Epstein-Barr Nuclear Antigen 2

USP7 (also called HAUSP) is a de-ubiquitinating enzyme recently identified as a key regulator of the p53–mdm2 pathway, which stabilizes both p53 and mdm2. We have discovered that the Epstein–Barr nuclear antigen 1 protein of Epstein–Barr virus binds with high affinity to USP7 and disrupts the USP7–p53 interaction. The results have important implications for the role of Epstein–Barr nuclear antigen 1 in the cellular immortalization that is typical of an Epstein–Barr virus latent infection.

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Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana

Viruses ◽

10.3390/v12070766 ◽

2020 ◽

Vol 12 (7) ◽

pp. 766

Author(s):

Richmond Ayee ◽

Maame Ekua Oforiwaa Ofori ◽

Emmanuel Ayitey Tagoe ◽

Sylvester Languon ◽

Kafui Searyoh ◽

...

Keyword(s):

Epstein Barr Virus ◽

Venous Blood ◽

Nasopharyngeal Cancer ◽

Nuclear Antigen ◽

Control Group ◽

Barr Virus ◽

Genotype 2 ◽

Epstein Barr ◽

Study Participants ◽

Epstein Barr Nuclear Antigen

Nasopharyngeal cancer (NPC) is associated with Epstein Barr virus (EBV) infection. However different viral strains have been implicated in NPC worldwide. This study aimed to detect and characterize EBV in patients diagnosed with NPC in Ghana. A total of 55 patients diagnosed with NPC by CT scan and endoscopy were age-matched with 53 controls without a known oncological disease. Venous blood was collected from the study participants and DNA extracted from the blood samples. Detection of EBV and genotyping were done by amplifying Epstein Barr nuclear antigen 1 (EBNA-1) and Epstein Barr nuclear antigen 2 (EBNA-2), respectively, using specific primers. Viral load in patients and controls was determined using real-time polymerase chain reaction. EBV positivity in controls (92%) was significantly greater than that of NPC patients (67%) (χ2 = 19.17, p < 0.0001), and viral infection was independent of gender (χ2 = 1.770, p = 0.1834). The predominant EBV genotypes in patients and controls were genotype 2 (52%) and genotype 1 (62%), respectively. Median EBV load was significantly higher in NPC patients than the control group (p < 0.01). In summary, prevalence of EBV genotype 2 infection was higher in NPC patients than the control group. Assessment of EBV load may be used as a biomarker for the diagnosis of NPC.

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Cytotoxic effector cells specific for Epstein-Barr nuclear antigen positive nasopharyngeal hybrid cells are present in patients with infectious mononucleosis.

Nippon Jibiinkoka Gakkai Kaiho ◽

10.3950/jibiinkoka.91.1831 ◽

1988 ◽

Vol 91 (11) ◽

pp. 1831-1836

Author(s):

MITSURU FURUKAWA ◽

MICHIHIRO KAMIDE ◽

CHIIKO IKEDA ◽

HIDEO SAKASITA ◽

RYOZO UMEDA

Keyword(s):

Infectious Mononucleosis ◽

Nuclear Antigen ◽

Hybrid Cells ◽

Effector Cells ◽

Epstein Barr ◽

Epstein Barr Nuclear Antigen ◽

Cytotoxic Effector

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The interaction between smoking and Epstein-Barr virus as multiple sclerosis risk factors may depend on age

Multiple Sclerosis Journal ◽

10.1177/1352458513507820 ◽

2013 ◽

Vol 20 (6) ◽

pp. 747-750 ◽

Cited By ~ 15

Author(s):

Jonatan Salzer ◽

Hans Stenlund ◽

Peter Sundström

Keyword(s):

Risk Factors ◽

Multiple Sclerosis ◽

Epstein Barr Virus ◽

Nuclear Antigen ◽

Positive Interaction ◽

Barr Virus ◽

Older Subjects ◽

Epstein Barr ◽

A Prospective Study ◽

Epstein Barr Nuclear Antigen

The multiple sclerosis (MS) risk factors smoking and remote Epstein-Barr virus (EBV) infection have been suggested to interact statistically, but the results are conflicting. In a prospective study on 192 MS cases and 384 matched controls, we analysed levels of cotinine as a marker of smoke exposure, and Epstein-Barr Nuclear Antigen-1 antibody reactivity. We assessed interaction on the additive and multiplicative scales, and estimated the effects of the risk factors across strata of each other. The results suggest that a negative interaction may be present in samples drawn at a young age, and a positive interaction among older subjects.

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Epigenetic reprogramming sensitizes immunologically silent EBV+ lymphomas to virus-directed immunotherapy

Blood ◽

10.1182/blood.2019004126 ◽

2020 ◽

Vol 135 (21) ◽

pp. 1870-1881 ◽

Cited By ~ 1

Author(s):

Tanner Dalton ◽

Ekaterina Doubrovina ◽

Dmitry Pankov ◽

Raymond Reynolds ◽

Hanna Scholze ◽

...

Keyword(s):

T Cell ◽

Cytotoxic T Cells ◽

Nuclear Antigen ◽

Potent Inducer ◽

Barr Virus ◽

Cell Immunotherapy ◽

T Cell Homing ◽

Inhibition Of Tumor Growth ◽

Epstein Barr ◽

Epstein Barr Nuclear Antigen

Abstract Despite advances in T-cell immunotherapy against Epstein-Barr virus (EBV)-infected lymphomas that express the full EBV latency III program, a critical barrier has been that most EBV+ lymphomas express the latency I program, in which the single Epstein-Barr nuclear antigen (EBNA1) is produced. EBNA1 is poorly immunogenic, enabling tumors to evade immune responses. Using a high-throughput screen, we identified decitabine as a potent inducer of immunogenic EBV antigens, including LMP1, EBNA2, and EBNA3C. Induction occurs at low doses and persists after removal of decitabine. Decitabine treatment of latency I EBV+ Burkitt lymphoma (BL) sensitized cells to lysis by EBV-specific cytotoxic T cells (EBV-CTLs). In latency I BL xenografts, decitabine followed by EBV-CTLs results in T-cell homing to tumors and inhibition of tumor growth. Collectively, these results identify key epigenetic factors required for latency restriction and highlight a novel therapeutic approach to sensitize EBV+ lymphomas to immunotherapy.

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