scholarly journals HISTOLOGY OF BONE LESION AND CORRELATION OF CLINICAL, CLINICAL-LABORATORY DATA AND MORPHOLOGIC INDICES IN DESTRUCTIVE FORM OF OSTEOMYELITIS WITH LATENT COURSE (BRODIE’S ABSCESS)

2018 ◽  
Vol 25 (2) ◽  
pp. 47-55
Author(s):  
V. V. Grigorovskiy ◽  
N. P. Gritsai ◽  
A. S. Gordiy ◽  
O. B. Lyutko ◽  
A. V. Grigorovskaya
Keyword(s):  
Blood Cells ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Bone Lesion ◽  
Laboratory Data ◽  
Internal Capsule ◽  
Laboratory Methods ◽  
Brodie’S Abscess ◽  
Low Degree ◽  
Morphological Diagnosis

Urgency. Although the basic pathomorphologic features of bone lesion in destructive form of osteomyelitis with latent clinical course (Brodie’s abscess) are known from the literature, the thorough analytical clinical-morphologic studies have not been yet conducted.Purpose of study: Based on the results of the study to establish correlation dependence between clinical, clinical-laboratory indices and morphologic foci parameters.Material and methods. Fragments of pathologic foci tissues from 25 patients with Brodie’s abscess (31 samples) were the study material. The results of clinical, visualizing and clinical-laboratory methods as well as morphometric semi-quantitative indices characterizing the state of foci tissues were used for the gradationfrequency and correlation analysis.Results. In Brodie’s abscess the bone tissue morphologic changes are presented by spongiosa necrosis and resorption. More often the cases with granulation tissue within the internal capsule of a membrane prevailed. The evidence of exudative inflammation corresponded to the low degree of activity, of a productive one — to high activity. In the majority of cases small sequesters within the capsule tissues were detected. Reliable (p0.05) correlations that corresponded to absolute values of correlation coefficient ra in the range of 0.3 — 0.7 were determined for the following indices: “patient’s age” — “exudative inflammation within the capsule”, “white blood cells” — “pattern of capsule connective tissue”, “white blood cells” — “productive inflammation within the capsule”, “white blood cells” — “presence of sequesters”, “Antistaphylococcal antibodies” — “exudative inflammation within the capsule”.Conclusion. Determined clinical-morphologic correlations could be used to improve both clinical and morphological diagnosis of bone inflammatory lesions.

DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

2019 ◽  
pp. 10-13
Author(s):  
Ranu Kumar ◽  
Prasad Kapildeo
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Healthcare Services ◽  
Field Level ◽  
Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

The approximate formulas predicting personal somatic telomere length using patient blood test data

2019 ◽  
Vol 97 (11) ◽  
pp. 1090-1093
Author(s):  
Toyoki Maeda ◽  
Takahiko Horiuchi ◽  
Naoki Makino
Keyword(s):  
Telomere Length ◽  
Test Data ◽  
Blood Cells ◽  
Blood Test ◽  
White Blood Cells ◽  
Laboratory Data ◽  
Biological Aging ◽  
Differential Prediction ◽  
Test Items ◽  
Peripheral Leukocytes

Biological aging underlies lifestyle-related diseases. It can be assessed by measuring personal somatic cell telomere length. However, measuring the telomere length is laborious, and its clinical surrogate parameters have not been developed. This study analyzed the correlation between telomere length in peripheral leukocytes and laboratory data to select test items relating closely to biological aging. We established formulas from these clinical data to predict the personal telomere length. The subjects were patients having visited Kyushu University Beppu Hospital from 2012 to 2015. Two hundred and thirty-two patients were enrolled. The blood data were collected and telomere lengths were measured by Southern blotting method. The patients showed significant correlations between the telomere length and several blood test data with a sex-related difference. Candidate formulas are as follows: Predicted telomere length (kb) in men = 8.59 − 0.037 × Age (years) + 0.024 × Hemoglobin (g/dL); Predicted telomere length (kb) in women = 4.83 − 0.019 × Age (years) + 0.23 × Albumin (g/dL) + 0.0001 × White blood cells (/mm3) + 0.0020 × Red blood cells (× 104/mm3) + 0.0032 × Total cholesterol (mg/dL). Thus, the derived formulas allow for the accurate differential prediction of telomeric length in male and female patients.

