scholarly journals Investigation of the Dose-Enhancement Effects of Spherical and Rod-Shaped Gold Nanoparticles on the HeLa Cell Line

2020 ◽  
Vol 9 ◽  
pp. 1581
Author(s):  
Samad Amani ◽  
Alireza Mehdizadeh ◽  
Mohammad Mehdi Movahedi ◽  
Marzieh Keshavarz ◽  
Fereshteh Koosha
Keyword(s):  
Gold Nanoparticles ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cells ◽  
Current Treatment ◽  
X Rays ◽  
Hela Cell Line ◽  
X Ray ◽  
Cervical Cancer Cells ◽  
Gold Nanomaterials

Background: Cervical cancer cells are known as radioresistant cells. Current treatment methods have not improved the patients’ survival efficiently; thus, new therapeutic strategies are needed to enhance the efficacy of radiotherapy. Gold nanomaterials with different shapes and sizes have been explored as radiosensitizers. The present study compared the radiosensitizing effects of gold nanorods (AuNRs) with spherical gold nanoparticles (AuNPs) on the HeLa cell line irradiated with megavoltage X-rays. Materials and Methods: The cytotoxicity of AuNRs and AuNPs on HeLa cells in the presence and absence of 6-MV X-ray was investigated using the MTT assay. For this aim, HeLa cells were incubated with and AuNPs and AuNRs at various concentrations (5, 10, and 15 µg/mL) for 6 hours. Afterward, HeLa cells were irradiated with 6-MV X-ray at a single dose of 2 Gy. Results: The results showed that the addition of AuNRs and AuNPs could enhance the radiosensitivity of HeLa cells. Both AuNRs and AuNPs showed low toxicity on HeLa cells, while AuNRs were more toxic than AuNPs at the examined concentrations. Moreover, it was found that AuNRs could enhance the radiosensitivity of HeLa cells more than spherical-shaped AuNPs. Conclusion: This study revealed that the shape of nanoparticles is an effective factor when they are used as radiosensitizing agents during radiotherapy. [GMJ.2020;9:e1581]

scholarly journals A COMPARATIVE ANTICANCER STUDY OF BIOSYNTHESIZED NANOPARTICLES, DOXORUBICIN & NANO-DOX CONJUGATE AGAINST CERVICAL CANCER HELA CELL LINE

2020 ◽  
pp. 4-7
Author(s):  
M. R. Kamala Priya ◽  
Priya R. Iyer
Keyword(s):  
Cervical Cancer ◽  
Gold Nanoparticles ◽  
Cell Death ◽  
Cancer Therapy ◽  
Hela Cell ◽  
Cell Line ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Line ◽  
Vero Cell Line

Doxorubicin is the most common chemotherapy drug used in cancer therapy. Its usage is associated with various side-effects. In order to overcome the challenges in Doxorubicin administration, the present study has focussed on synthesizing a drug conjugate with biosynthesized gold nanoparticles and doxorubicin. The gold nanoparticles were biosynthesized using green extracts of medicinal plants with potential anticancer activities. The nanoparticle that possesses anticancer activity was conjugated with the drug for a combinatorial effect of the nanoparticles and the drug. The in vitro cytotoxicity was checked in Vero cell line through MTT assay. The in vitro anti proliferative effects were screened against cervical cancer in HeLa cell line. Fluorescence activated cell sorting analysis was carried out to detect the difference between live and dead cell populations. The preliminary confirmation was carried out in UV-VIS spectrophotometer. The morphological characterization was carried out by SEM and stability by Zeta potential. The IC50 of the nanocompounds demonstrated anti-proliferative activity against cervical cancer similar to the chemotherapeutic drug, Doxorubicin; additionally in a much lesser concentration of the drug. The in vitro cytotoxicity exhibited high viability of cells in Vero cell line with minimum viability of 80% in all the tested concentrations. There was a synergistic effect of the nanoparticles along with the drug; thereby an enhanced therapeutic efficiency was achieved. FACS analysis showed 36% of cell death in Dox treated HeLa cells whereas 96% of cell death in Nano-Dox treated HeLa cells. Nano-Dox conjugate has demonstrated high anticancer effects than drug alone Doxorubicin. Further biosynthesized nanomaterials based drug formulation can be developed as a potential strategy in cancer therapy.

