Further Studies on the Dissociation of Adult Mouse Tissue.

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

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Distribution of synuclein family mRNAs in adult mouse brain

Neuroscience Research ◽

10.1016/s0168-0102(00)81568-0 ◽

2000 ◽

Vol 38 ◽

pp. S120

Author(s):

A Ninomiya

Keyword(s):

Mouse Brain ◽

Adult Mouse ◽

Adult Mouse Brain

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Sox2 expression in the myenteric plexus in the caecum of postnatal and adult mouse

Zeitschrift für Gastroenterologie ◽

10.1055/s-0036-1587111 ◽

2016 ◽

Vol 54 (08) ◽

Author(s):

D Grundmann ◽

E Loris ◽

L Aigner ◽

S Couillard-Despres ◽

KH Schäfer

Keyword(s):

Myenteric Plexus ◽

Adult Mouse ◽

Sox2 Expression

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Activation of Factor X by Factor VIIa Complexed with Human-mouse Tissue Factor Chimeras Requires Human Exon 3

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650584 ◽

1996 ◽

Vol 76 (03) ◽

pp. 361-368 ◽

Cited By ~ 13

Author(s):

Carrie H Fang ◽

T-C Lin ◽

Arabinda Guha ◽

Yale Nemerson ◽

William H Konigsberg

Keyword(s):

Tissue Factor ◽

Human Factor ◽

Factor Viia ◽

Mouse Tissue ◽

Factor X ◽

Lower Affinity ◽

E Coli ◽

Sequence Elements ◽

Specific Activities ◽

Human And Mouse

SummaryIn an attempt to define sequence elements in human and mouse tissue factor (TF) that are responsible for the species specificity observed in their interaction with human factor VIIa (HVIIa), we constructed human-mouse chimeric TF cDNAs, inserted them into plasmid vectors, and induced their expression in E.coli. Assays for procoagulant activity were carried out with the resulting E. coli lysates using (HVIIa) human and mouse (MVIIa). The ratio of the procoagulant activities, HVIIa/MVIIa, revealed that human TF exon 3 was essential for activity when the TF:VIIa complex was formed with HVIIa. By ligating the maltose binding protein (MBP) gene to TF cDNAs it was possible to construct, express and purify MBP-TF chimeras as well as to estimate their specific activities. With selected MBP-TF chimeras and HVIIa we determined kinetic parameters for the activation of human factor X. Replacement of exon 3 in human TF cDNA with the corresponding exon from mouse TF cDNA resulted in both lower affinity for HVIIa and failure to convert bound HVIIa into a potent protease

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