scholarly journals Bioavailability of aflatoxin B1 and ochratoxin A, but not fumonisin B1 or deoxynivalenol, is increased in starch-induced low ruminal pH in nonlactating dairy cows

2016 ◽  
Vol 99 (12) ◽  
pp. 9759-9767 ◽  
Author(s):  
D. Pantaya ◽  
D.P. Morgavi ◽  
M. Silberberg ◽  
F. Chaucheyras-Durand ◽  
C. Martin ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Ruminal Ph

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

Dietary exposure and health risk characterization of aflatoxin B1, ochratoxin A, fumonisin B1, and zearalenone in food from different provinces in Northern Vietnam

Food Control ◽  
2020 ◽  
Vol 112 ◽  
pp. 107108 ◽  
Author(s):  
Tuan Huu Do ◽  
Son Cao Tran ◽  
Chi Dinh Le ◽  
Ha-Binh Thi Nguyen ◽  
Phuong-Thao Thi Le ◽  
...  
Keyword(s):  
Health Risk ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Dietary Exposure ◽  
Risk Characterization ◽  
Northern Vietnam

scholarly journals Co-occurrence of aflatoxin B1, fumonisin B1, ochratoxin A and zearalenone in cereals and peanuts from Côte d’Ivoire

2006 ◽  
Vol 23 (10) ◽  
pp. 1000-1007 ◽  
Author(s):  
Béatrice Sangare-Tigori ◽  
Serge Moukha ◽  
H. James Kouadio ◽  
Anne-Marie Betbeder ◽  
Djédjé Sébastien Dano ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Cote D'ivoire ◽  
Aflatoxin B1 ◽  
Côte D’Ivoire ◽  
Fumonisin B1 ◽  
Cote D’Ivoire ◽  
Côte D'ivoire

scholarly journals Risk of exposure to aflatoxin B1, ochratoxin A, and fumonisin B1 from spices used routinely in Lebanese cooking

2021 ◽  
Vol 147 ◽  
pp. 111895
Author(s):  
Manar Al Ayoubi ◽  
Michele Solfrizzo ◽  
Lucia Gambacorta ◽  
Ian Watson ◽  
Nada El Darra
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Risk Of Exposure

scholarly journals Fluorescence Enhancement on Silver-Plated Plasma Micro-Nanostructured 3D Polymeric Microarray Substrates for Multiplex Mycotoxin Detection

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 392
Author(s):  
Georgios Koukouvinos ◽  
Chrysoula-Evangelia Karachaliou ◽  
Anastasia Kanioura ◽  
Katerina Tsougeni ◽  
Evangelia Livaniou ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescence Intensity ◽  
Aflatoxin B1 ◽  
Oxygen Plasma ◽  
Fluorescence Enhancement ◽  
Fumonisin B1 ◽  
Silver Deposition ◽  
Standard Glass ◽  
Glass Slides ◽  
Coefficients Of Variation

Oxygen plasma micro-nanostructured poly(methyl methacrylate) (PMMA) slides were modified through silver microparticle deposition to create microarray substrates that enhance the emitted fluorescence intensity. Silver deposition relied on a commercially available reagent and was completed in two 30-min incubation cycles of the substrate with the reagent. The fluorescence enhancement achieved using these substrates over flat PMMA slides was determined through the development of a microarray for the multiplexed detection of four mycotoxins, aflatoxin B1, ochratoxin A, fumonisin B1, and deoxynivalenol. It was shown that the implementation of silver-plated oxygen plasma micro-nanotextured PMMA substrates increased the signals obtained for aflatoxin B1 and ochratoxin A by approximately 2.8 times, 5.6 times for deoxynivalenol, and 16-times for fumonisin B1, compared to flat PMMA substrates. Most notably, this signal increase was not accompanied by a significant increase in the non-specific signal. In addition, the spot repeatability both across a single slide as well as between different slides was high, with coefficients of variation lower than 12%. The slides were also stable for at least three months, thus offering a microarray substrate with improved properties compared to standard glass slides, regarding both the absolute spot fluorescence intensity and between spots repeatability.

scholarly journals Effect of mycotoxin deactivator product supplementation on dairy cows

