han ◽  
Bin Li ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Elevated Plus Maze ◽  
Neural Progenitors ◽  
Chronic Unpredictable Stress ◽  
Hippocampal Dentate Gyrus ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Swimming Test ◽  
Behavioural Tests

The present study was designed to investigate the effects of electroacupuncture (EA) on depressive-like and anxiety-like behaviours and neural progenitors in the hippocampal dentate gyrus (DG) in a chronic unpredictable stress (CUS) rat model of depression. After being exposed to a CUS procedure for 2 weeks, rats were subjected to EA treatment, which was performed on acupoints Du-20 (Bai-Hui) and GB-34 (Yang-Ling-Quan), once every other day for 15 consecutive days (including 8 treatments), with each treatment lasting for 30 min. The behavioural tests (i.e., forced swimming test, elevated plus-maze test, and open-field entries test) revealed that EA alleviated the depressive-like and anxiety-like behaviours of the stressed rats. Immunohistochemical results showed that proliferative cells (BrdU-positive) in the EA group were significantly larger in number compared with the Model group. Further, the results showed that EA significantly promoted the proliferation of amplifying neural progenitors (ANPs) and simultaneously inhibited the apoptosis of quiescent neural progenitors (QNPs). In a word, the mechanism underlying the antidepressant-like effects of EA is associated with enhancement of ANPs proliferation and preserving QNPs from apoptosis.

scholarly journals Screening of Anxiolytic and Antidepressant of Methanolic Leaves Extract of Syzygium Cumini in Mice

2020 ◽  
Vol 8 (2) ◽  
pp. 91-97
Author(s):  
Ahad Abdul Rehman ◽  
Azra Riaz ◽  
Muhammad Arif Asghar ◽  
Bushra Sikandar ◽  
Moona Baig
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Tail Suspension Test ◽  
Anxiety And Depression ◽  
High Dose ◽  
Syzygium Cumini ◽  
Immobility Duration ◽  
Plus Maze ◽  
Swimming Test

Background: Anxiety and depression are very common in clinical practice and reduce the overall quality of life. In recent years, various researchers have focused on natural products which are derived from medicinal plants. Studies suggested that diet rich in flavonoids, vitamins and antioxidants are the important components in reduction of anxiety and depression. Objective: Hence current investigation was aimed to assess the anxiolytic and antidepressant effects of Syzygium cumini in mice at 125, 250 and 500 mg/kg. Methods: These effects were mainly evaluated twice at 8th and 15th days by elevated plus maze, open field test, forced swimming test and tail suspension test. Results and Conclusion: In open field test S. cumini showed escalation rearing in numbers and its duration which indicates improved exploratory behavior and locomotor activity of the animals. In EPM, there was increase in entries numbers and time spent in open arm. Decrease in immobility duration observed at low dose while high dose increased immobility duration in FST. Hence outcomes of current study indicate that S. cumini have anxiolytic and anti-depressant effect.

Sign in / Sign up

Export Citation Format

Share Document