Bile Canaliculus

2020 ◽  
Author(s):  
Keyword(s):  
Bile Canaliculus

Sepsis and cholestasis: basic findings in the sinusoid and bile canaliculus

2001 ◽  
Vol 8 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Koichi Hirata ◽  
Shinichiro Ikeda ◽  
Toshio Honma ◽  
Toshihiro Mitaka ◽  
Tomohisa Furuhata ◽  
...  
Keyword(s):  
Bile Canaliculus

Annexin VI defines an apical endocytic compartment in rat liver hepatocytes

1998 ◽  
Vol 111 (2) ◽  
pp. 261-269 ◽  
Author(s):  
D. Ortega ◽  
A. Pol ◽  
M. Biermer ◽  
S. Jackle ◽  
C. Enrich
Keyword(s):  
Rat Liver ◽  
Bile Canaliculus ◽  
Polymeric Immunoglobulin Receptor ◽  
Receptor Recycling ◽  
Structural Protein ◽  
Immunoglobulin Receptor ◽  
Lysosomal Compartment ◽  
Endocytic Compartment ◽  
Apical Domain ◽  
Annexin Vi

Annexin VI has been demonstrated previously to be a marker for hepatic endosomes. By western blotting with an affinity purified anti-annexin VI antibody it was shown that annexin VI was present in the three morphologically and functionally different endosomal fractions from rat liver. We have quantified the gold-labeled endosomes by immunoelectron microscopy in ultrathin Lowicryl sections of rat liver and now demonstrate that 80% of the total labeling with anti-annexin VI was associated with endocytic structures surrounding the bile canaliculus, the apical domain of hepatocytes, whereas only 20% was found in the subsinusoidal endosomes. In double immuno-gold labeling experiments 80% of the Rab5 positive apical endosomes were also labeled with anti-annexin VI antibodies. However, there was no significant colocalization with antibodies to the polymeric immunoglobulin receptor. Finally, we demonstrate that 50% of endosomes containing internalized gold-labeled transferrin were double labeled with anti-annexin VI antibodies. Thus, annexin VI becomes the first known structural protein at the apical ‘early’ endocytic compartment of the hepatocyte that may be involved in the receptor recycling and transport to late endocytic/lysosomal compartment pathways.

Membrane Antigen Expression of Syngeneically but Heterotopically Transplanted Hepatocytes in Rats

1994 ◽  
Vol 3 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Y. Fujikura ◽  
H. Kuniki ◽  
T. Sawada ◽  
K. Hamano ◽  
T. Akino ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Antigen Expression ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Fatty Degeneration ◽  
Bile Canaliculus ◽  
Lymphoid Tissues ◽  
Fiber Network ◽  
Fetal Hepatocytes ◽  
Reticulin Fiber

The expression of membrane antigens on rat hepatocytes transplanted syngeneically and heterotopically was analyzed immunohistochemically using monoclonal antibodies against rat hepatocytes. Isolated adult and fetal hepatocytes were able to survive in the spleen, salivary gland, thymus, or subcapsular region of the kidney for various periods after transplantation. Fairly clear expression of HAM2, 4, and 8 antigens was observed on hepatocytes transplanted into syngeneic spleen, suggesting that the cells might be functionally equivalent to hepatocytes in situ. HAM4 antigen was localized specifically on the newly formed bile-canalicular faces of hepatocytes. The expression of HAM2 (MHC class I) antigen on the transplanted hepatocytes appeared much stronger on the side facing lymphoid tissues, than on the other faces, suggesting that some immunological reactions may take place between hepatocytes and lymphoid tissue. HAM8 antigen, which is localized on gap junctions between neighboring hepatocytes in rat liver, was also recognized between transplanted hepatocytes. In salivary glands where hepatocytes were transplanted, bile-canaliculus-like structures were observed not only between neighboring hepatocytes but also between hepatocytes and salivary acinar cells, suggesting good interaction between the two different epithelial cell types. Hepatocytes transplanted into thymus appeared viable, but most showed fatty degeneration. Some healthy hepatocytes survived in the interlobular connective tissue and the thymic cortical tissue. When fetal hepatocytes were transplanted heterotopically, they formed a mass consisting of hepatocytes and bile duct-like structures 7 wk after transplantation. The inoculated hepatocytes possessed HAM4 antigen, which was not recognized on fetal hepatocytes at day 14 of gestation. These results suggest that transplanted hepatocytes can grow in any syngeneic tissues, and that a common feature on such hepatocytes is a rich reticulin fiber network, visualized by silver staining. To judge the state of transplanted hepatocytes, monoclonal antibodies against rat hepatocyte surface antigens might therefore be a useful tool.

