DAYA HAMBAT PERASAN JERUK NIPIS (Citrus aurantifolia) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis

Mirza Aryanto

doi:10.32509/jitekgi.v17i1.1071

DAYA HAMBAT PERASAN JERUK NIPIS (Citrus aurantifolia) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis

Jurnal Ilmiah dan Teknologi Kedokteran Gigi ◽

10.32509/jitekgi.v17i1.1071 ◽

2021 ◽

Vol 17 (1) ◽

pp. 16-19

Author(s):

Mirza Aryanto

Keyword(s):

Enterococcus Faecalis ◽

Citrus Aurantifolia

Perawatan saluran akar merupakan salah satu perawatan endodontik untuk mempertahankan gigi dengan bagian jaringan pulpa yang sudah terinfeksi bakteri. Irigasi saluran akar merupakan salah satu proses yang sangat penting untuk mengeliminasi bakteri dalam saluran akar. Pada saluran akar gigi dengan perawatan endodontik yang gagal ditemukan bakteri Enterococcus faecalis, sehingga diperlukan alternatif bahan irigasi yang lebih efektif. Tujuan : Menjelaskan daya hambat perasan jeruk nipis (Citrus aurantifolia) sebagai bahan alternatif larutan irigasi dalam menghambat pertumbuhan bakteri Enterococcus faecalis pada perawatan saluran akar. Bahan dan Metode : Sampel yang diuji berjumlah 32 berupa biakan bakteri Enterococcus faecalis dalam media agar BHI pada cawan petri. Penelitian ini menggunakan metode difusi cakram kertas. Setiap 1 cawan petri dibagi menjadi 4 bagian dan diletakkan cakram kertas yang masing-masing telah diberikan perasan jeruk nipis (Citrus aurantifolia) kosentrasi 100 % dan kontrol positif (NaOCl 2,5%). Bakteri ditanam dan diinkubasi, kemudian diencerkan dengan standar konsentrasi bakteri McFarland I (1.10 6 cfu/ml). Hasil Penelitian : Besar rerata daya hambat bakteri Enterococcus faecalis dalam perasan jeruk nipis (Citrus aurantifolia) sebesar 5,8 1,8 mm, sedangkan pada larutan NaOCl 2,5% sebesar 3,9 1,1 mm. Uji hipotesis memiliki nilai p = 0,000 (p 0,05). Kesimpulan : Perasan jeruk nipis (Citrus aurantifolia) memiliki daya hambat bakteri Enterococcus faecalis yang lebih efektif.

Download Full-text

Differences in effectiveness of guava leaf extract (Psidium guajava linn) and lime water (Citrus aurantifolia) as irrigation material of root canal as inhibitors of bacteria Enterococcus faecalis (Laboratory of Microbiology Faculty of Pharmacy University

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v3i3.780 ◽

2018 ◽

Vol 3 (3) ◽

pp. 156

Author(s):

Syamsiah Syam ◽

Nur F. Arifin ◽

Nurkhairah Y. Irkan

Keyword(s):

Enterococcus Faecalis ◽

Root Canal ◽

Leaf Extract ◽

Psidium Guajava ◽

Citrus Aurantifolia ◽

Lime Water ◽

Guava Leaf

Download Full-text

Uji Efektivitas Ekstrak Buah Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Bakteri Enterococcus faecalis Secara In Vitro

Andalas Dental Journal ◽

10.25077/adj.v3i2.109 ◽

2015 ◽

Vol 3 (2) ◽

pp. 111-118

Author(s):

Annisa Fajriatul Arafah ◽

Vivi Triana ◽

Murniwati Murniwati

Keyword(s):

Citric Acid ◽

Enterococcus Faecalis ◽

Root Canal ◽

Bacterial Cell ◽

Early Stage ◽

Inhibition Zone ◽

Diffusion Method ◽

Control Group ◽

Citrus Aurantifolia

Enterococcus faecalis is a normal microorganism in root canal teeth, anaerobic facultative, and gram positive cocci. This bacteria is opportunistic that cause secunder infection in root canal. Lime (Citrus aurantifolia) is one of the plants which have antimicrobial activity because lime contains flavonoid and citric acid. Flavonoids can denature proteins in bacterial cell and damaging the cell membrane. Citric acid can damaging the bacterial cell wall and inhibiting bacterial enzymes activity. The purpose of this study was to examie the effectiveness lime extract in various concentration in inhibiting Enterococcus faecalis in in-vitro. The research method was experimental laboratories with posttest only control group design. Samples were used in this research are Enterococcus faecalis bacteria in M. Djamil’s Hospital Microbiology Laboratory, Padang. This research was conducted at organic chemistry of natural materials laboratory FMIPA UNAND and Kopertis Wilayah X Padang laboratory. Early stage was done by making extracts of lime with concentrations are 25%, 50%, 75%, and 100%. Then, the extracts was tested into the bacteria with the diffusion method used papper disk. The inhibition zone formed between lime extracts in various concentrations to the growth of Enterococcus faecalis were calculated using calipers. Data was analyzed by using Kruskal Wallis test and Mann-Whithney test with confidence interval of 95%. The result presented that 25%, 50%, 75%, and 100% lime extracts have inhibition activity in inhibiting the growth of Enterococcus faecalis, and the higher the concentration of the extracts, the greater the inhibition zone formed.

Download Full-text