Abstract WP31: Fibrin Rich Thrombus versus Erythrocyte Rich Thrombus in Acute Large Vessel Occlusion

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Kota Maekawa ◽  
Masunari Shibata ◽  
Masaru Seguchi ◽  
Kazuto Kobayashi ◽  
Hidetaka Nakajima ◽  
...  
Keyword(s):  
Blood Cells ◽  
Mechanical Thrombectomy ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Functional Independence ◽  
Large Vessel ◽  
Basilar Artery Occlusion ◽  
Large Vessel Occlusion ◽  
Vessel Occlusion ◽  
Significant Difference

Objective: The aim of this study was to evaluate thrombus composition and its association with clinical, laboratory, and neurointerventional findings in patients treated by mechanical thrombectomy due to acute large vessel occlusion. Methods: From August 2015 to June 2016, 72 patients were treated in our hospital by mechanical thrombectomy using stent retriever and/or aspiration catheter. Retrieved thrombi underwent semiquantitative analysis to quantify red blood cells, white blood cells, and fibrin by area. We divided patients into two groups as fibrin rich group or erythrocyte rich group according to predominant composition in thrombus. Two groups were compared with respect to imaging, clinical, and neurointerventional data. Results: Histopathologic analysis of retrieved thrombus from 37 patients with acute stroke due to internal carotid artery, middle cerebral artery, or basilar artery occlusion was performed. Erythrocyte rich thrombi were present in 13 (35%) of cases, and fibrin rich thrombi in 24 (65%). Cardioembolic etiology was significantly more in patients with fibrin rich thrombi than those with erythrocyte rich thrombi (79% vs. 38%; p=0.01). All other clinical and laboratory characteristics did not differ. Patients with fibrin rich thrombi had greater number of recanalization maneuvers (2.8 ± 1.2 vs. 1.8 ± 1.6, p=0.04) and longer interval time between puncture and recanalization (62 ± 33.6 minutes vs. 42 ± 21.3 minutes; p=0.04). There is no significant difference in occluded vessels and mechanical thrombectomy devices between two groups. Patients with fibrin rich thrombi were lower rate of functional independence (mRS score, 0-2) at 90 days (33% vs. 75%; p=0.04). Conclusion: This study showed that fibirin rich thrombus was associated with extended procedure time, unfavorable clinical outcome and cardioembolic etiology.

scholarly journals Bacteriological Characteristics of COVID-19 Patients Nosocomially Co-infected at a Designated Hospital: A Retrospective Study

2020 ◽  
Author(s):  
Jing Yu ◽  
Lei Nie ◽  
Xia Zhou ◽  
Dongde Wu ◽  
Jian Chen ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
White Blood Cells ◽  
Laboratory Data ◽  
Critical Factor ◽  
Severe Pneumonia ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Extensive Drug Resistance ◽  
Causative Agents ◽  
Secondary Bacterial Pneumonia

Abstract Background: Bacterial co-infection in patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a critical factor that increases the complexity and treatment of coronavirus disease 2019 (COVID-19). Methods: We collected the clinical laboratory data of 1799 patients with confirmed COVID-19 who were admitted to Jinyintan Hospital in Wuhan, China, between January 1 to April 26, 2020. The bacterial co-infection along with disease progression was analyzed. Other inflammatory markers, including C-reactive protein (CRP), white blood cells (WBC), lymphocytes (L), neutrocytes (N), interleukin-6 (IL-6), and procalcitonin (PCT), were assessed to estimate the progression of COVID-19. Results: We found that 191 of the 1799 (10.62%) patients had bacterial co-infection. The most prevalent causative agents for bacterial co-infection were Klebsiella pneumoniae (91 cases, 5.06%) and Acinetobacter baumannii (66 cases, 3.67%). The most patients with bacterial co-infection showed extensive drug-resistance. The outcomes of patients with bacterial co-infection were worse than those of patients without bacterial co-infection.Conclusions: Secondary bacterial pneumonia during virus infection is a major risk factor for high mortality resulting from severe pneumonia caused by COVID-19.

scholarly journals Erythrocyte-Rich Thrombus Is Associated with Reduced Number of Maneuvers and Procedure Time in Patients with Acute Ischemic Stroke Undergoing Mechanical Thrombectomy

2018 ◽  
Vol 8 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Kota Maekawa ◽  
Masunari Shibata ◽  
Hideki Nakajima ◽  
Akane Mizutani ◽  
Yotaro Kitano ◽  
...  
Keyword(s):  
Blood Cells ◽  
Mechanical Thrombectomy ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Artery Occlusion ◽  
Basilar Artery Occlusion ◽  
Time Interval ◽  
Procedure Time ◽  
Vessel Occlusion ◽  
Computed Tomography Images

