scholarly journals Effectivity of Streptococcus agalactiae bacterial vaccine with different coatings for increasing the immunity system on nile tilapia Oreochromis niloticus (Linnaeus, 1758)

2019 ◽  
Vol 19 (2) ◽  
pp. 205
Author(s):  
Ricko Reynalta ◽  
Munti Yuhana ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Survival Rate ◽  
Antibody Titre ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Protein Concentration ◽  
Effective Control ◽  
Chitosan Coating ◽  
Nile Tilapia Oreochromis Niloticus

Vaccination is one of the most effective control measure in preventing Streptococcus agalactiae infection. In this study, vaccine coatings were prepared with certain biomaterials such as chitosan, skim milk, and maltodextrin at concentration 1 % or 10 %, and further freeze-dried. Vaccine selected (in vitro) by tests: viability cell, solubility, protein concentration and protein molecular weight. The result of in vitro test showed that chitosan coating at doses 1 % and 10 % were the best in solubility and protein concentration test. Vaccine coated with chitosan was administrated again by injection (in vivo) in nile tilapia (Oreochromis niloticus) before challenged by S. agalactiae. This test consists of six treatments and three replications, i.e. the fish were injected with liquid vaccine (A), Phospat Buffered Saline (PBS) (B), vaccine with chitosan coating 1 % (C), vaccine with chitosan coating 10 % (D), chitosan 1 % (E), and chitosan 10 % (F). Parameters were observed including survival rate, relative percent survival (RPS), antibody titre, and total leucocyte. The results showed that treatment C showed a significant difference (P < 0,05) in survival rate 92.22 ± 3.85 %, RPS 85.21 ± 7.20 %, and antibody titre highest result, but not significant (P > 0,05) to increase total leukocyte in experimental fish against S. agalactiae infection. In conclusion, vaccine freeze dry with chitosan coated 1 % is effective to improve immunity system of nile tilapia.

In vitro and in vivo screening of herbal extracts against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)

Aquaculture ◽  
2019 ◽  
Vol 503 ◽  
pp. 412-421 ◽  
Author(s):  
Wei-Liang Guo ◽  
Heng-Wei Deng ◽  
Fei Wang ◽  
Shi-Feng Wang ◽  
Zhi-Hong Zhong ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Herbal Extracts ◽  
In Vivo Screening ◽  
Nile Tilapia Oreochromis Niloticus

The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

2014 ◽  
pp. 27
Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Disease ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Ages ◽  
Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

Nonlethal sampling methods for diagnosis of Streptococcus agalactiae infection in Nile tilapia, Oreochromis niloticus (L.)

Aquaculture ◽  
2016 ◽  
Vol 454 ◽  
pp. 237-242 ◽  
Author(s):  
Guilherme Campos Tavares ◽  
Frederico Augusto de Alcântara Costa ◽  
Raquel Ribeiro Dias Santos ◽  
Gustavo Morais Barony ◽  
Carlos Augusto Gomes Leal ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Sampling Methods ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Dietary Agaricus blazei Spent Substrate Improves Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae In Vivo

2022 ◽  
Vol 10 (1) ◽  
pp. 100
Author(s):  
Po-Tsang Lee ◽  
Yu-Sheng Wu ◽  
Chung-Chih Tseng ◽  
Jia-Yu Lu ◽  
Meng-Chou Lee
Keyword(s):  
Cell Line ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Water Extract ◽  
Feed Additive ◽  
Agaricus Blazei ◽  
Bacterial Challenge ◽  
Spent Mushroom Substrate ◽  
Nile Tilapia Oreochromis Niloticus

This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.

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