2012 ◽  
Vol 52 (9) ◽  
pp. 832 ◽  
Author(s):  
K. Kiyothong ◽  
P. Rowlinson ◽  
M. Wanapat ◽  
S. Khampa
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Feed Intake ◽  
Block Design ◽  
Fumonisin B1 ◽  
Milk Composition ◽  
Ammonia Nitrogen ◽  
Negative Effects ◽  
Ruminal Ph ◽  
Dietary Treatments

A total mixed ration (TMR) containing a blend of feedstuffs naturally contaminated with harmful mycotoxins was fed for 84 days to 24 primiparous and multiparous Holstein–Friesian × local dairy cows in a randomised complete block design. The dietary treatments consisted of a contaminated TMR diet plus various levels of the mycotoxin deactivator product (MDP) (0, 15, 30 or 45 g/head.day). Deoxynivalenol (DON), fumonisin B1 (FB1), zearalenone (ZON) and ochratoxin A (OTA) were found in the TMR at levels up to 720, 701, 541 and 501 μg/kg, whereas aflatoxin B1 (AfB1) and T-2 toxin (T-2) were found in the TMR at levels of 38 and 270 μg/kg, respectively. Rumen microbial ecology, ruminal volatile fatty acid (VFA) concentrations, ruminal microorganism populations, feed intake, total tract digestibility, milk yield, milk composition and serum immunoglobulin (Ig) concentrations were measured. The results revealed that the ruminal pH, ruminal ammonia nitrogen (NH3-N) concentration, total ruminal VFA concentrations and ruminal bacterial counts were significantly (P < 0.05) higher in supplemented than in non-supplemented cows. Ruminal protozoal counts were significantly (P < 0.05) lower in supplemented than in non-supplemented cows. DM intake, and digestibility of crude protein (CP) and neutral detergent fibre (NDF) were significantly (P < 0.05) higher in supplemented than in non-supplemented cows. Serum IgA concentrations were significantly (P < 0.05) higher in supplemented than in non-supplemented cows. Milk yield and milk protein were significantly (P < 0.05) higher in supplemented than in non-supplemented cows. On the basis of this experiment, it can be concluded that milk production and feed intake can be increased with the addition of MDP to cow diet in the presence of mycotoxins. These increases were accompanied by decreases in the negative effects of mycotoxins on rumen and immune function.

scholarly journals Analytical Survey on Aflatoxin B1, Ochratoxin A, Fumonisin B1 and Fumonisin B2 of Cambodian Rice

2012 ◽  
Vol 2 (9) ◽  
Author(s):  
Dalin Ly ◽  
Sarom Men ◽  
Vibol San ◽  
Noel Durand ◽  
Jean-Claude Manez ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Fumonisin B2

scholarly journals The Investigation of Mycotoxins and Enterobacteriaceae of Cereal-Based Baby Foods Marketed in Turkey

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3040
Author(s):  
Buket Er Er Demirhan ◽  
Burak Demirhan
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Fumonisin B1 ◽  
Culture Method ◽  
Aflatoxin M1 ◽  
Microbiological Analysis ◽  
Local Markets ◽  
Baby Foods ◽  
Mesophilic Bacteria ◽  
Classical Culture

In this study, a total of 85 cereal-based baby foods with or without milk (four different brands; A, B, C, and D) collected from Ankara local markets, Turkey were analyzed for mycotoxins, total aerobic mesophilic bacteria (TAMB), and Enterobacteriaceae contamination. Baby foods were analyzed for 12 toxicological important mycotoxins such as aflatoxin B1, B2, G1, and G2; fumonisin B1 and B2; ochratoxin A; sterigmatocystin (STE); deoxynivalenol (DON); zearalenone (ZON); and T-2 toxin and HT-2 toxin by LC-MS/MS multi-mycotoxin method. In addition to these mycotoxins, the presence of aflatoxin M1 (AFM1) was investigated in baby foods containing milk. The classical culture method was used for microbiological analysis. Consequently, at least one mycotoxin was detected in 69.41% of the total samples. The most frequently detected mycotoxins were STE (34.12%) and HT-2 (34.12%). However, AFM1 was not detected in any of the baby foods containing milk. Also, TAMB and Enterobacteriaceae were isolated from 30.59% and 10.59% of samples, respectively. As a result, it was determined that the mycotoxin levels in the analyzed samples were in accordance with the mycotoxin levels specified in the Turkish Food Codex.

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