Background: Only few studies have investigated the relationship between the histopathology of retrieved thrombi and clinical outcomes. This study aimed to evaluate thrombus composition and its association with clinical, laboratory, and neurointerventional findings in patients treated by mechanical thrombectomy due to acute large vessel occlusion. Methods: At our institution, 79 patients were treated by mechanical thrombectomy using a stent retriever and/or aspiration catheter between August 2015 and August 2016. The retrieved thrombi were quantitatively analyzed to quantify red blood cells, white blood cells, and fibrin by area. We divided the patients into two groups – a fibrin-rich group and an erythrocyte-rich group – based on the predominant composition in the thrombus. The groups were compared for imaging, clinical, and neurointerventional data. Results: The retrieved thrombi from 43 patients with acute stroke from internal carotid artery, middle cerebral artery, or basilar artery occlusion were histologically analyzed. Erythrocyte-rich thrombi were present in 18 cases, while fibrin-rich thrombi were present in 25 cases. A cardioembolic etiology was significantly more prevalent among the patients with fibrin-rich thrombi than among those with erythrocyte-rich thrombi. Attenuation of thrombus density as shown on computed tomography images was greater in patients with erythrocyte-rich thrombi than in those with fibrin-rich thrombi. All other clinical and laboratory characteristics remained the same. Patients with erythrocyte-rich thrombi had a smaller number of recanalization maneuvers, shorter procedure times, a shorter time interval between arrival and recanalization, and a higher percentage of stent retrievers in the final recanalization procedure. The occluded vessels did not differ significantly. Conclusions: In this study, erythrocyte-rich thrombus was associated with noncardioembolic etiology, higher thrombus density, and reduced procedure time.

scholarly journals Impact of Intervention on Reduction of White Blood Cells in the Management of Infection in Pneumonia Patients

2020 ◽  
Vol 8 (1) ◽  
pp. 22-24
Author(s):  
Sri Aryanda ◽  
Azizah Nasution ◽  
Khairunissa K
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Laboratory Data ◽  
Base Line ◽  
Paired Samples ◽  
Significant Difference ◽  
Before And After ◽  
The Mean ◽  
The Impact ◽  
Treatment Of Infection

Objective: To study the impact of intervention on the reduction of white blood cells (WBC) in the management of infection in patients with pneumonia in Universitas Sumatera Utara (USU) hospital period July through September 2019. Method: This three-month analytical prospective cohort study analyzed the reduction of WBC in the management of infection in pneumonia patients (n=42) before and after intervention in USU hospital. A self-designed questionair was used to assess the patients characterics, the required laboratory data including WBC, and length of stay (LOS). The base-line data were assessed after three days of treatment. The data with  intervetion were assessed between July - September 2019. The Patients characteristics and drug utilization were analyzed descriptively. The levels of WBC before and after interventions were compared using paired samples t test (p < 0.05) was considered significant. Results: Characteristics of the pneumonia patients: male, 61.9%; female, 38.1%. The mean age of patients with pneumonia was 60.48 ± 14.76 years. The most widely used antibiotic was ceftriaxone as much as 48%. There was a significant difference in reduction of WBC between patients with pneumonia before and after intervention, p= 0.002. Conclusions: Intervention can significantly increase the treatment of infection in patients with pneumonia.  

scholarly journals Malaria Parasite Density Estimated with White Blood Cells Count Reference Value Agrees with Density Estimated with Absolute in Children Less Than 5 Years in Central Ghana

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Dennis Adu-Gyasi ◽  
Kwaku Poku Asante ◽  
Sam Newton ◽  
Sabastina Amoako ◽  
David Dosoo ◽  
...  
Keyword(s):  
Reference Values ◽  
Parasite Density ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Geometric Mean ◽  
White Blood Cells ◽  
Laboratory Data ◽  
Age Groups ◽  
Reference Value ◽  
The Difference

Introduction. The estimation of malaria parasite density using a microscope heavily relies on White Blood Cells (WBCs) counts. An assumed WBCs count of 8000/µL has been accepted as reasonably accurate in estimating malaria parasite densities due to the challenge to accurately determine WBCs count. Method. The study used 4944 pieces of laboratory data of consented participants of age group less than 5 years. The study compared parasite densities of absolute WBCs, assumed WBCs, and the WBCs reference values in Central Ghana. Ethical approvals were given by three ethics committees. Results. The mean (±SD) WBCs and geometric mean parasite density (GMPD) were 10500/µL (±4.1) and 10644/µL (95% CI 9986/µL to 11346/µL), respectively. The difference in the GMPD compared using absolute WBCs and densities of assumed WBCs was significantly lower. The difference in GMPD obtained with an assumed WBCs count and that of the WBCs reference values for the study area, 10400/µL and 9200/µL for children in different age groups, were not significant. Discussion. Significant errors could result when assumed WBCs count is used to estimate malaria parasite density in children. GMPD generated with WBCs reference values statistically agreed with density from the absolute WBCs. When obtaining absolute WBC is not possible, the reference value can be used to estimate parasite density.

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

White blood cells levels and PCOS: direct and indirect relationship with insulin resistance, but not with hyperandogenemia

2013 ◽  
Author(s):  
Olga Papalou ◽  
Sarantis Livadas ◽  
Athanasios Karachalios ◽  
Nektarios Benetatos ◽  
George Boutzios ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Cells ◽  
White Blood Cells ◽  
Indirect Relationship
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