scholarly journals Partial characterization of mechanism(s) by which sulphobromophthalein reduces biliary lipid secretion

1993 ◽  
Vol 291 (1) ◽  
pp. 173-177 ◽  
Author(s):  
G Yamashita ◽  
S Tazuma ◽  
K Horikawa ◽  
N Aihara ◽  
H Ochi ◽  
...  
Keyword(s):  
Bile Salt ◽  
Sodium Taurocholate ◽  
Bile Canaliculus ◽  
Biliary Secretion ◽  
Dependent Manner ◽  
Biliary Lipid ◽  
Sprague Dawley ◽  
Lipid Secretion ◽  
Biliary Lipids ◽  
Sprague Dawley Rats

This study was performed to explore the mechanisms by which sulphobromophthalein (BSP) reduces the secretion of biliary lipid using Sprague-Dawley rats (SDR) and mutant rats with congenital conjugated hyperbilirubinaemia bred from SDR (EHBR). We infused the bile-salt-pool-depleted rats with sodium taurocholate at a constant rate of 160 nmol/min per 100 g body wt. with BSP (12.5, 25 and 50 nmol/min per 100 g body wt.) or BSP-GSH (12.5, 25 and 50 nmol/min per 100 g body wt.). The biliary secretion of BSP and BSP-GSH was markedly impaired in EHBR as compared with that in SDR. BSP reduced the biliary secretion of cholesterol and phospholipids in a dose-dependent manner without affecting the secretion of bile salts and composition of fatty acids in phospholipids in SDR, but had no effect on lipid secretion in EHBR. In contrast, BSP-GSH had no such effect on biliary lipids, either in the SDR or EHBR. In addition, the amount of BSP in the liver of EHBR was in the same range as that of SDR. Therefore it is unlikely that an intracellular mechanism is involved in the phenomenon of uncoupling by BSP. We conclude that the uncoupling of biliary lipids from bile-salt secretion by BSP occurs at the level of the bile canaliculus following the secretion of unconjugated BSP.

scholarly journals Intracellular and transcellular transport of secretory component and albumin in rat hepatocytes.

1983 ◽  
Vol 97 (5) ◽  
pp. 1582-1591 ◽  
Author(s):  
E S Sztul ◽  
K E Howell ◽  
G E Palade
Keyword(s):  
Membrane Proteins ◽  
Golgi Complex ◽  
Rat Hepatocytes ◽  
Secretory Component ◽  
Bile Canaliculus ◽  
Sds Page ◽  
Iga Antibody ◽  
Co Precipitation ◽  
Kinetics Of

