scholarly journals Uji Tuberkulosis Laten Pada Kontak Serumah Pasien BTA Positif Dengan Metode Mantoux Test

2019 ◽  
Vol 17 (1) ◽  
pp. 50-63
Author(s):  
Norma Tiku Kambuno ◽  
Yunita Helmina Senge ◽  
Agustina W. Djuma ◽  
Elisabeth N. Barung
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mantoux Test

Latar Belakang. Tuberkulosis adalah suatu penyakit infeksi yang disebabkan oleh bakteri Mycobacterium tuberculosis. Tuberkulosis laten adalah salah satu keadaan yang terjadi ketika terpapar dengan bakteri TB namun tidak menunjukan gejala klinis. Anggota keluarga rentan tertular TB dikarenakan sulit menghindari kontak dengan penderita. Uji tuberculin skin test adalah salah satu uji yang dipakai untuk mendiagnosa TB laten. Tujuan. Tujuan penelitian ini adalah untuk mengetahui gambaran TB laten pada kontak serumah dengan pasien TB BTA positif.  Menentukan hubungan antara TB laten dengan umur, jenis kelamin, pendidikan, pekerjaan dan status merokok. Metode.  Jenis penelitian yang digunakan adalah penelitian analitik korelasi dengan desain cros sectional dengan jumlah sampel sebanyak 22 orang. Hasil. Berdasarkan hasil penelitian  sebanyak 15 orang (68,2%) dengan indurasi > 10 mm, dan  7 orang (31, 8%) dengan indurasi < 10 mm . Sedangkan berdasarkan hasil uji statistik untuk melihat hubungan antara pengaruh umur, jenis kelamin, tingkat pendidikan, pekerjaan dan status merokok dengan status TB laten diperoleh approx signifikan > 0,05. Kesimpulan. Dengan demikian prevalensi TB laten pada kontak serumah pasien BTA positif sebesar 68,2% dan tidak terdapat hubungan antara umur, jenis kelamin, tingkat pendidikan, pekerjaan dan status merokok dengan status TB.

scholarly journals Comparison of Two Commercially Available Gamma Interferon Blood Tests for Immunodiagnosis of Tuberculosis

2007 ◽  
Vol 15 (1) ◽  
pp. 168-171 ◽  
Author(s):  
Jose Domínguez ◽  
Juan Ruiz-Manzano ◽  
Malú De Souza-Galvão ◽  
Irene Latorre ◽  
Celia Milà ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Latent Infection ◽  
Positive Tuberculin Skin Test ◽  
Gamma Interferon ◽  
Bcg Vaccination ◽  
Blood Tests

ABSTRACT We evaluated the T-SPOT.TB and Quantiferon-TB Gold In tube (QFN-G-IT) tests for diagnosing Mycobacterium tuberculosis infection. T-SPOT.TB was more sensitive than QFN-G-IT in diagnosing both active and latent infection. Both gamma interferon tests were unaffected by prior Mycobacterium bovis BCG vaccination. Among children who were not BCG vaccinated but had a positive tuberculin skin test, QFN-G-IT was negative in 53.3% of cases, and T-SPOT.TB was negative in 50% of cases.

scholarly journals Evaluation of Gamma Interferon Immune Response Elicited by the Newly Constructed PstS-1(285-374):CFP10 Fusion Protein To Detect Mycobacterium tuberculosis Infection

2014 ◽  
Vol 21 (4) ◽  
pp. 552-560 ◽  
Author(s):  
Leonardo Silva de Araujo ◽  
Fernanda Carvalho de Queiroz Mello ◽  
Nidai de Bárbara Moreira da Silva ◽  
Janaina Aparecida Medeiros Leung ◽  
Silvia Maria Almeida Machado ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Positive Tuberculin Skin Test ◽  
Gamma Interferon ◽  
Preventative Treatment ◽  
Mycobacterium Tuberculosis Infection ◽  
Content Type ◽  
Ifn Γ ◽  
First Time

ABSTRACTThe PstS1 antigen is highly immunogenic, principally when combined with CFP10 during both latent and active TB infection. In the present study, a selectedpstS1gene fragment was cloned, fused with CFP10, and expressed inEscherichia coli. The product [PstS-1(285-374):CFP10] was compared to the recombinant fused RD1 (region of deletion 1) protein (ESAT-6:CFP10) in detectingMycobacterium tuberculosisinfection in 108 recent contacts of pulmonary tuberculosis (TB) cases, considering a positive tuberculin skin test (TST) to be the baseline. The release of gamma interferon (IFN-γ) in 22-h whole-blood and 5-day lymphocyte stimulation assays primed with each antigen was determined. All contacts were clinically followed for up to 1 year, and 87% of the tuberculin skin test-positive (TSTpositive) patients accepted preventative treatment. Concerning the IFN-γ response to PstS-1(285-374):CFP10 in the 22-h and 5-day assays, a slight increase in contact-TSTpositivedetection was observed (23/54 and 26/54) compared to the level seen with the RD1 protein (18/54 and 24/54) whereas in the TSTnegativegroup, similarly lower numbers (≤5/48) of responders were achieved for both antigens, except for RD1 in the 5-day assay (8/48). By combining the IFN-γ responders to both antigens in the 5-day assays, slightly higher increases in positivity were found in the TSTpositive(32/54) and TSTnegative(10/48) groups. Two of 12 untreated TSTpositivecontacts progressed to active TB and were concordantly positive in all assays, except for one contact who lacked positivity in the RD1 5-day assay. We demonstrated for the first time that PstS-1(285-374):CFP10 slightly increased contact positivity and detection of active disease progression, suggesting its potential application as a TB infection marker.

scholarly journals Early clearance versus control: what is the meaning of a negative tuberculin skin test or interferon-gamma release assay following exposure to Mycobacterium tuberculosis?

