scholarly journals EXPERIMENTAL STUDIES ON THE DETECTION AND QUANTIFICATION OF VOLATILE FLAMMABLE AND TOXIC SUBSTANCES IN THE AIR AND BIOLOGICAL FLUIDS OF THE ORGANISM

Fire Safety ◽  
2018 ◽  
pp. 74-79
Author(s):  
O. Scherbyna
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Biological Fluids ◽  
Experimental Studies ◽  
High Sensitivity ◽  
Absolute Calibration ◽  
Toxic Substances ◽  
Chromatography Method ◽  
Ultraviolet Detector

The methods of isolation, purification and qualitative and quantitative determination of phenol in air and biological fluids of an organism are offered. Isolation was carried out by distillation with water vapor, purification by extraction with chloroform, and identification by qualitative reactions, by chromatography in a thin layer of sorbent and by liquid chromatography. The quantitative determination of phenol was carried out by liquid chromatography in a reverse phase (chromatograph "Tsvet-304" with an ultraviolet detector). Calculation of the quantitative content of phenol was carried out by the method of absolute calibration. As a result of the research, it was found that by using the molecular liquid chromatography method, it is possible to determine 17-20% of phenol isolated from urine and 21-24% isolated from the air. The retention time of phenol 1h.14s, the results of the analysis are well reproducible. The worked out techniques have high resolution, high sensitivity and speed of analysis.

scholarly journals A SIMPLE REVERSE PHASE-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN IN BULK AND IT’S TABLET DOSAGE FORM

2017 ◽  
Vol 10 (12) ◽  
pp. 333
Author(s):  
Ramreddy Godela ◽  
Sherisha Bhavani
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Performance ◽  
Dosage Form ◽  
Reverse Phase ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Tablet Dosage

Objective: The most important objective of the present research work is to develop simple, specific, rapid, accurate, and sensitive reverse-phase high-performance liquid chromatography method and validated for the qualitative and quantitative determination of valsartan in its active pharmaceutical ingredient and tablet dosage form according to ICH guidelines.Proposed Method: An isocratic separation was done using Phenomenex C18 column possess 75×4.6 mm, 2.6 μ,100 A0 dimensions with mobile phase composition of water:acetonitrile (30:70% v/v) by maintaining 1 ml/minute flow rate and response detected at a wavelength of 247 nm.Results: The retention time of valsartan was found to be 2.71 minutes, limit of detection and limit of quantification were observed at 1.24 μg/ml and 3.6 μg/ml concentration, respectively, and a calibration curve was linear in the concentration range of 5-50 μg/ml with coefficient of correlation 0.99. The percentage recovery (accuracy) was in the range of 98.9-102%, and the % relative standard deviation was observed to be <2%.Conclusion: The proposed method was validated for accuracy, precision, sensitivity, linearity, and robustness and successfully employed for the quantitative determination of valsartan in tablet dosage form in quality control department of pharmaceutical industry.

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