Glyco-gold nanoparticle shapes enhance carbohydrate–protein interactions in mammalian cells

Nanoscale ◽  
2016 ◽  
Vol 8 (25) ◽  
pp. 12729-12735 ◽  
Author(s):  
Sivakoti Sangabathuni ◽  
Raghavendra Vasudeva Murthy ◽  
Preeti Madhukar Chaudhary ◽  
Manalee Surve ◽  
Anirban Banerjee ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Hela Cell ◽  
Cell Line ◽  
Gold Nanoparticle ◽  
Protein Interactions ◽  
Mammalian Cells ◽  
Hela Cell Line ◽  
Line P ◽  
Nanoparticle Shapes

Shape dependent uptake of glyco-gold nanoparticles (G-AuNPs) in a HeLa cell line.

scholarly journals In vitro Anticancer Activity Effect of Extracellular Metabolites of Some Bacterial Species on HeLa Cell Line.

2020 ◽  
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Line ◽  
Hela Cells ◽  
Bacterial Species ◽  
Hela Cell Line ◽  
Inhibitory Effects ◽  
Anticancer Effect ◽  
E Coli ◽  
Extracellular Metabolites

Background: Cancer is still one of the most serious problems that affect human health. Despite the intense efforts to develop treatments, effective agents are still not available. In some cases, conventional therapy could be harmful or fail because of emerging drug resistance. Therefore, the development of novel therapies against cancer is of utmost importance. Assessment of anticancer effects of bacterial metabolites on cancer cells may help in the process of finding new cheap, reliable, contentious and safe cancer therapy. Objective: To determine the anticancer effect of the extracellular metabolites of eight bacterial species on HeLa cell line. Methodology: Extracellular metabolites were prepared by isolating and culturing eight bacterial species (Escherichia coli, Staphylococcus aureus, Micrococcus, Pseudomonas aeruginosa, Lactic acid bacteria, Klebsiella, Proteus and E. coli with its phage) in liquid media. Tubes were incubated overnight and centrifuged. Supernatant was filtered and concentrated using Infra-Red concentrator. Different concentrations were prepared and their anticancer effect were evaluated using MTT cell proliferation assay. Results: Results showed variation among the eight bacteria concerning proliferation inhibition against HeLa cells in a time and concentration dependent manner. Pseudomonas and E. coli with its phage revealed considerable anticancer activity with 63% and 86% inhibitory effects (both at 1000 µg\ml) and IC50 of 301 and 1395 µg/dl at 24h respectively. While Proteus and Micrococcus showed low inhibitory effects and S. aureus enhanced the proliferation of HeLa cells at low concentrations. Conclusion: Among the tested bacteria, Pseudomonas and E. coli and its phage gave the best anticancer inhibitory effects against HeLa cells. Further studies using purified components of effective bacteria are recommended.

scholarly journals Cytotoxic activity of Pinus cembra L. needle extract: A preliminary study on HeLa cell line

2021 ◽  
Vol 57 (2) ◽  
pp. 93-98
Author(s):  
Cristina LUNGU ◽  
◽  
Cosmin-Teodor MIHAI ◽  
Gabriela VOCHITA ◽  
Daniela GHERGHEL ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cells ◽  
Pine Needles ◽  
Hela Cell Line ◽  
Pine Needle ◽  
Cytotoxic Effects ◽  
Antitumor Potential ◽  
Cembran Pine

The aim of this study was to investigate the cytotoxic effects of a hydromethanolic extract obtained from cembran pine needles in HeLa cell line. In this respect, the effects of needle extract on protein synthesis, viability, proliferation and cell cycle in HeLa cells were evaluated after 48 h treatment. Cembran pine needle extract dose-dependently decreased protein synthesis in HeLa cells causing 44.26% reduction in protein synthesis at 100µg/ml. At 25, 50 and 100µg/ml, it increased cell death in comparison with the control (20.99%, 21.49% and 23.63%, respectively vs. 9.83%). In addition, at 100µg/ml, cembran pine needle extract showed a remarkable antiproliferative effect whereas at 25 and 50µg/ml, it induced sub-G1 phase cells accumulation (11.68 ± 0.81% and 14.69 ± 0.56%, respectively in comparison with control, 6.03 ± 0.55%), an indicator of proapoptotic effects. Taken together, these results indicate that cembran pine needles are a source of compounds with antitumor potential which needs to be further investigated and exploited.

scholarly journals Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells.