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 664 ◽  
Author(s):  
Erin W. Meermeier ◽  
David M. Lewinsohn
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Gamma ◽  
Adaptive Immune Response ◽  
Interferon Gamma Release Assay ◽  
Adaptive Immune ◽  
Release Assay ◽  
Sterilizing Immunity ◽  
Preventative Vaccine

The elimination of tuberculosis (TB) cannot reasonably be achieved by treatment of individual cases and will require an improved vaccine or immunotherapy. A challenge in developing an improved TB vaccine has been the lack of understanding what is needed to generate sterilizing immunity against Mycobacterium tuberculosis (Mtb) infection. Several epidemiological observations support the hypothesis that humans can eradicate Mtb following exposure. This has been termed early clearance and is defined as elimination of Mtb infection prior to the development of an adaptive immune response, as measured by a tuberculin skin test or interferon-gamma release assay. Here, we examine research into the likelihood of and possible mechanisms responsible for early clearance in household contacts of patients with active TB. We explore both innate and adaptive immune responses in the lung. Enhanced understanding of these mechanisms could be harnessed for the development of a preventative vaccine or immunotherapy.

scholarly journals FAKTOR YANG BERHUBUNGAN DENGAN TUBERCULIN SKIN TEST POSITIF PADA PETUGAS KESEHATAN DI RUMAH SAKIT DI KOTA JEMBER

2020 ◽  
Vol 6 (2) ◽  
pp. 261-267
Author(s):  
Hamidah Retno Wardani ◽  
Ni Made Mertaniasih ◽  
Soedarsono Soedarsono
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
P Value ◽  
Cross Sectional

Tuberkulosis merupakan penyakit infeksi yang tidak hanya ada di komunitas tetapi juga di tempat kerja seperti pelayanan kesehatan. Petugas kesehatan di Rumah Sakit memiliki risiko tinggi terpajan droplet yang mengandung Mycobacterium tuberculosis. Penelitian ini bertujuan untuk mendeteksi positif tuberculin skin test pada petugas kesehatan di Rumah Sakit di Kota Jember. Penelitian ini menggunakan cross-sectional, pada bulan Januari sampai Maret 2020 di dua rumah sakit di kota jember. Masing-masing petugas kesehatan di unit perawatan TB dan unit perawatan non TB dievaluasi menggunakan Tuberculin skin test dengan cut-off ? 10 mm untuk positif tuberculin skin test. Deteksi positif tuberculin skin test terjadi pada 77 (60.2%) petugas kesehatan dari total 128 petugas kesehatan. Faktor risiko jenis kelamin laki-laki (p-value=0.329; OR=1.425; CI=0.6499-2.904), rentang usia (p-value=0.676; OR=0.740; CI=0.176-3.101), BMI (p-value=0.539), riwayat merokok (p-value=0.132; OR=2.059; CI=0.796-5.330), riwayat kontak TB di keluarga (p-value=0.481; OR=1.555; CI=0.452-5.348), riwayat bekerja di unit TB (p-value=0.692; OR=1.154; CI=0.568-2.344) tidak memiliki hubungan bermakna dengan reaksi tuberculin skin test positif. Reaksi tuberculin skin test positif dengan prevalensi yang tinggi terjadi pada petugas kesehatan yang berisiko meningkatkan risiko terjadinya TB aktif pada petugas kesehatan di Rumah Sakit di Jember. Dibutuhkan suatu program untuk mencegah terjadinya TB pada petugas kesehatan.      Kata kunci: Tuberculin skin test; petugas kesehatan; faktor risiko

scholarly journals Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test

2014 ◽  
Vol 109 (8) ◽  
pp. 999-1004
Author(s):  
Bosco Christiano Maciel da Silva ◽  
Maria Fernanda Rios Grassi ◽  
Raimundo Coutinho ◽  
Rita Elizabeth Moreira Mascarenhas ◽  
Viviana Nilla Olavarria ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculin Skin Test ◽  
Skin Test

Mantoux Test in Kawasaki Disease

PEDIATRICS ◽  
1996 ◽  
Vol 98 (1) ◽  
pp. 161-161
Author(s):  
Alberto Bertotto ◽  
Fabrizio Spinozzi ◽  
Maurizio Radicioni ◽  
Renato Vaccaro
Keyword(s):  
Kawasaki Disease ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Acute Phase ◽  
Mantoux Test ◽  
Skin Test Reactivity ◽  
Purified Protein Derivative ◽  
Characteristic Features ◽  
Early Manifestation

Localized erythema at the site of a previous bacillus Calmette-Guérin (BCG) inoculation is a relatively specific and early manifestation of Kawasaki disease (KD),1 and redness and crusting are characteristic features at the site of BCG immunization during the acute phase of the illness.2 With these findings in mind, we evaluated the tuberculin skin test reactivity of children with KD, as determined by a commercially available (Sclavo, Siena, Italy) and intradermally injected (5 TU) purified protein derivative (PPD).

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