The intra- and transcellular transports of hepatic secretory and membrane proteins were studied in rats in vivo using [3H]fucose and [35S]cysteine as metabolic precursors. Incorporated radioactivity in plasma, bile, and liver subcellular fractions was measured and the labeled proteins of the Golgi complex, bile, and plasma were separated by SDS PAGE and identified by fluorography. 3H-radioactivity in Golgi fractions peaked at 10 min postinjection (p.i.) and then declined concomitantly with the appearance of labeled glycoproteins in plasma. Maximal secretion of secretory fucoproteins from Golgi occurred between 10 and 20 min p.i. In contrast, the clearance of labeled proteins from Golgi membrane subfractions occurred past 30 min p.i., indicating that membrane proteins leave the Golgi complex at least 30 min later than the bulk of content proteins. A major 80,000-dalton form of secretory component (SC) was identified in the bile by co-precipitation with (IgA)2 by an anti-IgA antibody. An antibody (raised in rabbit) against the biliary 80,000-dalton peptide recognized two larger forms (116,000 and 94,000 dalton), presumably precursors, in Golgi membranes. A comparative study of kinetics of transport of 35S-SC and 35S-albumin showed that albumin peaked in bile at approximately 45 min p.i., whereas the SC peak occurred at 80 min p.i., suggesting that the transit time differs for plasma and membrane proteins that are delivered to the bile canaliculus.

scholarly journals Abnormal secretion of proteins into bile from colchicine-treated isolated perfused rat livers

1983 ◽  
Vol 216 (2) ◽  
pp. 409-414 ◽  
Author(s):  
S G Barnwell ◽  
R Coleman
Keyword(s):  
Serum Albumin ◽  
Protein Composition ◽  
Bile Canaliculus ◽  
Perfusion Fluid ◽  
Rat Serum ◽  
Essential Change ◽  
Rat Livers ◽  
Rat Bile ◽  
Rat Serum Albumin ◽  
Microtubular System

The microtubule poison, colchicine, caused an abnormal output of a variety of proteins into rat bile. After 3 h of exposure to the drug, livers were isolated and perfused with media of defined protein composition. There was no essential change in permeability of the hepatobiliary system to proteins (e.g. bovine serum albumin) entering bile from the perfusion fluid. The rat (serum) albumin and fibrinogen that were secreted into bile from colchicine-treated livers were probably derived from the hepatocytes. Disruption of the microtubular system reduces the secretion of proteins at the sinusoidal face of the hepatocyte and results in an accumulation of secretory vesicles in the cytoplasm. It is suggested that under these conditions some of the vesicles discharge their contents into the bile canaliculus.

scholarly journals Deciphering Tumour Tissue Organization by 3D Electron Microscopy and machine learning

2021 ◽  
Author(s):  
Baudouin Denis de Senneville ◽  
Fatma Zohra Khoubai ◽  
Marc Bevilacqua ◽  
Alexandre Labedade ◽  
Kathleen Flosseau ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Machine Learning ◽  
Spatial Organization ◽  
Hot Spot ◽  
Bile Canaliculus ◽  
Tumour Cells ◽  
Blood Capillary ◽  
Tissue Organization ◽  
3D Electron Microscopy ◽  
Block Face

Despite recent progress in the characterization of tumour components, the tri-dimensional (3D) organization of this pathological tissue and the parameters determining its internal architecture remain elusive. Here, we analysed the spatial organization of patient-derived xenograft tissues generated from hepatoblastoma, the most frequent childhood liver tumour, by serial block-face scanning electron microscopy using an integrated workflow combining 3D imaging, manual and machine learning-based semi-automatic segmentations, mathematics and infographics. By digitally reconstituting an entire hepatoblastoma sample with a blood capillary, a bile canaliculus-like structure, hundreds of tumour cells and their main organelles (e.g. cytoplasm, nucleus, mitochondria), we report unique 3D ultrastructural data about the organization of tumoral tissue. We found that the size of hepatoblastoma cells correlates with the size of their nucleus, cytoplasm and mitochondrial mass. We also discovered that the blood capillary controls the planar alignment and size of tumour cells in their 3D milieu. Finally, a set of tumour cells polarized in the direction of a hot spot corresponding to a bile canaliculus-like structure. In conclusion, this pilot study allowed the identification of bioarchitectural parameters that shape the internal and spatial organization of tumours, thus paving the way for new investigations in an emerging field that we call onconanotomy.

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