1993 ◽  
Vol 120 (1) ◽  
pp. 5-13 ◽  
Author(s):  
T Nilsson ◽  
M Pypaert ◽  
M H Hoe ◽  
P Slusarewicz ◽  
E G Berger ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cells ◽  
Hela Cell Line ◽  
Frozen Sections ◽  
Specific Antibodies ◽  
Trans Golgi Network ◽  
Endogenous Protein ◽  
Golgi Network

Thin, frozen sections of a HeLa cell line were double labeled with specific antibodies to localize the trans-Golgi enzyme, beta 1,4 galactosyltransferase (GalT) and the medial enzyme, N-acetylglucosaminyltransferase I (NAGT I). The latter was detected by generating a HeLa cell line stably expressing a myc-tagged version of the endogenous protein. GalT was found in the trans-cisterna and trans-Golgi network but, contrary to expectation, NAGT I was found both in the medial- and trans-cisternae, overlapping the distribution of GalT. About one third of the NAGT I and half of the GalT were found in the shared, trans-cisterna. These data show that the differences between cisternae are determined not by different sets of enzymes but by different mixtures.

scholarly journals Comparison of Anticancer Effects of Hydroalcoholic Extracts of Camellia sinensis and Lepidium sativum L on HeLa Cell Line

2020 ◽  
Vol 13 (11) ◽  
Author(s):  
Somayeh Jahani ◽  
Zahra Heidari ◽  
Mehdi Azami ◽  
Bita Moudi
Keyword(s):  
Hela Cell ◽  
Growth Inhibition ◽  
Cell Line ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Hela Cells ◽  
Green Tea Extract ◽  
Lepidium Sativum ◽  
Hela Cell Line ◽  
Tea Extract

Background: The antioxidative activity of green tea and garden cress extract is of interest in cancer. Objectives: The current study aimed at evaluating the effect of hydroalcoholic extracts of Lepidium sativum (cress) and Camellia sinensis (green tea) on the culture medium of the HeLa cell line. Methods: Dulbecco’s Modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) was used to culture HeLa cells, which was exposed to the different concentrations of green tea and cress extracts at 24 hours and 48 hours. Cell viability and apoptotic cells were quantified by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT assay and propidium iodide, respectively. Results: The highest percentage of growth inhibition (85%) was observed at 100 μg/mL of the green tea extract after 48-hour treatment. The percentage of growth inhibition at 24 h after treatment was 83% for green tea (P > 0.05). The high growth inhibition percentage of HeLa cells at 100 μg/mL of cress extract at 24 hours and 48 hours (49.8%) after treatment was 27.92% and 49.8%, respectively (P > 0.05). Additionally, the cell apoptosis assay indicated that green tea and cress extracts had toxic effects on the HeLa cells. This effect was highest at the concentration of 100 μg/mL and more evident in green tea. Conclusions: It can be concluded that green tea extract compared with cress had a more cytotoxic effect against cervical cancer.

scholarly journals Anticancer potentials of leaf, stem, and root extracts of Achyranthes aspera L.

2020 ◽  
Vol 12 (3) ◽  
pp. 546-555
Author(s):  
Nafisehsadat OMIDIANI ◽  
Kailas D. DATKHILE ◽  
Rajkumar B. BARMUKH
Keyword(s):  
Hela Cell ◽  
Ethyl Acetate ◽  
Cell Line ◽  
Hela Cells ◽  
Root Biomass ◽  
Acetone Extract ◽  
Hela Cell Line ◽  
Root Extracts ◽  
Achyranthes Aspera ◽  
Therapeutic Uses

