scholarly journals Characterization of Staphylococcus aureus isolates in milk and the milking environment from small-scale dairy farms of São Paulo, Brazil, using pulsed-field gel electrophoresis

2012 ◽  
Vol 95 (12) ◽  
pp. 7377-7383 ◽  
Author(s):  
S.H.I. Lee ◽  
C.H. Camargo ◽  
J.L. Gonçalves ◽  
A.G. Cruz ◽  
B.T. Sartori ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Dairy Farms ◽  
Sao Paulo ◽  
Pulsed Field Gel Electrophoresis ◽  
Small Scale ◽  
São Paulo ◽  
Pulsed Field ◽  
Small Scale Dairy

Characterization of Listeria monocytogenes Isolates from 50 Small-Scale Austrian Cheese Factories

2006 ◽  
Vol 69 (6) ◽  
pp. 1297-1303 ◽  
Author(s):  
M. WAGNER ◽  
F. ELISKASES-LECHNER ◽  
P. RIECK ◽  
I. HEIN ◽  
F. ALLERBERGER
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Wide Distribution ◽  
Pulsed Field Gel Electrophoresis ◽  
Annual Production ◽  
Personal Hygiene ◽  
Small Scale ◽  
Pulsed Field ◽  
High Prevalence

One hundred eighty-one small-scale cheese factories (annual production <100,000 kg) were tested for the presence of Listeria monocytogenes in cheese and smear samples from 1997 to 2000. In total, 2615 samples were drawn. Fifty (27.6%) of 181 enterprises yielded L. monocytogenes. From 14 of the cheese-making facilities, we obtained more than four L. monocytogenes isolates. A total of 182 mostly cheese- and smear-borne L. monocytogenes strains were characterized by serotyping and pulsed-field gel electrophoresis. In 12 of 14 cheese factories, over half of the L. monocytogenes isolates were genetically indistinguishable by pulsetype. On average, genetically indistinguishable isolates were recovered for 11.9 months. Regarding serotypes, 27.3% of the isolates were of serovar 4b. Inadequate personal hygiene could explain the high prevalence of serovar 4b isolates in small-scale cheesemaking facilities. Forty-two percent of the serovar 4b isolates recovered from epidemiologically unlinked facilities (in comparison to 40 and 29% of the 1/2a and 1/2b isolates, respectively) were genetically indistinguishable from at least one other isolate. Indistinguishable serovar 1/2a and 1/2b isolates belonged to five and six different pulsetypes, respectively, whereas serovar 4b isolates belonged to only two pulsetypes. This finding suggested a wide distribution of genetically homologous serovar 4b isolates among the facilities tested in our study.

scholarly journals Characterization of Staphylococcus aureus Coagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreaks (1980–1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis

2000 ◽  
Vol 38 (10) ◽  
pp. 3746-3749 ◽  
Author(s):  
Akira Shimizu ◽  
Manabu Fujita ◽  
Hideo Igarashi ◽  
Michihiro Takagi ◽  
Naoko Nagase ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Food Poisoning ◽  
Pulsed Field Gel Electrophoresis ◽  
Chromosomal Dna ◽  
Time Intervals ◽  
Pulsed Field ◽  
Pfge Typing

Staphylococcus aureus coagulase type VII strains have been the strains most frequently isolated from staphylococcal food poisoning outbreaks in Tokyo, Japan. We applied pulsed-field gel electrophoresis (PFGE) of chromosomal DNA digested withSmaI to characterize 129 coagulase type VII strains. These were isolated from 129 cases occurring in outbreaks in 35 districts during a 16-year period (1980–1995). The 129 outbreak strains were classified into three types, designated A (n = 115), B (n = 10), and C (n = 4). Types A and C were further divided into 33 (A1 to A33) and 4 (C1 to C4) subtypes, respectively. Strains of the same subtypes were isolated from food poisoning cases in the same districts at time intervals of 1 or 2 to 5 years. PFGE typing appears to be a useful method for subdividing strains of S. aureus coagulase type VII. A combination of coagulase typing and PFGE typing would provide more detailed information than the former method alone in epidemiologic investigations of staphylococcal food poisoning.

Sign in / Sign up

Export Citation Format

Share Document