scholarly journals Activating de novo triacylglycerol synthesis in oleaginous yeast for improved bio-diesel quality

2018 ◽  
Vol 4 (2) ◽  
pp. 16-24
Author(s):  
Farha Deeba ◽  
Ruchir Priyadarshi ◽  
Vikas Pruthi ◽  
Yuvraj Singh Negi
Keyword(s):  
Fatty Acid ◽  
Sodium Bicarbonate ◽  
Acid Content ◽  
Oleaginous Yeast ◽  
De Novo ◽  
Fatty Acid Content ◽  
Biodiesel Production ◽  
Nitrogen Base ◽  
Triacylglycerol Synthesis ◽  
Tag Accumulation

The economic production of yeast biofuels requires several strategies, such as multiomics techniques to gain insights into lipid biosynthesis pathway. Hence, metabolomic studies for amelioration of triacylglycerol (TAG) accumulation in oleaginous yeast strain to reveal potential targets in fatty acid synthesis pathway should be explored. The aim of this research experiment was to boost TAG agglomeration in novel isolated oleaginous yeast Cryptococcus psychrotolerans IITRFD using sodium bicarbonate as supplement for biodiesel production. Enhanced biomass productivity (83.5 ± 0.32 mg/L/h) and lipid productivity (56.8 ± 0.34 mg/L/h) have been estimated as compared to yeast nitrogen base media (YNB) used as control. Also, the bicarbonate supplementation (25 mM) leads to decrease in phospholipids (23.6 %) while increase in TAG amount (75.4 %) signifying that it redirects the phospholipids synthesis pathway in the direction of de novo TAG pathway. The fatty acid profile obtained revealed rise in monounsaturated fatty acid content and decrease in polyunsaturated fatty acid content demonstrating better oxidative stability (19 h) and cold flow behaviour (- 12 °C) of biodiesel produced. This novel strategy of utilizing bicarbonate as a triggering system may possibly revamp the commercial use of C. psychrotolerans IITRFD for high quality biodiesel production. This is the first study to augment TAG accumulation in this oleaginous yeast using sodium bicarbonate with improved fuel properties.

Two-step in situ biodiesel production from microalgae with high free fatty acid content

2013 ◽  
Vol 136 ◽  
pp. 8-15 ◽  
Author(s):  
Tao Dong ◽  
Jun Wang ◽  
Chao Miao ◽  
Yubin Zheng ◽  
Shulin Chen
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acid Content ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Biodiesel Production ◽  
High Free Fatty Acid

Study of biodiesel production from animal fats with high free fatty acid content

2011 ◽  
Vol 102 (23) ◽  
pp. 10907-10914 ◽  
Author(s):  
J.M. Encinar ◽  
N. Sánchez ◽  
G. Martínez ◽  
L. García
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acid Content ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Biodiesel Production ◽  
High Free Fatty Acid ◽  
Animal Fats

scholarly journals Biodiesel production from waste fish oil with high free fatty acid content from Moroccan fish-processing industries

2018 ◽  
Vol 27 (2) ◽  
pp. 249-255 ◽  
Author(s):  
K. Kara ◽  
F. Ouanji ◽  
El M. Lotfi ◽  
M. El Mahi ◽  
M. Kacimi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fish Oil ◽  
Free Fatty Acid Content ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Biodiesel Production ◽  
High Free Fatty Acid ◽  
Fish Processing

Fatty acid content and composition of sediments from Siberian eutrophic water bodies: Implications for biodiesel production

2013 ◽  
Vol 47 (9) ◽  
pp. 3192-3200 ◽  
Author(s):  
Nadezhda N. Sushchik ◽  
Anna Yu Kuchkina ◽  
Michail I. Gladyshev
Keyword(s):  
Fatty Acid ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Water Bodies ◽  
Biodiesel Production ◽  
Eutrophic Water

Polymorphisms in genes in the SREBP1 signalling pathway and SCD are associated with milk fatty acid composition in Holstein cattle

2011 ◽  
Vol 79 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Gonzalo Rincon ◽  
Alma Islas-Trejo ◽  
Alejandro R Castillo ◽  
Dale E Bauman ◽  
Bruce J German ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Candidate Genes ◽  
Acid Content ◽  
Milk Fat ◽  
De Novo ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Signalling Pathway ◽  
Phenotypic Variance

Genes in the sterol regulatory element-binding protein-1 (SREBP1) pathway play a central role in regulation of milk fat synthesis, especially the de-novo synthesis of saturated fatty acids. SCD, a SREBP-responsive gene, is the key enzyme in the synthesis of monounsaturated fatty acids in the mammary gland. In the present study, we discovered SNP in candidate genes associated with this signalling pathway and SCD to identify genetic markers that can be used for genetic and metabolically directed selection in cattle. We resequenced six candidate genes in the SREBP1 pathway (SREBP1, SCAP, INSIG1, INSIG2, MBTPS1, MBTPS2) and two genes for SCD (SCD1 and SCD5) and discovered 47 Tag SNP that were used in a marker-trait association study. Milk and blood samples were collected from Holstein cows in their 1st or 2nd parity at 100–150 days of lactation. Individual fatty acids from C4 to C20, saturated fatty acid (SFA) content, monounsaturated fatty acid content, polyunsaturated fatty acid content and desaturase indexes were measured and used to perform the asociation analysis. Polymorphisms in the SCD5 and INSIG2 genes were the most representative markers associated with SFA/unsaturated fatty acid (UFA) ratio in milk. The analysis of desaturation activity determined that markers in the SCD1 and SCD5 genes showed the most significant effects. DGAT1 K232A marker was included in the study to examine the effect of this marker on the variation of milk fatty acids in our Holstein population. The percentage of variance explained by DGAT1 in the analysis was only 6% of SFA/UFA ratio. Milk fat depression was observed in one of the dairy herds and in this particular dairy one SNP in the SREBP1 gene (rs41912290) accounted for 40% of the phenotypic variance. Our results provide detailed SNP information for key genes in the SREBP1 signalling pathway and SCD that can be used to change milk fat composition by marker-assisted breeding to meet consumer demands regarding human health, as well as furthering understanding of technological aspects of cows' milk.

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