scholarly journals GENETIC ANALYSIS OF THE TYPES OF DEVELOPMENT OF MUTANT LINES FROM COMMON WHEAT VARIETIES

REPORTS ◽  
2020 ◽  
Vol 6 (334) ◽  
pp. 22-28
Author(s):  
N. Sarybay ◽  
◽  
Zh. Zh. Chunetova ◽  
D. M. Iskakova ◽  
B.A. Zhumabaeva ◽  
...  

Among cereals, a special place is occupied by wheat as the main food of humanity and in improving the country's economy. The main task of breeding is to produce valuable wheat varieties that are stable to the unfavorable external environment of various natural climatic zones of Kazakhstan, consisting of a combination of valuable features. The prospects for the use of mutants in the process of hybridization are reflected in the achievements of world and domestic selection. The effect of various solutions of chemical compounds on the plant was observed from the first days during the growth of wheat sprouts. The growth of the first sprout of seeds treated with a certain solution of cadmium chloride and zinc chloride - 0.01%, the activity of cell division and structural disorders of chromosomes were manifested by variability in the varieties Kazakhstani3, Shagala, Zhenis and Lutescens 32. When treating dry wheat grain with a concentration of 0.1% cadmium chloride, it brought the growth of the Sprout to a lethal state, 0.01% inhibited the growth of the Sprout for 4 days, and in a 0.001% solution, the growth rate of the control grains was the same. On the contrary, a 0.1% vertical solution of zinc chloride inhibited the growth of the Sprout for 1 Week, 0.01% for three days. Among the studied concentrations, a 0.01% solution of cadmium chloride inhibited the growth of sprouts, causing chromosomal aberrations and morphological variability in cell division. Therefore, a concentration of 0.01% of cadmium salt was obtained as an optimal concentration to expand the limits of variability in wheat. In this regard, in our study, it was found that a 0.01% solution of cadmium chloride is an effective concentration that expands the limits of variability in wheat. The effect of this amount of solution leads to morphological changes in the plant (Binding of the stem, elongation of the earlobe, increase in the number and weight of grains in the headlobe, increase in the weight of 1000 grains, etc.), and changes in the characteristics of the M1 - M4 offspring are constantly inherited. Mutant lines L1, L2 and L3 differ in valuable breeding characteristics under the influence of a 0.01 percent solution of cadmium salt were obtained for the Kazakhstanskaya 3 and Shagala varieties. A genetic analysis of the type of development of these altered lines was carried out, as a result of which a long (16 cm) dense ear (0.80) changed from the Gull variety to a prismatic plant of the control variety (0.50), as a result of hybridization, its summer type (from the vrn gene) to the autumn type (Vrn gene). As a result of the study, Kazakhstanskaya 3, Kazakhstanskaya mutant 3 varieties contain the genes Vrn 1, Vrn 3. Well, it was found that Shagala varieties consist of the genes Vrnl, Vrn2. Varieties containing the genes Vrn1, Vrn3 show rapid maturation. However, they differed from each other in the period of intoxication.

Genetics ◽  
1980 ◽  
Vol 95 (3) ◽  
pp. 561-577 ◽  
Author(s):  
Steven I Reed

ABSTRACT Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle of Saccharomyces cereuisiae were isolated and subjected to preliminary characterization. Complementation studies assigned these mutations to four complementation groups, one of which, cdc28, has been described previously. Genetic analysis revealed that these complementation groups define single nuclear genes, unlinked to one another. One of the three newly identified genes, cdc37, has been located in the yeast linkage map on chromosome IV, two meiotic map units distal to hom2.—Each mutation produces stage-specific arrest of cell division at start, the same point where mating pheromone interrupts division. After synchronization at start by incubation at the restrictive temperature, the mutants retain the capacity to enlarge and to conjugate.


eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Michael J Prigge ◽  
Matthieu Platre ◽  
Nikita Kadakia ◽  
Yi Zhang ◽  
Kathleen Greenham ◽  
...  

The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.


1988 ◽  
Vol 89 (1) ◽  
pp. 39-47 ◽  
Author(s):  
C. Gonzalez ◽  
J. Casal ◽  
P. Ripoll

Mutation in the gene merry-go-round (mgr) of Drosophila causes a variety of phenotypic traits in somatic and germinal tissues, such as polyploid cells, metaphasic arrest, postmeiotic cysts with 16 nuclei, and spermatids with four times the normal chromosome content. The most characteristic phenotype is the appearance of mitotic and meiotic figures where all chromosomes are arranged in a circle. Treatment with anti-mitotic drugs and the phenotype of double mutants mgr asp (asp being a mutation altering the spindle) show that these circular figures need a functional spindle for their formation. These abnormal figures are caused by monopolar spindles similar to those observed after different treatments in several organisms. All mutant traits indicate that mgr performs a function necessary for the correct behaviour of centrosomes, thus opening this organelle to genetic analysis.


2021 ◽  
pp. 312-319
Author(s):  
Abdulwahid Saif ◽  
Aref Al-Shamiri ◽  
Abdulnour Shaher

Abstract M3 derived mutants from two bread wheat varieties, namely, 'Giza 186' and 'Saha 93', were screened for resistance to the rust Ug99 at two locations in Njoro (Kenya) and in Tihama (Yemen). At Tihama, two mutants of 'Giza 186' (G-M2-2010-1-28 and G-M2-2010-41-52) and four mutants of 'Saha 93' (S-M2-2010-16-12, S-M2-2010-21-13, S-M2-2010-22-14 and S-M2-2010-27-15) were seen to be resistant at both seedling and adult stages while their parents were resistant at seedling stage and susceptible at adult stage. In Kenya, the resistance score of the mutants was slightly different from those obtained at Tihama. The mutants G-M2-2010-1-28 and G-M2-2010-41-52 were stable in their level of resistance recorded at Tihama, but only two mutants of 'Saha 93' (S-M2-2010-16-12 and S-M2-2010-27-15) were resistant at both growth stages. S-M2-2010-22-14 and S-M2-2010-21-13 were resistant at the seedling stage while susceptible at adult stage. Further selection on these mutants for yield potential, agronomic performance and yellow rust disease resistance, as well as on selected mutants of both 'Giza 186' and 'Saha 93', at M5-M6 stages identified superior mutant lines compared with the two parents 'Saha 93' and 'Giza 186'. These included the line Erra-010-GM2w-41-52-40, which ranked first in yield (3768 kg/ha), followed by the lines Erra-010-SwM2-16-12-19, Erra-010-GM2w-1-28-18 and Erra-010-SwM2-22-14-6. Moreover, it can be concluded that Erra-010-GM2w-41-52-40 and Erra-010-SwM2-16-12-19 are highly recommended for their resistance to stem and yellow rust diseases as well as for yield potential and preference by farmers. Therefore, efforts are in progress to increase their seeds for dissemination over a wide range of farmers and wheat areas where rust diseases are an epidemic, and for registration of the lines as improved mutant varieties.


1967 ◽  
Vol 45 (24) ◽  
pp. 3203-3206 ◽  
Author(s):  
O. K. Srivastava ◽  
E. A. Secco

Infrared spectra and thermogravimetric (t.g.a.) and differential thermal (d.t.a.) analyses are reported for Cd(OH)2 obtained by the action of dilute ammonia solution on aqueous solutions of cadmium chloride, sulfate, and nitrate. The analyses indicate that the precipitated cadmium hydroxide from these solutions is often admixed with basic salts.


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