Achyranthes aspera L. (Amaranthaceae), an herbaceous roadside weed in various parts of India, has several therapeutic uses, including the treatment against cancer. This investigation was undertaken to identify the bioactive compounds conferring cytotoxicity to the extracts from its biomass. The powdered leaf, stem, and root biomass was extracted separately in ethyl acetate, acetone, ethanol, and methanol. Each extract was tested against the HeLa cell line for the cytotoxicity, but the root-acetone extract was the most cytotoxic. This extract revealed eleven bands. The solution obtained from the ninth band (Rf = 0.87±0.06) exhibited more than 90% inhibition of HeLa cells. The LCMS analysis of this solution showed the presence of 37 compounds, out of which few compounds had been reported from different plants to possess cytotoxicity in various systems.

scholarly journals Cell death induced by photodynamic therapy with the conjugate of gold nanoparticles-PpIX in HeLa cell line

2019 ◽  
Author(s):  
Alfonso Antonio Sequeda Juárez ◽  
Elizabeth Maldonado Alvarado ◽  
Eva Ramón Gallegos
Keyword(s):  
Gold Nanoparticles ◽  
Cell Death ◽  
Photodynamic Therapy ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cell Line

scholarly journals SACCHAROMYCES CEREVISIAE-INDUCED APOPTOSIS OF MONOLAYER CERVICAL CANCER CELLS

2017 ◽  
Vol 10 (8) ◽  
pp. 63 ◽  
Author(s):  
Teena Rajan ◽  
Benluvankar V ◽  
Vincent S
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Hela Cell ◽  
Cell Line ◽  
Hela Cells ◽  
Prolonged Exposure ◽  
Chromatin Condensation ◽  
Yeast Cells ◽  
Apoptotic Cells ◽  
Hela Cell Line ◽  
Induction Of Apoptosis

  Objective: The present study was undertaken to examine the effect of phagocytosis of killed yeast on the induction of apoptosis in monolayer of HeLa cells.Methods: HeLa cell line was incubated with different doses (1000-7.8 μg/ml) of heat-killed Saccharomyces cerevisiae for 24, 48, and 72 hrs. The cytotoxicity against HeLa cell line during different exposure hours was screened by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-tetrazolium bromide assay. Induction of apoptosis was further confirmed by morphological and biochemical examination. Antiproliferative effect of yeast was examined under inverted microscope. Cell morphological changes were analyzed by fluorescent staining with propidium iodide.Results: The results showed that yeast induces cytotoxicity against HeLa cells in concentrations and during prolonged exposure periods. The viability of HeLa cells decreased from 85% to 45% during 72 hrs of treatment with 1000 μg/ml of yeast cells. The inhibitory concentration 50% of heat-killed yeast required to induce 50% inhibition of HeLa cells was 62.5 μg/ml. Apoptotic cells showed signs such as cell enlargement, membrane blebbing, and chromatin condensation. Furthermore, cell cycle analysis showed that S. cerevisiae treated HeLa cells and showed a typical apoptosis pattern of DNA content that reflected sub-G0 phase (corresponding to apoptotic cells).Conclusion: Results from the present work show that the heat-killed yeast has anticancer activity and it includes apoptosis of HeLa cells in vitro.

Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides

1995 ◽  
Vol 108 (4) ◽  
pp. 1617-1627 ◽  
Author(s):  
C. Rabouille ◽  
N. Hui ◽  
F. Hunte ◽  
R. Kieckbusch ◽  
E.G. Berger ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Hela Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Hela Cells ◽  
Stable Expression ◽  
Hela Cell Line ◽  
Specific Antibodies ◽  
Hela Cell Lines ◽  
Golgi Network

The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possible by stable expression of epitope-tagged forms of these proteins or forms from species to which specific antibodies were available. NAGT I and Mann II had the same distribution occupying the medial and trans cisternae of the stack. GalT and SialylT also had the same distribution but they occupied the trans cisterna and the trans-Golgi network (TGN). These results generalise our earlier observations on the overlapping distribution of Golgi enzymes and show that each of the trans compartments of the Golgi apparatus in HeLa cells contains unique mixtures of those Golgi enzymes involved in the construction of complex, N-linked oligosaccharides.

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